Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250512155723_am0g CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250512000943_a550 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250508064756_dikd GGCCCTTTTCCAGGTTAGATGGTTGCTAACCTCGGAGCAGTCTATTTTCCCGCCTTTTCCGCGCCCAGCAGCAGCTTCCTTTGCCCCTCCCAACTTCCAC(/G)CCTGCGCGCAGTCGCAGATCGCCGCTGCTGTCCGTCCACCCGCGCCCCCTCAAAGCTTCCCCTAGCAACAAGCTCGGGCTCAGAGCGAAGGCGGGGCGAG NGG 10 8 17 8 24 0 100 5 10
    250506165049_0bwb TGACAAGAAGGCCAAGATTGCAACACCGTCAGGCCAGAAGACAGGTGACAAGAAGGGAGCTGTCCATGTGGCGACTCCTCACCCGGCCAAGAAGGCAGGCAAGACCCCGGCTACCAGCGACAAGTCGCCC(+AAGTCTG)AAGTCTGGAGGGTCGGTCGCGTGCAAGTCGTGCAGCAAGACATTCAACAGCGAGGGCGCTCTAGCTTCCCACTCCAAGGCAAAGCACGAGGCCAAGTGA NGG 10 8 17 8 24 0 100 5 10
    250506162814_p5cw TGACAAGAAGGCCAAGATTGCAACACCGTCAGGCCAGAAGACAGGTGACAAGAAGGGAGCTGTCCATGTGGCGACTCCTCACCCGGCCAAGAAGGCAGGCAAGACCCCGGCTACCAGCGACAAGTCGCCCAAGTCTG(+AAGTCTG)GAGGGTCGGTCGCGTGCAAGTCGTGCAGCAAGACATTCAACAGCGAGGGCGCTCTAGCTTCCCACTCCAAGGCAAAGCACGAGGCCAAGTGA NGG 10 8 17 8 24 0 100 5 10
    250504180349_qmrl CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250502024900_fxva CCAGCACTTCCTCAAGACGCCCCTGGAGAAGAATGCCCCCGTCCTGCTGGCTCTACTGGGCATCTGGTACATCAACTGCTACGGCTGTGAGACCCACGCCTTGCTGCCCTATGACCAGTACATGCACCGCTTTGCTGCCTATTTCCAGCA(/G)GGGTGACATGGAGTCCAACGGAAAGTACATCACCAAGTCCGGGGCCCGTGTGGACCACCAGACAGGCCCCATCGTGTGGGGGGAACCAGGGACCAATGGTCAACATGCATTCTACCAGCTCATCCACCAAGGCACCAAGATGAT NGG 10 8 17 8 24 0 100 5 10
    250502024131_z61l ACCAGCACTTCCTCAAGACGCCCCTGGAGAAGAATGCCCCCGTCCTGCTGGCTCTACTGGGCATCTGGTACATCAACTGCTACGGCTGTGAGACCCACGCCTTGCTGCCCTATGACCAGTACATGCACCGCTTTGCTGCCTATTTCCAGCA(/G)GGGTGACATGGAGTCCAACGGAAAGTACATCACCAAGTCCGGGGCCCGTGTGGACCACCAGACAGGCCCCATCGTGTGGGGGGAACCAGGGACCAATGGTCAACATGCATTCTACCAGCTCATCCA NGG 10 8 17 8 24 0 100 5 10
    250502023858_c6l0 ACCAGCACTTCCTCAAGACGCCCCTGGAGAAGAATGCCCCCGTCCTGCTGGCTCTACTGGGCATCTGGTACATCAACTGCTACGGCTGTGAGACCCACGCCTTGCTGCCCTATGACCAGTACATGCACCGCTTTGCTGCCTATTTCCAGCA(/G)GGGTGACATGGAGTCCAACGGAAAGTACATCACCAAGTCCGGGGCCCGTGTGGACCACCAGACAGGCCCCATCGTGTGGGGGGAACCAGGGACCAATGGTCAACATGCATTCTACCAGCTCATCCA NGG 10 8 17 8 24 0 100 5 10
    250502023818_nn7d ACCAGCACTTCCTCAAGACGCCCCTGGAGAAGAATGCCCCCGTCCTGCTGGCTCTACTGGGCATCTGGTACATCAACTGCTACGGCTGTGAGACCCACGCCTTGCTGCCCTATGACCAGTACATGCACCGCTTTGCTGCCTATTTCCAGCA(/G)GGGTGACATGGAGTCCAACGGAAAGTACATCACCAAGTCCGGGGCCCGTGTGGACCACCAGACAGGCCCCATCGTGTGGGGGGAACCAGGGACCAATGGTCAACATGCATTCTACCAGCTCATCCA NGG 10 8 17 8 24 0 100 5 10