Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250421183652_mn6p ATTTTTGTTAGGATTTGTTTCTAAATGAGGGATTTTTTGGTGTTTTGTTTTACATATATAAGTGTGTACCATATGTATCTAGTGCCCAATGATCAGAAGAGGGCGTTGGGTCTCTTGATAATTATGAGCCACCA(+agg)TGGGGGCATTGGAAACCAAATCCATGTTCTCTGACAGAACAAGTGGGGTTGGTTGGTTTTTAGTTTAAGACTAGGGTTTCTCAGTATAGCCTTGGCTACCCTCAGACTCCCCAGTGCAGGGATTAAAGCACCACTACCACCAGCCCAGAACACATGTTGGCCTAATTGCTGAACCATCTCTCCAGCTTCCCAGTCCCCATTTTGATATTTTTATTCTACACATAGGAGAATACCTGCCTCTATCTCTGGAATGCTGGGATTATAGACACATGCCACCAAACCTAGCCTTATAACTTAATTTTTTAGAAAGATTTTTGCCAGTAGGGGTAGCACACAAATTTACCAG NGG 10 8 17 8 24 0 100 5 10
    250421183631_t4bp ATTTTTGTTAGGATTTGTTTCTAAATGAGGGATTTTTTGGTGTTTTGTTTTACATATATAAGTGTGTACCATATGTATCTAGTGCCCAATGATCAGA(+ctt)AGAGGGCGTTGGGTCTCTTGATAATTATGAGCCACCATGGGGGCATTGGAAACCAAATCCATGTTCTCTGACAGAACAAGTGGGGTTGGTTGGTTTTTAGTTTAAGACTAGGGTTTCTCAGTATAGCCTTGGCTACCCTCAGACTCCCCAGTGCAGGGATTAAAGCACCACTACCACCAGCCCAGAACACATGTTGGCCTAATTGCTGAACCATCTCTCCAGCTTCCCAGTCCCCATTTTGATATTTTTATTCTACACATAGGAGAATACCTGCCTCTATCTCTGGAATGCTGGGATTATAGACACATGCCACCAAACCTAGCCTTATAACTTAATTTTTTAGAAAGATTTTTGCCAGTAGGGGTAGCACACAAATTTACCAG NGG 10 8 17 8 24 0 100 5 10
    250421182830_r82b GCTTTTTCTACCTGAAACTTAGTCTCAAAAAAAAATGAATACGTAAACAGTCTTCCAGGGGATAAGAACCTTACAGAAAAAGCAGAAATGCCTGGGGCACTGGATTACCGATGTAATCAAATTCAGTCCTTGAATTGAACACAGGATTGCCTAGAGCAAGGCCAGCCAGAGATTCATCTCAGAGGGAGAAAGGTGTCTTTGGAGCAATTTTGTGGTAATCTAGTATGTATCACATAAGTTTAGACGCATTTGGGACTGGAAAGATGTGAACAAAGCACCCTATGGCTCACATCTGTCATTAACTCTAGTTCCAGTGCACCTGACACCGTCT(+aag)TCTGGCCTCTGCAGTGACCAAGCACATGGGTAGTATGTAGACATATACATAAGCAAAACACACATCATTAAAAAGTGACATTTCCCAAAGGAAGCTGAAGAACCAGTTCTTGAGAAGATAGTAGAAATCAGAAGGGGAAATAGTAGACA NGG 10 8 17 8 24 0 100 5 10
    250421182746_pipx GCTTTTTCTACCTGAAACTTAGTCTCAAAAAAAAATGAATACGTAAACAGTCTTCCAGGGGATAAGAACCTTACAGAAAAAGCAGAAATGCCTGGGGCACTGGATTACCGATGTAATCAAATTCAGTCCTTGAATTGAACACAGGATTGCCTAGAGCAAGGCCAGCCAGAGATTCATCTCAGAGGGAGAAAGGTGTCTTTGGAGCAATTTTGTGGTAATCTAGTATGTATCACATAAGTTTAGACGCATTTGGGACTGGAAAGATGTGAACAAAGCACCCTATGGCTCACATCTGTCATTAACTCTAGTTCCAGTG(+aag)CACCTGACACCGTCTTCTGGCCTCTGCAGTGACCAAGCACATGGGTAGTATGTAGACATATACATAAGCAAAACACACATCATTAAAAAGTGACATTTCCCAAAGGAAGCTGAAGAACCAGTTCTTGAGAAGATAGTAGAAATCAGAAGGGGAAATAGTAGACA NGG 10 8 17 8 24 0 100 5 10
    250421182715_eave GCTTTTTCTACCTGAAACTTAGTCTCAAAAAAAAATGAATACGTAAACAGTCTTCCAGGGGATAAGAACCTTACAGAAAAAGCAGAAATGCCTGGGGCACTGGATTACCGATGTAATCAAATTCAGTCCTTGAATTGAACACAGGATTGCCTAGAGCAAGGCCAGCCAGAGATTCATCTCAGAGGGAGAAAGGTGTCTTTGGAGCAATTTTGTGGTAATCTAGTATGTATCACATAAGTTTAGACGCATTTGGGACTGGAAAGATGTGAACAAAGCACC(+GAGAGTA)CTATGGCTCACATCTGTCATTAACTCTAGTTCCAGTGCACCTGACACCGTCTTCTGGCCTCTGCAGTGACCAAGCACATGGGTAGTATGTAGACATATACATAAGCAAAACACACATCATTAAAAAGTGACATTTCCCAAAGGAAGCTGAAGAACCAGTTCTTGAGAAGATAGTAGAAATCAGAAGGGGAAATAGTAGACA NGG 10 8 17 8 24 0 100 5 10
