Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250213151557_9l0o TAAAATGTTCCTGACTCTGAGTAACCCTTCCCTCTGTAGTATTTTTGAACAATGGCTGCGAAGGCACCGCCCTCTTTTGGAAGTTTACCCAGAAGCCAATGCACCTATCGGCCATAACAGAGACTCTT(+ctt)ACATGGTTCCTTTCATACCGCTCTATAGAAATGGTGATTTCTTCATAACATCCAAGGATCTGGGATATGACTACAGCTACCTCCAAGAGTCAGGTAAAGCTCAGTTGCCTTCAGATGAACTATGGAATGCAATG NGG 10 8 17 8 24 0 70 5 10
    250213145154_c44l AAGTGATTATGGTCTAGTCTTTTGTCAATTTAGTTTTATATGGTTTTTCTTTTGACTTTCTTTGCATTTTTAAAGTTATATTTAATATTATGACAATATTTCATGAAAACAAATGTTGAATTCTGAAACCTCTATACTATCTATAAAAGTTTAATTTTCATAAGGAAAATTTGAAGTTATCCTCACACTACTTCTGATGAATGACCTTCTTCAGGACATGAAATTGTAACTTGTCAAAGTTTGAAGATAGCACTTGATCAAGTCTTGTGATATCAATAATTGTCTTTTAAGAACTTAAAATTTACACCTTATAAATAAAATGTTCCTGACTCTGAGTAACCCTTCCCTCTGTAGTATTTTTGAACAATGGCTGCGAAGGCACCGCCCTCTTTTGGAAGTTTACCCAGAAGCCAATGCACCTATCGGCCATAACAGAGACTCTT(+ctt)ACATGGTTCCTTTCATACCGCTCTATAGAAATGGTGATTTCTTCATAACATCCAAGGATCTGGGATATGACTACAGCTACCTCCAAGAGTCAGGTAAAGCTCAGTTGCCTTCAGATGAACTATGGAATGCAATGTGGGTAACCTCTTTGGGGGCAGTTAAAAACACCTACTGTATACTCAATTTTTTTTCTAAAACTTCTAAAGAAACTATATTATGCCAATTTATAATTGTGTAAACTAGAACTGTATTTCAATGATTCCTAAATTTATAACTATTCCATCTAGTATAGGAATTTTATACAGTTAAATTGTTGAAGAATATAGGATTGGGAAGCTTTTGAACCATGAATTCCAATG NGG 10 8 17 8 24 0 100 5 10
    250213145044_r9wq AAGTGATTATGGTCTAGTCTTTTGTCAATTTAGTTTTATATGGTTTTTCTTTTGACTTTCTTTGCATTTTTAAAGTTATATTTAATATTATGACAATATTTCATGAAAACAAATGTTGAATTCTGAAACCTCTATACTATCTATAAAAGTTTAATTTTCATAAGGAAAATTTGAAGTTATCCTCACACTACTTCTGATGAATGACCTTCTTCAGGACATGAAATTGTAACTTGTCAAAGTTTGAAGATAGCACTTGATCAAGTCTTGTGATATCAATAATTGTCTTTTAAGAACTTAAAATTTACACCTTATAAATAAAATGTTCCTGACTCTGAGTAACCCTTCCCTCTGTAGTATTTTTGAA(+ctt)CAATGGCTGCGAAGGCACCGCCCTCTTTTGGAAGTTTACCCAGAAGCCAATGCACCTATCGGCCATAACAGAGACTCTTACATGGTTCCTTTCATACCGCTCTATAGAAATGGTGATTTCTTCATAACATCCAAGGATCTGGGATATGACTACAGCTACCTCCAAGAGTCAGGTAAAGCTCAGTTGCCTTCAGATGAACTATGGAATGCAATGTGGGTAACCTCTTTGGGGGCAGTTAAAAACACCTACTGTATACTCAATTTTTTTTCTAAAACTTCTAAAGAAACTATATTATGCCAATTTATAATTGTGTAAACTAGAACTGTATTTCAATGATTCCTAAATTTATAACTATTCCATCTAGTATAGGAATTTTATACAGTTAAATTGTTGAAGAATATAGGATTGGGAAGCTTTTGAACCATGAATTCCAATG NGG 10 8 17 8 24 0 100 5 10
