Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    260708034005_phsc gactgcacttgaaggaaaatgtacctttcaagttcagaaacacatgaacagttcctctctaactttctgtttttggtaataggacatctccaagtttgcagagaaggacaatatcgtccttggagaaggtggaatcacactgagtggTgA(/T)Tcagcgagcaagaatttccttagcaaggtgaatatcccattattggttcagcgagcatttgttgtaaatatcatgcatgtaaaaattatagacatttctctgttttttatattctgtttctgaaactgaaaaatgcctcgggttttatga NGG 10 8 17 8 24 0 100 5 50
    260708033907_c8gq gactgcacttgaaggaaaatgtacctttcaagttcagaaacacatgaacagttcctctctaactttctgtttttggtaataggacatctccaagtttgcagagaaggacaatatcgtccttggagaaggtggaatcacactgagtggTgA(/T)Tcagcgagcaagaatttccttagcaaggtgaatatcccattattggttcagcgagcatttgttgtaaatatcatgcatgtaaaaattatagacatttctctgttttttatattctgtttctgaaactgaaaaatgcctcgggttttatga NG 10 8 17 8 24 0 100 5 50
    260708033827_oqrn gactgcacttgaaggaaaatgtacctttcaagttcagaaacacatgaacagttcctctctaactttctgtttttggtaataggacatctccaagtttgcagagaaggacaatatcgtccttggagaaggtggaatcacactgagtggTgA(/T)Tcagcgagcaagaatttccttagcaaggtgaatatcccattattggttcagcgagcatttgttgtaaatatcatgcatgtaaaaattatagacatttctctgttttttatattctgtttctgaaactgaaaaatgcctcgggttttatga NGG 10 8 17 8 24 0 100 5 10
    260708033607_rzxl attacattagaaggaagatgtgcctttcaaattcagattgagcatactaaaagtgactctctaattttctatttttggtaatagGACATCTCCAAGTTTGCAGAGAAAGACAATATAGTTCTTGGAGAAGGTGGAATCACACTGAGTGGAGA(/T)TCAACGAGCAAGAATTTCTTTAGCAAGgtgaataactaattattggtctagcaagcatttgctgtaaatgtcattcatgtaaaaaaattacagacatttctctattgctttatattctgtttctggaattgaaaaaatcctggggttt NG 10 8 17 8 24 0 100 5 50
    260708033522_escu attacattagaaggaagatgtgcctttcaaattcagattgagcatactaaaagtgactctctaattttctatttttggtaatagGACATCTCCAAGTTTGCAGAGAAAGACAATATAGTTCTTGGAGAAGGTGGAATCACACTGAGTGGAGA(/T)TCAACGAGCAAGAATTTCTTTAGCAAGgtgaataactaattattggtctagcaagcatttgctgtaaatgtcattcatgtaaaaaaattacagacatttctctattgctttatattctgtttctggaattgaaaaaatcctggggttt NG 10 8 17 8 24 0 100 5 10
    260708023659_xtqq CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    260708023218_syyp GTCCTCTACTCCTTTCTGATTAAATTTGGTCATGTACTGATGCATGTAGGG(/A)ATAGTTATTCCGAATGTTTCTCTCCGTGCTTCCATTAGGCACTGTCCCA NGG 10 12 14 10 20 0 90 5 10
    260708023202_zaua GTCCTCTACTCCTTTCTGATTAAATTTGGTCATGTACTGATGCATGTAGGG(/T)ATAGTTATTCCGAATGTTTCTCTCCGTGCTTCCATTAGGCACTGTCCCA NGG 10 8 17 8 24 0 100 5 10
    260708022854_vflj GTCCTCTACTCCTTTCTGATTAAATTTGGTCATGTACTGATGCATGTAGGG(/A)ATAGTTATTCCGAATGTTTCTCTCCGTGCTTCCATTAGGCACTGTCCCA NGG 10 8 17 8 24 0 100 5 10
    260707234515_t3a9 TGGTGAAAAGGAAACTGCCATCCTTACCCCGTTTTCAGGCTGACCAAGGCGGG(/A)TCCTCTACTCCTTTCTGATTAAATTTGGTCATGTACTGATGCATGTAGGGATAGTTATTCCGAATGTTTCTCTCCGTGCTTCCATTAGGCACTGTCCCAAATCGAAAAGGTGGGGAATAGTCATGAGGTCTCTGAAACTGGAGAGAGAAC NGG 10 8 17 8 24 0 100 5 10