Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250721194727_rap9 TGGCTGCGACGTGGGGCCGGACGGGCGCCTCCTCCGCGGGTAC(/T)CACCAGGACGCCTACGACGGCAAGGATTACATCGCCCTGAACGAGGACCTGAGCTCCTGGA NGG 10 8 17 8 24 0 100 5 10
    250721192907_mc8i TGCGACGTGGGGCCGGACGGGCGCCTCCTCCGCGGGTACCACCAGG(/C)ACGCCTACGACGGCAAGGATTACATCGCC NGG 10 8 17 8 24 0 100 5 10
    250721121026_3nnd gacttcacctggatcctgtgtcttcccccaggccggctctgagtataccaccatccactacaagtacatgtgtaatagctcctgcatggggggcatgaac(-cgc)cgacctatccttaccatcatcacactggaagactccaggtaggaaggcgcgtggtaggttaggttagcctgtttcttccccagcttctgcctgtttctgt NGG 10 8 17 8 24 0 100 5 10
    250721120849_9zdq gacttcacctggatcctgtgtcttcccccaggccggctctgagtataccaccatccactacaagtacatgtgtaatagctcctgcatggggggcatgaaccgc(/g)cgacctatccttaccatcatcacactggaagactccaggtaggaaggcgcgtggtaggttaggttagcctgtttcttccccagcttctgcctgtttctgt NGG 10 8 17 8 24 0 100 5 10
    250718192054_a4y8 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250716163356_g4bs aggcaaaatcccctattttctggccaatggacccaaatcttccacatgg(-C)cctttcccgcagccccccagcctatacagccccagatattcttaccacaacatt NGG 10 8 17 8 24 0 100 5 10
    250714011124_b6c7 GTAGCTGTGCGCCTGGCTACAAGCTGGGGGACGACCTCCTGCAGTGTCACCCCGCAGTGAAGtTCCCTTGTGGGAGGCCCTGGAAGCGGATGGAGAAG(+AAG)CGCAGTCACCTGAAACGAGACACAGAAGACCAAGAAGACCAAGTAGATCCGCGGCTCATTGATG NGG 15 8 20 8 30 0 100 5 10
    250714004451_4ges GTAGCTGTGCGCCTGGCTACAAGCTGGGGGACGACCTCCTGCAGTGTCACCCCGCAGTGAAGtTCCCTTGTGGGAGGCCCTGGAAGCGGATGGAGAAG(+aag)CGCAGTCACCTGAAACGAGACACAGAAGACCAAGAAGACCAAGTAGATCCGCGGCTCATTGATG NG 10 8 20 8 30 0 100 5 10
    250713211754_vsuu CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250713170842_4i68 GGTGAGCTTCCTCAATTGCTCGCTGGACAACGGCGGCTGCACGCATTACTGCCTAGAGGAGGTGGGCTGGCGGCGCTGTAGCTGTGCGCCTGGCTACAAGCTGGGGGACGACCTCCTGCAGTGTCACCCCGCAGTGAAGTTCCCTTGTGGGAGGCCCTGGAAGCGGATGGAGAAG(-AAG)CGCAGTCACCTGAAACGAGACACAGAAGACCAAGAAGACCAAGTAGATCCGCGGCTCATTGATGGGAAGATGACCAGGCGGGGAGACAGCCCCTGGCAGGTGGTCCTGCTGGACTCAAAGAAGAAGCTGGCCTGCGGGGCAGTGCTCATCCACCCCTCCTGGGTGCTGACAGCGGCCCACTGCATGGATGAGTCCAAGAAGCTCCTTGTCAGGCTT NG 15 8 20 8 30 0 100 5 10