Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250421184740_rlts TTATAAATTGAATGTAAAGCATTCTGAAAGATACTATACTGATTGCAGGTTTTTCAGTCAGGTTCAAGCTAATTTGACCAGTCATTGGATTAATTATGGATCTGGGGCCATAAATGCTATTTTAATTCCACTATAGAGATTAAAATAAGCCATTCTCCATTTCATAATATTCTATTGGACTTTGACTGCAGGGGCCTCCAAGTCTTGACAGTAGATTATAATCCTTCAGCTGCCCACTCTACTGGAGGAGGACAAACTGGTCACTTTTCAGCAAAACCTGGCTGTGGATCAGGGCAGTCTGGTACTTCCAAGC(+aag)TCATTAGATGCCATCATGCTCTCACTGCCTCCTCAGCTTCAAGAGGAATCTGGAAAAAGCAGTCCCACTGGTCAGGAAAGGAACACTAGTGCACTTATCCTGGGTGTCTGCTGAGCCAACAGTGGTAGTAAGGTAAGGGCAGGATGTGTCAAACTGCCAATAGAGAA NGG 10 8 17 8 24 0 100 5 10
    250421184716_jsf1 TTCATAATATTCTATTGGACTTTGACTGCAGGGGCCTCCAAGTCTTGACAGTAGATTATAATCCTTCAGCTGCCCACTCTAC(+ctt)TGGAGGAGGACAAACTGGTCACTTTTCAGCAAAACCTGGCTGTGGATCAGGGCAGTCTGGTACTTCCAAGCTCATTAGATGCCATCATGCTCTCACTGCCTCCTCAGCTTCAAGAGGAATCTGGAAAAAGCAGTCCCACTGGTCAGGAAAGGAACACTAGTGCACTTATCCTGGGTGTCTGCTGAGCCAACAGTGGTAGTAAGGTAAGGGCAGGATGTGTCAAACTGCCAATAGAGAA NGG 10 8 17 8 24 0 100 5 10
    250421184633_mr26 CACTCCAGAGGCAGAGGAAGGCAGAGTTGTACCTGGCCTACATAGTAACTTCCAGGACAGCCAGGGCTACATAGTGAAATCTTGTCTCAAAAAACAAAGTTTAATTAAGCTAGAGACATGAATTCTTTGTTAAGAGTGCTCAGTACACTTGGCAGAGGACCCAAGTTCAATTTCCAGCATACACCAGGCAGTAGTCACAACTACCCATAAGCTCCACCTAGC(+aag)CTCCAACACAGCCTGCATTCAGTACACACACACATATATAAGTTAAAGGTCTTTTAAAAACAAAAACGGGAGTTATGGTGCTGGAGAGATAGCTCAGGGTAAGAGCAGTGTATTGCTCTTGAGGGGACCTGGGTCCAGGCACACACTTGGCACACATACATACATGAAGACAAGACACTCAGAAGTAAATCTATAAAGGGGGTCACCTATTTTTATTTTCTGTGGGTATTCATGTCTCAGTGCATTGTGGTGCCAGGC NGG 10 8 17 8 24 0 100 5 10
    250421184613_xix6 CACTCCAGAGGCAGAGGAAGGCAGAGTTGTACCTGGCCTACATAGTAACTTCCAGGACAGCCAGGGCTACATAGTGAAATCTTGTCTCAAAAAACAAAGTTTAATTAAGCTAGAGACATGAATTCTTTGTTAAGAGTGCTCAGTACACTTGG(+ctt)CAGAGGACCCAAGTTCAATTTCCAGCATACACCAGGCAGTAGTCACAACTACCCATAAGCTCCACCTAGCCTCCAACACAGCCTGCATTCAGTACACACACACATATATAAGTTAAAGGTCTTTTAAAAACAAAAACGGGAGTTATGGTGCTGGAGAGATAGCTCAGGGTAAGAGCAGTGTATTGCTCTTGAGGGGACCTGGGTCCAGGCACACACTTGGCACACATACATACATGAAGACAAGACACTCAGAAGTAAATCTATAAAGGGGGTCACCTATTTTTATTTTCTGTGGGTATTCATGTCTCAGTGCATTGTGGTGCCAGGC NGG 10 8 17 8 24 0 100 5 10
    250421184553_jgo1 TTCTCTGACAGAACAAGTGGGGTTGGTTGGTTTTTAGTTTAAGACTAGGGTTTCTCAGTATAGCCTTGGCTACCCTCAGACTCCCCAGTGCAGGGATTAAAGCACCACTACCACCAGCCCAGAACACATGTTGGCCTAATTGCTGAACCATCT(+aag)CTCCAGCTTCCCAGTCCCCATTTTGATATTTTTATTCTACACATAGGAGAATACCTGCCTCTATCTCTGGAATGCTGGGATTATAGACACATGCCACCAAACCTAGCCTTATAACTTAATTTTTTAGAAAGATTTTTGCCAGTAGGGGTAGCACACAAATTTACCAG NGG 10 8 17 8 24 0 100 5 10
