Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250908090318_jkfaqfso CAGTTTCCCTATATTATTTGGAGTCAATTCTTACGTAATTTGTTGTTTATTTCTTCAGTGAACATTGTCTTTTTCTGGCAGCTGAAACTGCACACAACTGA(/G)TACACATATTTAATTTGTATTCCTTTGTAGTAATACATTTTAACCATGATTTATGTTTGTGAATTTACTGGATGTTTTCTTTCTGAATTAAAGATTGTCAA NG 10 8 17 8 24 0 100 5 10
    250908090318_jkfapomz TTGTTTTGTTATTTTTAAGTTACCTACAGATAATGCATTTCATTATTGCCTACTTCCAACTCTCCCTATGTAGTATGTTTCTGATCTTCTGTGCTTGTGCA(/G)GTGTAGATGAGCTCACCGCTTTAATTTTTAAGATAGAAATGTGGAAAACTCTTGAATCATAGACTAGGGGAAGGAACCAGAGGTCCTCTAAAATATCAGAG NG 10 8 17 8 24 0 100 5 10
    250908090318_jkfapne7 GGCTGGTTTCTTTCAAGTTTAAAAAAAGCCCCCTTCTCCATGCACAAGTTCATTCCCTCCATGCTGTCGTCATCGACATATACAGGTGTGTACGTAGGTGT(/C)ATGTGTGTACTCTGCACGTATATCTCTTCTGTCTTGAAGACCCTGTTAACTTTGTGAAATAACTTCCACATTTCAACACACTTCACAGTACAGGAAATAAA NG 10 8 17 8 24 0 100 5 10
    250908090318_jkfapazu CATTTCAAGAGGAAACTTGATAATTTTAAACTCTTTTCCCCCTTTACCGTCATCTATCCTCTATCTGTATCAAGCTCACTGCGGATCCTGCTGTAAAACTG(/C)GTGGGTAGCTGGGCTGTCCGTTACCACCACAGCTACATCTTCTGTTTCTTTGAATAGAAAGCACCACAGTGTTTTGCACTTGCCTCCAGTAAAGTGCCCTG NG 10 8 17 8 24 0 100 5 10
    250908090318_jkfaoz2e TGGCCACTGTGCCTGGCCCGGGATATTTTCTTATTATCTAAACTTTAAAAGAAATTATTAAAATTATTTAAAGCAAGAATAGTAAAAATGTAGCGTGGAAC(/T)CTTTAACAAATGTAGAAGTAAAAATATAACATAAGTAGAAGTAAAAAATAATTGGATTGTTTAGACTATTTACATATAATGTGATTATTGATATAGTTAAG NG 10 8 17 8 24 0 100 5 10
    250908090318_jkfaousi ACTGGCTATGGTACTTTGGGGAGATAGGAAGGACAGGACGACAAGAGACAGGACACCAGTGAGTGGCTGGGCCAGGCGGCCCCATTTCCTAAAACCAAGAT(/C)GGCTGCCGCTGCTGCCCAAGCCCTCAAAGGCAGACAGTCCTCATGCGCAGCACCAGCACACACACAGGTAACAAGACCAGGGGAGGGGACCCTAAGTGTCC NG 10 8 17 8 24 0 100 5 10
    250908090318_jkfaokin GTAATAACTGCAACCAATCAGTGTTATTCAGTGCTATGCCTCCTTGTAATGGGTAGTTATTAATTATTTTCAGAGCTTTCCGGAAATACTGTCCTAACTGG(/T)CTATGTTTAGGATCTTTGTTATCTCTGAAGACAAAGAAAGAAGCTAGGACTCTTAATTTTGGGGTGCTTCTTGACTCTTAGTTGGGAAACTGAAAATATTT NG 10 8 17 8 24 0 100 5 10
    250908090318_jkfaoa99 TCAAAGAAAGGAAAGGCTACTAACACATTTATCAGGTAATGTATGTCACAGTCCGTGTTCCACCCAGCCTTGTTATCCCCACATTCTTTCAAGATAGGAGT(/C)CATCTTGGTTTTTCGGGTGAAAAACGGAAGGTCAGAGAGGAAGGGGCTTGTCCAAGGTCTCATGTCTGGTTAACAGAATTTCAAACTAGATGTTTGATTCT NG 10 8 17 8 24 0 100 5 10
    250908090318_jkfan9r4 TACATATATATACACATAGGTATAGTCTTGTCTCTACATGTATATATGTTAATGCTAGAAGGAACAGATACATTTTTCCTTTTAAAATTTCACGCAGCCTA(/G)ATATTGCAAATAGAACAGTAGTTTCCATTTGAAATGTGCTAGGTTGAAGATGTCAGTATTCTCTGATATTCAGTGAATTGACAGAATTTTATGAGAAACTA NG 10 8 17 8 24 0 100 5 10
    250908090318_jkfamq7h AGAAACTCAAATTGTTCCTGGGCAAATCACAACATGGGGAATTTTATTCATAAATGTCCACAGAAACAGTAAATGTTCTCGCTTCAGTACTTAATTCATCT(/G)AATCCCTCCTGTTTGTCTCAAATTATAGGATAACTTTGAAACTTTCTGAATTAACGTTATTTAAAAGGAAATGTAGATGTTATTTTAGTCTCTATCTTCAT NG 10 8 17 8 24 0 100 5 10