Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    260718195053_lkfz CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    260717051551_656y tatgactgcacttgaaggaaaatgtacctttcaagttcagaaacacatgaacagttcctctctaactttctgtttttggtaataggacatctccaagtttgcagagaaggacaatatcgtccttggagaaggtggaatcacactgagtggTgA(/G)Tcagcgagcaagaatttccttagcaaggtgaatatcccattattggttcagcgagcatttgttgtaaatatcatgcatgtaaaaattatagacatttctctgttttttatattctgtttctgaaactgaaaaatgcctcgggttttatgat NG 10 8 17 8 24 0 100 5 200
    260717051526_pfkv tatgactgcacttgaaggaaaatgtacctttcaagttcagaaacacatgaacagttcctctctaactttctgtttttggtaataggacatctccaagtttgcagagaaggacaatatcgtccttggagaaggtggaatcacactgagtggTgA(/G)Tcagcgagcaagaatttccttagcaaggtgaatatcccattattggttcagcgagcatttgttgtaaatatcatgcatgtaaaaattatagacatttctctgttttttatattctgtttctgaaactgaaaaatgcctcgggttttatgat NGG 10 8 17 8 24 0 100 5 200
    260717050751_tpnh tatgactgcacttgaaggaaaatgtacctttcaagttcagaaacacatgaacagttcctctctaactttctgtttttggtaataggacatctccaagtttgcagagaaggacaatatcgtccttggagaaggtggaatcacactgagtggTgA(/G)Tcagcgagcaagaatttccttagcaaggtgaatatcccattattggttcagcgagcatttgttgtaaatatcatgcatgtaaaaattatagacatttctctgttttttatattctgtttctgaaactgaaaaatgcctcgggttttatgat NG 10 8 17 8 24 0 100 5 100
    260717050716_jij6 tatgactgcacttgaaggaaaatgtacctttcaagttcagaaacacatgaacagttcctctctaactttctgtttttggtaataggacatctccaagtttgcagagaaggacaatatcgtccttggagaaggtggaatcacactgagtggTgA(/T)Tcagcgagcaagaatttccttagcaaggtgaatatcccattattggttcagcgagcatttgttgtaaatatcatgcatgtaaaaattatagacatttctctgttttttatattctgtttctgaaactgaaaaatgcctcgggttttatgat NG 10 8 17 8 24 0 100 5 10
    260717050649_hadz tatgactgcacttgaaggaaaatgtacctttcaagttcagaaacacatgaacagttcctctctaactttctgtttttggtaataggacatctccaagtttgcagagaaggacaatatcgtccttggagaaggtggaatcacactgagtggTgA(/T)Tcagcgagcaagaatttccttagcaaggtgaatatcccattattggttcagcgagcatttgttgtaaatatcatgcatgtaaaaattatagacatttctctgttttttatattctgtttctgaaactgaaaaatgcctcgggttttatgat NGG 10 8 17 8 24 0 100 5 10
    260717050617_ojb3 tatgactgcacttgaaggaaaatgtacctttcaagttcagaaacacatgaacagttcctctctaactttctgtttttggtaataggacatctccaagtttgcagagaaggacaatatcgtccttggagaaggtggaatcacactgagtggTgA(/G)Tcagcgagcaagaatttccttagcaaggtgaatatcccattattggttcagcgagcatttgttgtaaatatcatgcatgtaaaaattatagacatttctctgttttttatattctgtttctgaaactgaaaaatgcctcgggttttatgat NG 10 8 17 8 24 0 100 5 100
    260717050525_1w05 tatgactgcacttgaaggaaaatgtacctttcaagttcagaaacacatgaacagttcctctctaactttctgtttttggtaataggacatctccaagtttgcagagaaggacaatatcgtccttggagaaggtggaatcacactgagtggTgA(/G)Tcagcgagcaagaatttccttagcaaggtgaatatcccattattggttcagcgagcatttgttgtaaatatcatgcatgtaaaaattatagacatttctctgttttttatattctgtttctgaaactgaaaaatgcctcgggttttatgat NGG 10 8 17 8 24 0 100 5 10
    260717035823_vjn2 AGCTGTGGACCTTCTGGGCCGGGACCGTGATGGGCTGGGTGAGGATGCCCa(/G)TGTGATGGCTGTGCTGCGTCACCTCCTCCTCAATGAGGACCCCCTCAACAG NGG 10 8 17 8 24 0 100 5 10
    260716033047_460p CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10