Enter the position


Example input
More Parameters

Examples:

Type Chromosome Start position Editing pattern
Substitution chr1 943995 /T
Insertion chr1 943995 +GTATT
Deletion chr1 943995 -GAGAACTCGG

Above examples represent the input formats of three different editing types. The user should enter chromosome, starting position, and the editing pattern.

In these examples, the 943995th base in chr1 is base 'C', and the next 20 consecutive bases are 'GAGAACTCGGCACAGGAGAG').

  • The editing pattern begins with '/' ,'+' or '-' ,followed by the specific base sequence.

  • Substitution is indicated by backslash "/", and the example is used to replace C with T.

  • Insertion is indicated by plus sign "+", and the example indicates that GTATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GAGAACTCGG will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAS Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology
    Use an example: Substitution Insertion Deletion
    Chromosome
    Start position
    Editing pattern
    Example for BED file
    HISTORY
    UID Chromosome Position EDIT_PATTERN PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240813144823_39jw7un8 chr10 43124937 /C NGG 10 13 13 13 18 0 100 5 20
    240813144823_39jw5574 chr22 36306555 /T NGG 10 13 13 13 18 0 100 5 20
    240813144823_39jw1v37 chr21 39393246 /T NGG 10 13 13 13 18 0 100 5 20
    240813144823_39jw0xwx chr16 2054376 /A NGG 10 13 13 13 18 0 100 5 20
    240813144823_39jw08yb chr14 81143693 /T NGG 10 13 13 13 18 0 100 5 20
    240813142819_s09kzz1m chr10 43124937 /C NGG 10 8 17 8 24 0 100 5 10
    240813142819_s09ky45o chr1 193147965 /T NGG 10 8 17 8 24 0 100 5 10
    240813142819_s09kt2s3 chr22 36306555 /T NGG 10 8 17 8 24 0 100 5 10
    240813142819_s09kr727 chr4 6301106 /T NGG 10 8 17 8 24 0 100 5 10
    240813142819_s09kqh42 chr21 39393246 /T NGG 10 8 17 8 24 0 100 5 10