Enter the position


Example input
More Parameters

Examples:

Type Chromosome Start position Editing pattern
Substitution chr1 943995 /T
Insertion chr1 943995 +GTATT
Deletion chr1 943995 -GAGAACTCGG

Above examples represent the input formats of three different editing types. The user should enter chromosome, starting position, and the editing pattern.

In these examples, the 943995th base in chr1 is base 'C', and the next 20 consecutive bases are 'GAGAACTCGGCACAGGAGAG').

  • The editing pattern begins with '/' ,'+' or '-' ,followed by the specific base sequence.

  • Substitution is indicated by backslash "/", and the example is used to replace C with T.

  • Insertion is indicated by plus sign "+", and the example indicates that GTATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GAGAACTCGG will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAS Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology
    Use an example: Substitution Insertion Deletion
    Chromosome
    Start position
    Editing pattern
    Example for BED file
    HISTORY
    UID Chromosome Position EDIT_PATTERN PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240813142819_s09k32vi chr17 57675057 /T NGG 10 8 17 8 24 0 100 5 10
    240813142819_s09k0tup chr14 81143693 /T NGG 10 8 17 8 24 0 100 5 10
    240813140426_ts1k chr1 943995 /T NGG 10 8 17 8 24 0 100 5 10
    240730012307_w5q9vxvh chr1 943995 -GAGAACTCGG NGG 10 8 17 8 24 0 100 5 10
    240730012307_w5q9e27i chr1 943995 /T NGG 10 8 17 8 24 0 100 5 10
    240730012307_w5q9ab1c chr1 943995 +ATT NGG 10 8 17 8 24 0 100 5 10
    240704040930_crjg chr19 38510670 /A NGG 10 8 17 8 24 0 100 5 10
    240704021049_h623 chr19 38510670 /A NGG 10 8 17 8 24 0 100 5 10
    240704021003_n223 chr19 38510670 /A NGG 10 8 17 8 24 0 100 5 10
    240613221255_72zi chr1 30936663 /A NGG 10 8 17 8 24 0 100 5 10