Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250418195924_84nt CCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGCAGCTACGGTTTCCGTCTGGGCTTCTTGCATTCTGGGACAGCCAAGTCTGTGACTTGCACG(/T)GTCAGTTGCCCTGAGGGGCTGGCTTCCATGAGACTTCAATGCCTGGCCGTATCCCCCTGCATTTCTTTTGTTTGGAACTTTGGGATTCCTCTTCACCCTT NGG 10 8 17 8 24 0 100 5 10
    250416193829_pb3f GTCTCCGGCCGCCTCGCTCTCCCCCCTTCGCGCTCGCCCTCGCGCTCGACCCATCTGGGCTGGGGCACGCGGCGACACTCACTCGTCGGCCCAGGGACCCCCCGGTCTCGAAACGTCGACACATTTAATGCTTAATCTAACAATAATTACTTTTATATATTTGATCGAACTTTAAAAGATTTGGCTCCTCAGAAAGATAGACGATGTGCATTTATTTTAGAAATGATAGGATATATTTAAGGCGACATATGGAATAGA(+ATT)CAGGTTGGGATGGAGTAGATATATATAGATTCATACGACTAAAGTTGTGTTCAGCAGATCATATATATAGCTATGCAAAACACTATTTTACACTGTAGATAGCAATATTTATGTTGTTTAGAGAGTGAATTTTGAAATAAGAGGTGAGATAGAGAATGTGATATGGCAGCTAAGAGCTAGTTTTCAACAAGTTTGTTTCTATAATAGTATAGCTAGATAGACAGACACGTAGCTAAAATGGTCAACTGACCTTCGTACAAAAA NG 10 8 17 8 24 0 100 5 10
    250416193623_kkje CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NG 10 8 17 8 24 0 100 5 10
    250416193622_lhe3 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NG 10 8 17 8 24 0 100 5 10
    250416193622_it29 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NG 10 8 17 8 24 0 100 5 10
    250416193622_ck3p CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NG 10 8 17 8 24 0 100 5 10
    250416193619_t3va CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NG 10 8 17 8 24 0 100 5 10
    250416193531_weyz CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250416112900_3473 ATTAATGGACTTCAGTGGCTGGGTTGGTGAGGGAAAGAATGAAAAAGAGTCCTACAGGACCACTGTCATTCCAGGGTAACCCTGGATATACTCAAAGCTGT(/G)GCCTCCTTGAGGTAACATCAAAAGCTTAACACTGTGGGATGAGGAGATGAAGATAGACTTTATTAAAATAATCCAGACAATCATTGTTAATCACATAAACA NGG 10 8 17 8 24 0 100 5 10
    250415114910_uhu1 TGACAAGAAGGCCAAGATTGCAACACCGTCAGGCCAGAAGACAGGTGACAAGAAGGGAGCTGTCCATGTGGCGACTCCTCACCCGGCCAAGAAGGCAGGCAAGACCCCGGCTACCAGCGACAAGTCGCCCAAGTCTG(+AAGTCTG)GAGGGTCGGTCGCGTGCAAGTCGTGCAGCAAGACATTCAACAGCGAGGGCGCTCTAGCTTCCCACTCCAAGGCAAAGCACGAGGCCAAGTGA NGG 10 8 17 8 24 0 100 5 10