Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    260424130943_98t7 GTGTAGTGTTCCTAGATTCAGCTGAAGACCTG(+CGACTACATAGATC)GTACCTAGGGTTCCTAACGGGATGGTGTTAG NGG 10 8 17 8 24 0 100 5 10
    260424130936_78dl GTGTAGTGTTCCTAGATTCAGCTGAAGACCTG(+CGACTACATAGATC)GTACCTAGGGTTCCTAACGGGATGGTGTTAG NGG 10 8 17 8 24 0 100 5 10
    260424130923_5d2x CAAAACCATCTGTAGCTCCTCCACTTTCAGGG(+CGACTACATAGATC)GATCCGGCACCCTCTGCAGGCACTAGGAGCA NGG 10 8 17 8 24 0 100 5 10
    260424130907_oju8 ACTTCTGCTCCTAGTGCCTGCAGAGGGTGCCG(+CGACTACATAGATC)GATCCCCTGAAAGTGGAGGAGCTACAGATGG NGG 10 8 17 8 24 0 100 5 10
    260424130853_2yep TGCATTTTCCTGACTGATGATTGATGTGGGAG(+CGACTACATAGATC)GATCCATCCCAATGTGGGCAACTATAAGAAA NGG 10 8 17 8 24 0 100 5 10
    260424130837_wu2m CCTGCTTTCTTATAGTTGCCCACATTGGGATG(+CGACTACATAGATC)GATCCTCCCACATCAATCATCAGTCAGGAAA NGG 10 8 17 8 24 0 100 5 10
    260424130824_dvv0 CTCCCCATGGATTCATGTGCACCCATCTGGAG(+CGACTACATAGATC)GTACCAGATTCCTCCATGGCAGATCTGTTGG NGG 10 8 17 8 24 0 100 5 10
    260424130809_rdek TGTTCCCAACAGATCTGCCATGGAGGAATCTG(+CGACTACATAGATC)GTACCTCCAGATGGGTGCACATGAATCCATG NGG 10 8 17 8 24 0 100 5 10
    260424130755_qn02 GACGCTTTTGTTAGAAACCTGAAGCCAGTTTG(+CGACTACATAGATC)GATCCTGGTGATCACTGTGTTATCAGTAGCA NGG 10 8 17 8 24 0 100 5 10
    260424130719_9jxm AAGTGTTGTTGGGCAGTCCCGTGTCATCCATG(+CGACTACATAGATC)GTACCTGCTTCAATTTCACACATAGCTGGTA NGG 10 8 17 8 24 0 100 5 10