Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    241205194324_dw0r ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTGCAGGCCAGATGAGGGC(+ATA)TCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241205194245_4gl5 ACCCCAGTCACTCCAGCCTGTTGGCTGC(+A)CGCTCACTTTGATGTCTGCAGGCCAGATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241205194156_wjg7 ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTGCAGGCC(-A)GATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241205180913_y5uf ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTGCA(-GGC)CAGATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241205180830_erpn ACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTGCAGGCC(/T)AGATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    241205180136_gon5 ATGAAGCACATGGCTGGTGCTGCAGCAGCTGGGGCAGTGGTGGGGGGCCTTGGCGGCTACATGC(+GAA)TGGGAAGTGCCATGAGCAGGCCCATCATACATTTCG NGG 10 8 17 8 24 0 100 5 10
    241205180134_n4y1 ATGAAGCACATGGCTGGTGCTGCAGCAGCTGGGGCAGTGGTGGGGGGCCTTGGCGGCTACATGC(+GAA)TGGGAAGTGCCATGAGCAGGCCCATCATACATTTCG NGG 10 8 17 8 24 0 100 5 10
    241205175230_hxhv ATGAAGCACATGGCTGGTGCTGCAGCAGCTGGGGCAGTGGTGGGGGGCCTTGGCGGCTACATGC(+GAA)TGGGAAGTGCCATGAGCAGGCCCATCATACATTTCG NGG 10 8 17 8 24 0 100 5 10
    241205175039_gu2y ATGAAGCACATGGCTGGTGCTGCAGCAGCTGGGGCAGTGGTGG(+TAA)GGGGCCTTGGCGGCTACATGCTGGGAAGTGCCATGAGCAGGCCCATCATACATTTCG NGG 10 8 17 8 24 0 100 5 10
    241205174640_irpj ATGAAGCACATGGCTGGTGCTGCAGCAGCTGGGGCAGTGGTGGGGGGCCTTGGCGGCTACAT(+TGC)GCTGGGAAGTGCCATGAGCAGGCCCATCATACATTTCG NGG 10 8 17 8 24 0 100 5 10