Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".
  • Substitution is indicated by backslash "/", and the example is used to replace A by T.
  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position
  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.
  • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    241204212735_7wh0 ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTGCAGGCC(-A)GATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204212719_ygdb ACCCCAGTCACTCCAGCCTGTTGGCTGCC(+A)GCTCACTTTGATGTCTGCAGGCCAGATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204212642_2npl ACCCCAGTCACTCCAGCCTGTTGGCTGCC(+A)GCTCACTTTGATGTCTGCAGGCCAGATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204212628_yu5g ACCCCAGTCACTCCAGCCTGTTGGCT(+C)GCCGCTCACTTTGATGTCTGCAGGCCAGATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204212619_8zj2 ACCCCAGTCACTCCAGCCTGTTGGCT(+G)GCCGCTCACTTTGATGTCTGCAGGCCAGATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204212603_blby ACCCCAGTCACTCCAGCCTGTTG(+A)GCTGCCGCTCACTTTGATGTCTGCAGGCCAGATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204212540_iz06 ACCCCAGTCACTCCAGCCTGTTGGC(+A)TGCCGCTCACTTTGATGTCTGCAGGCCAGATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204212452_ltym ACCCCAGTCACTCCAGCCTGTTGGCTGC(+A)CGCTCACTTTGATGTCTGCAGGCCAGATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204210912_wnn1 ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTGCAGGCC(-A)GATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204210820_lnvv ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTGCAGGCCAGATGAGG(-G)CTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10