Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    241204214153_1n4k ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTAGCAGGCCAGATGAGGGCTCCAGA(+T)TGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204214132_vs75 ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTAGCAGGCCAGATGAGGGCTCCAG(+C)ATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204214111_16j5 ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTAGCAGGCCAGATGAGGGCTCCA(+C)GATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204214051_jw2r ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTAGCAGGCCAGATGAGGGCTC(+A)CAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204214030_a64l ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTAGCAGGCCAGATGAGGGCTCC(+T)AGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204214009_kdxm ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTAGCAGGCCAGATGAGGGCTC(+G)CAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204213954_rxw7 ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTAGCAGGCCAGATGAGGGC(+G)TCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204213922_746z ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTAGCAGGCCAGATGAGG(+A)GCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204213905_zfit ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTAGCAGGCCAGATGAG(+C)GGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204213843_d5c3 ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTAGCAGGCCAGATGA(+T)GGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10