Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    260423212930_rx3a GTATGCGTTCATGGTGGAACACAACCACATCG(+CGTACGCATCGTCG)GTACCTACTTCAGAACAATTATCTGAGAAAA NGG 10 8 17 8 24 0 100 5 10
    260423212838_9nxm TCAGTCTCAGCACTCAGAGACAGAGCTAGGTG(+CGTACGCATCGTCG)GATCCCTGAGTTCGAGGCCAGCCTGGTCTAC NGG 10 8 17 8 24 0 100 5 10
    260423212822_ycnz TCTCTGTAGACCAGGCTGGCCTCGAACTCAGG(+CGTACGCATCGTCG)GATCCACCTAGCTCTGTCTCTGAGTGCTGAG NGG 10 8 17 8 24 0 100 5 10
    260423212737_cljx AGACTCGGCCAGAGACAGACGTTAGAGGACGG(+CGTACGCATCGTCG)GATCCAGTCCCTCTCCCACAGCAGGCAAAGC NGG 10 8 17 8 24 0 100 5 10
    260423212720_lbqk TAAGTGCTTTGCCTGCTGTGGGAGAGGGACTG(+CGTACGCATCGTCG)GATCCCGTCCTCTAACGTCTGTCTCTGGCCG NGG 10 8 17 8 24 0 100 5 10
    260423212706_pw84 GGACTACGCTGTGTCTGAGGCCTCGGCACATG(+CGTACGCATCGTCG)GTACCTGGGGGAGGGGACAAAGGGTGAGTAA NGG 10 8 17 8 24 0 100 5 10
    260423212644_tgaa GGCTCAGTTTCCCCGTGGGTGTCAGACAGTAG(+CGTACGCATCGTCG)GTACCACCTACTCCAGGGCCTTCACTAGGGC NGG 10 8 17 8 24 0 100 5 10
    260423212629_6a7m ATGAAGCCCTAGTGAAGGCCCTGGAGTAGGTG(+CGTACGCATCGTCG)GTACCTACTGTCTGACACCCACGGGGAAACT NGG 10 8 17 8 24 0 100 5 10
    260423212609_jef8 AGCCAGAGTCAAACTGATCCTTTATACAGGGG(+CGTACGCATCGTCG)GTACCATGACAGTACAACAGTGTCGTTAACC NGG 10 8 17 8 24 0 100 5 10
    260423212549_djgj GCAGTACTCTGAAGCAGGCAAATGGGTCTTAG(+CGTACGCATCGTCG)GATCCCTCCCAGAAACCTGCTCTGACCCAGA NGG 10 8 17 8 24 0 100 5 10