Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    241230210314_njww TCCTCCAAGGACAAGTCGATGGCGGCAGCGGCGGCGGGGGCAGGGGAGCAGGAGGAGGAGGTGGACGAGCTGCTGGCGGCGCTCGGGTACAAGGTGCGTTCGTCGGATATGGCGGACGTCGCGCAGAAGCTAGAGCAGCTCGAGATGGCCATGGGGATGGGCGGCGCCTG(/C)CCCCACCGCTGATGACGGGTTCGTCTCGCACCTCGCCACGGACACCGTGCACTACAATCCCTCCGACCTGTCGTCCTGGCTCGAGAGCATGCTG NGG 10 8 17 8 24 0 100 5 10
    241230203850_uy7j ACAGTTGCGCAGCCTGAATGGCGAATGGCGCTTTGCCTGGTTTCCGGCACCAGAAGCGGTGCCGGAAAGCTGGCTGGAGTGCGATCTTCCTGAGGCCGATACTGTCGTCGTCCCCTCAAACTGGCAGATGCACGGTTACGATGCGCCCATCTACACCAACGTGACCTATCCCATTACGGTCAATCCGCCGTTTGTTCCCACGGAGAATCCGACGGGTTGTTACTCGCTCACATTTAATGTTGATGAAAGCTGGCTACAGGAAG(-G)CCAGACGCGAATTATTTTTGATGGCGTTAACTCGGCGTTTCATCTGTGGTGCAACGGGCGCTGGGTCGGTTACGGCCAGGACAGTCGTTTGCCGTCTGAATTTGACCTGAGCGCATTTTTACGCGCCGGAGAAAACCGCCTCGCGGTGATGGTGCT NGG 10 8 17 8 24 0 100 5 10
    241230173043_3cij GGAGCGCCACGAGACGCTGGTCCAGTGGCGCGGCCGCCTCGGCGGGTCCGGGTTCGAGCCCGTGCACCTGGGATCCAACGCCTACAAGCAGGCAAGCACGCTGCTGGCCCTCTTCGCCGGCGGCGACGGGTACAGGGTGGAGGAGAAGGACGGGTGCCTGACTCTGGGATGGCATACGCGCCCGC(/T)TCATCGCCACCTCGGCGTGGCGCGTCGCCGCTCCGTGACGTGCCCACCGTCCGGCACAGCACAGCACAGCACAGCACAGTGGAAGCTAAGACGTCAGTGAACGCTGCTGAACTGCAG NGG 10 8 17 8 24 0 100 5 10
    241230171606_k6bb TCCTCCAAGGACAAGTCGATGGCGGCAGCGGCGGCGGGGGCAGGGGAGCAGGAGGAGGAGGTGGACGAGCTGCTGGCGGCGCTCGGGTACAAGGTGCGTTCGTCGGATATGGCGGACGTCGCGCAGAAGCTAGAGCAGCTCGAGATGGCCATGGGGATG(/C)GGCGGCGCCTGCCCCACCGCTGATGACGGGTTCGTCTCGCACCTCGCCACGGACACCGTGCACTACAATCCCTCCGACCTGTCGTCCTGGCTCGAGAGCATGCTG NGG 10 8 17 8 24 0 100 5 10
    241230171344_q6vc GGAGCGCCACGAGACGCTGGTCCAGTGGCGCGGCCGCCTCGGCGGGTCCGGGTTCGAGCCCGTGCACCTGGGATCCAACGCCTACAAGCAGGCAAGCACGCTGCTGGCCCTCTTCGCCGGCGGCGACGGGTACAGGGTGGAGGAGAAGGACGGGTGCCTGACTCTGGGATGGCATACGCGCCCGC(/T)TCATCGCCACCTCGGCGTGGCGCGTCGCCGCTCCGTGACGTGCCCACCGTCCGGCACAGCACAGCACAGCACAGCACAGTGGAAGCTAAGACGTCAGTGAACGCTGCTGAACTGCAG NGG 10 8 17 8 24 0 100 5 10
    241230165420_m11v CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    241230155636_9onk GGAGCGCCACGAGACGCTGGTCCAGTGGCGCGGCCGCCTCGGCGGGTCCGGGTTCGAGCCCGTGCACCTGGGATCCAACGCCTACAAGCAGGCAAGCACGCTGCTGGCCCTCTTCGCCGGCGGCGACGGGTACAGGGTGGAGGAGAAGGACGGGTGCCTGACTCTGGGATGGCATACGCGCCCGC(/T)TCATCGCCACCTCGGCGTGGCGCGTCGCCGCTCCGTGACGTGCCCACCGTCCGGCACAGCACAGCACAGCACAGCACAGTGGAAGCTAAGACGTCAGTGAACGCTGCTGAACTGCAG NGG 10 8 17 8 24 0 100 5 10
    241230154206_vxjh GGGTACAGGGTGGAGGAGAAGGACGGGTGCCTGACTCTGGGATGGCATACGCGCCCGC(/T)TCATCGCCACCTCGGCGTGGCGCGTCGCCGCTCCGTGACGTGCCCACCGTCCGGCA NGG 10 8 17 8 24 0 100 5 10
    241230153925_2j8z CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    241230092544_46aq GAGGTGGTGGGTGGGAAAGTCTTTTGTATCACTGTCTGTCTATCCATGCTCCACTCCTGTGTGTCTCCCTAAACTTGTTCTTTTCCTCTATTCCTAGGGAA(/C)TGGCCAAGAACGGCAGTGAAGCAGACATAGACGAGGGCCTTTACTCCCGGCAGCTGTAAGTGGGGCCAAGGCCGGGCTGAGGGGTGTGGAATGGGACATTGA NGG 10 8 17 8 24 0 100 5 10