    250213144931_cd03 ACTGATAATAAATTCAAGTTTCTTTTACTTCTCAATAGCTCGTAAGCTGAAGAAACTTGGAAATCTAAAACTACAGGAGGAAGGAGAAAACTCCAATGCTGGCAGCCCCACTGAGGACCCATCCCAGAAGATGACTGTATCACACATTGAAGGCTATGAATGTCAGCCTATCTTTCTTAACGTCCTGGAAGCCATTGAGCCAGGAGTGGTGTGTGCCGGACATGACAACAACCAACCAGATTCCTTTGCTGCCTTGTTATCTAGCCTCAATGA(+ctt)GCTTGGAGAGAGGCAGCTTGTGCATGTGGTCAAGTGGGCCAAGGCCTTGCCTGGTAAGGAAAAGAAGAATGAGGATGTGGGATGCAGACAGGATATCATAATTATTGAATGGTTCTTTGGGCCAGGCACTCGTTACTTCATGCAGCCCTCGATGCCACACTGTGGAGAAGTGATTTTCGTATAAAAAATTCTCACTGTAAACATTGA NGG 10 8 17 8 24 0 100 5 10
    250213122015_v888 TAAGGAAAATTTGAAGTTATCCTCACACTACTTCTGATGAATGACCTTCTTCAGGACATGAAATTGTAACTTGTCAAAGTTTGAAGATAGCACTTGATCAAGTCTTGTGATATCAATAATTGTCTTTTAAGAACTTAAAATTTACACCTTATAAATAAAATGTTCCTGACTCTGAGTAACCCTTCCCTCTGTAGTATTTTTGAACAATGGCTGCGAAGGCACCGCCCTCTTTTGGAAGTTTACCCAGAAGCCAATGCACCTATCGGCCATAACAGAGACTCTT(+at)ACATGGTTCCTTTCATACCGCTCTATAGAAATGGTGATTTCTTCATAACATCCAAGGATCTGGGATATGACTACAGCTACCTCCAAGAGTCAGGTAAAGCTCAGTTGCCTTCAGATGAACTATGGAATGCAATGTGGGTAACCTCTTTGGGGGCAGTTAAAAACACCTACTGTATACTCAATTTTTTTTCTAAAACT NGG 10 8 17 8 24 0 100 5 10
    250213122000_505p TAAGGAAAATTTGAAGTTATCCTCACACTACTTCTGATGAATGACCTTCTTCAGGACATGAAATTGTAACTTGTCAAAGTTTGAAGATAGCACTTGATCAAGTCTTGTGATATCAATAATTGTCTTTTAAGAACTTAAAATTTACACCTTATAAATAAAATGTTCCTGACTCTGAGTAACCCTTCCCTCTGTAGTATTTTTGAA(+t)CAATGGCTGCGAAGGCACCGCCCTCTTTTGGAAGTTTACCCAGAAGCCAATGCACCTATCGGCCATAACAGAGACTCTTACATGGTTCCTTTCATACCGCTCTATAGAAATGGTGATTTCTTCATAACATCCAAGGATCTGGGATATGACTACAGCTACCTCCAAGAGTCAGGTAAAGCTCAGTTGCCTTCAGATGAACTATGGAATGCAATGTGGGTAACCTCTTTGGGGGCAGTTAAAAACACCTACTGTATACTCAATTTTTTTTCTAAAACT NGG 10 8 17 8 24 0 100 5 10
    250213121919_d3ev