Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".
  • Substitution is indicated by backslash "/", and the example is used to replace A by T.
  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position
  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.
  • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250203150823_lxrx TTCAAAAACCATTGGCTCACACTTTGCCTTTGGTCATTAATGTAAAGTCATCCACCATCACCAATTGCTTTGTGTTTATAGCAAAATATACAGGCAAATCAGATAGTAAGGTTACAAAGCAAAAGAATAATCCACCCTTCTGCTTCCTTCCAAGAAAAAGCTCAGAAAACAAAGCAAATAGAAACAGTTGCAACACTTAGCACATGTGACATGGTTAATATCTAACCTTTATC(+ctt)CACTGGAGATTTGTCTGTTTGAGCTTCTTTTCAAGTTTATCTTGCTCTTCTGGCCTTATGGGAGCACTTACAAGTACTGCTCCTCCTGTTTCATTTAATTGTTTTAGAATTCCCTGGCGCAGGGGCAACTCTTCTGCCAGTAACTGAAACGG NGG 10 8 17 8 24 0 100 5 10
    250203150747_0q02 TTCAAAAACCATTGGCTCACACTTTGCCTTTGGTCATTAATGTAAAGTCATCCACCATCACCAATTGCTTTGTGTTTATAGCAAAATATACAGGCAAATCAGATAGTAAGGTTACAAAGCAAAAGAATAATCCACCCTTCTGCTTCCTTCCAAGAAAAAGCTCAGAAAACAAAGCAAATAGAAACAGTTGCAACACTTAGCACATGTGACATGGTTAATATCTAACCTTTATC(+A)CACTGGAGATTTGTCTGTTTGAGCTTCTTTTCAAGTTTATCTTGCTCTTCTGGCCTTATGGGAGCACTTACAAGTACTGCTCCTCCTGTTTCATTTAATTGTTTTAGAATTCCCTGGCGCAGGGGCAACTCTTCTGCCAGTAACTGAAACGG NGG 10 8 17 8 24 0 100 5 10
    250131055041_fsdg GGCCCTTTTCCAGGTTAGATGGTTGCTAACCTCGGAGCAGTCTATTTTCCCGCCTTTTCCGCGCCCAGCAGCAGCTTCCTTTGCCCCTCCCAACTTCCAC(/G)CCTGCGCGCAGTCGCAGATCGCCGCTGCTGTCCGTCCACCCGCGCCCCCTCAAAGCTTCCCCTAGCAACAAGCTCGGGCTCAGAGCGAAGGCGGGGCGAG NGG 10 8 17 8 24 0 100 5 10
    250131055024_pmxs GGCCCTTTTCCAGGTTAGATGGTTGCTAACCTCGGAGCAGTCTATTTTCCCGCCTTTTCCGCGCCCAGCAGCAGCTTCCTTTGCCCCTCCCAACTTCCAC(/G)CCTGCGCGCAGTCGCAGATCGCCGCTGCTGTCCGTCCACCCGCGCCCCCTCAAAGCTTCCCCTAGCAACAAGCTCGGGCTCAGAGCGAAGGCGGGGCGAG NGG 10 8 17 8 24 0 100 5 10
    250130044215_72pc GGCCCTTTTCCAGGTTAGATGGTTGCTAACCTCGGAGCAGTCTATTTTCCCGCCTTTTCCGCGCCCAGCAGCAGCTTCCTTTGCCCCTCCCAACTTCCAC(/G)CCTGCGCGCAGTCGCAGATCGCCGCTGCTGTCCGTCCACCCGCGCCCCCTCAAAGCTTCCCCTAGCAACAAGCTCGGGCTCAGAGCGAAGGCGGGGCGAG NGG 10 8 17 8 24 0 100 5 10
    250126180303_sdkp tgatattttttcttgaaacaaaacttttcatggtgggcgtgaataaccatttcacaaagtgcaaggg(+CTTCCAGAAC)tagttttaaacttttttatcgttttttttttcccctcttggagtaataatttgttcggtatacttcagttcatac NGG 10 8 17 8 24 0 100 5 10
    250126174049_z74g tgatattttttcttgaaacaaaacttttcatggtgggcgtgaataaccatttcacaaagtgcaaggg(+CTTCCAGAACCT)tagttttaaacttttttatcgttttttttttcccctcttggagtaataatttgttcggtatacttcagttcatac NGG 10 8 17 8 24 0 100 5 10
    250126173636_tjxf tgatattttttcttgaaacaaaacttttcatggtgggcgtgaataaccatttcacaaagtgcaaggg(+CTTCCAGAACC)tagttttaaacttttttatcgttttttttttcccctcttggagtaataatttgttcggtatacttcagttcatac NGG 10 8 17 8 24 0 100 5 10
    250126173236_ph3d tgatattttttcttgaaacaaaacttttcatggtgggcgtgaataaccatttcacaaagtgcaaggg(+CTTCCAGAAC)tagttttaaacttttttatcgttttttttttcccctcttggagtaataatttgttcggtatacttcagttcatac NGG 10 8 17 8 24 0 100 5 10
    250126173200_ogw5 tgatattttttcttgaaacaaaacttttcatggtgggcgtgaataaccatttcacaaagtgcaaggg(+CTT)tagttttaaacttttttatcgttttttttttcccctcttggagtaataatttgttcggtatacttcagttcatac NGG 10 8 17 8 24 0 100 5 10