Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250908090318_jkfa6gmk GCTGCCACTCTCATCCCCGTCCCAACCATCTCTGTCAAAAATATACCTTTTTCATATGATATTCTGAGCTAATCTGATAAAATCTATGCCAATATATACTA(/G)TTGCTTGTGTACTAGAGAAGTACATTATTGCTGTACTCCTCTGTACATTACTGATCCCTGATGGTATATTTCTATCCTAACAGTGTCCCTTTGCAGATCAA NG 10 8 17 8 24 0 100 5 10
    250908090318_jkfa6bac TTCTAAAGTGCAATGCAAAAGGGACATCATGTATATGCAGCGTTTGTTTGGAATTTTCTTTGCTTTTGTTTTCTTTGCGGTTGTTCTGTGTGCATGGATTC(/T)CACACCTCTGCCGTAGGTAGATCCGTCAGCGGGCATTATTACCGTGTCTTGTAAAGGGTCGGTTTTGTTATGCAACATGCAGAATGCTGTTTTTAGCCTTG NG 10 8 17 8 24 0 100 5 10
    250908090318_jkfa6agz AGGTCGGGGGAGGGGAGCCCCCCTCGGGCTGTGGTTAGAGCGGGAGAGGAACTTCCCAGACTAGCTGGCACAGAGCCTCGGGAAGGCGGCGGGCACTGCAG(/A)GTGGTTTACGGGAAGTGCTGCAGCCTTGGGGTGGGGACAGCGTGGCCAGACCCACCGCCTCATCTGCACACCTGGGCTCAAGCGCTAATGACGACAGGGGA NG 10 8 17 8 24 0 100 5 10
    250908090318_jkfa5j45 ACTAACACATGGCGTTTGAAATAAGGCTGTAAAAAGATACAAACAAAACCAGCATTCAACTTGAACCAAGATTGTACATCTATGACAGTGATATAGATTTA(/T)AAAACCCTACTACCAAAACTGTAATTGATTTCATCATGCACTCTATAGAAAAAGACAATTATGGAAATTTCTCTTAGTTATGAATGTAACCAGCTCACTGG NG 10 8 17 8 24 0 100 5 10
    250908090318_jkfa51vl TGGAGTCAAAAGTGAGAAGCCTCAGTCATGTTCCTGATAAGAACGGAAGGTATTTGGGCAGACAGCCAAGCCCGCATTGACTGACACAGGGCCGTAACAGG(/C)AGAGATTTCTTCACAGACAGAACTGTTTTCCAAAGCAAGCTGCAGAGGCCACCGGTGGGGAGGGGGCACTGGGGACACTTTCCCTGGCCACTACCTTGTGA NG 10 8 17 8 24 0 100 5 10
    250908090318_jkfa4zk1 AAGTAAAGAGCTATAATCCCAACCTAAACTTTTCTGAGATTTTACAATAATTAGATCTTTTTCCAAGTTAATTGGGTTTTCCCTTCTCCCAGTCATAGGTG(/A)GTTTTTATCATCAAGACAGACTGATATTTTGTCAGGATATTTTCTTTTACAGTGTTTGATGTGCATAATGCCAGAGTTATTTTTTTATTATTCATTTTCTC NG 10 8 17 8 24 0 100 5 10
    250908090318_jkfa4vsc TGGGATTTAAACATCATTTTGAGAAGTGAAAAATCACCATGGGACAAGTTCCTCTGAGTATTCCAAGCTCCTTTGTAACAGCACCATCAAACATTTGCTAC(/T)GTTTTTCTTTAAAACTGAATGCCATTTGCACAGAACTGTACTTTTAAGGCTCTTACATGGTTTCCAGTCCACTGTTCAAAACTCAGAGATCTCAAAATCAT NG 10 8 17 8 24 0 100 5 10
    250908090318_jkfa4pup CAAAAGATATCCCTGTTTTCCTAATAATGTAAACAGTAAAACTGAGGGTTTCAGTTAAAGGGATAATTCGTAACAAAATCCCATTGTTGAAACAATGGTCA(/C)AACATAAACATCTTATAATTCAGATCTGCATTTGGTAACAGGAGAATTGAGAGTTTTGATGCTTTGCTGGTTTTCCCACAGTGGGAGATGTAGATACCATT NG 10 8 17 8 24 0 100 5 10
    250908090318_jkfa4cl1 AGTGAATTTACTCAATGCAATAGTTAAAGAGAAACTAGTTTCTACTAATACATTTTAATGGATATTTATGTATATACACACACATAGAGACAAACACATAT(/C)ATACACATACTCAGAGCAGGGACCGAGCCAGCTGTTGCTCTCCTTCTTTTCAGTTCTGTCATACTTGCTGGGAGATTCTATTCCACTTACAAATTAAGCCT NG 10 8 17 8 24 0 100 5 10
    250908090318_jkfa3x0l ATAAAAGCACAAAAGTAACAGCAACATAGATAATGCGAACACGTGTTGTTACACAAACTATAGTGTGAAACATATTAGTATTTAAAAAACAAAAAAAAATT(/C)GTTAATGGGAAAATCAATATTGTGGCCCACACACAAAAAGTCATTGCCAGATCAACTGGATAATGCTTGCTTGTTCAATAACTGAGAAAAAAATTTACCAT NG 10 8 17 8 24 0 100 5 10