Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    260424132420_b7pn AGATGGCGAGCCTCCCTGTTGTGGGTAGTCAG(+CGACTACATAGACG)GTACCTGCAGACAGAGGATACCTTGGTCCTA NGG 6 8 17 8 24 0 100 5 10
    260424132405_tna2 TGCAGAGATGGTTTATGAGGCGGGTCCGAAGG(+CGACTACATAGACG)GTACCCTTCACCTTGCCTGGAGATATACAAA NGG 6 8 17 8 24 0 100 5 10
    260424132352_8xmz TCTGTGCTCAGGTCACACAGGGGGCTTCAGAG(+CGACTACATAGACG)GATCCTTATGAAAGATGAGTTAAAAAATGTC NGG 6 8 17 8 24 0 100 5 10
    260424132338_uyys GAAGAGAGACAAGGAGAAGCAGAAAGCAGAAG(+CGACTACATAGACG)GTACCCTTTTATTGGCATTTTAACAATGGCA NGG 6 8 17 8 24 0 100 5 10
    260424132322_uboo AGAATCGCTTAAGCAGGGCTGTGGGCCTGCAG(+CGACTACATAGACG)GATCCCCAGGTCCCTTTGGACCGGAGGGGGA NGG 6 8 17 8 24 0 100 5 10
    260424132304_egxe GACTTTATGTGGGAGCTGGGGATTTGAACTAG(+CGACTACATAGACG)GATCCTTGTATAGCAAGCATCCATATTCTGA NGG 6 8 17 8 24 0 100 5 10
    260424132248_v624 ATGGTTCAGAATATGGATGCTTGCTATACAAG(+CGACTACATAGACG)GATCCTAGTTCAAATCCCCAGCTCCCACATA NGG 6 8 17 8 24 0 100 5 10
    260424132232_hoh8 GACCCACAGGAACTATGAAGGAAGAAGCGTAG(+CGACTACATAGACG)GATCCCAGAGCAATCATATAACCGAACTCTG NGG 6 8 17 8 24 0 100 5 10
    260424132218_cc9b GTAGCCTTTGCGATTGTGTGATCCAAGAGCTG(+CGACTACATAGACG)GATCCATACAATTTGTGCCACCCTGAAATTG NGG 6 8 17 8 24 0 100 5 10
    260424132159_04b3 AACTGGGGTACACTTTCCAGAACTGCCTTGAG(+CGACTACATAGACG)GTACCTAAAACTTCCACCGAATGAACCTTGG NGG 6 8 17 8 24 0 100 5 10