    250421182623_co43 GCTTTTTCTACCTGAAACTTAGTCTCAAAAAAAAATGAATACGTAAACAGTCTTCCAGGGGATAAGAACCTTACAGAAAAAGCAGAAATGCCTGGGGCACTGGATTACCGATGTAATCAAATTCAGTCCTTGAATTGAACACAGGATTGCCTAGAGCAAGGCCAGCCAGAGATTCATCTCAGAGGGAGAAAGGTGTCTTTGGAGCAATTTTGTGGTAATCTAGTATGTATCACATAAGTTTAGACGCATTTGGGACTGGAAAGATGTGAACAAAGCACC(+ctt)CTATGGCTCACATCTGTCATTAACTCTAGTTCCAGTGCACCTGACACCGTCTTCTGGCCTCTGCAGTGACCAAGCACATGGGTAGTATGTAGACATATACATAAGCAAAACACACATCATTAAAAAGTGACATTTCCCAAAGGAAGCTGAAGAACCAGTTCTTGAGAAGATAGTAGAAATCAGAAGGGGAAATAGTAGACA NGG 10 8 17 8 24 0 100 5 10
    250421182550_prj4 GCTTTTTCTACCTGAAACTTAGTCTCAAAAAAAAATGAATACGTAAACAGTCTT(+ctt)CCAGGGGATAAGAACCTTACAGAAAAAGCAGAAATGCCTGGGGCACTGGATTACCGATGTAATCAAATTCAGTCCTTGAATTGAACACAGGATTGCCTAGAGCAAGGCCAGCCAGAGATTCATCTCAGAGGGAGAAAGGTGTCTTTGGAGCAATTTTGTGGTAATCTAGTATGTATCACATAAGTTTAGACGCATTTGGGACTGGAAAGATGTGAACAAAGCACCCTATGGCTCACATCTGTCATTAACTCTAGTTCCAGTGCACCTGACACCGTCTTCTGGCCTCTGCAGTGACCAAGCACATGGGTAGTATGTAGACATATACATAAGCAAAACACACATCATTAAAAAGTGACATTTCCCAAAGGAAGCTGAAGAACCAGTTCTTGAGAAGATAGTAGAAATCAGAAGGGGAAATAGTAGACA NGG 10 8 17 8 24 0 100 5 10
    250421182506_qfc9 ATGCCATCATGCTCTCACTGCCTCCTCAGCTTCAAGAGGAATCTGGAAAAAGCAGTCCCACTGGTCAGGAAAGGAACACTAGTGCACTTATCCTGGGTGTCTGCTGAGCCAACAGTGGTAGTAAGGTAAGGGCAGGATGTGTCAAACTGCCAATAGAGAACTACTTACTCTTCAGGCTGAAGCTGATGGAACAGGTAACAAAGGCAAACACTAATCATGATCAGCAAGATGAAGCAGAAAGGGAACAAGGGGATATTAAATGTGTATAGACACGCTAGAGAGATGGCTCAGCAGTTAAGAGAACTAGCTGGTCTTTCAGAGGTCCTGAGATCAATTTTAGACACCCACA(+aag)TGGTGGCTCATGACCATCTATCTATAAATGGATCTGATTTTCATGTCTGGCAGTGTACAGAAGCTAACTGAAGAAAGGTGGAAGACCCACAAGAGTTCAAGATAAGCCCTATATAGTGAAGTTCAAGGCAA NGG 10 8 17 8 24 0 100 5 10
    250421182442_dfcg CTACTTACTCTTCAGGCTGAAGCTGATGGAACAGGTAACAAAGGCAAACACTAATCATGATCAGCAAGATGAAGCAGAAAGGGAACAAGGGGATATTAAATGTGTATAGACACGCTAGAGAGATGGCTCAGCAGTTAAGAGAACTAGCTGGTCTTT(+ctt)CAGAGGTCCTGAGATCAATTTTAGACACCCACATGGTGGCTCATGACCATCTATCTATAAATGGATCTGATTTTCATGTCTGGCAGTGTACAGAAGCTAACTGAAGAAAGGTGGAAGACCCACAAGAGTTCAAGATAAGCCCTATATAGTGAAGTTCAAGGCAAGCTTTTTCTACCTGAAACTTAGTCTCAAAAAAAAATGAATACGTAAACAGTCTTCCAGGGGATAAGAACCTTACAGAAAAAGCAGAAATGCCTGGGGCACTGGATTACCGATGTAATCAAATTCAGTCCTTGAATTGAACACAGGATTGCCTAGAGCAAG NGG 10 8 17 8 24 0 100 5 10
    250421182426_dfu6 CTACTTACTCTTCAGGCTGAAGCTGATGGAACAGGTAACAAAGGCAAACACTAATCATGATCAGCAAGATGAAGCAGAAAGGGAACAAGGGGATATTAAATGTGTATAGACACGCTAGAG(+ctt)AGATGGCTCAGCAGTTAAGAGAACTAGCTGGTCTTTCAGAGGTCCTGAGATCAATTTTAGACACCCACATGGTGGCTCATGACCATCTATCTATAAATGGATCTGATTTTCATGTCTGGCAGTGTACAGAAGCTAACTGAAGAAAGGTGGAAGACCCACAAGAGTTCAAGATAAGCCCTATATAGTGAAGTTCAAGGCAAGCTTTTTCTACCTGAAACTTAGTCTCAAAAAAAAATGAATACGTAAACAGTCTTCCAGGGGATAAGAACCTTACAGAAAAAGCAGAAATGCCTGGGGCACTGGATTACCGATGTAATCAAATTCAGTCCTTGAATTGAACACAGGATTGCCTAGAGCAAG NGG 10 8 17 8 24 0 100 5 10