Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    260423205930_vlm6 GTGGGAAAGGAGGAGGGTCTGCTAACCTACAG(+TATCGCGATACGCG)GTACCACAACCCTGAAAGGAGCGCCTTCTAG NGG 10 8 17 8 24 0 100 5 10
    260423205913_49ug GTGGGAAAGGAGGAGGGTCTGCTAACCTACAG(+CGCGATATACGTCG)GTACCACAACCCTGAAAGGAGCGCCTTCTAG NGG 10 8 17 8 24 0 100 5 10
    260423205846_lhou GTGGGAAAGGAGGAGGGTCTGCTAACCTACAG(+GCGTATACGCGATC)GTACCACAACCCTGAAAGGAGCGCCTTCTAG NGG 10 8 17 8 24 0 100 5 10
    260423205828_md6c GTGGGAAAGGAGGAGGGTCTGCTAACCTACAG(+CGATATCGCGTACG)GTACCACAACCCTGAAAGGAGCGCCTTCTAG NGG 10 8 17 8 24 0 100 5 10
    260423205803_okvw GTGGGAAAGGAGGAGGGTCTGCTAACCTACAG(+ATCGCGTATACGCG)GTACCACAACCCTGAAAGGAGCGCCTTCTAG NGG 10 8 17 8 24 0 100 5 10
    260423205742_4pej GTGGGAAAGGAGGAGGGTCTGCTAACCTACAG(+CGTACGCGATATGC)GTACCACAACCCTGAAAGGAGCGCCTTCTAG NGG 10 8 17 8 24 0 100 5 10
    260423205715_18if GTGGGAAAGGAGGAGGGTCTGCTAACCTACAG(+GCGATCGTATACGC)GTACCACAACCCTGAAAGGAGCGCCTTCTAG NGG 10 8 17 8 24 0 100 5 10
    260423205637_bk1v GTGGGAAAGGAGGAGGGTCTGCTAACCTACAG(+CGATCGCGTATACG)GTACCACAACCCTGAAAGGAGCGCCTTCTAG NGG 10 8 17 8 24 0 100 5 10
    260423205301_qm6q TAAGTGCTTTGCCTGCTGTGGGAGAGGGACTG(+CGATCGCGTATACG)GATCCCGTCCTCTAACGTCTGTCTCTGGCCG NGG 10 8 17 8 24 0 100 5 10
    260423205246_ou6q TAAGTGCTTTGCCTGCTGTGGGAGAGGGACTG(+CGATCGCGTACGCG)GATCCCGTCCTCTAACGTCTGTCTCTGGCCG NGG 10 8 17 8 24 0 100 5 10