Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250605134434_e35j agtttgagagccggctggcggatgcgctgcaggaactgT(/C)gggcccagcatgaggaccaggtggagcagtataagaaggagctggagaagacttattctgc NGG 10 10 16 10 20 0 100 5 300
    250605134410_0l01 agtttgagagccggctggcggatgcgctgcaggaactgT(/C)gggcccagcatgaggaccaggtggagcagtataagaaggagctggagaagacttattctgc NGG 10 10 16 10 20 0 100 5 100
    250605133918_bow9 agtttgagagccggctggcggatgcgctgcaggaactgT(/C)gggcccagcatgaggaccaggtggagcagtataagaaggagctggagaagacttattctgc NGG 10 8 17 8 24 0 100 5 10
    250604233633_ay3v Agtattgttaaccttgcagaatggtcgatgtagggggcca(/c)aaggtcagagagaagaaaatggatacactgctttgaaaat NGG 10 8 17 8 24 0 100 5 10
    250528025208_sgep CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 30 8 17 8 35 0 100 5 10
    250528024840_f4w8 agtattgttaaccttgcagaatggtcgatgtagggggcca(/c)aaggtcagagagaagaaaatggatacactgctttgaaaat NGG 10 8 17 8 24 0 100 5 10
    250527235909_0zhp tatagggagacccaagcttgccaccATGgacatctccaagtttgcagagaaagacaatatagttcttt(/g)gagaaggtggaatcacactgagtggaggtcaacgagcaagaatttctttagcaagg NG 10 8 20 6 24 0 100 5 20
    250527210209_jyr1 GGGCCACCTCAAGGCCTGTTTGTCTGTTGCTTGATGCAGTCTGCGCCAGGACCCTCCCCTTCCCTGTGGTGCTGCGACCCTTATAATGAGCCTTCTTGCTTTACTCATAGGTGGGAAACTACCTCCG(/A)TATGTTCCGAGGTTCTCTGTACAAGAGATACCCCTCACTCTGGAGGCGACTAGCCACTGTGGAAGAGAGGAAGAAAATAGTTGCATCGTCACATGGTAAAAAAACAAAACCTAACACTAAGGGTGCGT NGG 10 8 17 8 24 0 100 5 10
    250527210156_19ya GGGCCACCTCAAGGCCTGTTTGTCTGTTGCTTGATGCAGTCTGCGCCAGGACCCTCCCCTTCCCTGTGGTGCTGCGACCCTTATAATGAGCCTTCTTGCTTTACTCATAGGTGGGAAACTACCTCCG(/A)TATGTTCCGAGGTTCTCTGTACAAGAGATACCCCTCACTCTGGAGGCGACTAGCCACTGTGGAAGAGAGGAAGAAAATAGTTGCATCGTCACATGGTAAAAAAACAAAACCTAACACTAAGGGTGCGT NGG 10 8 17 8 24 0 100 5 10
    250527194033_xakv GGGCCACCTCAAGGCCTGTTTGTCTGTTGCTTGATGCAGTCTGCGCCAGGACCCTCCCCTTCCCTGTGGTGCTGCGACCCTTATAATGAGCCTTCTTGCTTTACTCATAGGTGGGAAACTACCTCCG(/A)TATGTTCCGAGGTTCTCTGTACAAGAGATACCCCTCACTCTGGAGGCGACTAGCCACTGTGGAAGAGAGGAAGAAAATAGTTGCATCGTCACATGGTAAAAAAACAAAACCTAACACTAAGGGTGCGT NGG 10 8 17 8 24 0 100 5 10