Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250220110900_8u7b GACACGGGGCCGGGTTTGCCCACACCTGCAGTAGGCGTGGGCGACCGAGGAGGTAGCCCCCAGGAGGCTGCCAGGAGACCCCGAGGCTGCTCAGAGAGGCCAGATGCTGTGGACGTGGGGAATCATCGTGTCCTGGTCTATTTTCGGGCCTCTGCTGAGAGCGCAGGAATCCAGGCGAAGGGGCCCGAGGGCTGGGA(/G)CCACCTGTCAGATCCTTTCCCCAGCTGAAGGCAGGGTGTGGGTGATCCAGGGCCGGAAAAAGTCAAGGCCACCTCCAAGCCTTCCAATTTTAGAGCTGCACGTCTCCAGCTCCGGGACACGGAGTATCCTGCTGGGGCTCGAGAGGACCTATT NGG 10 8 17 8 24 0 100 5 10
    250219160042_lx3l GAGCGGATCAGGCCTCCCTCTGCTGGTCCAGAGGACAATAGCAAAGCAAATTCAGATGGTGAAACAGATTGGAAAAGGTCGCTATGGGGAAGTTTGGATGGGAAAGTGGCGTGGCGAAAAGGTAGCTGTGAAAGTGTTCTTCACTACAGAGGAGGCCAGCTGGTTCCGAGAGACAGAAATATATCA(/G)GACGGTGTTGATGAGGCATGAAAACATCTTGGGCTTCATTGCTGCAGATATCAAAGGGACGGGGTCCTGGACACAACTGTACCTAATCACAGATTATCATGAAAATGGTTCCCTCTATGATTACCTGAAGTCCACCACCCTAGACACTAAGTCGATGTTGAAGCTAGCCTATTCCGCAGTCAGTGGCCTCTGTCACTTACACACTGAAATCTTT NGG 10 8 17 8 24 0 100 5 10
    250219154945_evim agtttgagagccggctggcggatgcgctgcaggaactgT(/C)gggcccagcatgaggaccaggtggagcagtataagaaggagctggagaagacttattctgc NGG 10 8 17 8 24 0 100 5 100
    250219154857_emu9 agtttgagagccggctggcggatgcgctgcaggaactgT(/C)gggcccagcatgaggaccaggtggagcagtataagaaggagctggagaagacttattctgc NGG 10 8 17 8 24 0 100 5 10
    250219154736_b7nv agtttgagagccggctggcggatgcgctgcaggaactgT(/C)gggcccagcatgaggaccaggtggagcagtataagaaggagctggagaagacttattctgc NGG 10 8 17 8 24 0 100 5 10
    250219115806_4jq4 GACACGGGGCCGGGTTTGCCCACACCTGCAGTAGGCGTGGGCGACCGAGGAGGTAGCCCCCAGGAGGCTGCCAGGAGACCCCGAGGCTGCTCAGAGAGGCCAGATGCTGTGGACGTGGGGAATCATCGTGTCCTGGTCTATTTTCGGGCCTCTGCTGAGAGCGCAGGAATCCAGGCGAAGGGGCCCGAGGGCTGGGA(/G)CCACCTGTCAGATCCTTTCCCCAGCTGAAGGCAGGGTGTGGGTGATCCAGGGCCGGAAAAAGTCAAGGCCACCTCCAAGCCTTCCAATTTTAGAGCTGCACGTCTCCAGCTCCGGGACACGGAGTATCCTGCTGGGGCTCGAGAGGACCTATT NGG 10 8 17 8 24 0 100 5 10
    250218144344_3ba3 AATAGGTCCTCTCGAGCCCCAGCAGGATACTCCGTGTCCCGGAGCTGGAGACGTGCAGCTCTAAAATTGGAAGGCTTGGAGGTGGCCTTGACTTTTTCCGGCCCTGGATCACCCACACCCTGCCTTCAGCTGGGGAAAGGATCTGACAGGTGGT(/C)CCCAGCCCTCGGGCCCCTTCGCCTGGATTCCTGCGCTCTCAGCAGAGGCCCGAAAATAGACCAGGACACGATGATTCCCCACGTCCACAGCATCTGGCCTCTCTGAGCAGCCTCGGGGTCTCCTGGCAGCCTCCTGGGGGCTACCTCCTCGGTCGCCCACGCCTACTGCAGGTGTGGGCAAACCCGGCCCCGTGTC NGG 10 8 17 8 24 0 100 5 10
    250218144207_64ui CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250218144140_0hjx CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250218121340_rzbg GACACGGGGCCGGGTTTGCCCACACCTGCAGTAGGCGTGGGCGACCGAGGAGGTAGCCCCCAGGAGGCTGCCAGGAGACCCCGAGGCTGCTCAGAGAGGCCAGATGCTGTGGACGTGGGGAATCATCGTGTCCTGGTCTATTTTCGGGCCTCTGCTGAGAGCGCAGGAATCCAGGCGAAGGGGCCCGAGGGCTGGGA(/G)CCACCTGTCAGATCCTTTCCCCAGCTGAAGGCAGGGTGTGGGTGATCCAGGGCCGGAAAAAGTCAAGGCCACCTCCAAGCCTTCCAATTTTAGAGCTGCACGTCTCCAGCTCCGGGACACGGAGTATCCTGCTGGGGCTCGAGAGGACCTATT NGG 10 8 17 8 24 0 100 5 10