Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    260709175108_y6wf CCAAGGACAAGTCGATGGCGGCGGCGGCGGGGGCAGGGGAGCAGGAGGAGGAGGTGGACGAGCTGCTGGCGGCGCTCG(/A)GGTACAAGGTGCGTTCGTCGGATATGGCGGACGTCGCGCAGAAGCTAGAGCAGCTCGAGATGGCCATGGGGATGGGCGGCGCCTGCCCCACCGCTGATGACGGGTTCGTCTCGCACCTCGCCACGGACACCGTGCACTACAATCCCTCCGACCTGTCGTCCTGGGTCGAGAGCATGCTGTCCGAGCTCAACGCGCCCCCGCCGC NGG 10 8 17 8 24 0 100 5 10
    260709175057_t01s CCAAGGACAAGTCGATGGCGGCGGCGGCGGGGGCAGGGGAGCAGGAGGAGGAGGTGGACGAGCTGCTGGCGGCGCTCG(/A)GGTACAAGGTGCGTTCGTCGGATATGGCGGACGTCGCGCAGAAGCTAGAGCAGCTCGAGATGGCCATGGGGATGGGCGGCGCCTGCCCCACCGCTGATGACGGGTTCGTCTCGCACCTCGCCACGGACACCGTGCACTACAATCCCTCCGACCTGTCGTCCTGGGTCGAGAGCATGCTGTCCGAGCTCAACGCGCCCCCGCCGC NGG 10 8 17 8 24 0 100 5 10
    260709174839_q3qm CTTTCAATGTTCCTGCCATGTATGTTGCAATCCAAGCAGTTCTATCACTCTATGCTAGTGGGCGTACTACTGGTATTGTTCTTGACTCTGGTG(/A)ATGGTGTGAGCCACACTGTGCCCATTTACGAAGGATATGCCCTTCCTCATGCC NGG 10 8 17 8 24 0 100 5 10
    260709172839_mcnm AACTTAATTGTTTACATGGTGTGGCTGGTGTTTCCTCTTTTTTTTTCTCAATCTTCAGGTATTGTTCTTGACTCTGGTG(/A)ATGGTGTGAGCCACACTGTGCCCATTTACGAAG NGG 10 8 17 8 24 0 100 5 10
    260709165642_xo7o CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    260709143738_2sgv CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    260709112905_ywk4 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    260708034848_0vg6 acattttaaaaattacagaaaaatttacatctaaaatttcagcaatgttgtttttgaccaactaaataaattgcatttgaaataatggagatgcaatgttcaaaatttcaactgtggttaaagcaatagtgtgatatatgattacattagaaggaagatgtgcctttcaaattcagattgagcatactaaaagtgactctctaattttctatttttggtaatagGACATCTCCAAGTTTGCAGAGAAAGACAATATAGTTCTTGGAGAAGGTGGAATCACACTGAGTGGAGA(/T)TCAACGAGCAAGAATTTCTTTAGCAAGgtgaataactaattattggtctagcaagcatttgctgtaaatgtcattcatgtaaaaaaattacagacatttctctattgctttatattctgtttctggaattgaaaaaatcctggggttttatggctagtgggttaagaatcacatttaagaactataaataatggtatagtatccagatttggtagagattatggttactcagaatctgtgcccgtatcttggtgtcagtgtatttgtttgcctcatagtatagtttactacaaatggaaaactctaggattc NGG 10 8 17 8 24 0 200 5 50
    260708034826_4ul6 acattttaaaaattacagaaaaatttacatctaaaatttcagcaatgttgtttttgaccaactaaataaattgcatttgaaataatggagatgcaatgttcaaaatttcaactgtggttaaagcaatagtgtgatatatgattacattagaaggaagatgtgcctttcaaattcagattgagcatactaaaagtgactctctaattttctatttttggtaatagGACATCTCCAAGTTTGCAGAGAAAGACAATATAGTTCTTGGAGAAGGTGGAATCACACTGAGTGGAGA(/T)TCAACGAGCAAGAATTTCTTTAGCAAGgtgaataactaattattggtctagcaagcatttgctgtaaatgtcattcatgtaaaaaaattacagacatttctctattgctttatattctgtttctggaattgaaaaaatcctggggttttatggctagtgggttaagaatcacatttaagaactataaataatggtatagtatccagatttggtagagattatggttactcagaatctgtgcccgtatcttggtgtcagtgtatttgtttgcctcatagtatagtttactacaaatggaaaactctaggattc NGG 10 8 17 8 24 0 200 5 10
    260708034639_gxp7 attacattagaaggaagatgtgcctttcaaattcagattgagcatactaaaagtgactctctaattttctatttttggtaatagGACATCTCCAAGTTTGCAGAGAAAGACAATATAGTTCTTGGAGAAGGTGGAATCACACTGAGTGGAGA(/T)TCAACGAGCAAGAATTTCTTTAGCAAGgtgaataactaattattggtctagcaagcatttgctgtaaatgtcattcatgtaaaaaaattacagacatttctctattgctttatattctgtttctggaattgaaaaaatcctggggtttta NG 10 8 17 8 24 1 95 5 10