Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250213002936_f98c CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250211123344_mn2k AGAGCCCCGTGACCTTCTTTGACACGCCCCCCATGCTCTTTGTGTTCATCGCCCTGGGACG(/T)GTGGCTGGAACACGTGGCCAAGAGCAAAACTTCAGAAGCCCTTGCAAAACTCATGTCACT NGG 10 8 17 8 24 0 100 5 10
    250211120656_xd0h AAGTGATTATGGTCTAGTCTTTTGTCAATTTAGTTTTATATGGTTTTTCTTTTGACTTTCTTTGCATTTTTAAAGTTATATTTAATATTATGACAATATTTCATGAAAACAAATGTTGAATTCTGAAACCTCTATACTATCTATAAAAGTTTAATTTTCATAAGGAAAATTTGAAGTTATCCTCACACTACTTCTGATGAATGACCTTCTTCAGGACATGAAATTGTAACTTGTCAAAGTTTGAAGATAGCACTTGATCAAGTCTTGTGATATCAATAATTGTCTTTTAAGAACTTAAAATTTACACCTTATAAATAAAATGTTCCTGACTCTGAGTAACCCTTCCCTCTGTAGTATTTTTGAA(+ct)CAATGGCTGCGAAGGCACCGCCCTCTTTTGGAAGTTTACCCAGAAGCCAATGCACCTATCGGCCATAACAGAGACTCTTACATGGTTCCTTTCATACCGCTCTATAGAAATGGTGATTTCTTCATAACATCCAAGGATCTGGGATATGACTACAGCTACCTCCAAGAGTCAGGTAAAGCTCAGTTGCCTTCAGATGAACTATGGAATGCAATGTGGGTAACCTCTTTGGGGGCAGTTAAAAACACCTACTGTATACTCAATTTTTTTTCTAAAACTTCTAAAGAAACTATATTATGCCAATTTATAATTGTGTAAACTAGAACTGTATTTCAATGATTCCTAAATTTATAACTATTCCATCTAGTATAGGAATTTTATACAGTTAAATTGTTGAAGAATATAGGATTGGGAAGCTTTTGAACCATGAATTCCAATGAATTATGTCAGCTGTTTTATGACCTAGGGATATTCTTTCAATTCTGTGAATCATATTTTATTCATTATATATTTAGATAGGAATAACTGTATTTTTCTGAATCAGGTTAAAGAATTCAGTCTACGAATCATATGGTATTAACAATTTGTGATCTTCCATC NGG 10 8 17 8 24 0 100 5 10
    250210232817_61av CTTCCGCCTGCTGCTGGCCAGCCCCAGTGCCTGCTTTGAGCTGTTCGAACAGAAGCAGAAGGAAGGCTATGGGAACGTGACCCTGTTTGAAGACATTGGAGCAGAACAGCTCCTTTCT(-T)AATGGTAAGGTGACTCCCGCCTAGTGCCCTCCTTCCCCACCtcaagacagagtctcgctgtggaatcatggctgtcctggaacgc NGG 10 8 17 8 24 0 100 5 10
    250210232606_6vbv CTTCCGCCTGCTGCTGGCCAGCCCCAGTGCCTGCTTTGAGCTGTTCGAACAGAAGCAGAAGGAAGGCTATGGGAACGTGACCCTGTTTGAAGACATTGGAGCAGAACAGCTCCTTTCT(+T)AATGGTAAGGTGACTCCCGCCTAGTGCCCTCCTTCCCCACCtcaagacagagtctcgctgtggaatcatggctgtcctggaacgc NGG 10 8 17 8 24 0 100 5 10
    250210181328_ewli GACCCCTCACAATGAGCTGCCGTGGACTAGAGGATATAGCCTGAGGGCTGTCCCAGTTTCTCCCAATGTGGGAGCTCTCTTCCTGGCCTCTTGCGTCTCCCTTAAAGGAGGTAGGATTGAGCACAAGCTCGTTTCCAGTGCCTAACCTGGTATCTCCTCAGGTATGGAATAGAGTCCTGGAACTCCATCTCCAACTGTAGCCAGACCCTGTCCTATGACCTTACCGCAGTGACCTTGGACCTGTACCACAGCAATGGCTACCGGGCCAGAGTGT(/C)GGGCTGTGGACGGCAGCCGGCACTCCAACTGGACCGTCACCAACACCCGCTTCTCTGTGGATGAAGGTGCTTTTCCTCCCTTGACTTAGAACATGGCTCTGAAGTCCCTTCCAGCCAGGAACTCTAGTCTAGAGCTTTTCTGTCTATTACCATAGCTCACCATGTCTGCCAGCCTCCCTGGCCGGAGAACTAGTTGCCCAAACAGGGCAGG NGG 10 8 17 8 24 0 100 5 10
    250210164809_why3 gtcttgggcaaacctcctgtgcagtgtcttcatctctgggattaccaggcctcagtaacataccaaagtgaagaccagaagcgctctctgcctgccccccgcccccctgtgatgtgtaaccgaggccgcatacttcctgggtgggctgtggcctgcagttcctttcctgtgttcaggccctctctgctcccttgctctagGGACCGCCTGACTCACAATGACATCGTGGCTACCACCTACCTGAGTATGTCGAAAATCTCTGCCCC(+T)GGAGGAGAAATAGAAGgtatgttccctcttcgttctgccctttgaccccctgtgctctcccccgtaccccctctatccagcttacacttctagttttgagagtttgcagaaaaggggagattataaggcctctgttgcccattttccactgagaagagctctggcaatgaaagagatgtcttacagacacttagggttctccacacttggattgtgg NGG 10 8 17 8 24 0 100 5 10
    250210143407_27pq actttaactgcagttccaaagcttctctaacccaaaaattacagtaccaaatgattgactcaggattgtttttcccatattgatatgctggcaatata(+ggtttgtctggtcaaccaccgcggactcagtggtgtacggtacaaacc)ggatggtatgtaatggacagaactgatgcagatggttgaatgcgcttgtactatatgctaaaatatggaaaaggaaaaaaaaatctcacaagttcttttggaacttgtttcaagccaaaaactctcaagaaagcaaattgcacctcagctggattgatttccaaatgctagcatgtactgtatgggaggat NGG 10 8 17 8 100 0 200 5 10
    250210112933_rcsy CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250210112902_94zt CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10