Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    260531213921_d3jk ATTAATTAGGGTGGCTTGATCAGCTACAGAAG(+CGACTGAGATCAC)GATCCTTTGGGGTCACATTCTTTTATCCTGT NGG 10 8 17 8 24 0 100 5 10
    260527231941_k07w CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    260527035927_3afk CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    260526103628_oxc1 TGAGTCAGAACATGATCTGTTGCTTAATGGGTGCTTGAAATCTCTGGCTTTTGACTCTGAACATTCTGCAGATGAAAAGGAAAAGCCTTGTGTTAAATCTC(/T)GAGCCAGAAAGAGTTCTGATAATCCAAAAAGGACTAGTGTGAAAAAGGGCCACATGCAATTTGAAGCACATAAGGAAGAACGGAGGGGAAAGATTCCAGA NGG 10 8 15 8 24 0 100 5 10
    260526102824_2ryv TGAGTCAGAACATGATCTGTTGCTTAATGGGTGCTTGAAATCTCTGGCTTTTGACTCTGAACATTCTGCAGATGAAAAGGAAAAGCCTTGTGTTAAATCTC(/T)GAGCCAGAAAGAGTTCTGATAATCCAAAAAGGACTAGTGTGAAAAAGGGCCACATGCAATTTGAAGCACATAAGGAAGAACGGAGGGGAAAGATTCCAGA NGG 10 8 17 8 24 0 100 5 10
    260522211142_4nic GTTTTGGTCCAATATCGTGAACTTCAGGTAGACACAAGACATCTCTCTGTAGTTATTCTAGAGAAAATGCAGTTTATAATTTACCAGGCAGTAGTAAACATTCTAGAATCAGTACTAAGTTTCTCTTTATGGAAGTAAACATGCCATCTTGATACACTTATTATTCGAAGAAGCAAAAGTAGATACGTTCTTACAGAAGGCAAAAAAAAAAAAATTTTGCTAAGTGAAGTAAAAAATGTACTACTCTCTGCTTCTTCCCACAGTATCCATAAATATGCTGAGGCCGTTTATTTTGCCAGATGGGTTTTGAGACCCTGCTGAAACAAGAGATGCTCTCATTTATATTTCCCTCAAATTCTACCACAAACCACACTCGGGAGGGAAAAGAAAAAGTCGCCACGTAAGCATGTTTACCTTCAAGTGACTGGGAAGTGGAAACCTATCCATAAGTGATGAC(/A)TCACCATTGCAGGCCTATAAAAGTAAAGGTAATCTCTGCGGAAAGACAACAGTCCCCAGGCATCACCATTCAAGATGCATCCAGGGGTCCTGGCTGCCTTCCTCTTCTTGAGCTGGACTCATTGTCGGGCCCTGCCCCTTCCCAGTGGTGGTGATGAAGATGATTTGTCTGAGGAAGACCTCCAGTTTGCAGAGGTAGAGTATCTTGCCAATCCTGATGATGCGGTTGGTACATCTCAGAAATGTCCTTCTCTTTTAAACGCAGTTCATTTTGGTGTCTTTTCTAGCGCTACCTGAGATCATACTACCATCCTACAAATCTCGCGGGAATCCTGAAGGAGAAT NGG 10 8 17 8 24 0 100 5 10
    260522210833_x7q3 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    260522162540_rkbv AGAACAAGTGGGGTTGGTTGGTTTTTAGTTTAAGACTAGGGTTTCTCAGTATAGCCTTGGCTACCCTCAGACTCCCCAG(+ctt)TGCAGGGATTAAAGgcgcgCTACCACCAGCCCAGAACACATGTTGGCCTAATTGCTGAACCATCTCTCCAGCTTCCCAGTCCCCATTTTGATATTTTTATTCTACACATAGGAGAATACCTGCCTCTATCTCTGGAATGCTGGGATTATAGACACATGCCACCAAACCTAGCCTTATAACTTAATTTTTTAGAAAGATTTTTGCCAGTAGGGGTAGCACACAAATTTACCAG NGG 10 8 17 8 24 0 100 5 40
    260522162308_0e7s AGAACAAGTGGGGTTGGTTGGTTTTTAGTTTAAGACTAGGGTTTCTCAGTATAGCCTTGGCTACCCTCAGACTCCCCAG(+ctt)TGCAGGGATTAAAGCcggcCTACCACCAGCCCAGAACACATGTTGGCCTAATTGCTGAACCATCTCTCCAGCTTCCCAGTCCCCATTTTGATATTTTTATTCTACACATAGGAGAATACCTGCCTCTATCTCTGGAATGCTGGGATTATAGACACATGCCACCAAACCTAGCCTTATAACTTAATTTTTTAGAAAGATTTTTGCCAGTAGGGGTAGCACACAAATTTACCAG NGG 10 8 17 10 40 0 100 5 30
    260522162122_w1en AGAACAAGTGGGGTTGGTTGGTTTTTAGTTTAAGACTAGGGTTTCTCAGTATAGCCTTGGCTACCCTCAGACTCCCCAG(+ctt)TGCAGGGATTAggggggggCTACCACCAGCCCAGAACACATGTTGGCCTAATTGCTGAACCATCTCTCCAGCTTCCCAGTCCCCATTTTGATATTTTTATTCTACACATAGGAGAATACCTGCCTCTATCTCTGGAATGCTGGGATTATAGACACATGCCACCAAACCTAGCCTTATAACTTAATTTTTTAGAAAGATTTTTGCCAGTAGGGGTAGCACACAAATTTACCAG NGG 10 8 17 8 24 0 100 5 10