Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250107180740_i58s GAAACTCATCAAATATGCGTGTTAGTGTAAATGAACTTCTATTTAATTTTGAGGCTCTGCAAAGTTCTTTGAAAGAGCAACAAAATGGCTTCAACTATCTGAGTGACACTGTGAAGGAGATGGCCAAGAAAGCACCTTCAGAAATATGCCAGAAATATCTGTCAGAATTTGAAGAGATTGAGGGGCACTGGAAGAAACTTTCCTCCC(+G)AGTTGGTGGAAAGCTGCCAAAAGCTAGAAGAACATATGAATAAACTTCGAAAATTTCAGGTAAGCCGAGGTTTGGCCTTTAAACTATATTTTTTCACATAGCAATTAATTGGAAAATGTGATGGGAAACAGATATTTTACCCAGAGTCCTTCAAAGATATTGATGATATCAAAAGCCAAATCTATTTCAAAGGATTGCAACTTGCCTATTTTTCCTATGAAAACAGTAATGTGTCATACCTTCTTGGATTGTCTGTATAAATGAATTGATTTT NGG 10 8 17 8 24 0 100 5 10
    250107180709_n80k GAAACTCATCAAATATGCGTGTTAGTGTAAATGAACTTCTATTTAATTTTGAGGCTCTGCAAAGTTCTTTGAAAGAGCAACAAAATGGCTTCAACTATCTGAGTGACACTGTGAAGGAGATGGCCAAGAAAGCACCTTCAGAAATATGCCAGAAATATCTGTCAGAATTTGAAGAGA(+C)TTGAGGGGCACTGGAAGAAACTTTCCTCCCAGTTGGTGGAAAGCTGCCAAAAGCTAGAAGAACATATGAATAAACTTCGAAAATTTCAGGTAAGCCGAGGTTTGGCCTTTAAACTATATTTTTTCACATAGCAATTAATTGGAAAATGTGATGGGAAACAGATATTTTACCCAGAGTCCTTCAAAGATATTGATGATATCAAAAGCCAAATCTATTTCAAAGGATTGCAACTTGCCTATTTTTCCTATGAAAACAGTAATGTGTCATACCTTCTTGGATTGTCTGTATAAATGAATTGATTTT NGG 10 8 17 8 24 0 100 5 10
    250107180605_96pm GAAACTCATCAAATATGCGTGTTAGTGTAAATGAACTTCTATTTAATTTTGAGGCTCTGCAAAGTTCTTTGAAAGAGCAACAAAATGGCTTCAACTATCTGAGTGACACTGTGAAGGAGATGGCCAAGAAAGCACCTTCAGAAATATGCCAGAAATATCTGTCAGAATTTGAAGAGATT(/A)GAGGGGCACTGGAAGAAACTTTCCTCCCAGTTGGTGGAAAGCTGCCAAAAGCTAGAAGAACATATGAATAAACTTCGAAAATTTCAGGTAAGCCGAGGTTTGGCCTTTAAACTATATTTTTTCACATAGCAATTAATTGGAAAATGTGATGGGAAACAGATATTTTACCCAGAGTCCTTCAAAGATATTGATGATATCAAAAGCCAAATCTATTTCAAAGGATTGCAACTTGCCTATTTTTCCTATGAAAACAGTAATGTGTCATACCTTCTTGGATTGTCTGTATAAATGAATTGATTTT NGG 10 8 17 8 24 0 100 5 10
    250107180519_fqke GAAACTCATCAAATATGCGTGTTAGTGTAAATGAACTTCTATTTAATTTTGAGGCTCTGCAAAGTTCTTTGAAAGAGCAACAAAATGGCTTCAACTATCTGAGTGACACTGTGAAGGAGATGGCCAAGAAAGCACCTTCAGAAATATGCCAGAAATATCTGTCAGAATTTGAAGAGATTGAGGGG(/A)CACTGGAAGAAACTTTCCTCCCAGTTGGTGGAAAGCTGCCAAAAGCTAGAAGAACATATGAATAAACTTCGAAAATTTCAGGTAAGCCGAGGTTTGGCCTTTAAACTATATTTTTTCACATAGCAATTAATTGGAAAATGTGATGGGAAACAGATATTTTACCCAGAGTCCTTCAAAGATATTGATGATATCAAAAGCCAAATCTATTTCAAAGGATTGCAACTTGCCTATTTTTCCTATGAAAACAGTAATGTGTCATACCTTCTTGGATTGTCTGTATAAATGAATTGATTTT NGG 10 8 17 8 24 0 100 5 10
    250107180447_lns1 GAAACTCATCAAATATGCGTGTTAGTGTAAATGAACTTCTATTTAATTTTGAGGCTCTGCAAAGTTCTTTGAAAGAGCAACAAAATGGCTTCAACTATCTGAGTGACACTGTGAAGGAGATGGCCAAGAAAGCACCTTCAGAAATATGCCAGAAATATCTGTCAGAATTTGAAGAGATTGAGGGGCACTGGAAGAAACTTTCCTCCCAGTTGGTGGAAAGCTGCCAAAA(/G)GCTAGAAGAACATATGAATAAACTTCGAAAATTTCAGGTAAGCCGAGGTTTGGCCTTTAAACTATATTTTTTCACATAGCAATTAATTGGAAAATGTGATGGGAAACAGATATTTTACCCAGAGTCCTTCAAAGATATTGATGATATCAAAAGCCAAATCTATTTCAAAGGATTGCAACTTGCCTATTTTTCCTATGAAAACAGTAATGTGTCATACCTTCTTGGATTGTCTGTATAAATGAATTGATTTT NGG 10 8 17 8 24 0 100 5 10
