Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    260424103016_dqwp CTCTCCCACCCGGCTCTAGGCCAAAAGGCTCG(+CGTATC)GTACCTCCTTACTGGGAGCCACGTAAATGGA NGG 10 8 17 8 24 0 100 5 10
    260424103003_v78t AAGGTTCCATTTACGTGGCTCCCAGTAAGGAG(+CGTATC)GTACCGAGCCTTTTGGCCTAGAGCCGGGTGG NGG 10 8 17 8 24 0 100 5 10
    260424102949_5mnk TACCCCTCCTGTGTGTGAAGGCTCTGCTGAAG(+CGTATC)GTACCTTTGGGTCTGGTCTGACCTTACTGCT NGG 10 8 17 8 24 0 100 5 10
    260424102934_swda GGTCACAGGGACAGCTCCGCTGCTGCCTCTGG(+CGTATC)GTACCTTGAAAAGATGCCCACCACGCACCTT NGG 10 8 17 8 24 0 100 5 10
    260424102911_andq CTGCTGCCCCTGGGTGCAGGCAGTAGCCAGGG(+CGTATC)GATCCAGGGCCTCAGATCCAGGGTCCTGCAT NGG 10 8 17 8 24 0 100 5 10
    260424102857_50vz AGTTGTGAAAGCAAGGAGTACAGAATGCTAGG(+CGTATC)GATCCTCTCAAAAGTTCCAAACTAATTCTGT NGG 10 8 17 8 24 0 100 5 10
    260424102840_oaeq AGGCCTAGGACCAAGGTATCCTCTGTCTGCAG(+CGTATC)GTACCTGACTACCCACAACAGGGAGGCTCGC NGG 10 8 17 8 24 0 100 5 10
    260424102826_i0np AGATGGCGAGCCTCCCTGTTGTGGGTAGTCAG(+CGTATC)GTACCTGCAGACAGAGGATACCTTGGTCCTA NGG 10 8 17 8 24 0 100 5 10
    260424102813_jxx7 TGCAGAGATGGTTTATGAGGCGGGTCCGAAGG(+CGTATC)GTACCCTTCACCTTGCCTGGAGATATACAAA NGG 10 8 17 8 24 0 100 5 10
    260424102800_1l6g TCTGTGCTCAGGTCACACAGGGGGCTTCAGAG(+CGTATC)GATCCTTATGAAAGATGAGTTAAAAAATGTC NGG 10 8 17 8 24 0 100 5 10