Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    260424100204_2rdj GCTACCCAGAGGCCTTTCGTCTCCCTGTTCTG(+TCAGCT)GTACCCAGCTTCCCAATCAGCAGCCAGAGCT NGG 10 8 17 8 24 0 100 5 10
    260424100132_t5kx TTGTTCTAGAAGGCGCTCCTTTCAGGGTTGTG(+TCAGCT)GTACCTGTAGGTTAGCAGACCCTCCTCCTTT NGG 10 8 17 8 24 0 100 5 10
    260424100104_lyca GTGGGAAAGGAGGAGGGTCTGCTAACCTACAG(+TCAGCT)GTACCACAACCCTGAAAGGAGCGCCTTCTAG NGG 6 8 17 8 24 0 100 5 10
    260424100042_r104 GTGGGAAAGGAGGAGGGTCTGCTAACCTACAG(+TCAGCT)GTACCACAACCCTGAAAGGAGCGCCTTCTAG NGG 10 8 17 8 24 0 100 5 10
    260424100004_qven GTGGGAAAGGAGGAGGGTCTGCTAACCTACAG(+TCAGCT)GTACCACAACCCTGAAAGGAGCGCCTTCTAG NGG 10 8 17 8 24 0 100 5 10
    260424095944_bh8p GTGTAGTGTTCCTAGATTCAGCTGAAGACCTG(+TCAGCT)GTACCTAGGGTTCCTAACGGGATGGTGTTAG NGG 10 8 17 8 24 0 100 5 10
    260424095928_9ibh CAAAACCATCTGTAGCTCCTCCACTTTCAGGG(+TCAGCT)GATCCGGCACCCTCTGCAGGCACTAGGAGCA NGG 10 8 17 8 24 0 100 5 10
    260424095908_wlux ACTTCTGCTCCTAGTGCCTGCAGAGGGTGCCG(+TCAGCT)GATCCCCTGAAAGTGGAGGAGCTACAGATGG NGG 10 8 17 8 24 0 100 5 10
    260424095847_b7s7 TGCATTTTCCTGACTGATGATTGATGTGGGAG(+TCAGCT)GATCCATCCCAATGTGGGCAACTATAAGAAA NGG 10 8 17 8 24 0 100 5 10
    260424095830_l6db CCTGCTTTCTTATAGTTGCCCACATTGGGATG(+TCAGCT)GATCCTCCCACATCAATCATCAGTCAGGAAA NGG 10 8 17 8 24 0 100 5 10