Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250906012959_qimz TGTGACCAGCCCTGCAGGTGACTTGTTTGTGTCCCCAGATTACACTGACGACCATCG(/A)GCTATGGTGACAAGACGCCACAC NGG 10 8 17 8 24 0 100 5 10
    250829091546_es7z AATCTTGTGTGAGACATGTTCTAATTTAGTTGTATTTTATTATTTTTATTGTAAGGCCTGCTGAAAATGACTGAGTATAAACTTGTGGTGGTTGGAGCTG(/A)GtGGCGTAGGCAAGAGCGCCTTGACGATACAGCTAATTCAGAATCACTTTGTGGATGAGTATGACCCTACGATAGAGGTAACGCTGCTCTACAGTCTGCG NGG 10 8 17 8 24 0 100 5 10
    250829024702_xapa gttcctctctaactttctgtttttggtaataggacatctccaagtttgcagagaaggacaatatcgtccttggagaaggtggaatcacactgagtggTgA(/g)Tcagcgagcaagaatttccttagcaaggtgaatatcccattattggttcagcgagcatttgttgtaaatatcatgcatgtaaaaattatagacatttctctg NG 10 8 17 8 24 0 100 5 100
    250829020730_cx86 gttcctctctaactttctgtttttggtaataggacatctccaagtttgcagagaaggacaatatcgtccttggagaaggtggaatcacactgagtggTgA(/g)Tcagcgagcaagaatttccttagcaaggtgaatatcccattattggttcagcgagcatttgttgtaaatatcatgcatgtaaaaattatagacatttctctg NG 10 8 17 8 24 0 100 5 10
    250829012434_efvc tatatgactgcacttgaaggaaaatgtacctttcaagttcagaaacacatgaacagttcctctctaactttctgtttttggtaataggacatctccaagtttgcagagaaggacaatatcgtccttggagaaggtggaatcacactgagtggTgA(/g)Tcagcgagcaagaatttccttagcaaggtgaatatcccattattggttcagcgagcatttgttgtaaatatcatgcatgtaaaaattatagacatttctctgttttttatattctgtttctgaaa NGG 10 8 17 8 24 0 100 5 50
    250829012256_pilt tatatgactgcacttgaaggaaaatgtacctttcaagttcagaaacacatgaacagttcctctctaactttctgtttttggtaataggacatctccaagtttgcagagaaggacaatatcgtccttggagaaggtggaatcacactgagtggTgA(/g)Tcagcgagcaagaatttccttagcaaggtgaatatcccattattggttcagcgagcatttgttgtaaatatcatgcatgtaaaaattatagacatttctctgttttttatattctgtttctgaaa NGG 10 8 17 8 24 0 100 5 10
    250827211518_vcz9 CTTCACGGTAACTGGGAGAGGGAGCCCCTCCAGGT(/A)GAGAGCAGAAACCACCTAAGAAGCCCAAATCTCCCAAAGCTCCAGGAACTGGCAGAGGCCGGGGAC NGG 10 8 17 8 24 0 100 5 10
    250826125222_zbfn CTCTAGGAGGACCCAGCCTGGCCCAGGGGGTGAGGGGTGTAATGGTTACCTGTGCCCGTGTGATTAGCCTGGATGGGCCCCATACTCAGCTCCATCCGTCG(/A)CCCACTGCGTGTACTCTCATAGCGGCTGAAGGTACCAAGGGCAGGAAAGGTCGGGGGGTCAAAGGAGTCACAGTATGTGGCATTGCAGACACACACCACC NGG 10 8 17 8 24 0 100 5 10
    250826120941_2953 CTCTAGGAGGACCCAGCCTGGCCCAGGGGGTGAGGGGTGTAATGGTTACCTGTGCCCGTGTGATTAGCCTGGATGGGCCCCATACTCAGCTCCATCCGTCG(/A)CCCACTGCGTGTACTCTCATAGCGGCTGAAGGTACCAAGGGCAGGAAAGGTCGGGGGGTCAAAGGAGTCACAGTATGTGGCATTGCAGACACACACCACC NGG 10 8 17 8 24 0 100 5 10
    250826120909_7c7o CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10