Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    241205172249_4gcw ATGAAGCACATGGCTGGTGCTGCAGCAGCTGGGGCAGTGGTGGGG(-GGC)CTTGGCGGCTACATGCTGGGAAGTGCCATGAGCAGGCCCATCATACATTTCG NGG 10 8 17 8 24 0 100 5 10
    241205172247_xufy ATGAAGCACATGGCTGGTGCTGCAGCAGCTGGGGCAGTGGTGGGG(-GGC)CTTGGCGGCTACATGCTGGGAAGTGCCATGAGCAGGCCCATCATACATTTCG NGG 10 8 17 8 24 0 100 5 10
    241205172219_uknb ATGAAGCACATGGCTGGTGCTGCAGCAGCTGGGGCAGTGGTGGGG(-GGC)CTTGGCGGCTACATGCTGGGAAGTGCCATGAGCAGGCCCATCATACATTTCG NGG 10 8 17 8 24 0 100 5 10
    241205172128_9564 ATGAAGCACATGGCTGGTGCTGCAGCAGCTGGGGCAGTGGTGGGGG(-GCC)TTGGCGGCTACATGCTGGGAAGTGCCATGAGCAGGCCCATCATACATTTCG NGG 10 8 17 8 24 0 100 5 10
    241205172006_o0vw ATGAAGCACATGGCTGGTGCTGCAGCAGCTGGGGCAGTGGTGGG(-GGG)CCTTGGCGGCTACATGCTGGGAAGTGCCATGAGCAGGCCCATCATACATTTCG NGG 10 8 17 8 24 0 100 5 10
    241205171526_iiwi ATGAAGCACATGGCTGGTGCTGCAGCAGCTGGGGCAGTGGTGGGGGGCCTTGGCGGC(/T)TACATGCTGGGAAGTGCCATGAGCAGGCCCATCATACATTTCG NGG 10 8 17 8 24 0 100 5 10
    241205165735_etw4 ATGAAGCACATGGCTGGTGCTGCAGCAGCTGGGGCAGTGGTGGGGGGCCTTGGCGGC(/T)TACATGCTGGGAAGTGCCATGAGCAGGCCCATCATACATTTCG NGG 10 8 17 8 24 0 100 5 10
    241205165626_rlm4 ATGAAGCACATGGCTGGTGCTGCAGCAGCTGGGGCAGTGGTGGGGGGCCTTGGCGGC(/T)TACATGCTGGGAAGTGCCATGAGCAGGCCCATCATACATTTCG NGG 10 8 17 8 24 0 100 5 10
    241205164821_je57 ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTGCAGGCCAGATGAGGGC(+ATA)TCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241205164654_vadf ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTGCAGGCCAGATGAGGGC(+ATA)TCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10