Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250310014327_22u0 TAACTTACAACTTACATGAAAGAAGAGTGGAG(+ACTCAC)GATCCTGTTGGATTGTTTGGCTGGACACTAT NGG 10 8 17 8 24 0 100 5 10
    250307175540_8szy GGCTCTGCAAAGTTCTTTGAAAGAGCAACAAAATGGCTTCAACTATCTGAGTGACACTGTGAAGGAGATGGCCAAGAAAGCACCTTCAGAAATATGCCAGAAATATCTGTCAGAATTTGAAGAGA(+a)TTGAGGGGCACTGGAAGAAACTTTccTCCCAGTTGGTGGAAAGCTGCCAAAAGCTAGAAGAACATATGAATAAACTTCGAAAATTTCAGGTAAGCCGAGGTTTGGCCTTTAAACTATATTTTTTCACATAGCAATTAATTGGA NGG 10 8 17 8 24 0 100 5 10
    250307175509_n1rf GCTCTGCAAAGTTCTTTGAAAGAGCAACAAAATGGCTTCAACTATCTGAGTGACACTGTGAAGGAGATGGCCAAGAAAGCACCTTCAGAAATATGCCAGAAATATCTGTCAGAATTTGAAGAGA(+ctt)TTGAGGGGCACTGGAAGAAACTTTccTCCCAGTTGGTGGAAAGCTGCCAAAAGCTAGAAGAACATATGAATAAACTTCGAAAATTTCAGGTAAGCCGAGGTTTGGCCTTTAAACTATATTTTTTCACATAGCAATTAATTGGA NGG 10 8 17 8 24 0 100 5 10
    250306193916_oyzn attaagtagccctgcatttcaggtttccttgagtggcaggccaggcctggccgtgaacgttcactgaaatcatggcctcttggccaagattgatagcttgtgcctgtccctgagtcccagtccatcacgagcagctggtttctaagatgctatttcccgtataaagcatgagaccgtgacttgccagccccacagagccccgcccttgtccatcactggcatctggactccagcctgggttggggcaaagagggaaatgagatcatgtcctaaccctgatcctcttgtcccacagatatccagaaccctgaccctgccgtgtACC(/T)agctgagagactctaaatccagtgacaagtctgtctgcctattcaccgattttgattctcaaacaaatgtgtcacaaagtaaggattctgatgtgtatatcacagacaaaactgtgctagacatgaggtctatggacttcaagagcaacagtgctgtggcctggagcaacaaatctgactttgcatgtgcaaacgccttcaacaacagcattattccagaagacaccttcttccccagcccaggtaagggcagctttggtgccttcgcaggctgtttccttgcttcaggaatggccaggttctgcccagagctctggtcaatgatgtctaaaactcctctgattggtggtctcggccttatccattgccaccaaa NGG 10 8 17 8 24 0 100 5 10
    250306190442_c7dj CGACACCAAACATGGCTTGTCCAGCCTGGCTACGAGGACCAATTATACGCAGTTTTGGCGTGGAAAATTCTCAGCTGGGTCATGTCTG(+ATCCGAAATA)TGGCCCACATCCATATATGTTTGGAAGCAAAGAAAATGAAGGAAAAATAACCAGAGAAATAAGAAAGTGTATTATTTAAAACAGGGAATTCGGACTCTTTCT NGG 10 8 17 8 24 0 100 5 10
    250306182658_zehl CGAAACCCGCCCGGGGCGCACGGAGGACGGCCCCAGTCCAGCTCGTCCGGCTTCCCACCCGCAGGCGGACCCCGAAGCCGCGCCCGCGCCCGCCCGCGGCGACTCCGGCCCGGGCACGCGCACGAGTCGGAAGGGGGAGGGGCGGGAGGAGGTGGGGGAGGGGCGAGCGGGCCGAGGCCCCGCCCCCGCTCCTCTGCCCCGCCCGCCGCGATTATAGCCGATGACTCAGGGCGGAGCTCCGCATCCAACCCCGGGCCGCGGCCAAC(/T)TTCTCTGGACTGGACCAGAAGTTTCTAGCCGGCCAGTTGCTACCTCCCTTTATCTCCTCCTTCCCCTCTGGCAGCGAGGAGGCTATTTCCAGACACTTCCACCCCTCTCTGGCCACGTCACCCCCGCCTTTAATTCATAAAGGTGCCCGGCGCCGGCTTCCCGGACACGTCGGCGGCGGAGAGGGGCCCACGGCGGCGGCCCGGCCAGAGACTC NGG 10 8 17 8 24 0 100 5 10
