Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    260914210122_tmc4e8xh GACATATGCATTAACACTCTGGTGTTTTGTTTCCCTCTGTAGAATCTACACCCCATTCACAGAAGACACTGAGACTGTAGGCCAAAGAGCCCTGCACTCGATCCTGAATGCGGCCATCATGATCAGTGTCATTGTCATTA(/C)TGACCATCCTCCTGGTGGTCCTGTATAAATACAGGTGCTACAAGGTGAGCACGAGGCAGTCTGCTGTCCGCCTTGACCTGCCTATGGGTTGTCCTTGTGTGTGTGGGAGGGGAAGGGTGGGAGAGTTGGGGGCGGGGGAGATGTGTATGCAGGCCTGTGC NGG 10 8 17 8 24 0 100 5 10
    260914210122_tmc4d4ai GCTACATCCGTTGCCTCTTGCGCTTACAGACCTTTTTCTCGGCTTCCTTTCGAAGGTGTTCTTTGCTGAAGATGTGGGTTCGAACAAAGGCGCCATCATCGGACTCATGGTGGGCGGCGTTGTCATAGCAACCGTGA(/G)TTGTCATCACCCTGGTGATGTTGAAGAAGAAACAGTACACATCCATCCATCATGGCGTGGTGGAGGTAGGTAAACCTGGAGGCTTGTCTGCAAGTGGGAGGCAAGCCAGGAAGTACAGGCACTGGCCCGGGGCCACTTAGGAGAAAAGCAACATCCCTCTGTG NGG 10 8 17 8 24 0 100 5 10
    260914210122_tmc49orj CATTCAGACCATAGCACTTCCCTTCCAAATACTAAAATTGTTTTTCTCTCTTCTTTATTTCCAGCAATGTCTCAGGCTGTGCAGACAAACGGAACTCAACCATTAAGCAAAACATGGGAACTCAGTTTATATGAGTTACAACGAACACCTCAGGTA(+TGT)ATGACTAAGATGACTGCCAAGGGGCATATGAGACGTGTAAACTGGGATACATTTTTAGAACATGAAAACTTAAATAGAACATAGAGTAATAATAAATTTATATTCAAGCATTCCTGACTTCTGTATGTTGGCTAACACTTTATT NGG 10 8 17 8 24 0 100 5 10
    260914210122_tmc472y4 AGATTCTTACGTATCTCTTCGAACTCCAACCTGTCACCGTTTTAGATCCAAACCCACCAATCACGAACCTAAGGATGAAAGCAAAGGCTCAGCAGTTGACATGGGACCTTAACAGAAATGTGACCGATATCGAGTGTGTTAAAGACGC(/T)CGACTATTCTATGCCGGTAAATCATACTCTCTATTGTTTTTTTATTTTTATTTTATTTATTTATGTATTTATGTATTTATTTATTTTTTGAGACGGAGTCTTGCTCTGTCGCCCAGGCTGGACTGCGGTGACCCGATCTCCGCTCTCTGCAA NGG 10 8 17 8 24 0 100 5 10
    260914210122_tmc46h4u GCCCCAGTGGCTGCTCTGGGGGCCTCCTGAGTTTCTCATCTGTGCCCCTCCCTCCCTGGCCCAGGTGAAGGTGTGGTTCCAGAACCGGAGGACAAAGTACAAACGGCAGAAGCTGGAGGAGGAAGGGCCTGAGTCCGAGCAGAAGAAGAA(-GGG)CTCCCATCACATCAACCGGTGGCGCATTGCCACGAAGCAGGCCAATGGGGAGGACATCGATGTCACCTCCAATGACTAGGGTGGGCAACCACAAACCCACGAGGGCAGAGTGCTGCTTGCTGCTGGCCAGGCCCCTGCGTGGGCCCA NGG 10 8 17 8 24 0 100 5 10
    260914210122_tmc43xqr CAGCCGCGGTGGGAGGAGGCACCGGCCGCGCGGCGGGAGGAGGTGCCGGCCGAGCACCGCAGACAGCGCCGGGAAGATGAGCACGGAAGGTGGTGGCCGTCGCTGCCAGGCACAAGTGTCCCGCCGCATCTCCTTCAGCGCGAGCCACC(/G)GATTGTACAGGTAGGGTGTGCACACAGGTACAGCGGCGGGCGGTGGGCGCCGGGCCCCGGAACGTCACCGGGGCGGGGCCGGCGGGCGTGCTGACGTCGGGCCCGGGAGGGGCGCGGGGGCTGCTGGGGCGACGCGCGCTGGTCGGCTTCG NGG 10 8 17 8 24 0 100 5 10
    260914150432_992p CTAGGGTCGTGCCGCCCCTGCAGTCTCCGGGGTTGCGTGCAGGATGGTCGTCAGGGATCGGGAGTGAGGAGGCATCAGCTCTCGCGGTCGTGGAGCCTAAA(/G)TGTACCGCAACAACGACTCGGCACTCTCCTGCTTCTACCTCTTCCTCCTCTGGTTCTTCTTCTTGAAGTAGACACCACCAGTTCGCCAGGTAGTTAGCAG NGG 10 8 17 8 24 0 100 5 10
    260914150426_n8kj CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    260914150423_l6l1 CTAGGGTCGTGCCGCCCCTGCAGTCTCCGGGGTTGCGTGCAGGATGGTCGTCAGGGATCGGGAGTGAGGAGGCATCAGCTCTCGCGGTCGTGGAGCCTAAA(/G)TGTACCGCAACAACGACTCGGCACTCTCCTGCTTCTACCTCTTCCTCCTCTGGTTCTTCTTCTTGAAGTAGACACCACCAGTTCGCCAGGTAGTTAGCAG NGG 10 8 17 8 24 0 100 5 10
    260914143536_21r6 CTAGGGTCGTGCCGCCCCTGCAGTCTCCGGGGTTGCGTGCAGGATGGTCGTCAGGGATCGGGAGTGAGGAGGCATCAGCTCTCGCGGTCGTGGAGCCTAAA(/G)TGTACCGCAACAACGACTCGGCACTCTCCTGCTTCTACCTCTTCCTCCTCTGGTTCTTCTTCTTGAAGTAGACACCACCAGTTCGCCAGGTAGTTAGCAG NGG 10 8 17 8 24 0 100 5 10