Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250908090318_jkfaeg7g TTCCTTCAAGTATTACTCCTATAGTATTTTCTCCCATAGCTGTCTTCATCTGTGTATTTTAATAATGATCTTAGGATGGAGCAGAACATGGAGAGGAAGAT(/C)TTCATTTTAAGCTCCTCCTTTTCTTTGAAATACAATAATTTATATAGAAATGTGTAGCAGCAAATTATATTGGGGATTAGAATTTTGAATTAATAGCTCTC NG 10 8 17 8 24 0 100 5 10
    250908090318_jkfad14o ATTATTCTGCCTGCCAGAACGCAGTAACCCAGTGTCTCACAGAGCACAAGGGGTGTGCCACTGGTGGTACACAAGATAATTTTTAAGTAGTTTCTAGGAAC(/T)AACATTAAGTAATACCAAATCACAAAGAATGTTTCCCCTTTTCTATTCTTTTTTCATCCTGATTACAGCAAGGAAAAAGTCTCTGTTTAGTGCTAGCAGGT NG 10 8 17 8 24 0 100 5 10
    250908090318_jkfacfpm TACTTCAATTAAGTTCTCCTCTAAACTTTTAGGTCATTGTTTATATAGCAGAAAATTCAATGTTAGCGGATGGAAAACTGCTTCTTGAATAACCTTGATAG(/A)GTCATCCCTGAGTGCACCTCAGGTTCTCTCTTTACCTGGGCTTGTATCTTTTTTTTTTTTTTTTTTTTTTTTTTGAGACAGAGTTTTGCTCTTGTCGCCCA NG 10 8 17 8 24 0 100 5 10
    250908090318_jkfac42c ACAGATACAATGTTGAATAAGGCAAAGCACCTGCCCTCAGGTAGCTTGCAGTCAGGGAGGTAAGGGTAGTGGGCAGAGAGACCTGGAAACAGATATTAGAC(/A)CTGCACTAAGCATGTGTGGTTATTGAACAGTAAAAATGCCACCACAAATTGCGATATGATGTAAGTAAAATGCGTACTGGCTATTGAAGACTTGGCACAGA NG 10 8 17 8 24 0 100 5 10
    250908090318_jkfac21w TTTAAGGCATATAGAAACACAATCCTCTCATCCTTATTTCATATATTTCCATCACTGAAACAAAAATTTACCTGAACCCCAGAACCACCATCAAAATGAAG(/C)TTTCATGGAAAGGATATAGGATCTAAAAGTAATGGGGACAGGGTATGGTATGCATATTTCTATTCTTCTGTTATTGGATACAAAGCACTTTGTTTGCTAAA NG 10 8 17 8 24 0 100 5 10
    250908090318_jkfab0qo AGGAGCTAACTGCTAGGCTGGAAAATACGGGAGTTTTAGAATGCTTTGGAATTCAGGTGGGTCATACACTACTCAATTTGTGTTTCATGTGGTGTGGATTC(/G)TAGGGAACTGTTTCAGAGAGAAATAAGAGATGTAAACATAGATTATTTATGTTGATTACTCTTACACACTCAAGTAACAGAAATGATAAAGGAAAATAGAA NG 10 8 17 8 24 0 100 5 10
    250908090318_jkfaagh1 CTCAACCCATTCTGTCAGTACTACTGTGTGACACTTACAGGATTAAGTTCTACATTTGTGCATTCATGGTTGGTTCAGTGACTGCCAGCGAGGTAGCAGTG(/C)GCTCTCATCATAATGGCCTTTTCATTCCAGGACCTGGTGGCAAATGGGGCCCTTGTCAGCATCTGTAACAAGTATGGAGAGATGCCTGTGGACAAAGCCAA NG 10 8 17 8 24 0 100 5 10
    250908090318_jkfa9zvj TTTGGAACTGGGACATGATTCTATTTGTTATAAAATAAAATTGATGTGATTGTCACCTTATTTGAATAACTGTAATTCTTGTTTTACTGAAAGTATGTGCG(/A)TTTTCTTTTCATTTAATTTTTAAAAGTGCATACAATTCCATTCATAAAAGCTAAATTAGCCTGACCAACATGGTGAAACCCCATCTCTACTAAAAATACAA NG 10 8 17 8 24 0 100 5 10
    250908090318_jkfa9lv3 CACTTAGCTTCTCTAGTTTCTTATGATTTCCTATTTTACACTTAACAGCAAAAGGAGGCTTCCATTTAGAAGGCACTATCTCAAACATAAATAATTTCATA(/G)TTTATTTTATCACTGTAACACTAAGGAGCTATGGCATTAAAATATAAAACTATTTGGAACTTAATGTAAACCTTTGCACATGCAGACTATATAAAGGGAAT NG 10 8 17 8 24 0 100 5 10
    250908090318_jkfa8frp CTGAGTCTAGGGCCAAACAAGAGAACACTAGAGTGGGTTTCCTAAAGCATTAGTTCATACATCGCTCCAAAGTTGTTAGGCTCTAAGCACTGATGAGGTAC(/G)AAGCTAGCTGGCATTAGAGTCCAGATGTTACTATTAAGCGTTCTTTTAAATGAACACGGAGGAATGCTTCCATTCAGACAGCCCCAGTAAGTACACCCTTG NG 10 8 17 8 24 0 100 5 10