Enter the position


Example input
More Parameters

Examples:

Type Chromosome Start position Editing pattern
Substitution chr1 943995 /T
Insertion chr1 943995 +GTATT
Deletion chr1 943995 -GAGAACTCGG

Above examples represent the input formats of three different editing types. The user should enter chromosome, starting position, and the editing pattern.

In these examples, the 943995th base in chr1 is base 'C', and the next 20 consecutive bases are 'GAGAACTCGGCACAGGAGAG').

  • The editing pattern begins with '/' ,'+' or '-' ,followed by the specific base sequence.

  • Substitution is indicated by backslash "/", and the example is used to replace C with T.

  • Insertion is indicated by plus sign "+", and the example indicates that GTATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GAGAACTCGG will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAS Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology
    Use an example: Substitution Insertion Deletion
    Chromosome
    Start position
    Editing pattern
    Example for BED file
    HISTORY
    UID Chromosome Position EDIT_PATTERN PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240205172520_ueem chr9 133748283 /T NGG 10 8 17 8 24 0 100 5 10
    240123090842_6k6y chr9 35063005 /C NGG 10 8 17 8 24 0 100 7 30
    240123090658_apkn chr9 35063005 /C NGG 10 12 16 10 12 0 100 5 10
    240122200836_byij chr1 943995 +GTATTTGAGT NGG 10 8 17 8 24 0 100 5 10
    240122200737_6txe chr1 943995 +GTATTCCCGC NGG 10 8 17 8 24 0 100 5 10
    240122130632_r1gl chr5 1278780 /T NGG 10 8 17 8 24 0 100 5 10
    240122095828_hyhq chr14 24240636 /T NGG 10 8 17 8 24 0 100 5 10
    240121051026_etf8 chr9 35063005 /C NGG 10 12 16 10 12 0 100 5 10
    240121050806_p7sf chr9 35063005 /C NGG 10 8 17 8 24 0 100 5 10
    240105135306_i2cy chr2 178461089 /A NGG 10 8 17 8 24 0 100 5 10