Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    241205174137_e825 ATGAAGCACATGGCTGGTGCTGCAGCAGCTGGGGCAGTGGTGGGGGGCCTTGGCGGCT(-A)CATGCTGGGAAGTGCCATGAGCAGGCCCATCATACATTTCG NGG 10 8 17 8 24 0 100 5 10
    241205174048_gkga ATGAAGCACATGGCTGGTGCTGCAGCAGCTGGGGCAGTGGTGGGGGGCCTTGGCGGC(-T)ACATGCTGGGAAGTGCCATGAGCAGGCCCATCATACATTTCG NGG 10 8 17 8 24 0 100 5 10
    241205173751_mgyj ATGAAGCACATGGCTGGTGCTGCAGCAGCTGGGGCAGTGGTGGGGGGCCTTGGCGGC(-T)ACATGCTGGGAAGTGCCATGAGCAGGCCCATCATACATTTCG NGG 10 8 17 8 24 0 100 5 10
    241205173553_x4gd ATGAAGCACATGGCTGGTGCTGCAGCAGCTGGGGCAGTGGTGGGGGGCCTTGGCGGC(-T)ACATGCTGGGAAGTGCCATGAGCAGGCCCATCATACATTTCG NGG 10 8 17 8 24 0 100 5 10
    241205172522_eumq ATGAAGCACATGGCTGGTGCTGCAGCAGCTGGGGCAGTGGTGGGG(-GGC)CTTGGCGGCTACATGCTGGGAAGTGCCATGAGCAGGCCCATCATACATTTCG NGG 10 8 17 8 24 0 100 5 10
    241205172520_jovk ATGAAGCACATGGCTGGTGCTGCAGCAGCTGGGGCAGTGGTGGGG(-GGC)CTTGGCGGCTACATGCTGGGAAGTGCCATGAGCAGGCCCATCATACATTTCG NGG 10 8 17 8 24 0 100 5 10
    241205172258_qoko ATGAAGCACATGGCTGGTGCTGCAGCAGCTGGGGCAGTGGTGGGG(-GGC)CTTGGCGGCTACATGCTGGGAAGTGCCATGAGCAGGCCCATCATACATTTCG NGG 10 8 17 8 24 0 100 5 10
    241205172257_v1vp ATGAAGCACATGGCTGGTGCTGCAGCAGCTGGGGCAGTGGTGGGG(-GGC)CTTGGCGGCTACATGCTGGGAAGTGCCATGAGCAGGCCCATCATACATTTCG NGG 10 8 17 8 24 0 100 5 10
    241205172254_kwws ATGAAGCACATGGCTGGTGCTGCAGCAGCTGGGGCAGTGGTGGGG(-GGC)CTTGGCGGCTACATGCTGGGAAGTGCCATGAGCAGGCCCATCATACATTTCG NGG 10 8 17 8 24 0 100 5 10
    241205172253_qnbs ATGAAGCACATGGCTGGTGCTGCAGCAGCTGGGGCAGTGGTGGGG(-GGC)CTTGGCGGCTACATGCTGGGAAGTGCCATGAGCAGGCCCATCATACATTTCG NGG 10 8 17 8 24 0 100 5 10