Enter the position


Example input
More Parameters

Examples:

Type Chromosome Start position Editing pattern
Substitution chr1 943995 /T
Insertion chr1 943995 +GTATT
Deletion chr1 943995 -GAGAACTCGG

Above examples represent the input formats of three different editing types. The user should enter chromosome, starting position, and the editing pattern.

In these examples, the 943995th base in chr1 is base 'C', and the next 20 consecutive bases are 'GAGAACTCGGCACAGGAGAG').

  • The editing pattern begins with '/' ,'+' or '-' ,followed by the specific base sequence.

  • Substitution is indicated by backslash "/", and the example is used to replace C with T.

  • Insertion is indicated by plus sign "+", and the example indicates that GTATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GAGAACTCGG will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAS Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology
    Use an example: Substitution Insertion Deletion
    Chromosome
    Start position
    Editing pattern
    Example for BED file
    HISTORY
    UID Chromosome Position EDIT_PATTERN PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240308083340_lp3p chr1 113339961 +CCGGATGATCCTGACGACGGAGACCGCCGTCGTCGACAAGCCGGCC NGG 100 8 20 8 90 0 100 5 10
    240308083044_ydb5 chr19 5400761 +CCGGATGATCCTGACGACGGAGACCGCCGTCGTCGACAAGCCGGCC NGG 100 8 17 8 90 0 100 5 100
    240308082524_1m35 chr19 5400761 +CCGGATGATCCTGACGACGGAGACCGCCGTCGTCGACAAGCCGGCC NGG 100 8 17 8 90 0 100 5 30
    240308082402_0eup chr18 56534775 +CCGGATGATCCTGACGACGGAGACCGCCGTCGTCGACAAGCCGGCC NGG 100 8 17 8 82 0 100 5 10
    240308082208_kvfi chr18 56534775 +CCGGATGATCCTGACGACGGAGACCGCCGTCGTCGACAAGCCGGCC NGG 10 8 17 8 82 0 100 5 10
    240308081906_bkjw chr19 55112144 +CCGGATGATCCTGACGACGGAGACCGCCGTCGTCGACAAGCCGGCC NGG 10 8 18 8 90 0 100 5 20
    240228004726_tr4s chrX 106839363 /C NG 10 8 17 8 24 0 100 5 10
    240228004656_chy1 chrX 106873759 /T NGG 10 8 17 8 24 0 100 5 10
    240227235019_1r2t chrX 106873759 /T NGG 10 8 17 8 24 0 100 5 10
    240209061321_x8ux chr14 24240636 /T NGG 10 8 17 8 24 0 100 5 10