Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    260424104348_a6c7 GGACTACGCTGTGTCTGAGGCCTCGGCACATG(+CGCGATGCGATGCA)GTACCTGGGGGAGGGGACAAAGGGTGAGTAA NGG 10 8 17 8 24 0 100 5 10
    260424104332_qj6r GGCTCAGTTTCCCCGTGGGTGTCAGACAGTAG(+CGCGATGCGATGCA)GTACCACCTACTCCAGGGCCTTCACTAGGGC NGG 10 8 17 8 24 0 100 5 10
    260424104317_xm12 ATGAAGCCCTAGTGAAGGCCCTGGAGTAGGTG(+CGCGATGCGATGCA)GTACCTACTGTCTGACACCCACGGGGAAACT NGG 10 8 17 8 24 0 100 5 10
    260424104259_zvmz AGCCAGAGTCAAACTGATCCTTTATACAGGGG(+CGCGATGCGATGCA)GTACCATGACAGTACAACAGTGTCGTTAACC NGG 10 8 17 8 24 0 100 5 10
    260424104246_ny8g GCAGTACTCTGAAGCAGGCAAATGGGTCTTAG(+CGCGATGCGATGCA)GATCCCTCCCAGAAACCTGCTCTGACCCAGA NGG 10 8 17 8 24 0 100 5 10
    260424104231_iw0x GTTAGGAGAGGTTAGGAAACCTCTAGAAAGTG(+CGCGATGCGATGCA)GATCCTTGCTGGGGGTAATTATATCACTGGG NGG 10 8 17 8 24 0 100 5 10
    260424104215_ka36 CCTACCCCAGTGATATAATTACCCCCAGCAAG(+CGCGATGCGATGCA)GATCCACTTTCTAGAGGTTTCCTAACCTCTC NGG 10 8 17 8 24 0 100 5 10
    260424104154_wth4 GCTACCCAGAGGCCTTTCGTCTCCCTGTTCTG(+CGCGATGCGATGCA)GTACCCAGCTTCCCAATCAGCAGCCAGAGCT NGG 10 8 17 8 24 0 100 5 10
    260424104136_nxio TTGTTCTAGAAGGCGCTCCTTTCAGGGTTGTG(+CGCGATGCGATGCA)GTACCTGTAGGTTAGCAGACCCTCCTCCTTT NGG 10 8 17 8 24 0 100 5 10
    260424104120_atrp GTGGGAAAGGAGGAGGGTCTGCTAACCTACAG(+CGCGATGCGATGCA)GTACCACAACCCTGAAAGGAGCGCCTTCTAG NGG 10 8 17 8 24 0 100 5 10