Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    260303145036_tmla CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    260302212119_z1t6 GGGGCGGCTGGGCAACGACCCCACGCGGCGACGGCCCCCCCCCGCCCCGCAGATGAGGAGCAGCTCTA(/G)GGCCGAAGTGTCGCAGGCCGGGACCGTCCTGGA NGG 10 8 17 8 24 0 100 5 10
    260302201030_3zbw CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    260302201001_15zt CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    260302150445_pn28 aataaattagcatctcagaggctagaaaccgtccaatactgctgtgtccttcccaagggagctggggaggggaccctgggtcctggttggccacacagcctcttt(-aaagtg)ctgaagcccacagacagacagatgcccctgcctgctctctgg NG 10 8 17 8 24 0 100 5 10
    260301164950_cfps gagagtggaagaccagtccatct(/G)ggcccagcatggatgatgatgaagaggagtctggg NGG 10 8 17 8 24 0 100 5 10
    260227173912_yg9p GCTGCCCCAGAATCCAGTCTAA(/G)TCACCTTCGAGGCAGCTCCCCCGACGCTG NGG 10 8 17 8 24 0 100 5 10
    260227173119_y1b2 GCTGCCCCAGAATCCAGTCTAA(/G)TCACCTTCGAGGCAGCTCCCCCGACGCTG NGG 10 8 17 8 24 0 100 5 10
    260227173036_uo4r CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    260226133843_i7st GTAAGGACTTCCCTTAATGTCTGTCCGCTCACCTGAAGTGTTATAAGTAGGCTAAACAGGATAAACACAAAGTATATATTTCTGATTAATATTCATAGGATTCTTACCTGCT(+ctt)ATATCTCTCTGTTTTGTTTTTCTCCTTCATCATATTTTAG NGG 10 8 17 8 24 0 100 5 10