Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    260430154524_fz9d atgagccatattcaacgggaaacgtcttgctctaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatca(+ggtgcgacaatctatcg)gtatgggaagcccgatgcgccagagttgtttctgaaacatggc NGG 10 8 17 8 24 0 100 5 10
    260430154513_aeqv atgagccatattcaacgggaaacgtcttgctctaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatca(+ggtgcgacaatctatcg)gtatgggaagcccgatgcgccagagttgtttctgaaacatggc NGG 10 8 17 8 24 0 100 5 10
    260430154144_vfva atgagccatattcaacgggaaacgtcttgctctaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgg(+gctcgcgataatgtcgg)atcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggc NGG 10 8 17 8 24 0 100 5 10
    260430153818_23n6 atgagccatattcaacgggaaacgtcttgctctaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgg(+gctcgcgataatgtcgg)atcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggc NGG 10 8 17 8 24 0 100 5 10
    260430153603_uobv atgagccatattcaacgggaaacgtcttgctctaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgat(+t)gtatgggaagcccgatgcgccagagttgtttctgaaacatggc NGG 10 8 17 8 24 0 100 5 10
    260430153507_8go7 tttttttgggctagcgattgaaaacgatgagccatattcaacgggaaacgtcttgc(+at)tctaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggc NGG 10 8 17 8 24 0 100 5 10
    260430153400_c1xi tttttttgggctagcgattgaaaacgatgagccatattcaacgggaaacgtcttgc(+at)tctaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggc NGG 10 8 17 8 24 0 100 5 10
    260430144700_dkr4 acccgtttttttgggctagcgattgaaaacgatgagccatat(+tcaacgggaaacgtctt)ctaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggc NGG 10 8 17 40 80 0 100 30 10
    260430144603_7mmu acccgtttttttgggctagcgattgaaaacgatgagccatat(+tcaacgggaaacgtctt)ctaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggc NGG 10 8 17 50 100 0 100 40 10
    260430144515_vwrf acccgtttttttgggctagcgattgaaaacgatgagccatat(+tcaacgggaaacgtctt)ctaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggc NGG 10 8 17 20 50 0 200 20 10