Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    260120205655_cldg GGGGCGGCTGGGCAACGACCCCACGCGGCGACGGCCCCCCCCCGCCCCGCAGATGAGGAGCAGCTCTA(/G)GGCCGAAGTGTCGCAGGCCGGGACCGTCCTGGA NGG 10 8 17 8 24 0 100 5 10
    260120175526_l3ce GAAGAGACGCCCCTGATCCCTCAGCGTTCATGCAGCCTCTTGTCCACGGAGGCTGGTGCCCTGCATGTGCTGCTGCCCGCTCGGGGCCCCGGGCCCCCCCAGCG(/A)CCTATCTTTCTCCTTTGGGGACCACTTGGCTGAGGACCTGTGCGTGCAGGCTGCCAAGGCCAGCG NGG 10 8 17 8 24 0 100 5 10
    260119163728_g4tl tccctgacacataaaaggtgtctttctgtcttgtatcctttggatatgggcatgtcagtttcatagggaaattttcacatggagcttttgtatttctttctttgccagtacaactgcatgtggtagcacactgtttaatcttttctcaaataaaaagacatggggcttcatttttgttttgcctttttggtatcttacagGAACTCCAGGATG(+C)GCATTGGGCAGCGGCAAACTGTTGTCAGAACATTGAATGCAACTGGGGAAGAAATAATTCAGCAATCCTCAAAAACAGATGCCAGTATTCTACAGGAAAAATTGGGAAGCCTGAATCTGCGGTGGCAGGAGGTCTGCAAACAGCTGTCAGACAGAAAAAAGAGgtagggcgacagatctaataggaatgaaaacattttagcagactttttaagctttctttagaagaatatttcatgagagattataagcagggtgaaaggcactaacattaaagaacctatcaac NGG 10 8 17 8 24 0 100 5 10
    260119163631_36fj CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    260119110327_1qfe tgtggatgggccattaatgccactggttctgctcattttgggga(/g)aggaacagggcccatttggctggatgagataaactgtaatggaaaagaatctcata NGG 10 8 17 8 24 0 100 5 10
    260119104829_aba5 gagggaactggacacatttggcttgacagtgtttcttgccatggaca(/g)cgagtctgctctctggcagtgtagacaccatgaatggggaaagcattattgca NGG 10 8 17 8 24 0 100 5 10
    260119103148_c7uj gagggaactggacacatttggcttgacagtgtttcttgccatggaca(/g)cgagtctgctctctggcagtgtagacaccatgaatggggaaagcattattgca NGG 10 8 17 8 24 0 100 5 10
    260119102831_oh2n gcttgacagtgtttcttgccatggaca(/g)cgagtctgctctctggcagtgtagacaccatga NGG 10 8 17 8 24 0 100 5 10
    260119102649_2n74 gcttgacagtgtttcttgccatggac(/g)acgagtctgctctctggcagtgtagacaccatga NGG 10 8 17 8 24 0 100 5 10
    260119102458_tdom gagggaactggacacatttggcttgacagtgtttcttgccatggac(/g)acgagtctgctctctggcagtgtagacaccatgaatggggaaagcattattgca NGG 10 8 17 8 24 0 100 5 10