Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    251021151821_snir GCTGGAGTGCAATGGTGCGATCTTGGCTCGGCACGATCTCGGCTCAGCACGATCTCAGCTTACTGCAACCTCCGCCTTTCGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCAAG(+CATCACCATCACCATCAC)TAGCTGGCATTACAGGCATGTGCCACCACGCCTGGCTAATTTTGTATTTTTTGTAGAGATGGGGTTTCTCCTTGTTGGTCAGGCTGGTCTTGAACTCCCAACATCAGGTGATCCGC NGG 10 8 17 8 24 0 100 5 10
    251021151752_zyg6 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    251021151556_tjct GCTGGAGTGCAATGGTGCGATCTTGGCTCGGCACGATCTCGGCTCAGCACGATCTCAGCTTACTGCAACCTCCGCCTTTCGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCAAG(+CATCACCATCACCATCAC)TAGCTGGCATTACAGGCATGTGCCACCACGCCTGGCTAATTTTGTATTTTTTGTAGAGATGGGGTTTCTCCTTGTTGGTCAGGCTGGTCTTGAACTCCCAACATCAGGTGATCCGC NGG 10 8 17 8 24 0 100 5 10
    251021135858_selx AAGGGGAGGGGGAGCGAGTGGTGGGAGGCCCGGCCCTGGGTCGGGGGGCGGCTGGGCAACGACCCCACGCGGCGACGGCCCCCCCCCGCCCCGCAGATGAGGAGCAGCTCTG(/A)GGCCGAAGTGTCGCAGGCCGGGACCGTCCTGGACAGCAACCACACGGTGGGCGTCCTGGCCAGCGCCCACCGCCCCCAGGGCCCGGCCGACGCCTGGCGCGCCGCGGTGCTGATCTACGCGAGCGACGACACCCGCGCCCACCCCAACC NGG 10 8 17 8 24 0 100 5 10
    251021135748_c0ee AAGGGGAGGGGGAGCGAGTGGTGGGAGGCCCGGCCCTGGGTCGGGGGGCGGCTGGGCAACGACCCCACGCGGCGACGGCCCCCCCCCGCCCCGCAGATGAGGAGCAGCTCTG(/A)GGCCGAAGTGTCGCAGGCCGGGACCGTCCTGGACAGCAACCACACGGTGGGCGTCCTGGCCAGCGCCCACCGCCCCCAGGGCCCGGCCGACGCCTGGCGCGCCGCGGTGCTGATCTACGCGAGCGACGACACCCGCGCCCACCCCAACC NGG 10 8 17 8 24 0 100 5 10
    251021134834_ziwa CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    251021130356_x9ng CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    251013153158_ye18 AACTGAGAAAGTATCAGGTGGGGGGGGGGGGGAGAAGACAGCATGTTTCCATAGGAAAATGGACCAAAAGTGCAAGTTTTGGTTCAACATTATTGAAATCCTACAGGTCATTGCTGAACGAGTCAAAGAAAGGAAGGCAGAGGAAGACTGGACGGGTGCTGGCAGGGGTCCTATC(+ag)CCCTCCAAAAATAAGCGCAAGGCTTTCCTTGACTTGCTTTTGAGTGTGACTGATGAGGAAGGAAACAGATTAAGCCAGGAAGACATCCGAGAGGAAGTTGACACCTTCATGTTTGAGGTAACTTTTGTCGCCAGGTAATTTTCCT NGG 10 8 17 8 24 0 100 5 10
    251013151540_potv GATTCTATTCCCCAGTTAGAAAGACTGCAAACTGGTACTTGAAAACTCTGCGTTTGTCCTGTCATTCCATTTGTAGTTAAGGGTTTCACCATATGACATCCTCGGTGTTAACCAGAGGCCTTGGTGCTGATTCACAAGAACCTAAGCTGTCACTGAAATGAACTGAGAAAGTATCAGGTGGGGGGGGGGGGGAGAAGACAGCATGTTTCCATAGGAAAATGGACCAAAAGTGCAAG(+ct)TTTTGGTTCAACATTATTGAAATCCTACAGGTCATTGCTGAACGAGTCAAAGAAAGGAAGGCAGAGGAAGACTGGACGGGTGCTGGCAGGGGTCCTATCCCCTCCAAAAATAAGCGCAAGGCTTTCCTTGACTTGCTTTTGAGTGTGACTGATGAGGAAGGAAACAGATTAAGCCAGGAAGACATCCGAGAGGAAGTTGACACCTTCATGTTTGAGGTAACTTTTGTCGCCAGGTAATTTTCCT NGG 10 8 17 8 24 0 100 5 10
    251013151349_lyg0 GATTCTATTCCCCAGTTAGAAAGACTGCAAACTGGTACTTGAAAACTCTGCGTTTGTCCTGTCATTCCATTTGTAGTTAAGGGTTTCACCATATGACATCCTCGGTGTTAACCAGAGGCCTTGGTGCTGATTCACAAGAACCTAAGCTGTCACTGAAATGAACTGAGAAAGTATCAGGTGGGGGGGGGGGGGAGAAGACAGCATGTTTCCATAGGAAAATGGACCAAAAGTGCAAGCATGTGATTATCATTCAAAgcGAACGGGAACGAGTCAAAGAAAGGAAGGCAGAGGAAGACTGGACGGGTGCTGGCAGGGGTCCTATC(+ga)CCCTCCAAAAATAAGCGCAAGGCTTTCCTTGACTTGCTTTTGAGTGTGACTGATGAGGAAGGAAACAGATTAAGCCAGGAAGACATCCGAGAGGAAGTTGACACCTTCATGTTTGAGGTAACTTTTGTCGCCAGGTAATTTTCCTTACTGTATTTCA NGG 10 8 17 8 24 0 100 5 10