Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250610163638_iql06e7x TGGAATACTCCCCTGCTACCGGTTAGGAAGCCTGGGACCAATGATTAT(+TAGTGA)CGACCAGTACAGGACTTGAGAGAGGTCAATAAAAGGGTGCAGGACATACACCCAACGGTCCCGAACCCTTATAACCTCTTGAGCGCTCTCCCGCCCGAACGGAACTGGTACACAGTATTGGACTTAAAAGATGCCTTCTTCTGCCTGAGATTACACTCCCACTAGCCAACCACTTTTTGCCTTCGAATGGAGAGATCCAGGTACGGGAAGAACCGGGCAGCTCACCTGGACCCGACTGCCCCAA NGG 10 8 17 8 24 0 100 5 10
    250610163638_iql06cf7 TGGAATACTCCCCTGCTACCGGTTAGGAAGCCTGGGACCAATGATTATCGACCAGTACAGGACTTGAGAGAGGTCAATAAAAGGGTGCAGGACATACACCCAACGGTCCCGAACCCTTATAACCTCTTGAGCGCTCTCCCGCCCGAACGGAACTGGTACACAGTATTGGACTTAAAAGATGCCTTCTTC(+TAGTGA)TGCCTGAGATTACACTCCCACTAGCCAACCACTTTTTGCCTTCGAATGGAGAGATCCAGGTACGGGAAGAACCGGGCAGCTCACCTGGACCCGACTGCCCCAA NGG 10 8 17 8 24 0 100 5 10
    250610163638_iql05gk7 TGGAATACTCCCCTGCTACCGGTTAGGAAGCCTGGGACCAATGATTATCGACCAGTACAGGACTTGAGAGAGGTCAATAAAAGGGTGCAGGACATACACCCAACGGTCCCGAACCCTTATAACCTCTTGAGCGCTCTCCCGCCCGAACGGAACTGGTACACAGTATTGGACTTAAAAGATGCCTTCTTCTGCCTGAGATTACACTCCCACTAGCCAACCACTTTTTGCCTT(+TAGTGA)CGAATGGAGAGATCCAGGTACGGGAAGAACCGGGCAGCTCACCTGGACCCGACTGCCCCAA NGG 10 8 17 8 24 0 100 5 10
    250610163638_iql04w9y TGGAATACTCCCCTGCTACCGGTTAGGAAGCCTGGGACCAATGATTATCGACCAGTACAGGACTTGAGAGAGGTCAATAAAAGGGTGCAGGACATACACCCAACGGTCCCGAACCCTTATAACCTCTTGAGCGCTCTCCCGCCCGAACGGAACTGGTACACAGTATTGGACTTAAAAGATGCCTTCTTCTGCCTGAGATTACACTCCCACTAGCCAACCACTTTTTGCCTTCGAATGGAGAGATCCAGG(+TAGTGA)TACGGGAAGAACCGGGCAGCTCACCTGGACCCGACTGCCCCAA NGG 10 8 17 8 24 0 100 5 10
    250610163638_iql04vn9 TGGAATACTCCCCTGCTACCGGTTAGGAAGCCTGGGACCAATGATTATCGACCAGTACAGGACTTGAGAGAGGTCAATAAAAGGGTGCAGGACATACACCCAACGGTCCCGAACCCTTATAACCTCTTGAGCGCTCTCCCGCCCGAACGGAACTGGTACACAGTATTGGACTTAAAAGATGCCTTCTTCTGCCTGAGATTACACTCCCACTAGCCAACCACTTTTTGCCTTCGAATGGAGAGATCCAGGTACGGGAAGAAC(+TAGTGA)CGGGCAGCTCACCTGGACCCGACTGCCCCAA NGG 10 8 17 8 24 0 100 5 10
    250610163638_iql04u9i TGGAATACTCCCCTGCTACCGGTTAGGAAGCCTGGGACCAATGATTATCGACCAGTACAGGACTTGAGAGAGGTCAATAAAAGGGTGCAGGACATACACCCAACGGTCCCGAACCCTTATAACCTCTTGAGCGCTCTCCCGCCCGAACGGAACTGGTACACAGTATTGGACTTAAAAGATGCC(+TAGTGA)TTCTTCTGCCTGAGATTACACTCCCACTAGCCAACCACTTTTTGCCTTCGAATGGAGAGATCCAGGTACGGGAAGAACCGGGCAGCTCACCTGGACCCGACTGCCCCAA NGG 10 8 17 8 24 0 100 5 10
    250610163638_iql04qe1 TGGAATACTCCCCTGCTACCGGTTAGGAAGCCTGGGACCAATGATTATCGACCAGTACAGGACTTGAGAGAGGTCAATAAAAGG(+TAGTGA)GTGCAGGACATACACCCAACGGTCCCGAACCCTTATAACCTCTTGAGCGCTCTCCCGCCCGAACGGAACTGGTACACAGTATTGGACTTAAAAGATGCCTTCTTCTGCCTGAGATTACACTCCCACTAGCCAACCACTTTTTGCCTTCGAATGGAGAGATCCAGGTACGGGAAGAACCGGGCAGCTCACCTGGACCCGACTGCCCCAA NGG 10 8 17 8 24 0 100 5 10
    250610163638_iql03rwi TGGAATACTCCCCTGCTACCGGTTAGGAAGCCTGGGACCAATGATTATCGACCAGTACAGGACTTGAGA(+TAGTGA)GAGGTCAATAAAAGGGTGCAGGACATACACCCAACGGTCCCGAACCCTTATAACCTCTTGAGCGCTCTCCCGCCCGAACGGAACTGGTACACAGTATTGGACTTAAAAGATGCCTTCTTCTGCCTGAGATTACACTCCCACTAGCCAACCACTTTTTGCCTTCGAATGGAGAGATCCAGGTACGGGAAGAACCGGGCAGCTCACCTGGACCCGACTGCCCCAA NGG 10 8 17 8 24 0 100 5 10
    250610163638_iql02ld6 TGGAATACTCCCCTGCTACCGGTTAGGAAGCCTGGGACCAATGATTATCGACCAGTACAGGACTTGAGAGAGGTCAATAAAAGGGTGCAGGACATACACCCAACGGTCCCGAACCCTTATAACCTCTTGAGCGCTCTCCCGCCCGAACGGAACTGGTACACAGTATTGGACTTAAAAGATGCCTTCTTCTGCCTGAGATTACACTCCCACTAGCCAACCACTTTTTGCCTTCGAATGGAGAGATCCAGGTACGGGAAGAACCGGGCA(+TAGTGA)GCTCACCTGGACCCGACTGCCCCAA NGG 10 8 17 8 24 0 100 5 10
    250610163638_iql024ih TGGAATACTCCCCTGCTACCGGTTAGGAAGCCTGGGACCAATGATTATCGACCAGTACAGGACTTGAGAGAGGTCAATAAAAGGGTGCAGGACATACACCCAACGGTCCCGAACCCTTATAACCTCTTGAGCGCTCTCCCGCCCGAACGGAACTGGTACACAGTATTGGACTTAAAAGATGCCTTC(+TAGTGA)TTCTGCCTGAGATTACACTCCCACTAGCCAACCACTTTTTGCCTTCGAATGGAGAGATCCAGGTACGGGAAGAACCGGGCAGCTCACCTGGACCCGACTGCCCCAA NGG 10 8 17 8 24 0 100 5 10