Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250610162330_i1am8bs3 TTCAGTGCTGCTACAACCATTAGGAAAACTAAAAGAAAAAAAATCCTGGGTGATGGGTGCCACAGGGCAACGGCAGTATCCATGGACTACCCGAAGAACCGTT(+TAGTGA)GACTTGGGAGTGGGACGGGTAACCCACTCGTTTCTG NGG 10 8 17 8 24 0 100 5 10
    250610162330_i1am3x4m TTCAGTGCTGCTACAACCATTAGGAAAACTAAAAGAAAAAAAATCCTGGGTGATGGGTGCCACAGGGCAACGGCAGTATCCATGGACTACCCGAAGAACC(+TAGTGA)GTTGACTTGGGAGTGGGACGGGTAACCCACTCGTTTCTG NGG 10 8 17 8 24 0 100 5 10
    250610162300_biz6q748 TTCAGTGCTGCTACAACCATTAGGAAAACTAAAAGAAAAAAAATCCTGGGTGATGGGTGCCACAGGGCAACGGCAGTATCCATGGACTACCCGAAGAACC(+TAGTGA)GTTGACTTGGGAGTGGGACGGGTAACCCACTCGTTTCTG NGG 10 8 17 8 24 0 100 5 10
    250610162300_biz659ap TTCAGTGCTGCTACAACCATTAGGAAAACTAAAAGAAAAAAAATCCTGGGTGATGGGTGCCACAGGGCAACGGCAGTATCCATGGACTACCCGAAGAACCGTT(+TAGTGA)GACTTGGGAGTGGGACGGGTAACCCACTCGTTTCTG NGG 10 8 17 8 24 0 100 5 10
    250610162218_tfl0t335 TTCAGTGCTGCTACAACCATTAGGAAAACTAAAAGAAAAAAAATCCTGGGTGATGGGTGCCACAGGGCAACGGCAGTATCCATGGACTACCCGAAGA(+TAGTGA)ACCGTTGACTTGGGAGTGGGACGGGTAACCCACTCGTTTCTG NGG 10 8 17 8 24 0 100 5 10
    250610162218_tfl0qyp5 TTCAGTGCTGCTACAACCATTAGGAAAACTAAAAGAAAAAAAATCCTGGGTGATGGGTGCCACAGGGCAACGGCAGTATCCATGGACTACCCGA(+TAGTGA)AGAACCGTTGACTTGGGAGTGGGACGGGTAACCCACTCGTTTCTG NGG 10 8 17 8 24 0 100 5 10
    250610162218_tfl0psl5 TTCAGTGCTGCTACAACCATTAGGAAAACTAAAAGAAAAAAAATCCTGGGTGATGGGTGCCACAGGGCAACGGCAGTATCCATGG(+TAGTGA)ACTACCCGAAGAACCGTTGACTTGGGAGTGGGACGGGTAACCCACTCGTTTCTG NGG 10 8 17 8 24 0 100 5 10
    250610162218_tfl0p2xp TTCAGTGCTGCTACAACCATTAGGAAAACTAAAAGAAAAAAAATCCTGGGTGATGGGTGCCACAGGGCAACGGCAGTATCCATGGACTACC(+TAGTGA)CGAAGAACCGTTGACTTGGGAGTGGGACGGGTAACCCACTCGTTTCTG NGG 10 8 17 8 24 0 100 5 10
    250610162218_tfl0lcd6 TTCAGTGCTGCTACAACCATTAGGAAAACTAAAAGAAAAAAAATCCTGGGTGATGGGTGCCACAGGGCAACGGCAGTATCCATGGACT(+TAGTGA)ACCCGAAGAACCGTTGACTTGGGAGTGGGACGGGTAACCCACTCGTTTCTG NGG 10 8 17 8 24 0 100 5 10
    250610162158_eanyy9pz TTCAGTGCTGCTACAACCATTAGGAAAACTAAAAGAAAAAAAATCCTGGGTGATGGGTGCCACAGGGCAACGGCAGTATCCATGGACTACCCGAAGAACC(+TAGTGA)GTTGACTTGGGAGTGGGACGGGTAACCCACTCGTTTCTG NGG 10 8 17 8 24 0 100 5 10