Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250104110111_mazm AGTTGAGAACAAGTTTGGCAGCATCCGGCAGACATACACACTGGATGTGCTGGgtgagggccaggccgggtggctaaggcaaggggcatctctgggtcccttggccctactggccctgacctgactgcctcttgcagAGC(/T)GCTCCCCACACCGGCCCATCCTGCAGGCTGGGCTGCCGGCCAACCAGACAGCCATTCTAGGCAGTGACGTGGAG NGG 10 8 17 8 24 0 100 5 10
    250102071948_maj6 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250102070959_j8wf CAGCCCTTCCTGGACAAGAGCGGCCTGGAGAAGTATCTGTACCCGGCGGCGGCTGCCGCCCC(/G)GTTCCCGCTGCTATACCCCGGCATCCCCGCCCCGGCGGCAGCCGCGGCAGCCGCCGCCGCC NGG 10 8 17 8 24 0 100 5 10
    250102070730_8243 CAGCCCTTCCTGGACAAGAGCGGCCTGGAGAAGTATCTGTACCCGGCGGCGGCTGCCGCCCC(/G)GTTCCCGCTGCTATACCCCGGCATCCCCGCCCCGGCGGCAGCCGCGGCAGCCGCCGCCGCC NGG 10 8 17 8 24 0 100 5 10
    241231092519_2k4k TCCTCCAAGGACAAGTCGATGGCGGCAGCGGCGGCGGGGGCAGGGGAGCAGGAGGAGGAGGTGGACGAGCTGCTGGCGGCGCTCGGGTACAAGGTGCGTTCGTCGGATATGGCGGACGTCGCGCAGAAGCTAGAGCAGCCC(/G)GAGATGGCCATGGGGATGGGCGGCGCCTGCCCCACCGCTGATGACGGGTTCGTCTCGCACCTCGCCACGGACACCGTGCACTACAATCCCTCCGACCTGTCGTCCTGGCTCGAGAGCATGCTG NGG 10 8 17 8 24 0 100 5 10
    241230214224_sctb TCCTCCAAGGACAAGTCGATGGCGGCAGCGGCGGCGGGGGCAGGGGAGCAGGAGGAGGAGGTGGACGAGCTGCTGGCGGCGCTCGGGTACAAGGTGCGTTCGTCGGATATGGCGGACGTCGCGCAGAAGCTAGAGCAGCTCGAGATGGCCATGGGGATGGGCGGCGCCT(/A)GCCCCACCGCTGATGACGGGTTCGTCTCGCACCTCGCCACGGACACCGTGCACTACAATCCCTCCGACCTGTCGTCCTGGCTCGAGAGCATGCTG NGG 10 8 17 8 24 0 100 5 10
    241230214129_ftez TCCTCCAAGGACAAGTCGATGGCGGCAGCGGCGGCGGGGGCAGGGGAGCAGGAGGAGGAGGTGGACGAGCTGCTGGCGGCGCTCGGGTACAAGGTGCGTTCGTCGGATATGGCGGACGTCGCGCAGAAGCTAGAGCAGCTCGAGA(/T)TGGCCATGGGGATGGGCGGCGCCTGCCCCACCGCTGATGACGGGTTCGTCTCGCACCTCGCCACGGACACCGTGCACTACAATCCCTCCGACCTGTCGTCCTGGCTCGAGAGCATGCTG NGG 10 8 17 8 24 0 100 5 10
    241230214021_ufc6 TCCTCCAAGGACAAGTCGATGGCGGCAGCGGCGGCGGGGGCAGGGGAGCAGGAGGAGGAGGTGGACGAGCTGCTGGCGGCGCTCGGGTACAAGGTGCGTTCGTCGGATATGGCG(/C)GACGTCGCGCAGAAGCTAGAGCAGCTCGAGATGGCCATGGGGATGGGCGGCGCCTGCCCCACCGCTGATGACGGGTTCGTCTCGCACCTCGCCACGGACACCGTGCACTACAATCCCTCCGACCTGTCGTCCTGGCTCGAGAGCATGCTG NGG 10 8 17 8 24 0 100 5 10
    241230213836_h21k TCCTCCAAGGACAAGTCGATGGCGGCAGCGGCGGCGGGGGCAGGGGAGCAGGAGGAGGAGGTGGACGAGCTGCTGGCGGCGCTCGGGTACAAGGTGCGTTCGTCGGATATGGCGGACGTCGCGCAGAAGCTAGAGCAGCTCGA(/T)GATGGCCATGGGGATGGGCGGCGCCTGCCCCACCGCTGATGACGGGTTCGTCTCGCACCTCGCCACGGACACCGTGCACTACAATCCCTCCGACCTGTCGTCCTGGCTCGAGAGCATGCTG NGG 10 8 17 8 24 0 100 5 10
    241230213746_pj0l TCCTCCAAGGACAAGTCGATGGCGGCAGCGGCGGCGGGGGCAGGGGAGCAGGAGGAGGAGGTGGACGAGCTGCTGGCGGCGCTCGGGTACAAGGTGCGTTCGTCGGATATGGCGGACGTCGCGCAGAAGCTAGAGCAGCT(/C)GGAGATGGCCATGGGGATGGGCGGCGCCTGCCCCACCGCTGATGACGGGTTCGTCTCGCACCTCGCCACGGACACCGTGCACTACAATCCCTCCGACCTGTC NGG 10 8 17 8 24 0 100 5 10