Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250114200112_depk CTGGAAATTGAGTTCTTTTTGAGTATATTAGGAATGCACTGTTTTAGTATTCAAGTCTATTTTCTTGGCCTAAAATAGTAATTGTACATCACATCACTTCCTGATTAATTACACATCATATTAATTGCTATATTAATGTTCCAAATAATGGAATATTTTTTTCAAAAACCATTGGCTCACACTTTGCCTTTGGTCATTAATGTAAAGTCATCCACCATCACCAATTGCTTTGTGTTTATAGCAAAATATACAGGCAAATCAGATAGTAAGGTTACAAAGCAAAAGAATAATCCACCCTTCTGCTTCCTTCCAAGAAAAAGCTCAGAAAACAAAGCAAATAGAAACAGTTGCAACACTTAGCACATGTGACATGGTTAATATCTAACCTTTATC(+G)CACTGGAGATTTGTCTGTTTGAGCTTCTTTTCAAGTTTATCTTGCTCTTCTGGCCTTATGGGAGCACTTACAAGTACTGCTCCTCCTGTTTCATTTAATTGTTTTAGAATTCCCTGGCGCAGGGGCAACTCTTCTGCCAGTAACTGAAACGGACAAATACAACCACATTTAAAATGAATTACAGTGCAACTCCAATTACCTTTGAGTCACCTTTATTGATTAGTCTATCAAAGTGAAGCGACTCAACTGAAGGTCCACTGGCACCTAAAATACCAAATACTTCCACAGAGCACAGAGCTGATGGGCTGAAACAACTGCATCCAAGAAGATAAACCACTACAGATGACTATTATTTGGTTCCCTTGAGAGTGTTTTCAAGTGAAAAATGTTACATGACATATAGAACT NGG 10 8 17 8 24 0 100 5 10
    250114144810_prhu CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250113235810_tpxn CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250113235804_fxj6 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250113235746_tdt5 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250107191921_laoq AGAACGCTCTCAAGTCGTCCCCGCACGCTTCTCTCGGAGGTTTCCCGGTGGAGAAGTACATGGACGTGTCGGGCCTGGCGAGCAGCAGCGTACCGACCAACGAGGTGCCCGCAAGAGCCAAGGAAGTGTCCTTCTACCAGGGCTACACAAGTCCCTATCAGCACGTGCCTGGCTACATCGACATGGTGTCCACTTTTGGATCCGGGGAACCTCGGCACGAGGCATACATCTCCATGGAGGGCTACCAGTCCTGGACGCTAGCCAACGGGTGGAACAGCCAGGT(+ctt)GTACTGTGCCAAGGATCAGCCACAGGGGTCCCATTTTTGGAAATCATCCTTTCCAGGTAGGAGCAACGAAAAAAGGGGAGGGAGAAAAAAGAAAAGCCCCTATTTGGGAGTGGTTACTTATATTCCAGGCCTGACCAGGTATAATCCACTAGAAAAATTTGTTTAGTCTCTGTGATCTCTGACTTTCCCAATTAAAA NGG 10 8 17 8 24 0 100 5 10