    250107180352_a5e1 GAAACTCATCAAATATGCGTGTTAGTGTAAATGAACTTCTATTTAATTTTGAGGCTCTGCAAAGTTCTTTGAAAGAGCAACAAAATGGCTTCAACTATCTGAGTGACACTGTGAAGGAGATGGCCAAGAAAGCACCTTCAGAAATATGCCAGAA(/G)ATATCTGTCAGAATTTGAAGAGATTGAGGGGCACTGGAAGAAACTTTCCTCCCAGTTGGTGGAAAGCTGCCAAAAGCTAGAAGAACATATGAATAAACTTCGAAAATTTCAGGTAAGCCGAGGTTTGGCCTTTAAACTATATTTTTTCACATAGCAATTAATTGGAAAATGTGATGGGAAACAGATATTTTACCCAGAGTCCTTCAAAGATATTGATGATATCAAAAGCCAAATCTATTTCAAAGGATTGCAACTTGCCTATTTTTCCTATGAAAACAGTAATGTGTCATACCTTCTTGGATTGTCTGTATAAATGAATTGATTTT NGG 10 8 17 8 24 0 100 5 10
    250107180245_kitv GAAACTCATCAAATATGCGTGTTAGTGTAAATGAACTTCTATTTAATTTTGAGGCTCTGCAAAGTTCTTTGAAAGAGCAACAAAATGGCTTCAACTATCTGAGTGACACTGTGAAGGAGATGGCCAAGAAAGCACCTTCAGAAATATGCCAGAAATATCTGTCAGAATTTGAAGAGA(+ctt)TTGAGGGGCACTGGAAGAAACTTTCCTCCCAGTTGGTGGAAAGCTGCCAAAAGCTAGAAGAACATATGAATAAACTTCGAAAATTTCAGGTAAGCCGAGGTTTGGCCTTTAAACTATATTTTTTCACATAGCAATTAATTGGAAAATGTGATGGGAAACAGATATTTTACCCAGAGTCCTTCAAAGATATTGATGATATCAAAAGCCAAATCTATTTCAAAGGATTGCAACTTGCCTATTTTTCCTATGAAAACAGTAATGTGTCATACCTTCTTGGATTGTCTGTATAAATGAATTGATTTT NGG 10 8 17 8 24 0 100 5 10
    250107180148_s5ok GAAACTCATCAAATATGCGTGTTAGTGTAAATGAACTTCTATTTAATTTTGAGGCTCTGCAAAGTTCTTTGAAAGAGCAACAAAATGGCTTCAACTATCTGAGTGACACTGTGAAGGAGATGGCCAAGAAAGCACCTTCAGAAATATGCCAGAAATATCTGTCAGAATTTGAAGAGATTGAGGGG(/A)CACTGGAAGAAACTTTCCTCCCAGTTGGTGGAAAGCTGCCAAAAGCTAGAAGAACATATGAATAAACTTCGAAAATTTCAGGTAAGCCGAGGTTTGGCCTTTAAACTATATTTTTTCACATAGCAATTAATTGGAAAATGTGATGGGAAACAGATATTTTACCCAGAGTCCTTCAAAGATATTGATGATATCAAAAGCCAAATCTATTTCAAAGGATTGCAACTTGCCTATTTTTCCTATGAAAACAGTAATGTGTCATACCTTCTTGGATTGTCTGTATAAATGAATTGATTTT NGG 10 8 17 8 24 0 100 5 10
    250107180118_wm8o GAAACTCATCAAATATGCGTGTTAGTGTAAATGAACTTCTATTTAATTTTGAGGCTCTGCAAAGTTCTTTGAAAGAGCAACAAAATGGCTTCAACTATCTGAGTGACACTGTGAAGGAGATGGCCAAGAAAGCACCTTCAGAAATATGCCAGAAATATCTGTCAGAATTTGAAGAGATTGAGGGG(/A)CACTGGAAGAAACTTTCCTCCCAGTTGGTGGAAAGCTGCCAAAAGCTAGAAGAACATATGAATAAACTTCGAAAATTTCAGGTAAGCCGAGGTTTGGCCTTTAAACTATATTTTTTCACATAGCAATTAATTGGAAAATGTGATGGGAAACAGATATTTTACCCAGAGTCCTTCAAAGATATTGATGATATCAAAAGCCAAATCTATTTCAAAGGATTGCAACTTGCCTATTTTTCCTATGAAAACAGTAATGTGTCATACCTTCTTGGATTGTCTGTATAAATGAATTGATTTT NGG 10 8 17 8 24 0 100 5 10
    250107180021_154l GAAACTCATCAAATATGCGTGTTAGTGTAAATGAACTTCTATTTAATTTTGAGGCTCTGCAAAGTTCTTTGAAAGAGCAACAAAATGGCTTCAACTATCTGAGTGACACTGTGAAGGAGATGGCCAAGAAAGCACCTTCAGAAATATGCCAGAAATATCTGTCAGAATTTGAAGAGATTGAGGGGCACTGGAAGAAACTTTCCTCCC(/G)AGTTGGTGGAAAGCTGCCAAAAGCTAGAAGAACATATGAATAAACTTCGAAAATTTCAGGTAAGCCGAGGTTTGGCCTTTAAACTATATTTTTTCACATAGCAATTAATTGGAAAATGTGATGGGAAACAGATATTTTACCCAGAGTCCTTCAAAGATATTGATGATATCAAAAGCCAAATCTATTTCAAAGGATTGCAACTTGCCTATTTTTCCTATGAAAACAGTAATGTGTCATACCTTCTTGGATTGTCTGTATAAATGAATTGATTTT NGG 10 8 17 8 24 0 100 5 10