Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    241219203339_jeln AGCACTACAGGCAGCACCAGCGAAGGGGCCGTGACAGCCGTTGCCATCTGCTGCCGGAG(/A)CCGGCACCTGGCGCAGGCCTCCCAGGAGCTCCAGTGACAGC NGG 10 8 17 8 24 0 100 5 10
    241219173000_9jmn TGTGGCCTGGGGACAGCGTCCGGGCCTCGAACTCCAGGTGTCTTTCAAAGTAGTGG(-GAG)GCTTGGGAGATTTCCCAGCTTATGTTGCATCTGTGGGTCTC NGG 10 8 17 8 24 0 100 5 10
    241219172127_egsu TGTGGCCTGGGGACAGCGTCCGGGCCTCGAACTCCAGGTGTCTTTCAAAGTAGTGG(-GAG)GCTTGGGAGATTTCCCAGCTTATGTTGCATCTGTGGGTCTC NGG 10 8 17 8 24 0 100 5 10
    241219171349_l4ux TGTGGCCTGGGGACAGCGTCCGGGCCTCGAACTCCAGGTGTCTTTCAAAGTAG(+C)TGGGAGGCTTGGGAGATTTCCCAGCTTATGTTGCATCTGTGGGTCTC NGG 10 8 17 8 24 0 100 5 10
    241219170918_bhlb TGTGGCCTGGGGACAGCGTCCGGGCCTCGAACTCCAGGTGTCTTTCAAAGTAG(+C)TGGGAGGCTTGGGAGATTTCCCAGCTTATGTTGCATCTGTGGGTCTC NGG 10 8 17 8 24 0 100 5 10
    241219170825_4x97 TGTGGCCTGGGGACAGCGTCCGGGCCTCGAACTCCAGGTGTCTTTCAAAGTAG(/C)TGGGAGGCTTGGGAGATTTCCCAGCTTATGTTGCATCTGTGGGTCTC NGG 10 8 17 8 24 0 100 5 10
    241219170811_6on1 TGTGGCCTGGGGACAGCGTCCGGGCCTCGAACTCCAGGTGTCTTTCAAAGTAG(/C)TGGGAGGCTTGGGAGATTTCCCAGCTTATGTTGCATCTGTGGGTCTC NGG 10 8 17 8 24 0 100 5 10
    241219161117_pcxr AACCTGGCCATCTCTGACCTGTTTTTCCTTCTTACTGTC(-C)CCTTCTGGGCTCACTATGCTGCCGCCCAGTGGGACTTTGGAAATACAATGTGTCAACTCT NGG 10 8 17 8 24 0 100 5 10
    241219160126_qynz AACCTGGCCATCTCTGACCTGTTTTTCCTTCTTACTGT(/G)CCCCTTCTGGGCTCACTATGCTGCCGCCCAGTGGGACTTTGGAAATACAATGTGTCAACTCT NGG 10 8 17 8 24 0 100 5 10
    241219155851_ct6y AACCTGGCCATCTCTGACCTGTTTTTCCTTCTTACTGT(/G)CCCCTTCTGGGCTCACTATGCTGCCGCCCAGTGGGACTTTGGAAATACAATGTGTCAACTCT NGG 10 8 17 8 24 0 100 5 10