    250107191900_0gix AGAACGCTCTCAAGTCGTCCCCGCACGCTTCTCTCGGAGGTTTCCCGGTGGAGAAGTACATGGACGTGTCGGGCCTGGCGAGCAGCAGCGTACCGACCAACGAGGTGCCCGCAAGAGCCAAGGAAGTGTCCTTCTACCAGGGCTACACAAGTCCCTATCAGCACGTGCCTGGCTACATCGACATGGTGTCCACTTTTGGATCCGGGGAACCTCGGCACGAGGCATACATCTCCAT(+ctt)GGAGGGCTACCAGTCCTGGACGCTAGCCAACGGGTGGAACAGCCAGGTGTACTGTGCCAAGGATCAGCCACAGGGGTCCCATTTTTGGAAATCATCCTTTCCAGGTAGGAGCAACGAAAAAAGGGGAGGGAGAAAAAAGAAAAGCCCCTATTTGGGAGTGGTTACTTATATTCCAGGCCTGACCAGGTATAATCCACTAGAAAAATTTGTTTAGTCTCTGTGATCTCTGACTTTCCCAATTAAAA NGG 10 8 17 8 24 10 50 5 10
    250107191825_jtum AGAACGCTCTCAAGTCGTCCCCGCACGCTTCTCTCGGAGGTTTCCCGGTGGAGAAGTACATGGACGTGTCGGGCCTGGCGAGCAGCAGCGTACCGACCAACGAGGTGCCCGCAAGAGCCAAGGAAGTGTCCTTCTACCAGGGCTACACAAGTCCCTATCAGCACGTGCCTGGCTACATCGACATGGTGTCCACTTTTGGATCCGGGGAACCTCGGCACGAGGCATACATCTCCAT(+ctt)GGAGGGCTACCAGTCCTGGACGCTAGCCAACGGGTGGAACAGCCAGGTGTACTGTGCCAAGGATCAGCCACAGGGGTCCCATTTTTGGAAATCATCCTTTCCAGGTAGGAGCAACGAAAAAAGGGGAGGGAGAAAAAAGAAAAGCCCCTATTTGGGAGTGGTTACTTATATTCCAGGCCTGACCAGGTATAATCCACTAGAAAAATTTGTTTAGTCTCTGTGATCTCTGACTTTCCCAATTAAAA NGG 10 8 17 8 24 20 50 5 10
    250107191758_l8fu AGAACGCTCTCAAGTCGTCCCCGCACGCTTCTCTCGGAGGTTTCCCGGTGGAGAAGTACATGGACGTGTCGGGCCTGGCGAGCAGCAGCGTACCGACCAACGAGGTGCCCGCAAGAGCCAAGGAAGTGTCCTTCTACCAGGGCTACACAAGTCCCTATCAGCACGTGCCTGGCTACATCGACATGGTGTCCACTTTTGGATCCGGGGAACCTCGGCACGAGGCATACATCTCCAT(+ctt)GGAGGGCTACCAGTCCTGGACGCTAGCCAACGGGTGGAACAGCCAGGTGTACTGTGCCAAGGATCAGCCACAGGGGTCCCATTTTTGGAAATCATCCTTTCCAGGTAGGAGCAACGAAAAAAGGGGAGGGAGAAAAAAGAAAAGCCCCTATTTGGGAGTGGTTACTTATATTCCAGGCCTGACCAGGTATAATCCACTAGAAAAATTTGTTTAGTCTCTGTGATCTCTGACTTTCCCAATTAAAA NGG 10 8 17 8 24 30 100 5 10
    250107191659_0j4k AGAACGCTCTCAAGTCGTCCCCGCACGCTTCTCTCGGAGGTTTCCCGGTGGAGAAGTACATGGACGTGTCGGGCCTGGCGAGCAGCAGCGTACCGACCAACGAGGTGCCCGCAAGAGCCAAGGAAGTGTCCTTCTACCAGGGCTACACAAGTCCCTATCAGCACGTGCCTGGCTACATCGACATGGTGTCCACTTTTGGATCCGGGGAACCTCGGCACGAGGCATACATCTCCAT(+ctt)GGAGGGCTACCAGTCCTGGACGCTAGCCAACGGGTGGAACAGCCAGGTGTACTGTGCCAAGGATCAGCCACAGGGGTCCCATTTTTGGAAATCATCCTTTCCAGGTAGGAGCAACGAAAAAAGGGGAGGGAGAAAAAAGAAAAGCCCCTATTTGGGAGTGGTTACTTATATTCCAGGCCTGACCAGGTATAATCCACTAGAAAAATTTGTTTAGTCTCTGTGATCTCTGACTTTCCCAATTAAAA NGG 10 8 17 8 24 0 100 5 10