Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    260423205152_7t87 TAAGTGCTTTGCCTGCTGTGGGAGAGGGACTG(+CGCGCGCGCGCGCG)GATCCCGTCCTCTAACGTCTGTCTCTGGCCG NGG 10 8 17 8 24 0 100 5 10
    260423205129_bxkn CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    260423135857_ns1y TTGGGCATAGTCAGGGACTCTCGTGGGACCCCCCGAGTAGAAACACAGATGTGTCTCCCTGGGTCCCTGCCAGGTC(-CCCTCTCAGCCTGGATGGCTTCCCT)CCCTCTCTTTACCTTATTTATAGCCCAAGATTTCCTGGTTCAAGAATGGCCTGGACCTGGGAGAAGACGCCCGCTT NGG 10 8 17 8 24 0 100 5 10
    260423135520_922b TTGGGCATAGTCAGGGACTCTCGTGGGACCCCCCGAGTAGAAACACAGATGTGTCTCCCTGGGTCCCTGCCAGGTC(-CCCTCTCAGCCTGGATGGCTTCCCT)CCCTCTCTTTACCTTATTTATAGCCCAAGATTTCCTGGTTCAAGAATGGCCTGGACCTGGGAGAAGACGCCCGCTTCCGCATGTTCAGC NGG 10 8 17 8 24 0 100 5 10
    260423135023_aphv GCATAGTCAGGGACTCTCGTGGGACCCCCCGAGTAGAAACACAGATGTGTCTCCCTGGGTCCCTGCCAGGTCCCCTCTC(-AGCCTGGATGGCTTCCCTCCCTCTC)TTTACCTTATTTATAGCCCAAGATTTCCTGGTTCAAGAATGGCCTGGACCTGGGAGAAGACGCCCGCTT NGG 10 8 17 8 24 0 100 5 10
    260422144019_gml7 TAACTTACAACTTACATGAAAGAAGAGTGGAG(+CGCGCGCGCGCGCG)GATCCTGTTGGATTGTTTGGCTGGACACTAT NGG 10 8 17 8 24 0 100 5 10
    260422144005_44wg TAACTTACAACTTACATGAAAGAAGAGTGGAG(+GCGCGCGCGCGCGC)GATCCTGTTGGATTGTTTGGCTGGACACTAT NGG 10 8 17 8 24 0 100 5 10
    260422143547_ueud TAACTTACAACTTACATGAAAGAAGAGTGGAG(+CGCGCGCGCGCGCG)GATCCTGTTGGATTGTTTGGCTGGACACTAT NGG 10 8 17 8 24 0 100 5 10
    260422143519_jn2d TAACTTACAACTTACATGAAAGAAGAGTGGAG(+AGGACAAGGACAAG)GATCCTGTTGGATTGTTTGGCTGGACACTAT NGG 10 8 17 8 24 0 100 5 10
    260422143450_x7xd TAACTTACAACTTACATGAAAGAAGAGTGGAG(+ACTCACACTCACAC)GATCCTGTTGGATTGTTTGGCTGGACACTAT NGG 10 8 17 8 24 0 100 5 10