Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250408203642_fyxh TGGACTCGGAGCTTAAAGAGGACTGTTTGGACAACATGGAAGCGGCTGTTTTTGGAGTGGTGGAGCCTC(-C)GAGGCGCTCTAGGGGAAGGTTGATTATGCACGGGTCGGCCATGTTTGGAAGGGAATTCTGCTACGCTGTTGAGGCTGCGTTTGTCACGCCAGTGTTGCTGAGCGTTGGAC NGG 10 8 17 8 24 0 100 5 10
    250408203524_5bli TGGACTCGGAGCTTAAAGAGGACTGTTTGGACAACATGGAAGCGGCTGTTTTTGGAGTGGTGGAGCCT(-CC)GAGGCGCTCTAGGGGAAGGTTGATTATGCACGGGTCGGCCATGTTTGGAAGGGAATTCTGCTACGCTGTTGAGGCTGCGTTTGTCACGCCAGTGTTGCTGAGCGTTGGACTTCCCAGACGTCTGTACAGTCTGGTGTGGCTCATAAGCCCCATTTTGGGTTTTATCC NGG 10 8 17 8 24 0 100 5 10
    250408203428_4ata ACCAGCCTTGCAGGTTCTCTGCACCGGAAAGCCCAATTGCATCCATGGAGCAGTACCAGGTGGACTCGGAGCTTAAAGAGGACTGTTTGGACAACATGGAAGCGGCTGTTTTTGGAGTGGTGGAGCCT(-CCG)AGGCGCTCTAGGGGAAGGTTGATTATGCACGGGTCGGCCATGTTTGGAAGGGAATTCTGCTACGCTGTTGAGGCTGCGTTTGTCACGCCAGTGTTGCTGAGCGTTGGACTTCCCAGACGTCTGTACAGTCTGGTGTGGCTCATAAGCCCCATTTTGGGTTTTATCC NGG 10 8 17 8 24 0 100 5 10
    250408203409_kitn ACCAGCCTTGCAGGTTCTCTGCACCGGAAAGCCCAATTGCATCCATGGAGCAGTACCAGGTGGACTCGGAGCTTAAAGAGGACTGTTTGGACAACATGGAAGCGGCTGTTTTTGGAGTGGTGGAGCCTCCGA(+TT)GGCGCTCTAGGGGAAGGTTGATTATGCACGGGTCGGCCATGTTTGGAAGGGAATTCTGCTACGCTGTTGAGGCTGCGTTTGTCACGCCAGTGTTGCTGAGCGTTGGACTTCCCAGACGTCTGTACAGTCTGGTGTGGCTCATAAGCCCCATTTTGGGTTTTATCC NGG 10 8 17 8 24 0 100 5 10
    250408203332_oqw4 ACCAGCCTTGCAGGTTCTCTGCACCGGAAAGCCCAATTGCATCCATGGAGCAGTACCAGGTGGACTCGGAGCTTAAAGAGGACTGTTTGGACAACATGGAAGCGGCTGTTTTTGGAGTGGTGGAGCCTCCG(+TT)AGGCGCTCTAGGGGAAGGTTGATTATGCACGGGTCGGCCATGTTTGGAAGGGAATTCTGCTACGCTGTTGAGGCTGCGTTTGTCACGCCAGTGTTGCTGAGCGTTGGACTTCCCAGACGTCTGTACAGTCTGGTGTGGCTCATAAGCCCCATTTTGGGTTTTATCC NGG 10 8 17 8 24 0 100 5 10
