Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    260113152954_ozfu CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    260113035443_7vpd GGGGCGGCTGGGCAACGACCCCACGCGGCGACGGCCCCCCCCCGCCCCGCAGATGAGGAGCAGCTC(/G)TAGGCCGAAGTGTCGCAGGCCGGGACCGTCCTGGA NGG 10 8 17 8 24 0 100 5 10
    260113035359_3007 GGGGGGCGGCTGGGCAACGACCCCACGCGGCGACGGCCCCCCCCCGCCCCGCAGATGAGGAGCAGCTC(/G)TAGGCCGAAGTGTCGCAGGCCGGGACCGTCCTG NGG 10 8 17 8 24 0 100 5 10
    260113035315_640q GGGGCGGCTGGGCAACGACCCCACGCGGCGACGGCCCCCCCCCGCCCCGCAGATGAGGAGCAGCTCTA(/G)GGCCGAAGTGTCGCAGGCCGGGACCGTCCTGGA NGG 10 8 17 8 24 0 100 5 10
    260113035249_xcxr GGGGGGCGGCTGGGCAACGACCCCACGCGGCGACGGCCCCCCCCCGCCCCGCAGATGAGGAGCAGCTC(/G)TAGGCCGAAGTGTCGCAGGCCGGGACCGTCCTG NGG 10 8 17 8 24 0 100 5 10
    260113035050_5c0l GGGGGGCGGCTGGGCAACGACCCCACGCGGCGACGGCCCCCCCCCGCCCCGCAGATGAGGAGCAGCTC(/G)TAGGCCGAAGTGTCGCAGGCCGGGACCGTCCTG NGG 10 8 17 8 24 0 100 5 10
    260113034018_njqd GGGGGCGGCTGGGCAACGACCCCACGCGGCGACGGCCCCCCCCCGCCCCGCAGATGAGGAGCAGCTC(/G)TAGGCCGAAGTGTCGCAGGCCGGGACCGTCCTGGA NGG 10 8 17 8 24 0 100 5 10
    260113031346_y4ci GGGGGCGGCTGGGCAACGACCCCACGCGGCGACGGCCCCCCCCCGCCCCGCAGATGAGGAGCAGCTCTA(/G)GGCCGAAGTGTCGCAGGCCGGGACCGTCCTGGA NGG 10 8 17 8 24 0 100 5 10
    260112221308_sqdg AGACTGGTCGTTCTCGAGGATTTTGTTTCGTGTACTTCGAGAACCAGGAGGATGCGAAAGTTGCAAAGAACGAGTGTACAGGCATGGAGATAGATGGACGTCGCATCCGCGTCGACTACTCGATCACGCACCGTGCGCATACC(/t)CGACACCCGGTATCTACATGGGGAAACCTACG NGG 10 8 17 8 24 0 100 5 10
    260112220621_agjz AGACTGGTCGTTCTCGAGGATTTTGTTTCGTGTACTTCGAGAACCAGGAGGATGCGAAAGTTGCAAAGAACGAGTGTACAGGCATGGAGATAGATGGACGTCGCATCCGCGTCGACTACTCGATCACGCACCGTGCGCATACC(/T)CGACACCCGGTATCTACATGGGGAAACCTACG NGG 10 8 17 8 24 0 100 5 10