Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    260424095757_vnya CTCCCCATGGATTCATGTGCACCCATCTGGAG(+TCAGCT)GTACCAGATTCCTCCATGGCAGATCTGTTGG NGG 10 8 17 8 24 0 100 5 10
    260424095729_yg3v TGTTCCCAACAGATCTGCCATGGAGGAATCTG(+TCAGCT)GTACCTCCAGATGGGTGCACATGAATCCATG NGG 10 8 17 8 24 0 100 5 10
    260424095712_we35 GACGCTTTTGTTAGAAACCTGAAGCCAGTTTG(+TCAGCT)GATCCTGGTGATCACTGTGTTATCAGTAGCA NGG 10 8 17 8 24 0 100 5 10
    260424095652_jma3 AAGTGTTGTTGGGCAGTCCCGTGTCATCCATG(+TCAGCT)GTACCTGCTTCAATTTCACACATAGCTGGTA NGG 10 8 17 8 24 0 100 5 10
    260424095618_d6r1 CAGTTTACCAGCTATGTGTGAAATTGAAGCAG(+TCAGCT)GTACCATGGATGACACGGGACTGCCCAACAA NGG 10 8 17 8 24 0 100 5 10
    260424095535_gvhq ATTAATTAGGGTGGCTTGATCAGCTACAGAAG(+TCAGCT)GATCCTTTGGGGTCACATTCTTTTATCCTGT NGG 10 8 17 8 24 0 100 5 10
    260424095118_rv4r GTGGGAAAGGAGGAGGGTCTGCTAACCTACAG(+TCAGCT)GTACCACAACCCTGAAAGGAGCGCCTTCTAG NGG 10 8 17 8 24 0 100 5 10
    260424095103_uzsb GTGGGAAAGGAGGAGGGTCTGCTAACCTACAG(+TCAGCT)GTACCACAACCCTGAAAGGAGCGCCTTCTAG NGG 10 8 17 8 24 0 100 5 10
    260424095045_a33o GTGGGAAAGGAGGAGGGTCTGCTAACCTACAG(+CGATTA)GTACCACAACCCTGAAAGGAGCGCCTTCTAG NGG 10 8 17 8 24 0 100 5 10
    260424095032_q1oo GTGGGAAAGGAGGAGGGTCTGCTAACCTACAG(+CGCGCC)GTACCACAACCCTGAAAGGAGCGCCTTCTAG NGG 10 8 17 8 24 0 100 5 10