    250408203318_ikgp ACCAGCCTTGCAGGTTCTCTGCACCGGAAAGCCCAATTGCATCCATGGAGCAGTACCAGGTGGACTCGGAGCTTAAAGAGGACTGTTTGGACAACATGGAAGCGGCTGTTTTTGGAGTGGTGGAGC(+TT)CTCCGAGGCGCTCTAGGGGAAGGTTGATTATGCACGGGTCGGCCATGTTTGGAAGGGAATTCTGCTACGCTGTTGAGGCTGCGTTTGTCACGCCAGTGTTGCTGAGCGTTGGACTTCCCAGACGTCTGTACAGTCTGGTGTGGCTCATAAGCCCCATTTTGGGTTTTATCC NGG 10 8 17 8 24 0 100 5 10
    250408203308_xh30 ACCAGCCTTGCAGGTTCTCTGCACCGGAAAGCCCAATTGCATCCATGGAGCAGTACCAGGTGGACTCGGAGCTTAAAGAGGACTGTTTGGACAACATGGAAGCGGCTGTTTTTGGAGTGGTGGAGCC(+TT)TCCGAGGCGCTCTAGGGGAAGGTTGATTATGCACGGGTCGGCCATGTTTGGAAGGGAATTCTGCTACGCTGTTGAGGCTGCGTTTGTCACGCCAGTGTTGCTGAGCGTTGGACTTCCCAGACGTCTGTACAGTCTGGTGTGGCTCATAAGCCCCATTTTGGGTTTTATCC NGG 10 8 17 8 24 0 100 5 10
    250408203259_liir TCAACTATGCACTAGGAATCAAGAGTCTTACCATCCAGAACCATGACTCTTCTTACTGAGGACCAGCCTTGCAGGTTCTCTGCACCGGAAAGCCCAATTGCATCCATGGAGCAGTACCAGGTGGACTCGGAGCTTAAAGAGGACTGTTTGGACAACATGGAAGCGGCTGTTTTTGGAGTGGTGGAGCC(+TT)TCCGAGGCGCTCTAGGGGAAGGTTGATTATGCACGGGTCGGCCATGTTTGGAAGGGAATTCTGCTACGCTGTTGAGGCTGCGTTTGTCACGCCAGTGTTGCTGAGCGTTGGACTTCCCAGACGTCTGTACAGTCTGGTGTGGCTCATAAGCCCCATTTTGGGTTTTATCC NGG 10 8 17 8 24 0 100 5 10
    250408203236_73u3 ACCAGCCTTGCAGGTTCTCTGCACCGGAAAGCCCAATTGCATCCATGGAGCAGTACCAGGTGGACTCGGAGCTTAAAGAGGACTGTTTGGACAACATGGAAGCGGCTGTTTTTGGAGTGGTGGAGCCT(+TT)CCGAGGCGCTCTAGGGGAAGGTTGATTATGCACGGGTCGGCCATGTTTGGAAGGGAATTCTGCTACGCTGTTGAGGCTGCGTTTGTCACGCCAGTGTTGCTGAGCGTTGGACTTCCCAGACGTCTGTACAGTCTGGTGTGGCTCATAAGCCCCATTTTGGGTTTTATCC NGG 10 8 17 8 24 0 100 5 10
    250408203209_a6p4 AGAGGCCAAGGAGACCACCACCTCCTTGCCTTCTCAGCTCACAAGTCTTGAGATCCACTGCTCAACTATGCACTAGGAATCAAGAGTCTTACCATCCAGAACCATGACTCTTCTTACTGAGGACCAGCCTTGCAGGTTCTCTGCACCGGAAAGCCCAATTGCATCCATGGAGCAGTACCAGGTGGACTCGGAGCTTAAAGAGGACTGTTTGGACAACATGGAAGCGGCTGTTTTTGGAGTGGTGGAGCCT(+T)CCGAGGCGCTCTAGGGGAAGGTTGATTATGCACGGGTCGGCCATGTTTGGAAGGGAATTCTGCTACGCTGTTGAGGCTGCGTTTGTCACGCCAGTGTTGCTGAGCGTTGGACTTCCCAGACGTCTGTACAGTCTGGTGTGGCTCATAAGCCCCATTTTGGGTTTTATCC NGG 10 8 17 8 24 0 100 5 10