Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250610170617_tni7dk8m CGACTGCCCCAAGGGTTCAAGAACTCCCCGACCATCTTTGACGAAGCC(+TAGTGA)CTACACAGGGACCTGGCCAACTTCAGGATCCAACACCCTCAGGTGACCCTCCTCCAGTACGTGGATGACCTGCTTCTGGCGGGAGCCACCAAACAGGACTGCTTAGAAGGTACGAAGGCACTACTGCTGGAATTGTCTGACCTAGGCTACAGAGCCTCTGCTAAGAAGGCCCAGATTTGCAGGAGAGA NGG 10 8 17 8 24 0 100 5 10
    250610170617_tni7dbyw CGACTGCCCCAAGGGTTCAAGAACTCCCCGACCATCTTTGACGAAGCCCTACACAGGGACCTGGCCAACTTCAGGATCCAACACCCTCAGGTGACCCTCCTCCAGTACGTGGATGACCTGCTTCTG(+TAGTGA)GCGGGAGCCACCAAACAGGACTGCTTAGAAGGTACGAAGGCACTACTGCTGGAATTGTCTGACCTAGGCTACAGAGCCTCTGCTAAGAAGGCCCAGATTTGCAGGAGAGA NGG 10 8 17 8 24 0 100 5 10
    250610170617_tni7dat1 CGACTGCCCCAAGGGTTCAAGAACTCCCCGACCATCTTTGACGAAGCCCTACACAGGGACCTGGCCAACTTCAGG(+TAGTGA)ATCCAACACCCTCAGGTGACCCTCCTCCAGTACGTGGATGACCTGCTTCTGGCGGGAGCCACCAAACAGGACTGCTTAGAAGGTACGAAGGCACTACTGCTGGAATTGTCTGACCTAGGCTACAGAGCCTCTGCTAAGAAGGCCCAGATTTGCAGGAGAGA NGG 10 8 17 8 24 0 100 5 10
    250610170617_tni7d3v1 CGACTGCCCCAAGGGTTCAAGAACTCCCCGACCATCTTTGACGAAGCCCTACACAGGGACCTGGCCAACTTCAGGATCCAACACCCTCAGGTGACCCTCCTCCAGTACGTGGATGACCTGCTTCTGGCGGGAGCCACCAAACAGGACTGCTTAGAA(+TAGTGA)GGTACGAAGGCACTACTGCTGGAATTGTCTGACCTAGGCTACAGAGCCTCTGCTAAGAAGGCCCAGATTTGCAGGAGAGA NGG 10 8 17 8 24 0 100 5 10
    250610170617_tni7cthg CGACTGCCCCAAGGGTTCAAGAACTCCCCGACCATCTTTGACGAAGCCCTACACAGGGACCTGGCCAACTTCAGGATCCAACACCCTCAGGTGACCCTCCTCCAGTACGTGGATGACCTGCTT(+TAGTGA)CTGGCGGGAGCCACCAAACAGGACTGCTTAGAAGGTACGAAGGCACTACTGCTGGAATTGTCTGACCTAGGCTACAGAGCCTCTGCTAAGAAGGCCCAGATTTGCAGGAGAGA NGG 10 8 17 8 24 0 100 5 10
    250610170617_tni7ccn4 CGACTGCCCCAAGGGTTCAAGAACTCCCCGACCATCTTTGACGAAGCCCTACACAGGGACCTGGCCAACTTCAGGATCCAACACCCTCAGGTGACCCTCCTC(+TAGTGA)CAGTACGTGGATGACCTGCTTCTGGCGGGAGCCACCAAACAGGACTGCTTAGAAGGTACGAAGGCACTACTGCTGGAATTGTCTGACCTAGGCTACAGAGCCTCTGCTAAGAAGGCCCAGATTTGCAGGAGAGA NGG 10 8 17 8 24 0 100 5 10
    250610170617_tni7bcam CGACTGCCCCAAGGGTTCAAGAACTCCCCGACCATCTTTGACGAAGCCCTACACAGGGACCTGGCCAACTTCAGGATCCAACACCCTCAGGTGACCCTCCTCCAGTACGTGGATGACCTGCTTCTGGCGGGAGCCACCAAACAGGACTGCTTAGAAGGTACGAAGGCACTACTG(+TAGTGA)CTGGAATTGTCTGACCTAGGCTACAGAGCCTCTGCTAAGAAGGCCCAGATTTGCAGGAGAGA NGG 10 8 17 8 24 0 100 5 10
    250610170617_tni77z73 CGACTGCCCCAAGGGTTCAAGAACTCCCCGACCATCTTTGACGAAGCCCTACACAGGGACCTGGCCAACTTCAGGATCCAACACCCTCAGGTGACCCTCCTCCAGTACGTGGATGACCTGCTTCTGGCGGGAGCCACCAAACAGGACTGCTTAGAAGGTACGAAGGCA(+TAGTGA)CTACTGCTGGAATTGTCTGACCTAGGCTACAGAGCCTCTGCTAAGAAGGCCCAGATTTGCAGGAGAGA NGG 10 8 17 8 24 0 100 5 10
    250610170617_tni77vh8 CGACTGCCCCAAGGGTTCAAGAACTCCCCGACCATCTTTGACGAAGCCCTACACAGGGACCTGGCCAACTTCAGGATCCAACACCCTCAGGTGACCCTCCTCCAGTACGTGGATGACCTGCTTCTGGCGGGAGCCACCAAACAGGACTGCTTAGAAGGTACGAAGGCACTACTGCTGGAA(+TAGTGA)TTGTCTGACCTAGGCTACAGAGCCTCTGCTAAGAAGGCCCAGATTTGCAGGAGAGA NGG 10 8 17 8 24 0 100 5 10
    250610170617_tni76dwm CGACTGCCCCAAGGGTTCAAGAACTCCCCGACCATCTTTGACGAAGCCCTACACAGGGACCTGGCCAACTTCAGGATCCAACACCCTCAGGTGACCCTCCTCCAGTACGTG(+TAGTGA)GATGACCTGCTTCTGGCGGGAGCCACCAAACAGGACTGCTTAGAAGGTACGAAGGCACTACTGCTGGAATTGTCTGACCTAGGCTACAGAGCCTCTGCTAAGAAGGCCCAGATTTGCAGGAGAGA NGG 10 8 17 8 24 0 100 5 10