Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".
  • Substitution is indicated by backslash "/", and the example is used to replace A by T.
  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position
  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.
  • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250107191635_mwa6 AGAACGCTCTCAAGTCGTCCCCGCACGCTTCTCTCGGAGGTTTCCCGGTGGAGAAGTACATGGACGTGTCGGGCCTGGCGAGCAGCAGCGTACCGACCAACGAGGTGCCCGCAAGAGCCAAGGAAGTGTCCTTCTACCAGGGCTACACAAGTCCCTATCAGCACGTGCCTGGCTACATCGACATGGTGTCCACTTTTGGATCCGGGGAACCTCGGCACGAGGCATACATCTCCAT(/C)GGAGGGCTACCAGTCCTGGACGCTAGCCAACGGGTGGAACAGCCAGGTGTACTGTGCCAAGGATCAGCCACAGGGGTCCCATTTTTGGAAATCATCCTTTCCAGGTAGGAGCAACGAAAAAAGGGGAGGGAGAAAAAAGAAAAGCCCCTATTTGGGAGTGGTTACTTATATTCCAGGCCTGACCAGGTATAATCCACTAGAAAAATTTGTTTAGTCTCTGTGATCTCTGACTTTCCCAATTAAAA NGG 10 8 17 8 24 1 50 5 10
    250107191338_rje1 GCCAACCTTAGTAAATTCTTCCATGTGCTGTAGCTAAGAAACGACTTTCCTCTTGCAGATCGAGGAGACTGGCAGAGGGAAAGAAAGTTCAACTATGGTGGTGGTAACAGCGCCCCCTGGGGGGGCGACCGGCACCATCAGTATGAGCAGCATTGGT(/C)ATAAGGACCACCACTATGGTGACCGGAGGCATATGGATGCCCACCGTTCTGGGAGCTACCGCCCTAACAACATGTCCAGAAAGAGGCCGTATGAGCAGTACAACAGCGACCGAGACCACCGGGGACACAGAGACTATTATGACAGGTGTGTAGAAAGGTGTGAGAGGTGAGGCACTGACTTGTTCATGAGAAGAAGGATGGTGATGCCTATGCAGGCCTGTCCATCTGATGCATCCTGGGCTTCTCCCATGTGTCAGTTACAGATCCCTTGTCCCTGCTGTGGGGACAGCCTGAGGATGCAGGAGAGGAGGATGCATTGTCGG NGG 10 8 17 8 24 0 100 5 10
    250107191315_6r64 GCCAACCTTAGTAAATTCTTCCATGTGCTGTAGCTAAGAAACGACTTTCCTCTTGCAGATCGAGGAGACTGGCAGAGGGAAAGAAAGTTCAACTATGGTGGTGGTAACAGCGCCCCCTGGGGGGGCGACCGGCACCATCAGTATGAGCAGCATTGGTATAAGGACCACCACTATGGTGAC(/A)CGGAGGCATATGGATGCCCACCGTTCTGGGAGCTACCGCCCTAACAACATGTCCAGAAAGAGGCCGTATGAGCAGTACAACAGCGACCGAGACCACCGGGGACACAGAGACTATTATGACAGGTGTGTAGAAAGGTGTGAGAGGTGAGGCACTGACTTGTTCATGAGAAGAAGGATGGTGATGCCTATGCAGGCCTGTCCATCTGATGCATCCTGGGCTTCTCCCATGTGTCAGTTACAGATCCCTTGTCCCTGCTGTGGGGACAGCCTGAGGATGCAGGAGAGGAGGATGCATTGTCGG NGG 10 8 17 8 24 0 100 5 10
    250107191241_sh8a GCCAACCTTAGTAAATTCTTCCATGTGCTGTAGCTAAGAAACGACTTTCCTCTTGCAGATCGAGGAGACTGGCAGAGGGAAAGAAAGTTCAACTATGGTGGTGGTAACAGCGCCCCCTGGGGGGGCGACCGGCACCATCAGTATGAGCAGCATTGGTATAAGGACCACCA(/G)CTATGGTGACCGGAGGCATATGGATGCCCACCGTTCTGGGAGCTACCGCCCTAACAACATGTCCAGAAAGAGGCCGTATGAGCAGTACAACAGCGACCGAGACCACCGGGGACACAGAGACTATTATGACAGGTGTGTAGAAAGGTGTGAGAGGTGAGGCACTGACTTGTTCATGAGAAGAAGGATGGTGATGCCTATGCAGGCCTGTCCATCTGATGCATCCTGGGCTTCTCCCATGTGTCAGTTACAGATCCCTTGTCCCTGCTGTGGGGACAGCCTGAGGATGCAGGAGAGGAGGATGCATTGTCGG NGG 10 8 17 8 24 0 100 5 10
