Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    260424102840_oaeq AGGCCTAGGACCAAGGTATCCTCTGTCTGCAG(+CGTATC)GTACCTGACTACCCACAACAGGGAGGCTCGC NGG 10 8 17 8 24 0 100 5 10
    260424102826_i0np AGATGGCGAGCCTCCCTGTTGTGGGTAGTCAG(+CGTATC)GTACCTGCAGACAGAGGATACCTTGGTCCTA NGG 10 8 17 8 24 0 100 5 10
    260424102813_jxx7 TGCAGAGATGGTTTATGAGGCGGGTCCGAAGG(+CGTATC)GTACCCTTCACCTTGCCTGGAGATATACAAA NGG 10 8 17 8 24 0 100 5 10
    260424102800_1l6g TCTGTGCTCAGGTCACACAGGGGGCTTCAGAG(+CGTATC)GATCCTTATGAAAGATGAGTTAAAAAATGTC NGG 10 8 17 8 24 0 100 5 10
    260424102747_dfob GAAGAGAGACAAGGAGAAGCAGAAAGCAGAAG(+CGTATC)GTACCCTTTTATTGGCATTTTAACAATGGCA NGG 10 8 17 8 24 0 100 5 10
    260424102731_l7fe AGAATCGCTTAAGCAGGGCTGTGGGCCTGCAG(+CGTATC)GATCCCCAGGTCCCTTTGGACCGGAGGGGGA NGG 10 8 17 8 24 0 100 5 10
    260424102717_5qwj GACTTTATGTGGGAGCTGGGGATTTGAACTAG(+CGTATC)GATCCTTGTATAGCAAGCATCCATATTCTGA NGG 10 8 17 8 24 0 100 5 10
    260424102701_vzz4 ATGGTTCAGAATATGGATGCTTGCTATACAAG(+CGTATC)GATCCTAGTTCAAATCCCCAGCTCCCACATA NGG 10 8 17 8 24 0 100 5 10
    260424102645_udjs GACCCACAGGAACTATGAAGGAAGAAGCGTAG(+CGTATC)GATCCCAGAGCAATCATATAACCGAACTCTG NGG 10 8 17 8 24 0 100 5 10
    260424102628_luht GTAGCCTTTGCGATTGTGTGATCCAAGAGCTG(+CGTATC)GATCCATACAATTTGTGCCACCCTGAAATTG NGG 10 8 17 8 24 0 100 5 10