Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    241204213430_4os1 ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTAGC(+T)AGGCCAGATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204213413_hsxa ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTAG(+T)CAGGCCAGATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204213352_bbaa ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTA(+G)GCAGGCCAGATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204213327_5e5j ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCT(+A)GCAGGCCAGATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204213308_4tr9 ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTC(+A)TGCAGGCCAGATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204212945_b17g ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTC(+T)ACTTTGATGTCTGCAGGCCAGATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204212926_hoyn ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCT(+T)CACTTTGATGTCTGCAGGCCAGATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204212851_4m6f ACCCCAGTCACTCCAGCCTGTTGGCTGCCGC(+A)TCACTTTGATGTCTGCAGGCCAGATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204212824_7s7r ACCCCAGTCACTCCAGCCTGTTGGCTGCCG(+T)CTCACTTTGATGTCTGCAGGCCAGATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241204212807_q9ag ACCCCAGTCACTCCAGCCTGTTGGCTGCC(+A)GCTCACTTTGATGTCTGCAGGCCAGATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10