Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    260522162057_cs51 AGAACAAGTGGGGTTGGTTGGTTTTTAGTTTAAGACTAGGGTTTCTCAGTATAGCCTTGGCTACCCTCAGACTCCCCAG(+ctt)TGCAGGGATTAAAggggggCTACCACCAGCCCAGAACACATGTTGGCCTAATTGCTGAACCATCTCTCCAGCTTCCCAGTCCCCATTTTGATATTTTTATTCTACACATAGGAGAATACCTGCCTCTATCTCTGGAATGCTGGGATTATAGACACATGCCACCAAACCTAGCCTTATAACTTAATTTTTTAGAAAGATTTTTGCCAGTAGGGGTAGCACACAAATTTACCAG NGG 10 8 17 8 24 0 100 5 10
    260522161807_12n3 AGAACAAGTGGGGTTGGTTGGTTTTTAGTTTAAGACTAGGGTTTCTCAGTATAGCCTTGGCTACCCTCAGACTCCCCAG(+ctt)TGCAGGGATTAAAGCAgggCTACCACCAGCCCAGAACACATGTTGGCCTAATTGCTGAACCATCTCTCCAGCTTCCCAGTCCCCATTTTGATATTTTTATTCTACACATAGGAGAATACCTGCCTCTATCTCTGGAATGCTGGGATTATAGACACATGCCACCAAACCTAGCCTTATAACTTAATTTTTTAGAAAGATTTTTGCCAGTAGGGGTAGCACACAAATTTACCAG NGG 10 8 17 8 24 0 100 5 10
    260522161625_7eoz TTGGCTACCCTCAGACTCCCCAG(+ctt)TGCAGGGATTAAAGCACCACTACCACCAGCCCAGAACACATGTTGGCCTAATTGCTGAACCATCTCTCCAGCTTCCCAGTCCCCATTTTGATATTTTTATTCTACACATAGGAGAATACCTGCCTCTATCTCTGGAATGCTGGGATTATAGACACATGCCACCAAACCTAGCCTTATAACTTAATTTTTTAGAAAGATTTTTGCCAGTAGGGGTAGCACACAAATTTACCAG NGG 10 8 17 8 24 0 100 5 10
    260522155915_mywe TTCTCTGACAGAACAAGTGGGGTTGGTTGGTTTTTAGTTTAAGACTAGGGTTTCTCAGTATAGCCTTGGCTACCCTCAGACTCCCCAGTGC(+ctt)AGGGATTAAAGCACCACTACCACCAGCCCAGAACACATGTTGGCCTAATTGCTGAACCATCTCTCCAGCTTCCCAGTCCCCATTTTGATATTTTTATTCTACACATAGGAGAATACCTGCCTCTATCTCTGGAATGCTGGGATTATAGACACATGCCACCAAACCTAGCCTTATAACTTAATTTTTTAGAAAGATTTTTGCCAGTAGGGGTAGCACACAAATTTACCAG NGG 10 8 17 8 24 0 100 5 10
    260522155827_bpoc TTCTCTGACAGAACAAGTGGGGTTGGTTGGTTTTTAGTTTAAGACTAGGGTTTCTCAGTATAGCCTTGGCTACCCTCAGACTCCCCAGTG(+ctt)CAGGGATTAAAGCACCACTACCACCAGCCCAGAACACATGTTGGCCTAATTGCTGAACCATCTCTCCAGCTTCCCAGTCCCCATTTTGATATTTTTATTCTACACATAGGAGAATACCTGCCTCTATCTCTGGAATGCTGGGATTATAGACACATGCCACCAAACCTAGCCTTATAACTTAATTTTTTAGAAAGATTTTTGCCAGTAGGGGTAGCACACAAATTTACCAG NGG 10 8 17 8 24 0 100 5 10
