Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250610170603_8lyw CGACTGCCCCAAGGGTTCAAGAACTCCCCGACCATCTTTGACGAAGCCCTACACAGGGACCTGGCCAACTTCAGGATCCAACACCCTCAGGTGACCCTCCTCCAGTACGTGGATGACCTGCTTCTGGCGGGAGCCACCAAACAGGACTGCTTAGAAGGTACGAAGGCACTACTGCTGGAATTGTCTGAC(+TAGTGA)CTAGGCTACAGAGCCTCTGCTAAGAAGGCCCAGATTTGCAGGAGAGA NGG 10 8 17 8 24 0 100 5 10
    250610170548_pw86 CGACTGCCCCAAGGGTTCAAGAACTCCCCGACCATCTTTGACGAA(+TAGTGA)GCCCTACACAGGGACCTGGCCAACTTCAGGATCCAACACCCTCAGGTGACCCTCCTCCAGTACGTGGATGACCTGCTTCTGGCGGGAGCCACCAAACAGGACTGCTTAGAAGGTACGAAGGCACTACTGCTGGAATTGTCTGACCTAGGCTACAGAGCCTCTGCTAAGAAGGCCCAGATTTGCAGGAGAGA NGG 10 8 17 8 24 0 100 5 10
    250610170512_6ouy CGACTGCCCCAAGGGTTCAAGAACTCCCCGACCATCTTTGACGAAGCCCTACAC(+TAGTGA)AGGGACCTGGCCAACTTCAGGATCCAACACCCTCAGGTGACCCTCCTCCAGTACGTGGATGACCTGCTTCTGGCGGGAGCCACCAAACAGGACTGCTTAGAAGGTACGAAGGCACTACTGCTGGAATTGTCTGACCTAGGCTACAGAGCCTCTGCTAAGAAGGCCCAGATTTGCAGGAGAGA NGG 10 8 17 8 24 0 100 5 10
    250610170458_6x5h CGACTGCCCCAAGGGTTCAAGAACTCCCCGACCATCTTTGACGAAGCCCTACACAGG(+TAGTGA)GACCTGGCCAACTTCAGGATCCAACACCCTCAGGTGACCCTCCTCCAGTACGTGGATGACCTGCTTCTGGCGGGAGCCACCAAACAGGACTGCTTAGAAGGTACGAAGGCACTACTGCTGGAATTGTCTGACCTAGGCTACAGAGCCTCTGCTAAGAAGGCCCAGATTTGCAGGAGAGA NGG 10 8 17 8 24 0 100 5 10
    250610170455_02yq CGACTGCCCCAAGGGTTCAAGAACTCCCCGACCATCTTTGACGAAGCCCTACACAGGGAC(+TAGTGA)CTGGCCAACTTCAGGATCCAACACCCTCAGGTGACCCTCCTCCAGTACGTGGATGACCTGCTTCTGGCGGGAGCCACCAAACAGGACTGCTTAGAAGGTACGAAGGCACTACTGCTGGAATTGTCTGACCTAGGCTACAGAGCCTCTGCTAAGAAGGCCCAGATTTGCAGGAGAGA NGG 10 8 17 8 24 0 100 5 10
    250610170426_s34j CGACTGCCCCAAGGGTTCAAGAACTCCCCGACCATCTTTGACGAAGCCCTACACAGGGAC(+TAGTGA)CTGGCCAACTTCAGGATCCAACACCCTCAGGTGACCCTCCTCCAGTACGTGGATGACCTGCTTCTGGCGGGAGCCACCAAACAGGACTGCTTAGAAGGTACGAAGGCACTACTGCTGGAATTGTCTGACCTAGGCTACAGAGCCTCTGCTAAGAAGGCCCAGATTTGCAGGAGAGA NGG 10 8 17 8 24 0 100 5 10
    250610163638_iql0y873 TGGAATACTCCCCTGCTACCGGTTAGGAAGCCTGGGACCAATGATTATCGACCAGTACAGGACTTGAGAGAGGTC(+TAGTGA)AATAAAAGGGTGCAGGACATACACCCAACGGTCCCGAACCCTTATAACCTCTTGAGCGCTCTCCCGCCCGAACGGAACTGGTACACAGTATTGGACTTAAAAGATGCCTTCTTCTGCCTGAGATTACACTCCCACTAGCCAACCACTTTTTGCCTTCGAATGGAGAGATCCAGGTACGGGAAGAACCGGGCAGCTCACCTGGACCCGACTGCCCCAA NGG 10 8 17 8 24 0 100 5 10
    250610163638_iql0xx0u TGGAATACTCCCCTGCTACCGGTTAGGAAGCCTGGGACCAATGATTATCGACCAGTACAGGACTTGAGAGAGGTCAATAAAAGGGTGCAGGACATACACCCAACGGTCCCGAACCCTTATAACCTCTTGAGCGCTCTCCCGCCCGAACGG(+TAGTGA)AACTGGTACACAGTATTGGACTTAAAAGATGCCTTCTTCTGCCTGAGATTACACTCCCACTAGCCAACCACTTTTTGCCTTCGAATGGAGAGATCCAGGTACGGGAAGAACCGGGCAGCTCACCTGGACCCGACTGCCCCAA NGG 10 8 17 8 24 0 100 5 10
    250610163638_iql0xq76 TGGAATACTCCCCTGCTACCGGTTAGGAAGCCTGGGACCAATGATTATCGACCAGTACAGGACTTGAGAGAGGTCAATAAAAGGGTGCAGGACATACACCCAACGGTCCCGAACCCT(+TAGTGA)TATAACCTCTTGAGCGCTCTCCCGCCCGAACGGAACTGGTACACAGTATTGGACTTAAAAGATGCCTTCTTCTGCCTGAGATTACACTCCCACTAGCCAACCACTTTTTGCCTTCGAATGGAGAGATCCAGGTACGGGAAGAACCGGGCAGCTCACCTGGACCCGACTGCCCCAA NGG 10 8 17 8 24 0 100 5 10
    250610163638_iql0xnts TGGAATACTCCCCTGCTACCGGTTAGGAAGCCTGGGACCAATGATTATCGACCAGTACAGGACTTGAGAGAGGTCAATAAAAGGGTGCAGGACATACACCCAACGGTCCCGAACCCTTATAACCTCTTGAGCGCTCTCCCGCCCGAACGGAACTGGTACACAGTATTGGACTTAAAAGATGCCTTCTTCTGCCTGAGATTACACTCCCACTAGCCAACCACTTTTTGCCTTCGAATGGAGAGATCCAGGTACGGGAAGAACCGG(+TAGTGA)GCAGCTCACCTGGACCCGACTGCCCCAA NGG 10 8 17 8 24 0 100 5 10