Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250908090534_8s857z5o AAGAGAGAGTTTAAAAAGAAGAGGAAATATTTGATGTGTTAATATAGCTGAGAGTTTTCCATATTTAATGGGAAATCCAATCCACGGATTTAGGAAACACC(/T)ACAAATCCTAATTTGGATAAATAAAAAGAAATCCACACCTGGTCACATGTTAATTAAACCAAAGAACACTAGAGACAAAAAAATCTTTAGAACAGTCAGAG NG 10 8 17 8 24 0 100 5 10
    250908090534_8s8574ev CTCATTCAGGCAGAGAGAAGAATAACATGCCCCCTTCCAAAAAAGACAAAACACAAGGTAGCCACAAATTCCCTAGACACATTAGAAAAATAGGGAGTGGA(/G)GAGGGGCATTGGAATGAAAGTTCTCTATCTCATTAGCTGTTAGGCTTTAGGCATTTATTTGGATGGATCCCTTTGGCACCTTGGAAGCACCACTGAAGTGC NG 10 8 17 8 24 0 100 5 10
    250908090534_8s8550av ACGCTGTGATTGGTCTCTTTCAGATCTGCAGACACTCAGTTGACACTTGTTATATCATGGGACCCCGGAAATTGGAGTGAAGCTAGAAACAGAAAACCCAT(/C)GCAGGGCCTCGGATTCCCACAAATGTGACAAGAGGTATAGGGAGTGAGTCACAGCGCTTTGCTCGTGACCCTGGGATCAGAGCACCCATCAGGCTTCCATT NG 10 8 17 8 24 0 100 5 10
    250908090534_8s854yhh GGTGGGGGTGGATGTAGCTGCAGAAAGCATGCACAACTTTGTGAAAGAGGCCCTGCCTTGTGCATGTCCATAGTGAGGCTACAGATGGCTTATTGTATATA(/C)ATTACAATGTAAATAGCTTTTTATTTCCTAAGAAATAATTTAATGTTTAGTAAAAAAGAAAACAGAAAAAAGAAAGATGCGTGTGTTGGCTTACGCACTGG NG 10 8 17 8 24 0 100 5 10
    250908090534_8s853ex8 GGTATGGGCTCTTGATTCTGTGACAATTTTTTTGATCCATCTATTTCTCTCACTCCTGGCTTTCTAGCCACATTCTGCACCTCCTTCCTGCTTTCCTAGAG(/T)CCTCGGTATGCTTTCCCTCAACCGACCCTGGCTCAGGAGGTTGATGCCATGGGAACCTGAACCTGAAACACTTCATGGTAGTAATTTCCTGTTTTCCTTGC NG 10 8 17 8 24 0 100 5 10
    250908090534_8s8528kw TTTGCTTTCCTGCTGCTGTATGTCTTTTGCGTCATCTTCGCATATTTAACCTCCTTGGCTCAACTGGGAACTCTTTGAAGGCTAGGGCCATCAATTACATT(/C)TTTGTTTCTTCCAGAAAATTGGCACGTACTGTGGAGTAATAACAACATTAGTACTAACAAACACTTACACACTTTCACAGAATTGTCAAGGACTACGAAGG NG 10 8 17 8 24 0 100 5 10
    250908090534_8s850non AATATTCGTTGGGTTTCATGTTAAGAAGCCTGTGGTCTAGGAGTGCTATTCAGTGTTTCTTTTCCTGATAAACACTTTGAATATTTTTTTTGTGTTTTTGT(/C)TTCCTTTTCTGAAGCTGTTCCTCCTTTTAAATATTTTTAATCACATTGATAAAATCTATCCTTCACCACCTCTGGTTCTACTATAGTTGATTTTTATTTTA NG 10 8 17 8 24 0 100 5 10
    250908090534_8s850ibq GAAGAAACCTAAAAACTGTGGATAGTGGAAAGATTTTTAGATTTTTTTTTTCCTTGGGGAAACTGGCAGGCAATGGGGGTTAGGGAGGTTGGGGCGGTGGG(/T)GGCTTTCTTGAGTTAAAGGGGCTTATATGTGATGTCAATATTTCTTCCTCTGAGAAATGGTATATATATGTGTATAATGTAAGTGTGTGCATGCATGTGCG NG 10 8 17 8 24 0 100 5 10
    250908090534_8s8508lv GAGACACCGCTGCTTAGCACCCCCAGCCAGAACACCCTGAGGGTCTCGGGGCTCTGGAGAGAGTGGGGCGGGAGGAAGAATTGGCACCTTCCTAGGGAAGG(/A)AGACGAGCGCTTCGCCTTGATTCTCCGAGAAGCCTCCGAGAAGTGCTTTAAGTGTGTTTGCATGCGCCAGGCGGTGGGCAGCGGGGGCCTGTCCAGCCCTC NG 10 8 17 8 24 0 100 5 10
    250908090534_8s8503pr CAAAAGATATCCCTGTTTTCCTAATAATGTAAACAGTAAAACTGAGGGTTTCAGTTAAAGGGATAATTCGTAACAAAATCCCATTGTTGAAACAATGGTCA(/C)AACATAAACATCTTATAATTCAGATCTGCATTTGGTAACAGGAGAATTGAGAGTTTTGATGCTTTGCTGGTTTTCCCACAGTGGGAGATGTAGATACCATT NG 10 8 17 8 24 0 100 5 10