    250107190524_dehr CTCAGGTGTTCTCAGATGCTGAAGACTGGAGGGGTGTATTTTGAAGTATTGCTTTCTAAGATAAAAGTTTGGACACCAGTTTGAGGAATACATATACATTGATACCATTGCATTTCCTTTTCAAACACTAAAACTTGGTCTTCTTTTTATTCCTAGAAATGTCTCAGGCTGTGCAGAC(+C)AAATGGAACTCAACCATTAAGCAAAACATGGGAACTCAGTTTGTATGAGTTACAACGAACACCTCAGGTAATATAATACATCCTGCTGTAATTTAGGGAGATAGGCCTACATAAACTGGGTGTAGAAAAGGAGGACTTACATAGAACTTAGCAGTGATAGTAAACTTGCATAACAGTCCTGCGTCTTGTTTGGCTTACACTTCATTCATTAAGCAAAGTTACATTCTCTGTTTAATTCCATGAAAGCATCTAACTTTCAATATTTTAAATATATCATTTAAAAATCACCTTTGAATCTCAAA NGG 10 8 17 8 24 0 100 5 10
    250107190425_fodc CTCAGGTGTTCTCAGATGCTGAAGACTGGAGGGGTGTATTTTGAAGTATTGCTTTCTAAGATAAAAGTTTGGACACCAGTTTGAGGAATACATATACATTGATACCATTGCATTTCCTTTTCAAACACTAAAACTTGGTCTTCTTTTTATTCCTAGAAATGTCTCAGGCTGTGCAGACAAATGGAACTCAACCATTAAGCAAAA(/G)CATGGGAACTCAGTTTGTATGAGTTACAACGAACACCTCAGGTAATATAATACATCCTGCTGTAATTTAGGGAGATAGGCCTACATAAACTGGGTGTAGAAAAGGAGGACTTACATAGAACTTAGCAGTGATAGTAAACTTGCATAACAGTCCTGCGTCTTGTTTGGCTTACACTTCATTCATTAAGCAAAGTTACATTCTCTGTTTAATTCCATGAAAGCATCTAACTTTCAATATTTTAAATATATCATTTAAAAATCACCTTTGAATCTCAAA NGG 10 8 17 8 24 0 100 5 10
    250107182249_17tz AAATGTTCTTGGCTGTTTTGTATTGCCTTCTGTGGAGTTTCCAGATCTCTGATGGCCATTTTCCTCGAGCCTGTGCCTCCTCTAAGAACTTGTTGGCAAAAGAATGCTGCCCACCATGGATGGGTGATGGGAGTCCCTGCGGCCAGCTTTCAGGCAGAGGTTCCTGCCAGGATATCCTTCTGTCCAGTGCACCATCTGGACCTCAGTTCCCCTTCAAAGGGGTGGATGACCG(/T)TGAGTCCTGGCCCTCTGTGTTTTATAATAGGACCTGCCAGTGCTCAGGCAACTTCATGGGTTTCAACTGCGGAAACTGTAAGTTTGGATTTGGGGGCCCAAATTGTACAGAGAAGCGAGTCTTGATTAGAAGAAACATTTTTGATTTGAGTGTCTCCGAAAAGAATAAGTTCTTTTCTTACCTCACTTTAGCAAAACATACTATCAGCTCAGTCTATGTCATCCCCACAGGCACCTATGGCCAAATGA NGG 10 8 17 8 24 1 50 5 10
    250107182155_aqpa AAATGTTCTTGGCTGTTTTGTATTGCCTTCTGTGGAGTTTCCAGATCTCTGATGGCCATTTTCCTCGAGCCTGTGCCTCCTCTAAGAACTTGTTGGCAAAAGAATGCTGCCCACCATGGATGGGTGATGGGAGTCCCTGCGGCCAGCTTTCAGGCAGAGGTTCCTGCCAGGATATCCTTCTGTCCAGTGCACCATCTGGACCTCAGTTCCCCTTCAAAGGGGTGGATGACCG(/T)TGAGTCCTGGCCCTCTGTGTTTTATAATAGGACCTGCCAGTGCTCAGGCAACTTCATGGGTTTCAACTGCGGAAACTGTAAGTTTGGATTTGGGGGCCCAAATTGTACAGAGAAGCGAGTCTTGATTAGAAGAAACATTTTTGATTTGAGTGTCTCCGAAAAGAATAAGTTCTTTTCTTACCTCACTTTAGCAAAACATACTATCAGCTCAGTCTATGTCATCCCCACAGGCACCTATGGCCAAATGA NGG 10 8 17 8 24 0 100 5 10
    250107181905_fowv GAAACTCATCAAATATGCGTGTTAGTGTAAATGAACTTCTATTTAATTTTGAGGCTCTGCAAAGTTCTTTGAAAGAGCAACAAAATGGCTTCAACTATCTGAGTGACACTGTGAAGGAGATGGCCAAGAAAGCACCTTCAGAAATATGCCAGAAATATCTGTCAGAATTTGAAGAGATTGAGGGGCACTGGAAGAAACTTTC(/A)CTCCCAGTTGGTGGAAAGCTGCCAAAAGCTAGAAGAACATATGAATAAACTTCGAAAATTTCAGGTAAGCCGAGGTTTGGCCTTTAAACTATATTTTTTCACATAGCAATTAATTGGA NGG 10 8 17 8 24 1 50 5 10
    250107181746_5mm0 GAAACTCATCAAATATGCGTGTTAGTGTAAATGAACTTCTATTTAATTTTGAGGCTCTGCAAAGTTCTTTGAAAGAGCAACAAAATGGCTTCAACTATCTGAGTGACACTGTGAAGGAGATGGCCAAGAAAGCACCTTCAGAAATATGCCAGAAATATCTGTCAGAATTTGAAGAGATTGAGGGGCACTGGAAGAAACTTTC(/A)CTCCCAGTTGGTGGAAAGCTGCCAAAAGCTAGAAGAACATATGAATAAACTTCGAAAATTTCAGGTAAGCCGAGGTTTGGCCTTTAAACTATATTTTTTCACATAGCAATTAATTGGA NGG 10 8 17 8 24 0 100 5 10