    250306180306_2g8v tggacccccaggctggaggccagctctccaccctccctcagcctcccacctcccagtggctgccttgggtggaggggctgaccggtgactgtccctccagctgctgtggcaccgcgcccagtatgagccgctcttccacatgctcagtggccccgaggcctatgtgttcacctgcatcaaccagacagcggagcagcaaG(/A)agctggaggacgagcaacggcgtctgtgtgacgtgcagcccttcctgcccgtcctgcgcctggtggcccgtgagggcgaccgcgtgaagaagctcatcaactcaTagatcagcctcctcatcggcaaaggtagctctgccgagtgggccgtgtggccgggctggccctgcctgccccacccgctgacccagccctccccaccccgcaggcctccacgagt NGG 100 6 20 8 24 0 100 5 10
    250304004514_qn3e aagagcttctgttttggcacacgggttcttcaccccatccctttctcctcgccagcccaaactcactgcagtcgctaaggcttggatcaagcctcaaaccagaagcttgcatcctagcctgctctctctgaggtgaggttagagctggaggactgacggctactaactgccttacaggtgaaatcgccctgtggtccctggtggtcctggccattgagcgctacgtggtggtctgcaagccgatgagcaacttccgcttcggggagaatcacgctatcatgggtgtggtcttcacctggatcatggcgttggcctgtgctgctcccccactcgttggctggtccaggtaatggcactgagtatcgggtctggcaaggtctttgtgggattccctttgaggacacagagccctcggattggttccaggcataatgtaacatggtattgccccccgaaaaccatcctggtgactttcccaggctaaggtctaaggtaggggagaagagagggactgaatggtccaatcagtcttattccatgtctgagacccataacaaggagaaccctggacattccaacccttcaccttggccgagtccctaatcctcggctaagccaaggccaaaccacaatcctctttggttgagttctggccgtgggcctctctctctcttcctctctctctctcactcaccttggaccttagcccctggagaggctgaaccttcccaaaatgcatggtgacattgtagccccaggaactgggtcccatccagcctccaggccaccatatctaaatgagacaagagaaggttgggacagtggtttggacacctagacaggctatgaggtacacagagcctcagagactctccattctctgtcctcatgtcctccccaccccgggagcccacccagtggccctcactgagtcagagcccctcactcctcactggcctctttcctcatcctcacccacctggcttggcaggcctctacagacacactcagtggacacttgggtttctgagtgtggcccagtgtcaccgtcctcagatatcatcacaacatccttgtttctagaagctgcacacagccctgatgccagcagcgagcccacctttactgtagagagcattgccgttactaggagatcccatgcacaaagtgcagcattcccagggaaggcctcagagaatgctcctctctccagcattctctgcctactcccttaaccaccgaaggcagggcagcaggctagtggagcagagctgcgtggtcaagtggcagggagcttaagaatcgtccaagggcggagaccagtaagtctcattaggtgatggggccagcaggtaaaagccattcatgcttatgtccagctgggcgtgtgttctcttcctgttttatcatcccttgcgctgaccatcaggtacatccctgagggcatgcaatgttcatgcgggattgactactacacactcaagcctgaggtcaacaacgaatcctttgtcatctacatgttcgtggtccacttcaccattcctatgatcgtcatcttcttctgctatgggcagctggtcttcacagtcaaggaggtatgagcaggggggccgccccagcctcgtgccgggtgggtgggcagagccaggtgggcagagctgggtgccagggttcgtacagacgccatgggctagacacaggtctgtgtccctgcaggcggctgcccagcagcaggagtcagccaccactcagaaggcagagaaggaagtcacccgcatggttatcatcatggtcatcttcttcctgatctgctggcttccctacgccagtgtggccttctacatcttcacccaccagggctccaacttcggccccatcttcatgactctgccagctttctttgctaagagctcttccatctataacccggtcatctacatcatgttgaacaagcaggtgcctgggctgaggcagggcgtgtggacgcttccaagacggagaccagcc(/t)aggtggctccagcctaagcctggccagagactgtggctgaaagtaggagtctcctgtccccacacaccccagccacagcccccaccaggagcagcacccgttgggatgaggtcatgcaggctccctcagtgttcttttctttgtttttaatgaattcatgaaagcaaaatgaggctccccactcaatggggacagcttgacaaagggcatccatccaccaagaccatcctcaacctggagtccccaattcccggggggccagcgggatctgtacccctccctcagcttgtctatcaggaacatgacaagtgtcccggcttagggctaaatgtctaggacagaatggaacacatagtagctgattaataaatgctagctggatgaagggaggaatgagtgactgactgagtggatatatgagtgaagggattaatggaagggaacatggatgtcctcaggtgcccaacctggcagatccagtcatgtctggctggaatctataagcagttttacatacctgccctggttttctctgcccccacccccaccccagttggatctcccaaatccagggccctgatagaatatggctgcttcaaagacagagagatgaggggagggaggggggagggagagagggagggagggagacacagagagggaatatgtgtgatgcgtgtgtatgtgtgtatgtgtgtgtgtaaacactttgtatataaagagtacagctggtagttatgttacaagtaacaccgactaatataattaattaaccatcctaatggtctctgcttgttagtgactgcttgggaattaggcagggcccaagcactcagataaggtatttccctcagcctcagtaggcttttgcaaatgacccaggccttcaggcctgtgcagggctagagctggattacagagataaatgacagtgacagcaacgtgagctgcagcccttaggactgagaaagcatcgagaccaggggtctccggcaaggcctaggtcctcccttcagtatggaaaccttgcctcatgtctctcagcctccttggcctgtggagatccagcccttcctcttggcttctggatacatttgctcttctacaccagcaaccaagtggcaacagttccaggccagtatggagttttagaagccatgccaatatgcccaccttcagggagcagctgagtccttgatgccacccttgttctgaagagttcagaaacacagtgcaagacatgaccaggcctcatccttaggatgctcatggatccagttcttagctcccttgttggatatgctgttttccttggcctttggtcttttctttatcccagagggttttggctttaaggccaacaggaactatggggtaccagaattgagcagcctcagtctgcatccctcctctatagaaccacagctgggccctcagcaggcccaactctgcatggggacagaggcattaaaagctcagctcctacacttggtggcagtggtggtctgttgctctcaagctctttcaaaatggatggaaactgggacgcttccctgacccctggttatgaaagactagactgtgtggggacaaacagtccagagtcccggggaatgtgatagagcagctccatcatttttagaaacccaatttgaggcagtatagagagatggtgacctctataagcctctgtatctgcaaagaggagcttagacctgcccttgaggggattatatgagatttaagggacttatgtggccagcctacttcctggcatgctgaagacattggcacactctggtattctagaccttggctcagagctgcctttactaggatactgtcacttagcaaaagaatgggatggagcctcagatgtggagtgacaccatcttccaagaaggaaagggtgccagggtctgggatgaaagccctttggtgctatgttgggcaagggcgagtgccagcaaggggttatttgcttgctctctccatcagtgatgaggttccatttggtcacaagaaattcaccccaattgctgaaacagaggctgactattggcttataggcatgaaaccccactc NG 10 8 17 8 24 0 100 5 10
    250304004512_392m aagagcttctgttttggcacacgggttcttcaccccatccctttctcctcgccagcccaaactcactgcagtcgctaaggcttggatcaagcctcaaaccagaagcttgcatcctagcctgctctctctgaggtgaggttagagctggaggactgacggctactaactgccttacaggtgaaatcgccctgtggtccctggtggtcctggccattgagcgctacgtggtggtctgcaagccgatgagcaacttccgcttcggggagaatcacgctatcatgggtgtggtcttcacctggatcatggcgttggcctgtgctgctcccccactcgttggctggtccaggtaatggcactgagtatcgggtctggcaaggtctttgtgggattccctttgaggacacagagccctcggattggttccaggcataatgtaacatggtattgccccccgaaaaccatcctggtgactttcccaggctaaggtctaaggtaggggagaagagagggactgaatggtccaatcagtcttattccatgtctgagacccataacaaggagaaccctggacattccaacccttcaccttggccgagtccctaatcctcggctaagccaaggccaaaccacaatcctctttggttgagttctggccgtgggcctctctctctcttcctctctctctctcactcaccttggaccttagcccctggagaggctgaaccttcccaaaatgcatggtgacattgtagccccaggaactgggtcccatccagcctccaggccaccatatctaaatgagacaagagaaggttgggacagtggtttggacacctagacaggctatgaggtacacagagcctcagagactctccattctctgtcctcatgtcctccccaccccgggagcccacccagtggccctcactgagtcagagcccctcactcctcactggcctctttcctcatcctcacccacctggcttggcaggcctctacagacacactcagtggacacttgggtttctgagtgtggcccagtgtcaccgtcctcagatatcatcacaacatccttgtttctagaagctgcacacagccctgatgccagcagcgagcccacctttactgtagagagcattgccgttactaggagatcccatgcacaaagtgcagcattcccagggaaggcctcagagaatgctcctctctccagcattctctgcctactcccttaaccaccgaaggcagggcagcaggctagtggagcagagctgcgtggtcaagtggcagggagcttaagaatcgtccaagggcggagaccagtaagtctcattaggtgatggggccagcaggtaaaagccattcatgcttatgtccagctgggcgtgtgttctcttcctgttttatcatcccttgcgctgaccatcaggtacatccctgagggcatgcaatgttcatgcgggattgactactacacactcaagcctgaggtcaacaacgaatcctttgtcatctacatgttcgtggtccacttcaccattcctatgatcgtcatcttcttctgctatgggcagctggtcttcacagtcaaggaggtatgagcaggggggccgccccagcctcgtgccgggtgggtgggcagagccaggtgggcagagctgggtgccagggttcgtacagacgccatgggctagacacaggtctgtgtccctgcaggcggctgcccagcagcaggagtcagccaccactcagaaggcagagaaggaagtcacccgcatggttatcatcatggtcatcttcttcctgatctgctggcttccctacgccagtgtggccttctacatcttcacccaccagggctccaacttcggccccatcttcatgactctgccagctttctttgctaagagctcttccatctataacccggtcatctacatcatgttgaacaagcaggtgcctgggctgaggcagggcgtgtggacgcttccaagacggagaccagcc(/t)aggtggctccagcctaagcctggccagagactgtggctgaaagtaggagtctcctgtccccacacaccccagccacagcccccaccaggagcagcacccgttgggatgaggtcatgcaggctccctcagtgttcttttctttgtttttaatgaattcatgaaagcaaaatgaggctccccactcaatggggacagcttgacaaagggcatccatccaccaagaccatcctcaacctggagtccccaattcccggggggccagcgggatctgtacccctccctcagcttgtctatcaggaacatgacaagtgtcccggcttagggctaaatgtctaggacagaatggaacacatagtagctgattaataaatgctagctggatgaagggaggaatgagtgactgactgagtggatatatgagtgaagggattaatggaagggaacatggatgtcctcaggtgcccaacctggcagatccagtcatgtctggctggaatctataagcagttttacatacctgccctggttttctctgcccccacccccaccccagttggatctcccaaatccagggccctgatagaatatggctgcttcaaagacagagagatgaggggagggaggggggagggagagagggagggagggagacacagagagggaatatgtgtgatgcgtgtgtatgtgtgtatgtgtgtgtgtaaacactttgtatataaagagtacagctggtagttatgttacaagtaacaccgactaatataattaattaaccatcctaatggtctctgcttgttagtgactgcttgggaattaggcagggcccaagcactcagataaggtatttccctcagcctcagtaggcttttgcaaatgacccaggccttcaggcctgtgcagggctagagctggattacagagataaatgacagtgacagcaacgtgagctgcagcccttaggactgagaaagcatcgagaccaggggtctccggcaaggcctaggtcctcccttcagtatggaaaccttgcctcatgtctctcagcctccttggcctgtggagatccagcccttcctcttggcttctggatacatttgctcttctacaccagcaaccaagtggcaacagttccaggccagtatggagttttagaagccatgccaatatgcccaccttcagggagcagctgagtccttgatgccacccttgttctgaagagttcagaaacacagtgcaagacatgaccaggcctcatccttaggatgctcatggatccagttcttagctcccttgttggatatgctgttttccttggcctttggtcttttctttatcccagagggttttggctttaaggccaacaggaactatggggtaccagaattgagcagcctcagtctgcatccctcctctatagaaccacagctgggccctcagcaggcccaactctgcatggggacagaggcattaaaagctcagctcctacacttggtggcagtggtggtctgttgctctcaagctctttcaaaatggatggaaactgggacgcttccctgacccctggttatgaaagactagactgtgtggggacaaacagtccagagtcccggggaatgtgatagagcagctccatcatttttagaaacccaatttgaggcagtatagagagatggtgacctctataagcctctgtatctgcaaagaggagcttagacctgcccttgaggggattatatgagatttaagggacttatgtggccagcctacttcctggcatgctgaagacattggcacactctggtattctagaccttggctcagagctgcctttactaggatactgtcacttagcaaaagaatgggatggagcctcagatgtggagtgacaccatcttccaagaaggaaagggtgccagggtctgggatgaaagccctttggtgctatgttgggcaagggcgagtgccagcaaggggttatttgcttgctctctccatcagtgatgaggttccatttggtcacaagaaattcaccccaattgctgaaacagaggctgactattggcttataggcatgaaaccccactc NG 10 8 17 8 24 0 100 5 10
    250304004327_dobp aagagcttctgttttggcacacgggttcttcaccccatccctttctcctcgccagcccaaactcactgcagtcgctaaggcttggatcaagcctcaaaccagaagcttgcatcctagcctgctctctctgaggtgaggttagagctggaggactgacggctactaactgccttacaggtgaaatcgccctgtggtccctggtggtcctggccattgagcgctacgtggtggtctgcaagccgatgagcaacttccgcttcggggagaatcacgctatcatgggtgtggtcttcacctggatcatggcgttggcctgtgctgctcccccactcgttggctggtccaggtaatggcactgagtatcgggtctggcaaggtctttgtgggattccctttgaggacacagagccctcggattggttccaggcataatgtaacatggtattgccccccgaaaaccatcctggtgactttcccaggctaaggtctaaggtaggggagaagagagggactgaatggtccaatcagtcttattccatgtctgagacccataacaaggagaaccctggacattccaacccttcaccttggccgagtccctaatcctcggctaagccaaggccaaaccacaatcctctttggttgagttctggccgtgggcctctctctctcttcctctctctctctcactcaccttggaccttagcccctggagaggctgaaccttcccaaaatgcatggtgacattgtagccccaggaactgggtcccatccagcctccaggccaccatatctaaatgagacaagagaaggttgggacagtggtttggacacctagacaggctatgaggtacacagagcctcagagactctccattctctgtcctcatgtcctccccaccccgggagcccacccagtggccctcactgagtcagagcccctcactcctcactggcctctttcctcatcctcacccacctggcttggcaggcctctacagacacactcagtggacacttgggtttctgagtgtggcccagtgtcaccgtcctcagatatcatcacaacatccttgtttctagaagctgcacacagccctgatgccagcagcgagcccacctttactgtagagagcattgccgttactaggagatcccatgcacaaagtgcagcattcccagggaaggcctcagagaatgctcctctctccagcattctctgcctactcccttaaccaccgaaggcagggcagcaggctagtggagcagagctgcgtggtcaagtggcagggagcttaagaatcgtccaagggcggagaccagtaagtctcattaggtgatggggccagcaggtaaaagccattcatgcttatgtccagctgggcgtgtgttctcttcctgttttatcatcccttgcgctgaccatcaggtacatccctgagggcatgcaatgttcatgcgggattgactactacacactcaagcctgaggtcaacaacgaatcctttgtcatctacatgttcgtggtccacttcaccattcctatgatcgtcatcttcttctgctatgggcagctggtcttcacagtcaaggaggtatgagcaggggggccgccccagcctcgtgccgggtgggtgggcagagccaggtgggcagagctgggtgccagggttcgtacagacgccatgggctagacacaggtctgtgtccctgcaggcggctgcccagcagcaggagtcagccaccactcagaaggcagagaaggaagtcacccgcatggttatcatcatggtcatcttcttcctgatctgctggcttccctacgccagtgtggccttctacatcttcacccaccagggctccaacttcggccccatcttcatgactctgccagctttctttgctaagagctcttccatctataacccggtcatctacatcatgttgaacaagcaggtgcctgggctgaggcagggcgtgtggacgcttccaagacggagaccagcc(/t)aggtggctccagcctaagcctggccagagactgtggctgaaagtaggagtctcctgtccccacacaccccagccacagcccccaccaggagcagcacccgttgggatgaggtcatgcaggctccctcagtgttcttttctttgtttttaatgaattcatgaaagcaaaatgaggctccccactcaatggggacagcttgacaaagggcatccatccaccaagaccatcctcaacctggagtccccaattcccggggggccagcgggatctgtacccctccctcagcttgtctatcaggaacatgacaagtgtcccggcttagggctaaatgtctaggacagaatggaacacatagtagctgattaataaatgctagctggatgaagggaggaatgagtgactgactgagtggatatatgagtgaagggattaatggaagggaacatggatgtcctcaggtgcccaacctggcagatccagtcatgtctggctggaatctataagcagttttacatacctgccctggttttctctgcccccacccccaccccagttggatctcccaaatccagggccctgatagaatatggctgcttcaaagacagagagatgaggggagggaggggggagggagagagggagggagggagacacagagagggaatatgtgtgatgcgtgtgtatgtgtgtatgtgtgtgtgtaaacactttgtatataaagagtacagctggtagttatgttacaagtaacaccgactaatataattaattaaccatcctaatggtctctgcttgttagtgactgcttgggaattaggcagggcccaagcactcagataaggtatttccctcagcctcagtaggcttttgcaaatgacccaggccttcaggcctgtgcagggctagagctggattacagagataaatgacagtgacagcaacgtgagctgcagcccttaggactgagaaagcatcgagaccaggggtctccggcaaggcctaggtcctcccttcagtatggaaaccttgcctcatgtctctcagcctccttggcctgtggagatccagcccttcctcttggcttctggatacatttgctcttctacaccagcaaccaagtggcaacagttccaggccagtatggagttttagaagccatgccaatatgcccaccttcagggagcagctgagtccttgatgccacccttgttctgaagagttcagaaacacagtgcaagacatgaccaggcctcatccttaggatgctcatggatccagttcttagctcccttgttggatatgctgttttccttggcctttggtcttttctttatcccagagggttttggctttaaggccaacaggaactatggggtaccagaattgagcagcctcagtctgcatccctcctctatagaaccacagctgggccctcagcaggcccaactctgcatggggacagaggcattaaaagctcagctcctacacttggtggcagtggtggtctgttgctctcaagctctttcaaaatggatggaaactgggacgcttccctgacccctggttatgaaagactagactgtgtggggacaaacagtccagagtcccggggaatgtgatagagcagctccatcatttttagaaacccaatttgaggcagtatagagagatggtgacctctataagcctctgtatctgcaaagaggagcttagacctgcccttgaggggattatatgagatttaagggacttatgtggccagcctacttcctggcatgctgaagacattggcacactctggtattctagaccttggctcagagctgcctttactaggatactgtcacttagcaaaagaatgggatggagcctcagatgtggagtgacaccatcttccaagaaggaaagggtgccagggtctgggatgaaagccctttggtgctatgttgggcaagggcgagtgccagcaaggggttatttgcttgctctctccatcagtgatgaggttccatttggtcacaagaaattcaccccaattgctgaaacagaggctgactattggcttataggcatgaaaccccactc NGG 10 8 17 8 24 0 100 5 10