Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    241212093829_83w9 ACGTGGACAAAGCTGTCAGCGGCCTCCGCAGTCTCACCTCCCTGCTTCGGGCGCTGGGAGCCCAGGTGTGTCTGGCCCCTTCCTTGCCCCTGGGGTTGCTGTTTCTGTAAAGTGAGAGAAGGGCCC(-TGCCAAGGAAACCCCCC)AGCCATTCTACCCTGAAACCCATCTCCTGCCGGAGCCCCTGGCACTGCACTAACCTTTAGTCCTCTCTTTGCAGAAAGAAGCCATCTCCCTTCCAGATGCAACCCCCTCCGCAGCCCCACTCCGAGCATTCACTGTTGATGCTTTG NGG 10 8 17 8 24 0 100 5 10
    241211063828_p0nd ATGACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGACGTCCCCAGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCACACCGTCGATCCGGACCGCCACATCGAG(/C)GAGTCACCGAGCTGCAAGAACTCTTCCTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGCGCCGCCGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGCCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTCGGAGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGGTGCCCGAAGGACCGCGCACCTGGTGCATGACCCGCAAGCCCGGTGCCTGA NGG 10 8 17 8 24 0 100 5 10
    241210183239_sbcw cggtgcctaccatggctgatgacctgggggagccagccccactctggccacatgaccctga(/t)cgccaaggagaagcccctccacatgcctgggggagatggtcaccgggggaaaagcctgaagctgctggagaagatccctgaagatgctgaggccacggttgtgcttgtgggttgtgtgcctttcttggagcagcctgcagcagccttcgtgcgtctgaatgaggctgtactcctggagt NGG 10 8 17 8 24 0 100 5 10
    241210174828_0b21 tttttgggtgccttgcaaagttcccggggctcttctctctctaggaagcagagctgagactggtgaaattcttgcctgagattctggccctgcaaaagaatctggtgaagcgattccagaatgtttcggaagttgaatacagctgcatcag(/a)aagcttcatcatcagtcacagttcaggtgggcctctgtttcagcagtggttcggaagcgtcgctgcccctcgggctccggttccggtccgggtgctctgccctgagctggcagctcgaagccgtgaatgccgcgg NGG 35 8 15 0 50 50 900 5 10
    241210143657_qm7e CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    241208100110_yyhu AACCTGCCCAGGGCCTCACCACCAACTTCATCCACGTTCACCTTGCCCCACAGGGCAGTAAC(-G)GCAGACTTCTCCTCAGGAGTCAGATGCACCATGGTGT NGG 10 8 17 8 24 0 100 5 10
    241208095909_t9tc AACCTGCCCAGGGCCTCACCACCAACTTCATCCACGTTCACCTTGCCCCACAGGGCAGTAAC(-G)GCAGACTTCTCCTCAGGAGTCAGATGCACCATGGTGT NGG 10 8 17 8 24 0 100 5 10
    241208095630_r564 AACCTGCCCAGGGCCTCACCACCAACTTCATCCACGTTCACCTTGCCCCACAGGGCAGTAACG(-G)CAGACTTCTCCTCAGGAGTCAGATGCACCATGGTGT NGG 10 8 17 8 24 0 100 5 10
    241208094632_yjyy AACCTGCCCAGGGCCTCACCACCAACTTCATCCACGTTCACCTTGCCCCACAGGGCAGTAACGGCA(+TGC)GACTTCTCCTCAGGAGTCAGATGCACCATGGTGT NGG 10 8 17 8 24 0 100 5 10
    241208094238_ucne AACCTGCCCAGGGCCTCACCACCAACTTCATCCACGTTCACCTTGCCCCACAGGGCAGTAACGGCA(+TGC)GACTTCTCCTCAGGAGTCAGATGCACCATGGTGT NGG 10 8 17 8 24 0 100 5 10