Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    260424104101_51gr GTGTAGTGTTCCTAGATTCAGCTGAAGACCTG(+CGCGATGCGATGCA)GTACCTAGGGTTCCTAACGGGATGGTGTTAG NGG 10 8 17 8 24 0 100 5 10
    260424104047_5ocx CAAAACCATCTGTAGCTCCTCCACTTTCAGGG(+CGCGATGCGATGCA)GATCCGGCACCCTCTGCAGGCACTAGGAGCA NGG 10 8 17 8 24 0 100 5 10
    260424104033_1jom ACTTCTGCTCCTAGTGCCTGCAGAGGGTGCCG(+CGCGATGCGATGCA)GATCCCCTGAAAGTGGAGGAGCTACAGATGG NGG 10 8 17 8 24 0 100 5 10
    260424104021_wh8i TGCATTTTCCTGACTGATGATTGATGTGGGAG(+CGCGATGCGATGCA)GATCCATCCCAATGTGGGCAACTATAAGAAA NGG 10 8 17 8 24 0 100 5 10
    260424104007_mts2 CCTGCTTTCTTATAGTTGCCCACATTGGGATG(+CGCGATGCGATGCA)GATCCTCCCACATCAATCATCAGTCAGGAAA NGG 10 8 17 8 24 0 100 5 10
    260424103951_bmfs CTCCCCATGGATTCATGTGCACCCATCTGGAG(+CGCGATGCGATGCA)GTACCAGATTCCTCCATGGCAGATCTGTTGG NGG 10 8 17 8 24 0 100 5 10
    260424103937_w364 TGTTCCCAACAGATCTGCCATGGAGGAATCTG(+CGCGATGCGATGCA)GTACCTCCAGATGGGTGCACATGAATCCATG NGG 10 8 17 8 24 0 100 5 10
    260424103854_h093 GACGCTTTTGTTAGAAACCTGAAGCCAGTTTG(+CGCGATGCGATGCA)GATCCTGGTGATCACTGTGTTATCAGTAGCA NGG 10 8 17 8 24 0 100 5 10
    260424103840_9dck AAGTGTTGTTGGGCAGTCCCGTGTCATCCATG(+CGCGATGCGATGCA)GTACCTGCTTCAATTTCACACATAGCTGGTA NGG 10 8 17 8 24 0 100 5 10
    260424103825_8nzi CAGTTTACCAGCTATGTGTGAAATTGAAGCAG(+CGCGATGCGATGCA)GTACCATGGATGACACGGGACTGCCCAACAA NGG 10 8 17 8 24 0 100 5 10