Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250126172331_l3ei tgatattttttcttgaaacaaaacttttcatggtgggcgtgaataaccatttcacaaagtgcaaggg(+ATT)tagttttaaacttttttatcgttttttttttcccctcttggagtaataatttgttcggtatacttcagttcatac NGG 10 8 17 8 24 0 100 5 10
    250126172308_4x9g tgatattttttcttgaaacaaaacttttcatggtgggcgtgaataaccatttcacaaagtgcaaggg(+A)tagttttaaacttttttatcgttttttttttcccctcttggagtaataatttgttcggtatacttcagttcatac NGG 10 8 17 8 24 0 100 5 10
    250126081825_yvrz GTCTTGGGCAAACCTCCTGTGCAGTGTCTTCATCTCTGGGATTACCAGGCCTCAGTAACATACCAAAGTGAAGACCAGAAGCGCTCTCTGCCTGCCCCCCGCCCCCCTGTGATGTGTAACCGAGGCCGCATACTTCCTGGGTGGGCTGTGGCCTGCAGTTCCTTTCCTGTGTTCAGGCCCTCTCTGCTCCCTTGCTCTAGGGACCGCCTGACTCACAATGACATCGTGGCTACCACCTACCTGAGTATGTCGAAAATCTCTGCCCC(/T)GGAGGAGAAATAGAAGGTATGTTCCCTCTTCGTTCTGCCCTTTGACCCCCTGTGCTCTCCCCCGTACCCCCTCTATCCAGCTTACACTTCTAGTTTTGAGAGTTTGCAGAAAAGGGGAGATTATAAGGCCTCTGTTGCCCATTTTCCACTGAGAAGAGCTCTGGCAATGAAAGAGATGTCTTACAGACACTTAGGGTTCTCCACACTTGGATTGTGG NGG 10 8 17 8 24 0 100 5 10
    250126081808_mhwa GTCTTGGGCAAACCTCCTGTGCAGTGTCTTCATCTCTGGGATTACCAGGCCTCAGTAACATACCAAAGTGAAGACCAGAAGCGCTCTCTGCCTGCCCCCCGCCCCCCTGTGATGTGTAACCGAGGCCGCATACTTCCTGGGTGGGCTGTGGCCTGCAGTTCCTTTCCTGTGTTCAGGCCCTCTCTGCTCCCTTGCTCTAGGGACCGCCTGACTCACAATGACATCGTGGCTACCACCTACCTGAGTATGTCGAAAATCTCTGCCCC(/T)GGAGGAGAAATAGAAGGTATGTTCCCTCTTCGTTCTGCCCTTTGACCCCCTGTGCTCTCCCCCGTACCCCCTCTATCCAGCTTACACTTCTAGTTTTGAGAGTTTGCAGAAAAGGGGAGATTATAAGGCCTCTGTTGCCCATTTTCCACTGAGAAGAGCTCTGGCAATGAAAGAGATGTCTTACAGACACTTAGGGTTCTCCACACTTGGATTGTGG NGG 10 8 17 8 24 0 100 5 10
    250125040125_ybfa CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250124173143_1030 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250124013431_iqge accctctctctctctgtctttctctcaccctctctcttcagggtgaccggga(/g)ctttccagggcccctgggtgaggctggagagaaggtaagtgcaacctggggggtgccaagggccctggag NGG 10 8 17 8 24 0 100 5 10
    250123225314_9f1s ATGGCGCACGCTGGGAGAACAGGGTACGATAACCGGGAGATAGTGATGAAGTACATCCATTATAAGCTGTCGCAGAGGGGCTACGAGTGGGATGCGGGAGATGTGGGCGCCGCGCCCCCGGGGGCCGCCCCCGCACCGGGCATCTTCTCCTCCCAGCCCGGGCACACGCCCCATCCAGCCGCATCCCGGGACCCGGTCGCCAGGACCTCGCCGCTGCAGACCCCGGCTGCCCCCGGCGCCGCCGCGGGGCCTGCGCTCAGCCCGGTGCCACCTGTGGTCCACCTGACCCTCCGCCAGGCCGG(/T)CGACGACTTCTCCCGCCGCTACCGCCGCGACTTCGCCGAGATGTCCAGCCAGCTGCACCTGACGCCCTTCACCGCGCGGGGACGCTTTGCCACGGTGGTGGAGGAGCTCTTCAGGGACGGGGTGAACTGGGGGAGGATTGTGGCCTTCTTTGAGTTCGGTGGGGTCATGTGTGTGGAGAGCGTCAACCGGGAGATGTCGCCCCTGGTGGACAACATCGCCCTGTGGATGACTGAGTACCTGAACCGGCACCTGCACACCTGGATCCAGGATAACGGAGGCTGGgtaggtgcacttggtgatgtgagtctgggctgaggccacaggtccgagatgcgggggttggagtgcgggtgggctcctggggcaatgggaggctgtggagccggcgaaataaaatcagagttgttgcttcccggcgtccctacctcctcctctggacaa NGG 10 8 17 8 24 0 100 5 10
    250122000206_0tnb ggggtgctcccgggcccccggaccgagccagggacgagcctgcccgcggcggcagccgggccgcggcttcgcctaggctcG(/A)cagcgcgggagcgcgtggggcgcggcggcggcggggagt NGG 10 8 17 8 24 0 100 5 10
    250121184149_p2e1 AGCCGGTGCCTATCTACCAGATTACTAGTAGTATATATGTTTTTTTTTTCATCTTTTTCTTCCCTTTTTGTTTGGATTAG(/A)ACAGGGATATGCCCCCTGCCCCGGGTTGTCGTCGTCACCACTGTGCAGAG NGG 10 8 17 8 24 0 100 5 10