    260522155807_buel TTCTCTGACAGAACAAGTGGGGTTGGTTGGTTTTTAGTTTAAGACTAGGGTTTCTCAGTATAGCCTTGGCTACCCTCAGACTCCCCAG(+GT)TGCAGGGATTAAAGCACCACTACCACCAGCCCAGAACACATGTTGGCCTAATTGCTGAACCATCTCTCCAGCTTCCCAGTCCCCATTTTGATATTTTTATTCTACACATAGGAGAATACCTGCCTCTATCTCTGGAATGCTGGGATTATAGACACATGCCACCAAACCTAGCCTTATAACTTAATTTTTTAGAAAGATTTTTGCCAGTAGGGGTAGCACACAAATTTACCAG NGG 10 8 17 8 24 0 100 5 10
    260522155749_hlcj TTCTCTGACAGAACAAGTGGGGTTGGTTGGTTTTTAGTTTAAGACTAGGGTTTCTCAGTATAGCCTTGGCTACCCTCAGACTCCCCAG(+actg)TGCAGGGATTAAAGCACCACTACCACCAGCCCAGAACACATGTTGGCCTAATTGCTGAACCATCTCTCCAGCTTCCCAGTCCCCATTTTGATATTTTTATTCTACACATAGGAGAATACCTGCCTCTATCTCTGGAATGCTGGGATTATAGACACATGCCACCAAACCTAGCCTTATAACTTAATTTTTTAGAAAGATTTTTGCCAGTAGGGGTAGCACACAAATTTACCAG NGG 10 8 17 8 24 0 100 5 10
    260522155646_olut TTCTCTGACAGAACAAGTGGGGTTGGTTGGTTTTTAGTTTAAGACTAGGGTTTCTCAGTATAGCCTTGGCTACCCTCAGACTCCCCAG(+ctt)TGCAGGGATTAAAGCACCACTACCACCAGCCCAGAACACATGTTGGCCTAATTGCTGAACCATCTCTCCAGCTTCCCAGTCCCCATTTTGATATTTTTATTCTACACATAGGAGAATACCTGCCTCTATCTCTGGAATGCTGGGATTATAGACACATGCCACCAAACCTAGCCTTATAACTTAATTTTTTAGAAAGATTTTTGCCAGTAGGGGTAGCACACAAATTTACCAG NGG 10 8 17 8 24 0 100 5 10
    260522155626_dj8e TTCTCTGACAGAACAAGTGGGGTTGGTTGGTTTTTAGTTTAAGACTAGGGTTTCTCAGTATAGCCTTGGCTACCCTCAGACTCCCCAG(+ctt)TGCAGGGATTAAAGCACCACTACCACCAGCCCAGAACACATGTTGGCCTAATTGCTGAACCATCTCTCCAGCTTCCCAGTCCCCATTTTGATATTTTTATTCTACACATAGGAGAATACCTGCCTCTATCTCTGGAATGCTGGGATTATAGACACATGCCACCAAACCTAGCCTTATAACTTAATTTTTTAGAAAGATTTTTGCCAGTAGGGGTAGCACACAAATTTACCAG NGG 10 8 17 12 60 0 100 5 10
    260522155348_eq62 TGTCTGCTGAGCCAACAGTGGTAGTAAGGTAAGGGCAGGATGTGTCAAACTGCCAATAGAGAACTACTTACTCTTCAGGCTGAAGCTGATGGAACAGGTAACAAAGGCAAACACTAATCATGATCAGCAAGATGAAGCAGAAAGGGAACAAGGGGATATTAAATGTGTATAGACACGCTAGAG(+ggcttgtcgacgacggcggtctccgtcgt)AGATGGCTCAGCAGTTAAGAGAACTAGCTGGTCTTTCAGAGGTCCTGAGATCAATTTTAGACACCCACATGGTGGCTCATGACCATCTATCTATAAATGGATCTGATTTTCATGTCTGGCAGTGTACAGAAGCTAACTGAAGAAAGGTGGAAGACCCACAAGAGTTCAAGATAAGCCCTATATAGTGAAGTTCAAGGCAA NGG 10 8 17 12 60 0 100 5 10