Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250410180828_0807 GCTTCCAGATCAGTAGTTTTGCTCTGACCCTAAAATCAGACTCCCATCCCAATGAGTATCTACAGGGGAGGACCGGGCATTCTAAGCAGTTTACGTGCCAATTCAATTTCTTAACCTATCTCAg(/a)AGATGGAGATCAGTGTGAGTCCAATCCATGTTTAAATGGCGGCAGTTGCAAGGATGACATTAATTCCTATGAATGTTGGTGTCCCTTTGGATTTGAAGGAAAGAACTGTGAATTAGGTAAGTAA NG 10 8 17 8 24 0 100 5 10
    250409161905_sp9o TGGTACTCACTTTTCTAACTTATCCAAAACAACTTTCCACTGTTGCTTTCG(/A)GAGTGAGCAAGATAGAAACTGCTCCTTATGTGCAGACAACATTGAAGGAAAGACATTTGTTTCAACAGTAAATTCTTTAGTTTTT NGG 10 8 17 8 24 0 100 5 10
    250409161838_5qzt TGGTACTCACTTTTCTAACTTATCCAAAACAACTTTCCACTGTTGCTTTCG(/A)GAGTGAGCAAGATAGAAACTGCTCCTTATGTGCAGACAACATTGAAGGAAAGACATTTGTTTCAACAGTAAATTCTTTAGTTTTT NGG 10 8 17 8 24 0 100 5 10
    250409161737_anod TGGTACTCACTTTTCTAACTTATCCAAAACAACTTTCCACTGTTGCTTTCG(/A)GAGTGAGCAAGATAGAAACTGCTCCTTATGTGCAGACAACATTGAA NGG 10 8 17 8 24 0 100 5 10
    250409161725_1hsw CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250409084004_h1wz GGCACAATATTACACCTACAAGCGGCCACGGCAGTGGCTGTCTTCGGCTGGTCTGGGCGCAATGGGATTTGGGCTGCCTGCTGCAGCTGGTGCTTCTGTGGCTAACCCAGGTGTCACAGTTGTTGATATTGATGGGGATGGTAGCTTCCTCATGAACATTCAGGAGTTGGCATTGATCCGCATTGAGAACCTCCCGGTGAAGGTGATGGTGTTGAACAACCAACATTTGGGTATGGTGGTGCAGT(/A)TGGAGGATAGGTTTTACAAGGCAAATAGGGCGCATACATACTTGGGCAACCCAGAATGTGAGAGTGAGATATATCCAGATTTTGTGACTATTGCTAAAGGGTTCAATATTCCTGCAGTCCGTGTAACAAAGAAGAGTGAAGTCCGTGCCGCCATCAAGAAGATGCTCGATACCCCAGGGCCATACTTGTTGGATATCATCGTCCCACACCAGGAGCATGTGCTG NGG 10 8 17 8 24 0 100 5 10
    250409083436_684p CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250409082448_9fyv CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250409080354_fxr7 TGCAAAACACTATTTTACACTGTAGATAGCAATATTTATGTTGTTTAGAGAGTGAATTTTGAAATAAGAGGTGAGATAGAGAATGTGATATGGCAGCTAAGAGCTAGTTTTCAACAAGTTTGTTTCTATAATAGTATAGCTAGATAGACAGACACGTAGCTAAAATGGTCAACTGACCTTCGTACAAAAAAAAACTTTAATCCAGAGATATAGCAATATATGCTTATAACTCCAGACCTCAGAG(+ATTCTAGAAT)TCAACCCACAGATTTTTGTATATTCGTCAAAAAGATCCACAGATTTGTGGTAGAGAGTATGTTTTAGTAGTAGTGGTATATCTCCCTTGTGGGACGACGAAGCAGCCGGTCAGCCGGCGCTCCGGCCGGCCGGCCGGCCTGCGCCTGCACAGTACAAATAGCACCCCCCGTCCTCCAGTTGGCTGCATCCTTCTAGCTTCGATCTCTATATACCTCTGTATGTGAGTGAGGCCAATGAGAGCCCTCGTAGTCGTAAGTTTCGCTTCGGCGTGCTTGCTGCTGCTGTTGCAGCTCGCAGGAGCGTCGCATGTCGTCTACAACTACAAGGACCTCGAAGCCGAGGCTGCTGCGGCGACGGACCAGGTGCCGCCGTCCATCGTCAACCCCCTGCTCAGGACGGGGTACCACTTCCAGCCCCCCAAGAACTGGATCAATGGTAATGTAAAGCTACTAACTAATCCACCACCCAACGTCGTTTGAAGGTGATGTGTGTGTTAAGCATCTCCTGAAATATATAAGAGAGCGAGGCTAGTAATCGGCTTGTTGTTCAGGGTTTTGGTTTACCACTTGTAGCCTCAACTAATAAAGCTATATATATGAGAGCGACGAGAGAGAAGAGACAAAACATTCATGAGTATGGTCAGACAGACTAGCTAGCTAGACACGGCAGAGAAATTACGAGAGTTAAAAAAAATGCGCTTGAAGGGTGAACCAAAACAAAACAACTTTAGTACTATGCGTCAACAAAGCTGTGTTCATGTGTCAGCTAGCACTAGAGTCATAGTGCGTGGCCAGTGAGCTGTTGTCTAGCAACCAACGCGAACTGAAGTTTGAGAGCGTACTCGTGTTCTCGATCTCTTGCAGCGCAAAGGTCTTCGTACGTGATCAGGAATATTGCACCATTTACTGCTTAATTAATTAAGTACGTACGTACTGTACGTTTGGCTCTACCTCATCTTCTAATCTTTTCGGTGCTGTCTTGTGCTCTCTCGGCTCTGATTGCATCGATCGGCGGCGGCGTGAACTTTGCAACGGCGGCAGATCCCAACGGTAATAAGTCGTTTTCCCCACCCTTTTTATTTCCTCTAATAATGCATCAATATTCCAACTGGGTGCATATGCATGCGCGCAGCGCCCATGTACTACAAGGGGTGGTACCATTTCTTCTACCAATACAATCCCAAGGGCGCCGTATGGGGCAACATCGTGTGGGCGCACTCGGTGTCGCGAGACCTCATCAACTGGGTGGCGCTAGAGCCGGCGCTCAGACCAAGCATCCCGGGCGACAGGTACGGCTGCTGGTCCGGGTC NG 10 8 17 8 24 0 100 5 10
    250408223957_amui AGAGGCCAAGGAGACCACCACCTCCTTGCCTTCTCAGCTCACAAGTCTTGAGATCCACTGCTCAACTATGCACTAGGAATCAAGAGTCTTACCATCCAGAACCATGACTCTTCTTACTGAGGACCAGCCTTGCAGGTTCTCTGCACCGGAAAGCCCAATTGCATCCATGGAGCAGTACCAGGTGGACTCGGAGCTTAAAGAGGACTGTTTGGACAACATGGAAGCGGCTGTTTTTGGAGTGGTGGAGCCTCCGAGGCGCTCTAGGGGAAGGTTGATTATGCAC(-GG)GTCGGCCATGTTTGGAAGGGAATTCTGCTACGCTGTTGAGGCTGCGTTTGTCACGCCAGTGTTGCTGAGCGTTGGACTTCCCAGACGTCTGTACAGTCTGGTGTGGCTCATAAGCCCCATTTTGGGTTTTATCC NGG 10 8 17 8 24 0 100 5 10