Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    260424130719_06w6 AAGTGTTGTTGGGCAGTCCCGTGTCATCCATG(+CGACTACATAGATC)GTACCTGCTTCAATTTCACACATAGCTGGTA NGG 10 8 17 8 24 0 100 5 10
    260424130648_9ykl CAGTTTACCAGCTATGTGTGAAATTGAAGCAG(+CGACTACATAGATC)GTACCATGGATGACACGGGACTGCCCAACAA NGG 10 8 17 8 24 0 100 5 10
    260424130630_o3tt ATTAATTAGGGTGGCTTGATCAGCTACAGAAG(+CGACTACATAGATC)GATCCTTTGGGGTCACATTCTTTTATCCTGT NGG 10 8 17 8 24 0 100 5 10
    260424130500_8ty2 GTGGGAAAGGAGGAGGGTCTGCTAACCTACAG(+CGACTACATAGATC)GTACCACAACCCTGAAAGGAGCGCCTTCTAG NGG 10 8 17 8 24 0 100 5 10
    260424130452_nbn3 GTGGGAAAGGAGGAGGGTCTGCTAACCTACAG(+CGACTACATAGATA)GTACCACAACCCTGAAAGGAGCGCCTTCTAG NGG 10 8 17 8 24 0 100 5 10
    260424130442_ja74 GTGGGAAAGGAGGAGGGTCTGCTAACCTACAG(+CGACTACATAGATT)GTACCACAACCCTGAAAGGAGCGCCTTCTAG NGG 10 8 17 8 24 0 100 5 10
    260424105359_dx4f GTGGGAAAGGAGGAGGGTCTGCTAACCTACAG(+CGACTACATAGATC)GTACCACAACCCTGAAAGGAGCGCCTTCTAG NGG 10 8 17 8 24 0 100 5 10
    260424104958_9lif ATCTTTTATATACTCTGCATAACAGACATGAG(+CGCGATGCGATGCA)GTACCAGTGAAGTTATGCAGACCAGTACCTG NGG 10 8 17 8 24 0 100 5 10
    260424104944_xco8 CTCTCCCACCCGGCTCTAGGCCAAAAGGCTCG(+CGCGATGCGATGCA)GTACCTCCTTACTGGGAGCCACGTAAATGGA NGG 10 8 17 8 24 0 100 5 10
    260424104930_etlf AAGGTTCCATTTACGTGGCTCCCAGTAAGGAG(+CGCGATGCGATGCA)GTACCGAGCCTTTTGGCCTAGAGCCGGGTGG NGG 10 8 17 8 24 0 100 5 10