Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250908090318_jkfamjwj ACAACTAAACTCAACCCATTATGAGGCAATCTACACTGTCCATACCTATGCTTAGACAGTAGGTGTAAGATTGATAAAAGTAACATTGTTTATTACAAGAC(/T)TGAGAAATTGCTTTTAGCTCTGGGCGAGATGCCCTGAGATTCCAATGCCAATGCTAGATGAGTGATACTGGTATGCCTGCCTTAGCAGTGGGTAATTTGAA NG 10 8 17 8 24 0 100 5 10
    250908090318_jkfam1i1 CTAAAAGAATATATAAAGCATTTACTTTAGGAAGAAGGAAAATGATCCTAAAAGGAAGAACCAAGAAGCAAGTAGCAATAGTGAGGCAATTGTGAAAATGT(/C)AGGTAAGTCTAAACACACTCTGTCTACTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTATTTTGAGACTGAGTCTTGCCCTGTCACCCAGACTGGAGTGC NG 10 8 17 8 24 0 100 5 10
    250908090318_jkfalsed GGCCTCGTCCTAACATGTCCCAAAGGGGGAGAACAGTGGGTGGTGATTTGAATGAGGAACTATTGCTTTCAGTACCAAAATAAGCAGATAGAGATAGAGAC(/G)CGATTCATCTTGGCAACATGTGTTTATTTGTTGGTCAGTGCAGGTTCTGATAGGTCTATTACTTCTCTATAATAAGACAGTGTAAAAATAAACTATGTTAT NG 10 8 17 8 24 0 100 5 10
    250908090318_jkfalmzn GTCAACAAACTGTTCGTTTTTAAGTTACAAATTTGAATTTAATGTTGTCATCATCGTCATGTGTTTCCCCAAAGGGAAGCCAGTCATTGACCATTTAAAAA(/C)GTCTCCTGCTAAGTATGGAAATCAGACAGTAAGAGAAAGCCAAAAAGCAATGCAGAGAAAGGTGTCCAAGCTGTCTTCAGCCTTCCCCAGCTAAAGAGCAG NG 10 8 17 8 24 0 100 5 10
    250908090318_jkfakl5w AAGAGAGAGTTTAAAAAGAAGAGGAAATATTTGATGTGTTAATATAGCTGAGAGTTTTCCATATTTAATGGGAAATCCAATCCACGGATTTAGGAAACACC(/T)ACAAATCCTAATTTGGATAAATAAAAAGAAATCCACACCTGGTCACATGTTAATTAAACCAAAGAACACTAGAGACAAAAAAATCTTTAGAACAGTCAGAG NG 10 8 17 8 24 0 100 5 10
    250908090318_jkfak7lu TGGAAAATGACCTCAAGGAAGCTACGGGCACAACTGGAAGCTTTGTAGAAATTAAACATATTCAAGGAGCAAGAAATGAACTTTCAGCAGTCTAAATTTTC(/G)TAAATAACCAATAGTTGCCAATCTAAAATGGCAGAGAAGATGAAATTTGATAAACTGAATTTTTTTTAAAAATCCATTTACCCTACAGGTTTGCATTTGTT NG 10 8 17 8 24 0 100 5 10
    250908090318_jkfajgbh GAGCCCAGCAAATAATTATGGGGTCCCTGTTAGTGAATTAAATCAATTCTGCATCATCCACATATGGATTAGCCATGTTGTGGACAGTGCAAGTACTTTTC(/T)AAATCTCCAGATTTCCCTATTACTCAAGAGCTGGTGATCTGAAACAAGAAAATTTGAGCTGTTCAAGTCCCAACAGCTTAATTTTGTGTGTCCATGGGCAC NG 10 8 17 8 24 0 100 5 10
    250908090318_jkfaixaq AGGCAGATCTGGGGCCCCTGTGCTGGCAAATCTCATCTTAGCCTCTGACAGGGAACGGGGCAGCATCAGGCCTCCACAGCCAGGCAAAAGGGGAAGGCCAC(/T)GAGCCCCACCAAGCACAAGGGTTCAGACTGCCTTCAACCCTTAAGGGGCACTTTTTCTCTCAAGGCTCAGAATAGACAACTGTGCCAAAAAGCAGCATGGT NG 10 8 17 8 24 0 100 5 10
    250908090318_jkfaijev CCCTCCGGCGCTCATGTTCCGAGTCTGCAGCTCCACACTTTAAATCATACTCGAATGCAGGACAAGTCCTAAATATTTGCCCCTTCCCTTGCAGTAATTTA(/G)TTTTCTGTATGGACTGTCCCTCCCCCAGAGGCTTCCCCACCCTCCATTGCCTGGCCCAGGTGAGCAGCTTGGCAGGTCTCCAAGTGGAACCCGGTCCAGAC NG 10 8 17 8 24 0 100 5 10
    250908090318_jkfaid7o AACTCATATCATTTTTTGCAATCATAAAAATAAGCAAAATAAAATAAAAACATTTCATGCTCATTAAACAAATTTTAGCCAATAGAGAATAGTGGAAAACC(/A)AAACAGCCAAAATCTTATCAATAAAACCACCTCTGTTTAGTATTTTGAGAGAATTATTATTATATTTTTGGCGATGGGGTTTCACTATGTTGCTTAGGCTG NG 10 8 17 8 24 0 100 5 10