Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    241204213824_x469 ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTAGCAGGCCAGATG(+T)AGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204213758_21ci ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTAGCAGGCCAGAT(+C)GAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204213740_a68a ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTAGCAGGCCAGA(+C)TGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204213740_3wb7 ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTAGCAGGCCAGA(+C)TGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204213724_nmga ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTAGCAGGCCAG(+T)ATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204213643_c4y2 ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTAGCAGGCCA(+C)GATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204213620_y3u8 ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTAGCAGG(+A)CCAGATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204213603_7x5w ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTAGCAGGCC(+T)AGATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204213508_kvfq ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTAGCAGGC(+T)CAGATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204213445_gyye ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTAGCA(+A)GGCCAGATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10