Enter the position


Example input
More Parameters

Examples:

Type Chromosome Start position Editing pattern
Substitution chr1 943995 /T
Insertion chr1 943995 +GTATT
Deletion chr1 943995 -GAGAACTCGG

Above examples represent the input formats of three different editing types. The user should enter chromosome, starting position, and the editing pattern.

In these examples, the 943995th base in chr1 is base 'C', and the next 20 consecutive bases are 'GAGAACTCGGCACAGGAGAG').

  • The editing pattern begins with '/' ,'+' or '-' ,followed by the specific base sequence.

  • Substitution is indicated by backslash "/", and the example is used to replace C with T.

  • Insertion is indicated by plus sign "+", and the example indicates that GTATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GAGAACTCGG will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAS Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology
    Use an example: Substitution Insertion Deletion
    Chromosome
    Start position
    Editing pattern
    Example for BED file
    HISTORY
    UID Chromosome Position EDIT_PATTERN PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240527121846_po4p chr2 12951321 /C NGG 10 8 17 8 24 0 100 5 10
    240527121740_a8jk chr1 943995 /T NGG 10 8 17 8 24 0 100 5 10
    240402230257_ga0t chr7 96121734 /A NGG 10 8 17 8 24 0 100 5 10
    240308093126_p8dr chr1 113337067 +CCGGATGATCCTGACGACGGAGACCGCCGTCGTCGACAAGCCGGCC NGG 20 8 17 8 82 0 100 5 10
    240308093113_gwp9 chr1 113338067 +CCGGATGATCCTGACGACGGAGACCGCCGTCGTCGACAAGCCGGCC NGG 20 8 17 8 82 0 100 5 10
    240308093054_j7h8 chr1 113343067 +CCGGATGATCCTGACGACGGAGACCGCCGTCGTCGACAAGCCGGCC NGG 20 8 17 8 82 0 100 5 10
    240308093042_suxn chr1 113342067 +CCGGATGATCCTGACGACGGAGACCGCCGTCGTCGACAAGCCGGCC NGG 20 8 17 8 82 0 100 5 10
    240308093012_acx4 chr1 113341067 +CCGGATGATCCTGACGACGGAGACCGCCGTCGTCGACAAGCCGGCC NGG 2000 8 17 8 82 0 100 5 10
    240308083453_1rdp chr1 113339961 +CCGGATGATCCTGACGACGGAGACCGCCGTCGTCGACAAGCCGGCC NGG 15 8 17 8 82 0 100 5 10
    240308083340_lp3p chr1 113339961 +CCGGATGATCCTGACGACGGAGACCGCCGTCGTCGACAAGCCGGCC NGG 100 8 20 8 90 0 100 5 10