Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250401195108_ttuf CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250330232607_f27k CCATCAGCAGTGATTACATGATCTCCACAGCAACAAGAGCAGAGATGGGTGAAAATGGTGAGAACCCCATGTTCTCTGACATCAACACCTACGACCTGTTTGTCTGGATGCACTACTATGTGTCCCGGGACGCCTTCTTAGGAGGGCCAGGGAAC(+TGA)GTATGGAGGGACATCGACTTTGCCCATGAATCTGCAGCATTTCTGCCATGGCACCGAGTCTACCTGCTTCACTGGGAAAATGAGATAAGGAAGCTGACGGGAGATTTCAACTTCACTATCCCATACTGG NGG 10 8 17 8 24 0 100 5 10
    250330231156_iut1 CCATCAGCAGTGATTACATGATCTCCACAGCAACAAGAGCAGAGATGGGTGAAAATGGTGAGAACCCCATGTTCTCTGACATCAACACCTACGACCTGTTTGTCTGGATGCACTACTATGTGTCCCGGGACGCCTTCTTAGGAGGGCCAGGGAAC(-GT)ATGGAGGGACATCGACTTTGCCCATGAATCTGCAGCATTTCTGCCATGGCACCGAGTCTACCTGCTTCACTGGGAAAATGAGATAAGGAAGCTGACGGGAGATTTCAACTTCACTATCCCATACTGG NGG 10 8 17 8 24 0 100 5 10
    250330231117_lmpo CCATCAGCAGTGATTACATGATCTCCACAGCAACAAGAGCAGAGATGGGTGAAAATGGTGAGAACCCCATGTTCTCTGACATCAACACCTACGACCTGTTTGTCTGGATGCACTACTATGTGTCCCGGGACGCCTTCTTAGGAGGGCCAGGGAAC(-ATG)GAGGGACATCGACTTTGCCCATGAATCTGCAGCATTTCTGCCATGGCACCGAGTCTACCTGCTTCACTGGGAAAATGAGATAAGGAAGCTGACGGGAGATTTCAACTTCACTATCCCATACTGG NGG 10 8 17 8 24 0 100 5 10
    250330231107_wtif CCATCAGCAGTGATTACATGATCTCCACAGCAACAAGAGCAGAGATGGGTGAAAATGGTGAGAACCCCATGTTCTCTGACATCAACACCTACGACCTGTTTGTCTGGATGCACTACTATGTGTCCCGGGACGCCTTCTTAGGAGGGCCAGGGAAC(-GTATG)GAGGGACATCGACTTTGCCCATGAATCTGCAGCATTTCTGCCATGGCACCGAGTCTACCTGCTTCACTGGGAAAATGAGATAAGGAAGCTGACGGGAGATTTCAACTTCACTATCCCATACTGG NGG 10 8 17 8 24 0 100 5 10
    250330230944_ptft CCATCAGCAGTGATTACATGATCTCCACAGCAACAAGAGCAGAGATGGGTGAAAATGGTGAGAACCCCATGTTCTCTGACATCAACACCTACGACCTGTTTGTCTGGATGCACTACTATGTGTCCCGGGACGCCTTCTTAGGAGGGCCAGGGAAC(+TGA)GTATGGAGGGACATCGACTTTGCCCATGAATCTGCAGCATTTCTGCCATGGCACCGAGTCTACCTGCTTCACTGGGAAAATGAGATAAGGAAGCTGACGGGAGATTTCAACTTCACTATCCCATACTGG NGG 10 8 17 8 24 0 100 5 10
    250330225030_1hh3 GTGCGCAGGAATATCCTGACCATGTCAGCTGCGGAGCAACAGAAGTTTATCTCTTATCTGAACCTGGCCAAGAACACCATCAGCAGTGATTACATGATCTCCACAGCAACAAGAGCAGAGATGGGTGAAAATGGTGAGAACCCCATGTTCTCTGACATCAACACCTACGACCTGTTTGTCTG(+A)GATGCACTACTATGTGTCCCGGGACGCCTTCTTAGGAGGGCCAGGGAACGTATGGAGGGACATCGACTTTGCCCATGAATCTGCAGCATTTCTGCCATGGCACCGAGTCTACCTGCTTCACTGGGAAAATGAGATAAGGAAGCTGACGGGAGATTTCAACTTCACTATCCCATACTGG NGG 10 8 17 8 24 0 100 5 10
    250330225022_pfrj GTGCGCAGGAATATCCTGACCATGTCAGCTGCGGAGCAACAGAAGTTTATCTCTTATCTGAACCTGGCCAAGAACACCATCAGCAGTGATTACATGATCTCCACAGCAACAAGAGCAGAGATGGGTGAAAATGGTGAGAACCCCATGTTCTCTGACATCAACACCTACGACCTGTTTGTCTG(-G)ATGCACTACTATGTGTCCCGGGACGCCTTCTTAGGAGGGCCAGGGAACGTATGGAGGGACATCGACTTTGCCCATGAATCTGCAGCATTTCTGCCATGGCACCGAGTCTACCTGCTTCACTGGGAAAATGAGATAAGGAAGCTGACGGGAGATTTCAACTTCACTATCCCATACTGG NGG 10 8 17 8 24 0 100 5 10
    250330141753_142y CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250328053605_lzdq GATTGAGCATACTAAAAGTGACTCTCTAATTTTCTATTTTTGGTAATAGGACATCTCCAAGTTTGCAGAGAAAGACAATATAGTTCTTT(/G)GAGAAGGTGGAATCACACTGAGTGGAGGTCAACGAGCAAGAATTTCTTTAGCAAGGTGAATAACTAATTATTGGTCTAGCAAGCATTTGCTGTAAATGTCATTCATGT NG 10 8 17 8 24 0 100 5 10