Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    241204210338_e9pr ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTGCAG(-G)CCAGATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204210323_rrlz ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTGCA(-G)GCCAGATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204210307_pe95 ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTGC(-A)GGCCAGATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204210252_xlkf ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTG(-C)AGGCCAGATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204210235_d9xu ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCT(-G)CAGGCCAGATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204210215_o3iw ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTC(-T)GCAGGCCAGATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204203813_xkp8 ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTGCA(-GGC)CAGATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204203008_3fmv ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTGCA(-GGC)CAGATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204202659_kuh6 ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTGCA(-GGC)CAGATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204202642_o6ae ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTGCAG(-GCC)AGATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10