Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    260424102337_a04a AGCCAGAGTCAAACTGATCCTTTATACAGGGG(+CGTATC)GTACCATGACAGTACAACAGTGTCGTTAACC NGG 10 8 17 8 24 0 100 5 10
    260424101915_gkuz GCAGTACTCTGAAGCAGGCAAATGGGTCTTAG(+CGTATC)GATCCCTCCCAGAAACCTGCTCTGACCCAGA NGG 10 8 17 8 24 0 100 5 10
    260424101901_qnp0 GTTAGGAGAGGTTAGGAAACCTCTAGAAAGTG(+CGTATC)GATCCTTGCTGGGGGTAATTATATCACTGGG NGG 10 8 17 8 24 0 100 5 10
    260424101846_56ac CCTACCCCAGTGATATAATTACCCCCAGCAAG(+CGTATC)GATCCACTTTCTAGAGGTTTCCTAACCTCTC NGG 10 8 17 8 24 0 100 5 10
    260424101831_afuy GCTACCCAGAGGCCTTTCGTCTCCCTGTTCTG(+CGTATC)GTACCCAGCTTCCCAATCAGCAGCCAGAGCT NGG 10 8 17 8 24 0 100 5 10
    260424101754_tgwa TTGTTCTAGAAGGCGCTCCTTTCAGGGTTGTG(+CGTATC)GTACCTGTAGGTTAGCAGACCCTCCTCCTTT NGG 10 8 17 8 24 0 100 5 10
    260424101733_v0s5 GTGGGAAAGGAGGAGGGTCTGCTAACCTACAG(+CGTATC)GTACCACAACCCTGAAAGGAGCGCCTTCTAG NGG 10 8 17 8 24 0 100 5 10
    260424101717_ewn7 GTGTAGTGTTCCTAGATTCAGCTGAAGACCTG(+CGTATC)GTACCTAGGGTTCCTAACGGGATGGTGTTAG NGG 10 8 17 8 24 0 100 5 10
    260424101700_agyl CAAAACCATCTGTAGCTCCTCCACTTTCAGGG(+CGTATC)GATCCGGCACCCTCTGCAGGCACTAGGAGCA NGG 10 8 17 8 24 0 100 5 10
    260424101639_l4t0 ACTTCTGCTCCTAGTGCCTGCAGAGGGTGCCG(+CGTATC)GATCCCCTGAAAGTGGAGGAGCTACAGATGG NGG 10 8 17 8 24 0 100 5 10