Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    261023031656_lvr6 ACAGTGGGCTGCCTGGGGGGCTGATGCCACCATTCCAGGAGCCTCGGTGAAGAGAGGATATCCATCTGTGTAGCCGCTTCTCTATACGGGATTCCAGCTCCATGGCAGCCCGTCTGCTCCTCCTGGGCATCCTTCTCCTGCTGCTGCCCCTGCCCGTCCCTGCCCCGTGCCACACAGCCGCACGCTCAGAGTGCAAGCGCAGCCACAAGTTCGTGCCTGGTGCATGGCTGGCCGGGGAGGGTGTGGACGTGACCAGCCTCCGCCGCTCGGGCTCCTTCCCAGTGGACACACAAAGGTTCCTGCGGCCCGACGGCACCTGCACCCTCTGTGAAAATGCCCTACAGGAGGGCACCCTCCAGCGCCTGCCTCTGGCGCTCACCAACTGGCGGGCCCAGGGCTCTGGCTGCCAGCGCCATGTAACCAGGGCCAAAGTCAGCTCCACTGAAGCTGTGGCCCGGGATGCGGCTCGTAGCATCCGCAACGACTGGAAGGTCGGGCTGGACGTGACTCCTAAGCCCACCAGCAATGTGCATGTGTCTGTGGCCGGCTCACACTCACAGGCAGCCAACTTTGCAGCCCAGAAGACCCACCAGGACCAGTACAGCTTCAGCACTGACACGGTGGAGTGCCGCTTCTACAGTTTCCATGTGGTACACACTCCCCCGCTGCACCCTGACTTCAAGAGGGCCCTCGGGGACCTGCCCCACCACTTCAACGCCTCCACCCAGCCCGCCTACCTCAGGCTTATCTCCAACTACGGCACCCA(/G)CTTCATCCGGGCTGTGGAGCTGGGTGGCCGCATATCGGCCCTCACTGCCCTGCGCACCTGCGAGCTGGCCCTGGAAGGGCTCACGGACAACGAGGTGGAGGACTGCCTGACTGTCGAGGCCCAGGTCAACATAGGCATCCACGGCAGCATCTCTGCCGAAGCCAAGGCCTGTGAGGAGAAGAAGAAGAAGCACAAGATGACGGCCTCCTTCCACCAAACCTACCGGGAGCGCCACTCGGAAGTGGTTGGCGGCCATCACACCTCCATTAACGACCTGCTGTTCGGGATCCAGGCCGGGCCCGAGCAGTACTCAGCCTGGGTAAACTCGCTGCCCGGCAGCCCTGGCCTGGTGGACTACACCCTGGAACCCCTGCACGTGCTGCTGGACAGCCAGGACCCGCGGCGGGAGGCACTGAGGAGGGCCCTGAGTCAGTACCTGACGGACAGGGCTCGCTGGAGGGACTGCAGCCGGCCGTGCCCACCAGGGCGGCAGAAGAGCCCCCGAGACCCATGCCAGTGTGTGTGCCATGGCTCAGCGGTCACCACCCAGGACTGCTGCCCTCGGCAGAGGGGCCTGGCCCAGCTGGAGGTGACCTTCATCCAAGCATGGGGCCTGTGGGGGGACTGGTTCACTGCCACGGATGCCTATGTGAAGCTCTTCTTTGGTGGCCAGGAGCTGAGGACGAGCACCGTGTGGGACAATAACAACCCCATCTGGTCAGTGCGGCTGGATTTTGGGGATGTGCTCCTGGCCACAGGGGGGCCCCTGAGGTTGCAGGTCTGGGATCAGGACTCTGGCAGGGACGATGACCTCCTTGGCACCTGTGATCAGGCTCCCAAGTCTGGTTCCCATGAGGTGAGATGCAACCTGAATCATGGCCACCTAAAATTCCGCTATCATGCCAGGTGCTTGCCCCACCTGGGAGGAGGCACCTGCCTGGACTATGTCCCCCAAATGCTTCTGGGGGAGCCTCCAGGAAACCGGAGTGGGGCCGTGTGGTGAGAACAGTGAGCTTGGAAAGGACCAGTATGCTTGGACTGAAGGGGTTCTCACAGTGGGAGCCAGGGCTGTCTTCGTATTCCCATTAGACCAAGCTTGTCCAACCCGAGGCCCGCATGCGGCCCAGGATGGCTTTGAATGCGGCCCAACGCAAATTCGCAAACTTTCTTAAAACATTATGAGTTTCTTTTTGCTATTTTTTTTTTTTTTTTAGCTCATCGGCTATCGTTAGTGCTAGTGGATTTTACATGTGGCCCAACACAATTCTTCTTCCAACGTGGCCCAGAGAAGCCAAAAGATTGGATACGCATCAGACAGATGGAAAAGGGAGATTCAGACTGTTTTTCAGGGAGGTGGCTGGGTTTACACGCTAATCCCGATTCACCCTGTCCAAACTGCCTAAGCCCTCCGCCATTCTCAAGCCCTGCAGTCACAGCTACACAGATCACAGCTTCAGCCAGGAGCTGGGCAGAAGGCCAAGAGGCTGTTCCCACCAGGCTGCTCAGGGCTGGTCTTTTAGGACCCTTCCCTTGAGCCCTCTATGGTGTGGCAAAGCCTTCATTGCCTTAACTGGAGCCCCATCAGCTCCAGCTGCTCTGTCTTCTTTGCCCACAATGCTTTGCCCCTGAGACAAATGGAGGCCTGTCCTGACCTGTCTCACCATGTACATAGCTTGATAAAGGGCCAATAAATATGATGTTATGGTGA NGG 10 8 17 8 24 0 100 5 10
    261023031034_yelt CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    261023025036_g5ll ACAGTGGGCTGCCTGGGGGGCTGATGCCACCATTCCAGGAGCCTCGGTGAAGAGAGGATATCCATCTGTGTAGCCGCTTCTCTATACGGGATTCCAGCTCCATGGCAGCCCGTCTGCTCCTCCTGGGCATCCTTCTCCTGCTGCTGCCCCTGCCCGTCCCTGCCCCGTGCCACACAGCCGCACGCTCAGAGTGCAAGCGCAGCCACAAGTTCGTGCCTGGTGCATGGCTGGCCGGGGAGGGTGTGGACGTGACCAGCCTCCGCCGCTCGGGCTCCTTCCCAGTGGACACACAAAGGTTCCTGCGGCCCGACGGCACCTGCACCCTCTGTGAAAATGCCCTACAGGAGGGCACCCTCCAGCGCCTGCCTCTGGCGCTCACCAACTGGCGGGCCCAGGGCTCTGGCTGCCAGCGCCATGTAACCAGGGCCAAAGTCAGCTCCACTGAAGCTGTGGCCCGGGATGCGGCTCGTAGCATCCGCAACGACTGGAAGGTCGGGCTGGACGTGACTCCTAAGCCCACCAGCAATGTGCATGTGTCTGTGGCCGGCTCACACTCACAGGCAGCCAACTTTGCAGCCCAGAAGACCCACCAGGACCAGTACAGCTTCAGCACTGACACGGTGGAGTGCCGCTTCTACAGTTTCCATGTGGTACACACTCCCCCGCTGCACCCTGACTTCAAGAGGGCCCTCGGGGACCTGCCCCACCACTTCAACGCCTCCACCCAGCCCGCCTACCTCAGGCTTATCTCCAACTACGGCACCCA(/G)CTTCATCCGGGCTGTGGAGCTGGGTGGCCGCATATCGGCCCTCACTGCCCTGCGCACCTGCGAGCTGGCCCTGGAAGGGCTCACGGACAACGAGGTGGAGGACTGCCTGACTGTCGAGGCCCAGGTCAACATAGGCATCCACGGCAGCATCTCTGCCGAAGCCAAGGCCTGTGAGGAGAAGAAGAAGAAGCACAAGATGACGGCCTCCTTCCACCAAACCTACCGGGAGCGCCACTCGGAAGTGGTTGGCGGCCATCACACCTCCATTAACGACCTGCTGTTCGGGATCCAGGCCGGGCCCGAGCAGTACTCAGCCTGGGTAAACTCGCTGCCCGGCAGCCCTGGCCTGGTGGACTACACCCTGGAACCCCTGCACGTGCTGCTGGACAGCCAGGACCCGCGGCGGGAGGCACTGAGGAGGGCCCTGAGTCAGTACCTGACGGACAGGGCTCGCTGGAGGGACTGCAGCCGGCCGTGCCCACCAGGGCGGCAGAAGAGCCCCCGAGACCCATGCCAGTGTGTGTGCCATGGCTCAGCGGTCACCACCCAGGACTGCTGCCCTCGGCAGAGGGGCCTGGCCCAGCTGGAGGTGACCTTCATCCAAGCATGGGGCCTGTGGGGGGACTGGTTCACTGCCACGGATGCCTATGTGAAGCTCTTCTTTGGTGGCCAGGAGCTGAGGACGAGCACCGTGTGGGACAATAACAACCCCATCTGGTCAGTGCGGCTGGATTTTGGGGATGTGCTCCTGGCCACAGGGGGGCCCCTGAGGTTGCAGGTCTGGGATCAGGACTCTGGCAGGGACGATGACCTCCTTGGCACCTGTGATCAGGCTCCCAAGTCTGGTTCCCATGAGGTGAGATGCAACCTGAATCATGGCCACCTAAAATTCCGCTATCATGCCAGGTGCTTGCCCCACCTGGGAGGAGGCACCTGCCTGGACTATGTCCCCCAAATGCTTCTGGGGGAGCCTCCAGGAAACCGGAGTGGGGCCGTGTGGTGAGAACAGTGAGCTTGGAAAGGACCAGTATGCTTGGACTGAAGGGGTTCTCACAGTGGGAGCCAGGGCTGTCTTCGTATTCCCATTAGACCAAGCTTGTCCAACCCGAGGCCCGCATGCGGCCCAGGATGGCTTTGAATGCGGCCCAACGCAAATTCGCAAACTTTCTTAAAACATTATGAGTTTCTTTTTGCTATTTTTTTTTTTTTTTTAGCTCATCGGCTATCGTTAGTGCTAGTGGATTTTACATGTGGCCCAACACAATTCTTCTTCCAACGTGGCCCAGAGAAGCCAAAAGATTGGATACGCATCAGACAGATGGAAAAGGGAGATTCAGACTGTTTTTCAGGGAGGTGGCTGGGTTTACACGCTAATCCCGATTCACCCTGTCCAAACTGCCTAAGCCCTCCGCCATTCTCAAGCCCTGCAGTCACAGCTACACAGATCACAGCTTCAGCCAGGAGCTGGGCAGAAGGCCAAGAGGCTGTTCCCACCAGGCTGCTCAGGGCTGGTCTTTTAGGACCCTTCCCTTGAGCCCTCTATGGTGTGGCAAAGCCTTCATTGCCTTAACTGGAGCCCCATCAGCTCCAGCTGCTCTGTCTTCTTTGCCCACAATGCTTTGCCCCTGAGACAAATGGAGGCCTGTCCTGACCTGTCTCACCATGTACATAGCTTGATAAAGGGCCAATAAATATGATGTTATGGTGA NGG 10 8 17 8 24 0 100 5 10
    261021171559_fs1c AAGAGAAGCTTACTGGTACCATCCAAAATGATATACTAAAGGAATTTATGGTTCGAAATACATACATTTTTCCTCCAGAACCATCCATGAAAATTATTGCT(-TT)GACATATTTGAATATACAGCAAAGGTATACTTTGTGGTTATTATAGGTTTTTCTATAGTTCCTTGAATGTAGGAATTTTTACAACATGCTATAACTGAATT NG 50 8 18 8 68 0 100 5 30
