Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    261003123803_xlow ACTGGGAATGTGTCTACTTACTCATGGTCACAATGTCTACAGTGGGTTATA(/G)GGGACGTTTATGCAAAAACCACACTTGGACGCCTCTTCATGGTCTTCTTC NGG 10 8 17 8 24 0 100 5 10
    261003122933_x8w0 ACTGGGAATGTGTCTACTTACTCATGGTCACAATGTCTACAGTGGGTTATA(/G)GGGACGTTTATGCAAAAACCACACTTGGACGCCTCTTCATGGTCTTCTTC NGG 10 8 17 8 24 0 100 5 10
    260929173355_i23e CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    260929165837_09dw AGTTTCATCTGGAAAACCTGAGCC(/G)TGAGGATCCTGAAGAGCAGGAAG NGG 10 8 17 8 24 0 100 5 10
    260929104352_fzit AGTTTCATCTGGAAAACCTGAGCC(/G)TGAGGATCCTGAAGAGCAGGAAG NGG 10 8 17 8 24 0 100 5 10
    260929104153_wr6v GTTTCATCTGGAAAACCTGAGCCT(/A)GAGGATCCTGAAGAGCAGGAAGG NGG 10 8 17 8 24 0 100 5 10
    260929104141_ckdz AGTTTCATCTGGAAAACCTGAGCC(/G)TGAGGATCCTGAAGAGCAGGAAG NGG 10 8 17 8 24 0 100 5 10
    260929104130_ro2a AGTTTCATCTGGAAAACCTGAGCC(/G)TGAGGATCCTGAAGAGCAGGAAG NGG 10 8 17 8 24 0 100 5 10
    260929103304_yfh8 TTTCATCTGGAAAACCTGAGCCTG(/C)AGGATCCTGAAGAGCAGGAAGGA NGG 10 8 17 8 24 0 100 5 10
    260929103136_h4ds GTTTCATCTGGAAAACCTGAGCCT(/A)GAGGATCCTGAAGAGCAGGAAGG NGG 10 8 17 8 24 0 100 5 10