Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250708142945_qp3d aaaaagaacaattttatcctcatccttggaagaatccattaccctatcaa(-a)ggaatttggatgttaatttatgtttggataagacttgtttatccattgtc NGG 10 8 17 8 24 0 100 5 10
    250707174015_sju0 GAGCATCCCCAGCCGCCAGTGGCACCACGCTCACCGTGGGGCTGCT(-CTGGGCACC)GCTCATCGCCGCTGCTCTGGGCACCGCTGCCTACACCTACCG NGG 10 8 17 8 24 0 100 5 10
    250704092946_wwjr CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250703170317_au8f CCTGGGAGTCGTTGGATCCACCACCACTCAGCTGTACACGGACCa(/G)CACGGAGAAGCTGAGGCCTGAGATGGAGGGGCCCGGCAGCTTCAC NGG 10 8 17 8 24 0 100 5 10
    250703154855_6y0c CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTCGCCGCCTCTACTGGGGCTT NGG 10 8 17 8 24 0 100 5 10
    250703154753_ud38 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+A)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250703152449_3kmr CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 13 18 8 24 0 100 5 10
    250703113118_2fg5 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250703111231_yl1w CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250702170352_w13z CTTCCCAGTGTGAGTGACATCCTAATATAATACTTCTAAATCTTAAGCTGCTTTGAGAAAGGCATGCAGCGTATTTATTGAAGGAATTGAAGATTTCTTACCCTACATAAGAATTCCAGTTAGGACAAGTTTATAGCAACAAACTTTCACGTTTGCTGTTAGTTCCTACCTGCGATTTTGAAGGAGTACAACTGAGAACAGCACTCAGTCTTGTATGTGTGTTGGGTCCTAGTCTGATTCATTTTTCTTATTGCTACCCTTATACCTCAGTCTCCAAGTAAAAA(+TGGACCTGGATAGTG)AGGAAATAACTCCTCCTTTGTAGATGTGTATATGTGAATGAATAGAATGGCATGTCCCACTTCAAATGTCTAGAAGTAGATGTTGGTGAAACATGCAATAAGAGCTGAGTTGCTCTGGACCTGGATAGTGGGCTGTAAGATACAGCACAGGAGTGTCCCAGGACTGTT NGG 10 8 17 8 24 0 100 5 10