    261020100817_o0jj CAGCTCTCTGCACGGAGTCACAGGGCGCGAGCCGGCGTTCGCCTTCTCCACGGAGCACGAGGAGGCGGCAAGCAA(/G)TGGCGGCAAGTTCGACCTGCCGGTGGACTCGGAGCACAAGGCGAAGAGCGTCCGTCTCTTCTCTGTGGCGAACCCGCACATGCGCACCTTCCACCTCTCCTGGATCTCCTTCTTCACCTGCTTCGTGTCCACCTTCGCCGCCGCGCCGCTGGTCCCCATCATCCGCGACAACCTCAACCTCACCA NGG 10 8 17 8 24 0 100 5 10
    261020100609_ei0d GCAGTCTCCGGGGTTGCGTGCAGGATGGTCGTCAGGGATCGGGAGTGAGGAGGCATCAACTCTCGCGGTCGTGGAGCCTAAA(/G)TGTACCGCAACAACGACTCGGCACTCTCCTGCTTCTACCTCTTCCTCCTCTGGTTCTTCTTCTTGAAGTAGACACCACCAGTTCGCCAGGTAGCTAGCAGCCCAGTTGCGACTGGGGGATCGGTGGCGGGCTGCCGTTTGCGAGTTGTAA NGG 10 8 17 8 24 0 100 5 10
    261019170221_pk22 CAGCTCTCTGCACGGAGTCACAGGGCGCGAGCCGGCGTTCGCCTTCTCCACGGAGCACGAGGAGGCGGCAAGCAA(/G)TGGCGGCAAGTTCGACCTGCCGGTGGACTCGGAGCACAAGGCGAAGAGCGTCCGTCTCTTCTCTGTGGCGAACCCGCACATGCGCACCTTCCACCTCTCCTGGATCTCCTTCTTCACCTGCTTCGTGTCCACCTTCGCCGCCGCGCCGCTGGTCCCCATCATCCGCGACAACCTCAACCTCACCA NGG 10 8 17 8 24 0 100 5 10
    261019165328_aunm GCAGTCTCCGGGGTTGCGTGCAGGATGGTCGTCAGGGATCGGGAGTGAGGAGGCATCAACTCTCGCGGTCGTGGAGCCTAAA(/G)TGTACCGCAACAACGACTCGGCACTCTCCTGCTTCTACCTCTTCCTCCTCTGGTTCTTCTTCTTGAAGTAGACACCACCAGTTCGCCAGGTAGCTAGCAGCCCAGTTGCGACTGGGGGATCGGTGGCGGGCTGCCGTTTGCGAGTTGTAA NGG 10 8 17 8 24 0 100 5 10
    261015085753_rmkx CAATGGCGGCCGTCGGCGCTCCGGGCAGCTCTCTGCACGGAGTCACAGGGCGCGAGCCGGCGTTCGCCTTCTCCACGGAGCACGAGGAGGCGGCAAGCAA(/G)TGGCGGCAAGTTCGACCTGCCGGTGGACTCGGAGCACAAGGCGAAGAGCGTCCGTCTCTTCTCTGTGGCGAACCCGCACATGCGCACCTTCCACCTCTC NGG 10 8 17 8 24 0 100 5 10
    261004083807_sj6q ATCTGATGGCTTTCTTTTCTCTCCCTCCCCCCTCCCTTGCTGTAGGTGGAGAATTCAGGGGACCCATGGGAAAATTTCCAAAACAACCAGGCACTTACGTACTGGGAATGTGTCTACTTACTCATGGTCACAATGTCTACAGTGGGTTATA(/G)GGGACGTTTATGCAAAAACCACACTTGGACGCCTCTTCATGGTCTTCTTCATCCTCGGGGGACTGGTAAAAAAAAGCCCTCTTTCTTATTGTATCTATTTTAATGATTATTAATATTTTCCAATGCCCACTTCCTTAGACATAGTATTTT NGG 10 8 17 8 24 0 100 5 10