Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    260424104652_0nr0 GACTTTATGTGGGAGCTGGGGATTTGAACTAG(+CGCGATGCGATGCA)GATCCTTGTATAGCAAGCATCCATATTCTGA NGG 10 8 17 8 24 0 100 5 10
    260424104639_loyo ATGGTTCAGAATATGGATGCTTGCTATACAAG(+CGCGATGCGATGCA)GATCCTAGTTCAAATCCCCAGCTCCCACATA NGG 10 8 17 8 24 0 100 5 10
    260424104620_sp9m GACCCACAGGAACTATGAAGGAAGAAGCGTAG(+CGCGATGCGATGCA)GATCCCAGAGCAATCATATAACCGAACTCTG NGG 10 8 17 8 24 0 100 5 10
    260424104607_wt53 GTAGCCTTTGCGATTGTGTGATCCAAGAGCTG(+CGCGATGCGATGCA)GATCCATACAATTTGTGCCACCCTGAAATTG NGG 10 8 17 8 24 0 100 5 10
    260424104553_ys4g AACTGGGGTACACTTTCCAGAACTGCCTTGAG(+CGCGATGCGATGCA)GTACCTAAAACTTCCACCGAATGAACCTTGG NGG 10 8 17 8 24 0 100 5 10
    260424104539_r0dp TGGTAAGGATGTGGTTCTGTGTAGAAAACGTG(+CGCGATGCGATGCA)GTACCAAGTGAGAACAGCATTTCATGTCCTG NGG 10 8 17 8 24 0 100 5 10
    260424104522_hy64 GTATGCGTTCATGGTGGAACACAACCACATCG(+CGCGATGCGATGCA)GTACCTACTTCAGAACAATTATCTGAGAAAA NGG 10 8 17 8 24 0 100 5 10
    260424104442_au3u TCAGTCTCAGCACTCAGAGACAGAGCTAGGTG(+CGCGATGCGATGCA)GATCCCTGAGTTCGAGGCCAGCCTGGTCTAC NGG 10 8 17 8 24 0 100 5 10
    260424104430_1ght TCTCTGTAGACCAGGCTGGCCTCGAACTCAGG(+CGCGATGCGATGCA)GATCCACCTAGCTCTGTCTCTGAGTGCTGAG NGG 10 8 17 8 24 0 100 5 10
    260424104418_1zfi AGACTCGGCCAGAGACAGACGTTAGAGGACGG(+CGCGATGCGATGCA)GATCCAGTCCCTCTCCCACAGCAGGCAAAGC NGG 10 8 17 8 24 0 100 5 10