Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    260422143333_lfuu TAACTTACAACTTACATGAAAGAAGAGTGGAG(+ACTCACCGTACGTA)GATCCTGTTGGATTGTTTGGCTGGACACTAT NGG 10 8 17 8 24 0 100 5 10
    260422143246_5dfj TAACTTACAACTTACATGAAAGAAGAGTGGAG(+ACTCACATGCTAGC)GATCCTGTTGGATTGTTTGGCTGGACACTAT NGG 10 8 17 8 24 0 100 5 10
    260422143023_kmlg TAACTTACAACTTACATGAAAGAAGAGTGGAG(+ACTCACACTCAC)GATCCTGTTGGATTGTTTGGCTGGACACTAT NGG 10 8 17 8 24 0 100 5 10
    260422143001_35od TAACTTACAACTTACATGAAAGAAGAGTGGAG(+ACTCACACTCACAC)GATCCTGTTGGATTGTTTGGCTGGACACTAT NGG 10 8 17 8 24 0 100 5 10
    260422141542_3b3k ATTAATTAGGGTGGCTTGATCAGCTACAGAAG(+ACTCAC)GATCCTTTGGGGTCACATTCTTTTATCCTGT NGG 10 8 17 8 24 0 100 5 10
    260422141501_cp5v CAGTTTACCAGCTATGTGTGAAATTGAAGCAG(+ACTCAC)GTACCATGGATGACACGGGACTGCCCAACAA NGG 10 8 17 8 24 0 100 5 10
    260422141355_yq39 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    260422132613_dl9o CAATGATGCCTATATGTACCCTTAACATGTTACATATGAGGTCAAGTTGAGAAGTAACTTACAACTTACATGAAAGAAGAGTGGAG(+ACTCACACTCACAC)GATCCTGTTGGATTGTTTGGCTGGACACTATTACATTGGAACTGTGTTCACAGAATCAAAGCTGTGTTCCACCTGGGAGGGTGAGTCTTCAGAGTTCATCAGCT NGG 10 8 17 8 24 0 100 5 10
    260422132302_mk6p CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    260421213752_mn6k CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+CGG)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10