    250421184530_swx1 TTCTCTGACAGAACAAGTGGGGTTGGTTGGTTTTTAGTTTAAGACTAGGGTTTCTCAGTATAGCCTTGGCTACCCTCAGACTCCCCAG(+ctt)TGCAGGGATTAAAGCACCACTACCACCAGCCCAGAACACATGTTGGCCTAATTGCTGAACCATCTCTCCAGCTTCCCAGTCCCCATTTTGATATTTTTATTCTACACATAGGAGAATACCTGCCTCTATCTCTGGAATGCTGGGATTATAGACACATGCCACCAAACCTAGCCTTATAACTTAATTTTTTAGAAAGATTTTTGCCAGTAGGGGTAGCACACAAATTTACCAGCACTCCAGAGGCAGAGGAAGGCAGAGTTGTACCTGGCCTACATAGTAACTTCCAGGACAGCCAGGGCTACATAGTGAAATCTTGTCTCAAAAAACAAAGTTTAATTAAGCTAGAGACATGAATTCTTTGTTAAGAGTGCTCAGTACACTTGGCAGAGGAC NGG 10 8 17 8 24 0 100 5 10
    250421184453_moag ATTTTTGTTAGGATTTGTTTCTAAATGAGGGATTTTTTGGTGTTTTGTTTTACATATATAAGTGTGTACCATATGTATCTAGTGCCCAATGATCAGAAGAGGGCGTTGGGTCTCTTGATAATTATGAGCCACCATGG(+aag)GGGCATTGGAAACCAAATCCATGTTCTCTGACAGAACAAGTGGGGTTGGTTGGTTTTTAGTTTAAGACTAGGGTTTCTCAGTATAGCCTTGGCTACCCTCAGACTCCCCAGTGCAGGGATTAAAGCACCACTACCACCAGCCCAGAACACATGTTGGCCTAATTGCTGAACCATCTCTCCAGCTTCCCAGTCCCCATTTTGATATTTTTATTCTACACATAGGAGAATACCTGCCTCTATCTCTGGAATGCTGGGATTATAGACACATGCCACCAAACCTAGCCTTATAACTTAATTTTTTAGAAAGATTTTTGCCAGTAGGGGTAGCACACAAATTTACCAG NGG 10 8 17 8 24 0 100 5 10
    250421184428_dvrs ATTTTTGTTAGGATTTGTTTCTAAATGAGGGATTTTTTGGTGTTTTGTTTTACATATATAAGTGTGTACCATATGTATCTAGTGCCCAATGATCAGA(+ctt)AGAGGGCGTTGGGTCTCTTGATAATTATGAGCCACCATGGGGGCATTGGAAACCAAATCCATGTTCTCTGACAGAACAAGTGGGGTTGGTTGGTTTTTAGTTTAAGACTAGGGTTTCTCAGTATAGCCTTGGCTACCCTCAGACTCCCCAGTGCAGGGATTAAAGCACCACTACCACCAGCCCAGAACACATGTTGGCCTAATTGCTGAACCATCTCTCCAGCTTCCCAGTCCCCATTTTGATATTTTTATTCTACACATAGGAGAATACCTGCCTCTATCTCTGGAATGCTGGGATTATAGACACATGCCACCAAACCTAGCCTTATAACTTAATTTTTTAGAAAGATTTTTGCCAGTAGGGGTAGCACACAAATTTACCAG NGG 10 8 17 8 24 0 100 5 10
    250421184349_2bbr CCAAGTTCAATTTCCAGCATACACCAGGCAGTAGTCACAACTACCCATAAGCTCCACCTAGCCTCCAACACAGCCTGCATTCAGTACACACACACATATATAAGTTAAAGGTCTTTTAAAAACAAAAACGGGAGTTATGGTGCTGGAGAGATAGCTCAGGGTAAGAGCAGTGTATTGCTCTTGAGGGGACCTGGG(+aag)TCCAGGCACACACTTGGCACACATACATACATGAAGACAAGACACTCAGAAGTAAATCTATAAAGGGGGTCACCTATTTTTATTTTCTGTGGGTATTCATGTCTCAGTGCATTGTGGTGCCAGGCCTGGCTTTCACATGAATGCTGGAGACAGCGCAAGTCCTGATGCTTGAGGGGCAGACACTACCAAAAGACCAAAGGAATCAGCTCTCTAGCTTTCCAGTCACAGCTTTGATTTCCGAAGTTACTTCTTGATGAGAAATGTAACTGACTAGAACAATTCATC NGG 10 8 17 8 24 0 100 5 10
    250421184326_oezr CCAAGTTCAATTTCCAGCATACACCAGGCAGTAGTCACAACTACCCATAAGCTCCACCTAGCCTCCAACACAGCCTGCATTCAGTACACACACACATATATAAGTTAAAGGTCTTTTAAAAACAAAAACGGGAGTTATGGTGCTGGAGAGATAGC(+ctt)TCAGGGTAAGAGCAGTGTATTGCTCTTGAGGGGACCTGGGTCCAGGCACACACTTGGCACACATACATACATGAAGACAAGACACTCAGAAGTAAATCTATAAAGGGGGTCACCTATTTTTATTTTCTGTGGGTATTCATGTCTCAGTGCATTGTGGTGCCAGGCCTGGCTTTCACATGAATGCTGGAGACAGCGCAAGTCCTGATGCTTGAGGGGCAGACACTACCAAAAGACCAAAGGAATCAGCTCTCTAGCTTTCCAGTCACAGCTTTGATTTCCGAAGTTACTTCTTGATGAGAAATGTAACTGACTAGAACAATTCATC NGG 10 8 17 8 24 0 100 5 10