Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    260424131907_zk3c GTTAGGAGAGGTTAGGAAACCTCTAGAAAGTG(+CGACTACATAGACG)GATCCTTGCTGGGGGTAATTATATCACTGGG NGG 6 8 17 8 24 0 100 5 10
    260424131844_7c7k CCTACCCCAGTGATATAATTACCCCCAGCAAG(+CGACTACATAGACG)GATCCACTTTCTAGAGGTTTCCTAACCTCTC NGG 6 8 17 8 24 0 100 5 10
    260424131816_qjcp GCTACCCAGAGGCCTTTCGTCTCCCTGTTCTG(+CGACTACATAGACG)GTACCCAGCTTCCCAATCAGCAGCCAGAGCT NGG 6 8 17 8 24 0 100 5 10
    260424131803_juk0 TTGTTCTAGAAGGCGCTCCTTTCAGGGTTGTG(+CGACTACATAGACG)GTACCTGTAGGTTAGCAGACCCTCCTCCTTT NGG 6 8 17 8 24 0 100 5 10
    260424131750_pmog GTGGGAAAGGAGGAGGGTCTGCTAACCTACAG(+CGACTACATAGACG)GTACCACAACCCTGAAAGGAGCGCCTTCTAG NGG 6 8 17 8 24 0 100 5 10
    260424131733_2tzy GTGTAGTGTTCCTAGATTCAGCTGAAGACCTG(+CGACTACATAGACG)GTACCTAGGGTTCCTAACGGGATGGTGTTAG NGG 6 8 17 8 24 0 100 5 10
    260424131719_emxs CAAAACCATCTGTAGCTCCTCCACTTTCAGGG(+CGACTACATAGACG)GATCCGGCACCCTCTGCAGGCACTAGGAGCA NGG 6 8 17 8 24 0 100 5 10
    260424131703_zk1h ACTTCTGCTCCTAGTGCCTGCAGAGGGTGCCG(+CGACTACATAGACG)GATCCCCTGAAAGTGGAGGAGCTACAGATGG NGG 6 8 17 8 24 0 100 5 10
    260424131650_mfvm TGCATTTTCCTGACTGATGATTGATGTGGGAG(+CGACTACATAGACG)GATCCATCCCAATGTGGGCAACTATAAGAAA NGG 6 8 17 8 24 0 100 5 10
    260424131626_89c2 CCTGCTTTCTTATAGTTGCCCACATTGGGATG(+CGACTACATAGACG)GATCCTCCCACATCAATCATCAGTCAGGAAA NGG 6 8 17 8 24 0 100 5 10