TAAGGAAAATTTGAAGTTATCCTCACACTACTTCTGATGAATGACCTTCTTCAGGACATGAAATTGTAACTTGTCAAAGTTTGAAGATAGCACTTGATCAAGTCTTGTGATATCAATAATTGTCTTTTAAGAACTTAAAATTTACACCTTATAAATAAAATGTTCCTGACTCTGAGTAACCCTTCCCTCTGTAGTATTTTTGAA(+ctt)CAATGGCTGCGAAGGCACCGCCCTCTTTTGGAAGTTTACCCAGAAGCCAATGCACCTATCGGCCATAACAGAGACTCTTACATGGTTCCTTTCATACCGCTCTATAGAAATGGTGATTTCTTCATAACATCCAAGGATCTGGGATATGACTACAGCTACCTCCAAGAGTCAGGTAAAGCTCAGTTGCCTTCAGATGAACTATGGAATGCAATGTGGGTAACCTCTTTGGGGGCAGTTAAAAACACCTACTGTATACTCAATTTTTTTTCTAAAACTTCTAAAGAAACTATATTATGCCAATTTATAATTGTGTAAACTAGAACTGTATTTCAATGATTCCTAAATTTATAACTATTCCATCTAGTATAGGAATTTTATACAGTTAAATTGTTGAAGAATATAGGATTGGGAAGCTTTTGAACCATGAATTCCAATG NGG 10 8 17 8 24 0 100 5 10
    250213121622_zp0n GAAGGCTGACAGGAGGGGCTGTGGCGTGTAGATTAGGGTCGTGTGCACACATGGGGTCTGCTATTGGGAGTCTCTGCTTTACTTAAGGCCCATGAGACCCACCTTGCTCTGGGGTCAGAGTGGGTGCCCTGTACCACCTCTTGTAACTTTTCACCTTGCTCTTGCAGTTCCGGTTTGGAGGCGGTGGCGATGCCGGTTCTAAGGGCCCCATGGTCTCTGCGCAGGAGTCCCAGGCCCAGGCTATCCT(+ctt)CCAGCAAGCCAGGGTAAGTAATGTGGACCCCTAGGGACTCATGTGACAGAGCTTGGCCATGGTGGCTGCACAGAGTGAATGATTGTCCTACAGTTGTCTGGTCAGGAACCTAGGGAAGGTTGGGTCTTCCATGTGCAGCTGGAGAATGTGGGGGAACTTACAGAGAGCTTTCAGGGCTATCCTCTCCACAGTTAGGGATAAGAGGTCTCTGGGCTTGGGTTGGGATTGAGTGC NGG 10 8 17 8 24 0 100 5 10
    250213121549_5l5k GAAGGCTGACAGGAGGGGCTGTGGCGTGTAGATTAGGGTCGTGTGCACACATGGGGTCTGCTATTGGGAGTCTCTGCTTTACTTAAGGCCCATGAGACCCACCTTGCTCTGGGGTCAGAGTGGGTGCCCTGTACCACCTCTTGTAACTTTTCACCTTGCTCTTGCAGTTCCGGTTTGGAGGCGGTGGCGATGCCGGTTCTAAGGGCCCCATGGTCTCTGCGCAGGAGTCCCAGGCCCAGGCTATCCTCCA(+ctt)GCAAGCCAGGGTAAGTAATGTGGACCCCTAGGGACTCATGTGACAGAGCTTGGCCATGGTGGCTGCACAGAGTGAATGATTGTCCTACAGTTGTCTGGTCAGGAACCTAGGGAAGGTTGGGTCTTCCATGTGCAGCTGGAGAATGTGGGGGAACTTACAGAGAGCTTTCAGGGCTATCCTCTCCACAGTTAGGGATAAGAGGTCTCTGGGCTTGGGTTGGGATTGAGTGC NGG 10 8 17 8 24 0 100 5 10
    250213121408_465q CCGAGCTTCCTATGACTCCCTGAAGTTGTCGTGCTCCCCTAGCGTCCGCCACCCGGGACGTGTCTCTGGTCCCCGTGCATCGTGTGCGTCTCCCGCTCCCCGGCCCGCGCACCCCGCCGGCATGGACGTACACACCCGCTGGAAAGCTGCGCGCCCAGGCGCCCTGCTGCTGTCTTCGCCGCTACTCCTGTTCCTGCTGCTGCTGTGGGCGCCGCCTTCG(+gt)AGCCGCGCAGGTAAGAGTGCTCTGGGGACCGGACCTCGGGGACTGTAAGAGGTGCGCAGAACCTCCGCGCCGCTGTCATCTCGGGCTCCTTCGCCGCCGGCCTCCTCCGAGCCTGCAGAACTTTCCTCCTTGGCCTGTGGAATGCACCGGGCAAGACAACGAATACCATTCGTTGGGGGCCCAGGAGAGGGAGCCGGGCCAACTCCATCCCCGCGGCGTACCGCCCCCTCCTCAAACGGGCCTGCTGGGTGGGGCTGTTG NGG 10 8 17 8 24 0 100 5 10