Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250121051821_gprb CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250119205136_7em5 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250118222146_bwml CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250118084030_rqvo AGTTGAGAACAAGTTTGGCAGCATCCGGCAGACATACACACTGGATGTGCTGGgtgagggccaggccgggtggctaaggcaaggggcatctctgggtcccttggccctactggccctgacctgactgcctcttgcagAGCGCTC(/G)CCCACACCGGCCCATCCTGCAGGCTGGGCTGCCGGCCAACCAGACAGCCATTCTAGGCAGTGACGTGGAG NGG 10 8 17 8 24 0 100 5 10
    250118084009_nya0 AGTTGAGAACAAGTTTGGCAGCATCCGGCAGACATACACACTGGATGTGCTGGgtgagggccaggccgggtggctaaggcaaggggcatctctgggtcccttggccctactggccctgacctgactgcctcttgcagAGCGCTC(/G)CCCACACCGGCCCATCCTGCAGGCTGGGCTGCCGGCCAACCAGACAGCCATTCTAGGCAGTGACGTGGAG NGG 10 8 17 8 24 0 100 5 10
    250118074549_p28z AGTTGAGAACAAGTTTGGCAGCATCCGGCAGACATACACACTGGATGTGCTGGgtgagggccaggccgggtggctaaggcaaggggcatctctgggtcccttggccctactggccctgacctgactgcctcttgcagAGC(/T)GCTCCCCACACCGGCCCATCCTGCAGGCTGGGCTGCCGGCCAACCAGACAGCCATTCTAGGCAGTGACGTGGAG NGG 10 8 17 8 24 0 100 5 10
    250114205420_1fjn cgacgagcaggccgacttcgtctgcaacaccctgcagcccggctgcaagaacgtctgctacgaccactacttccccatctcgcacatccggctctgggcg(-c)tgcagctcatcttcgtgtccacgcccgccctgctggtggccatgcacgtggcctaccggcgacacgagaagaaaaggaagttcatcaagggcgagatgaa NGG 10 8 17 8 24 0 100 5 10
    250114200700_c5oo AGTTGATAAGCGAACCTCACACAACAGCTTCATGTCAGCCAAGGCCACAAACACCATGTACCACACATGTGAACGGACAGATTGACATGTTAAAAACACAACATCAGTGCATGTTGGGGATTCCTGGTGCCAGAAACAGGGGT(+C)GACGGGAGGGCAGAACTAGTCCTTAGCAGCTTCCTCCTCCTTTATTTTAGCTGAAGGGAAATAAAAGGAAAAGTCACTCTGGGGAACACGCCCGGTGTCACGCCACTTGACAGGCGAGTAACAGACATGGACCATCAGGAAACATTAACGTACTGATGTTAACAGCTGACCCAATAAGTGGCAGAGTGCTAAGGGAACGTTCACGGAGACTGAACACTCCTCAAACGGTCCCCAGAGGGTTCTAGACCCAGAGGCTCAAGTGAGCAGCTGAGGCAGGTGCCTGCTGAGCCAAATTCACCGAGCAGGAGTGAGGGAAACGGCCCCAGGGCCAGCCCAG NGG 10 8 17 8 24 0 100 5 10
    250114200405_wdh7 GGCTCAGTCTGAAGAGCAGAGCCAGGAACCCCTGTAGGGAAGGGGCAGGAGAGCCAGGGGCATGAGATGGTGGACGAGGAAGGGGGACAGGGAAGCCTGAGCGCCTCTCCTGGGCTTGCCAAGGACTCAAACCCAGAAGCCCAGAGCAGGGCCTTAGGGAAGCGGGACCCTGCTCTGGGCGGAGGAATATGTCCCAGATAGCACTGGGGACTCTTTAAGGAAAGAAGGATGGAGAAAGAGAAAGGGAGTAGAGGCGGCCACGACCTGGTGAACACCTAGGACGCACCATTCTCACAAAGGGAGTTTTCCACACGGACACCCCCCTCCTCACCACAGCCCTGCCAGGACGGGGCTGGCTACTGGCCTTATCTCACAGGTAAAACTGACGCA(+T)CGGAGGAACAATATAAATTGGGGACTAGAAAGGTGAAGAGCCAAAGTTAGAACTCAGGACCAACTTATTCTGATTTTGTTTTTCCAAACTGCTTCTCCTCTTGGGAAGTGTAAGGAAGCTGCAGCACCAGGATCAGTGAAACGCACCAGACAGCCGCGTCAGAGCAGCTCAGGTTCTGGGAGAGGGTAGCGCAGGGTGGCCACTGAGAACCGGGCAGGTCACGCATCCCCCCCTTCCCTCCCACCCCCTGCCAAGCTCTCCCTCCCAGGATCCTCTCTGGCTCCATCGTAAGCAAACCTTAGAGGTTCTGGCAAGGAGAGAGATGGCTCCAGGAAATGGGGGTGTGTCACCAGATAAGGAATCTGCCTAACAGGAGGTGGGGGTTAGACCCAATATCAGGAGACTAGGAAGGAGGAGGCCTAAGGATGGGGCTTTTCTGTCACCAATCCTGTCCCTAGTGGCCCCACTGTGGGGTGGAGGGGACAGATAAAAGTACCCAGAACCAGAGCCACATTAACCGGCCCTGGGAATATAAGGTGGTCCCAGCTCGGGGACACAGGATCCCTGGAG NGG 10 8 17 8 24 0 100 5 10
    250114200226_u5aq AGCTGACCCCGCCCACCCACTCATTCACCTACCAGTGCTCCAGTCATGCAACCCTGACACCCTTCCGCTAAATCGAGGCCTACAGAACCCCAACAGGCACCCAGGATGACTGCTGGATGACCAGTTGAGAAAGATGTGCCACTAGAGTTCTCAGCCTCCCGATGCCACTTTACTTCGGAGGACACGTTTTCTGCATGACCACACTGGTAGCCCGCTCCCCGCCTCACACTTTTGGAACCAAGCCCCGCAGTGCCTTTAGCACGTGGCCTCCCCCATCACTCTGTGCCACAAGGTGCAGCTGAGCACTAAGTCTTGCCCTCGCCTCCCGCACCTCAGGGCTGCACTTAGAGACCACCAGACGGCTAGATGAGCAGAGAAGACCCCCGAAGAGCATCACCCCAACCCCAAAGCAACGCCGTTGCCTGGCACCCATACCTTGGAGCAA(+T)CGGCGGAAGGTGGCGGTGGCCACATGTGCGGCCTCATCAGCCAGGCCATCCTCCTGGGACGGGAGGGCGAGCATCAGCTCTTCATAATCTCCATCCTCGTCCTGGGCACCAGCGCCGGTGGGGCACAGTAGCAGCAACAGCAGCAGCAGCGGCGGCAACAGCGGCAACAGCGGCCACCGCAGCCACGCAGAGCAGTGGGTGCCCATCGGGGCGAGGAGAGGTGCGCGCGGCCTCGGGAAGGACATGGACGCCTGGGACTCGGGGCACTTGGCTCCGGGGCTGCGGGCCGTGATGTCTCTGGGGAGCCAAGGACTGGCTCAGTCCTCTAGCCTCAGAAAGAGAGAGAGAACGGGAG NGG 10 8 17 8 24 0 100 5 10