Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250724130517_ytae CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250724125147_3dhn CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250724124800_ukgg tgtttttggagtggtggagcctccgaggcgctctaggggaaggttgattatgcacgggtcggcc(-ggaagg)atgtttggaagggaattctgctacgctg NGG 10 8 17 8 24 0 100 5 10
    250724123749_sbg7 tgtttttggagtggtggagcctccgaggcgctctaggggaaggttgattatgcacgggtcggcc(-GCTGGCGCGA)atgtttggaagggaattctgctacgctg NGG 10 8 17 8 24 0 100 5 10
    250724084703_m8i9 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250724044458_odwc CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250724043954_durm GACTTCGTTCATACTCCCTCCGTCCGAAAAAGATTGTCCCTCAAATGGATGTATCTAGAACTAACTTGGTGCTAGATACATC(+ATT)CATTTGATGGACAAGCTTGGGACAAGCTTTTTTGGATGGAGGAAGTACGATTCTAATGAGCCCAAGAACATCAAAATCCGAGTTCGGAATGACAATCATTCTCCGAAGAAGACCAGAACGTTCGATGAGGCACGGTACAACTATTCCATGCCCAGAAAAGCCCAAAATGGTTCAGACCAGATGGGATTCTTGGTGGATTTCATTCCTATTTTTTTCCTTCGACATCTTTGGCCATCAAAGGAAGTTTTGGCAGAGGATTTGGCTCATTTAGAGCCCTTGAACCAAAGGAGAAAAGATACATCTACGGATGAGGCTTTCATAGAGCCCTTATATACACATCTTCAAGCCTAGTCGCCTCCATCATCCATCATACATCCAAGATCAACCTCCATCACGTCTATTGCTACTCCAACACCACCTCGATAGAAGAAGGAACGAGATCAAGAGAATGAAAAGAAGGCTCTAGCACACACCGCATCGGCCTTCGGGGCGGTGCTTTCTCCACCATGCCACCGCTGACATCACTATCTCTACCACGCCATCACCATTGGTGCCGCCTCCACCAACGCCACCATGTGTTCTCCATTCCCCTACTCGATGCCGACTTGTAACTTGACTCTCTCCTCTATGTTACCCGACTTTATGTTTGAGCAGTTGTACTTGTTCTTGGGTATATGGATGAACTCTTTATGATTAGAATTAAATTCAATTAGGCGAGCCAATATCTTTTGTTTATGTTGGAGTAGCTTTCATGTGTGTTGTGAGGAGTACGCACACAACATTGTCGCCTTGATTGGCTGTCAACCATATTACTGCTGTTGTTCTGGGTTAGGATGTTGAAGTAATTTGAGGTGCCGGTAAAAAGCCTCTACCTTCACCTTGTCCAATCCGTAGGGGAATAATAGAGAACCCTTGGTGAGGCCGCGTCTGTATGCGAAACCACCCACTTAAGCACATAAAGAGTAGTTTAGGGACAATTCATAGACCTATGCTCCGCACTTGTTGCATGGATCATATTAGTAGTGATGACTACGGAACACTCGAGTGACCTTATTGCTCAAGCACTGCCTATACTAACTTAGCATTTTTCTTATTCCTATTTTTACCTTCAGTTCGTATTCTAGGTAAAGTCATTTTTATTCCGTTTCTGCAGCGATTTATAGAATAGGCTATTTCCAAACTCCGGTTTGGGCGTGTACCGAATTTCATCGGTGAAAATGTAGTTACAGGGTTAGTAGTGCCTTACTTTTCTCTCCTTGCGGGTTCGACACTCTATTTGCCATGGAAATGCTACAACACCCTATGCTCTTGCTGTACATCAAGTCCTTTCGATACTGATGTATAACTTTTGATATTATAAACACCGGTTTCGAGGATCTTCGTGTCCCTTGTCAAAAAAAAATACATGGATTTGTACAAACTATAGAGTACGCATGTTGGCACGGCAAACTATTGTCCCACCTCACCAACCAAGAGCTAAGATGGAATTGGAGAACATACTTCTAGTTGATATCCATACAAGTGTAGAAGTATCTATTTGTTTAACAAGCAGGAGCTAGGATGGGATTGGTCTAGAAGTATCTATTTACAAATGGAGTACTCTCAAAACATTTGGACATTTATCTATAAGATGCATACATTACTCTTAAATACAGTAGCAAGTTTAATCGACATACGGTGACCTGCAAACAACATAATGTGGTAGTATTGGTTTATTGAGATTTACAAAGATATCCATTGTAACAACATAGTGGCTTAAACGTCCGAAACAAAATAAAAAACATTCTTTGTGACAAAATACAATTTATTTTTAGATCTGTAAAATATGTTTCTACTGATAAACATTAGAAGAGTATGAAAAATAAGTACCTAAGATGCAGGAGCTTGTGTAAATTGTTCATTTTTCTTATTATTTATACAATAATATTTAGGCTGGTATGTTGAATAAATAATATCAATAGAAACAATATATATCTTTACATCAAGATGAGTTATCACGAAGTAAAAAAGTTCAATATAAATAGGTGGTACTCCCTTTATTCTGGTAGATAAGCCCAGTTTTTACCGATGCATTAGCTGGAAATAATGACATCCGTTAAATCGGTTTTATTAATTGTGCTTATCTATTAATTTTCCTAAAATTAACTATTTTACATAAATATTGTGACCAACAAAACATTCAAGCGTCACATCTTGATTTGACATCTCCGGTATGTATATACTTTAT NGG 10 8 17 8 24 0 100 5 10
    250723170533_22md GGCTTCATCACCCCCGACGACGGCAGCAAGGACGTCTTCGTCCACTTCAGCGCAATCCATGGCAAGGGCTTCAAGACCCTCGCCGAGGGCCAGCGTGTCGAATTCGAAATTCAAGACGGCCAAAAAGGCCCGTCCGCCGTCAATGTCAAGGCCCTAGAGCTAGAGGAGGATAAAAGG(+A)ATTTCGAGCAAATTGTAAGAGGATGCTCCACCCATCCACATCGTGCGATGCGTGATATCCTATGCTGCGTTCGGAAGGATTTGCTATTGTTTGTATTCTCTTCTCACCTGTGCACTAAAATCTGTACCGTTTCTCCTTTTCTTTTTTACATCA NGG 10 8 17 8 24 0 100 5 10
    250723155432_8vz2 ggtaggacacgcaggcctccg(-cca)gggcagcacagacactgtgagagatgcttcaaccgtcactgccacatgcctgtggagcccagtgtctcttgcccggtgataaactgccacctgtcc NGG 10 8 17 8 24 0 100 5 10
    250723154847_0zsg CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 15 10 14 0 100 5 10