Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    260423212354_40hq GTGGGAAAGGAGGAGGGTCTGCTAACCTACAG(+CGTACGCATCGTCG)GTACCACAACCCTGAAAGGAGCGCCTTCTAG NGG 10 8 17 8 24 0 100 5 10
    260423212334_sjx6 GTGTAGTGTTCCTAGATTCAGCTGAAGACCTG(+CGTACGCATCGTCG)GTACCTAGGGTTCCTAACGGGATGGTGTTAG NGG 10 8 17 8 24 0 100 5 10
    260423212315_cffk CAAAACCATCTGTAGCTCCTCCACTTTCAGGG(+CGTACGCATCGTCG)GATCCGGCACCCTCTGCAGGCACTAGGAGCA NGG 10 8 17 8 24 0 100 5 10
    260423212258_w7s8 ACTTCTGCTCCTAGTGCCTGCAGAGGGTGCCG(+CGTACGCATCGTCG)GATCCCCTGAAAGTGGAGGAGCTACAGATGG NGG 10 8 17 8 24 0 100 5 10
    260423212236_g838 TGCATTTTCCTGACTGATGATTGATGTGGGAG(+CGTACGCATCGTCG)GATCCATCCCAATGTGGGCAACTATAAGAAA NGG 10 8 17 8 24 0 100 5 10
    260423212150_qjd9 CCTGCTTTCTTATAGTTGCCCACATTGGGATG(+CGTACGCATCGTCG)GATCCTCCCACATCAATCATCAGTCAGGAAA NGG 10 8 17 8 24 0 100 5 10
    260423212130_vuv6 CTCCCCATGGATTCATGTGCACCCATCTGGAG(+CGTACGCATCGTCG)GTACCAGATTCCTCCATGGCAGATCTGTTGG NGG 10 8 17 8 24 0 100 5 10
    260423212105_pdlf TGTTCCCAACAGATCTGCCATGGAGGAATCTG(+CGTACGCATCGTCG)GTACCTCCAGATGGGTGCACATGAATCCATG NGG 10 8 17 8 24 0 100 5 10
    260423212039_ou9r GACGCTTTTGTTAGAAACCTGAAGCCAGTTTG(+CGTACGCATCGTCG)GATCCTGGTGATCACTGTGTTATCAGTAGCA NGG 10 8 17 8 24 0 100 5 10
    260423211918_woq7 AAGTGTTGTTGGGCAGTCCCGTGTCATCCATG(+CGTACGCATCGTCG)GTACCTGCTTCAATTTCACACATAGCTGGTA NGG 10 8 17 8 24 0 100 5 10