Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250908090534_8s85cvqs TTGCACAATTCAATTCATTCTCTACATTAGTAGCGCTTAAAAAAAAAAAAAAAAAAAAGGCAAAATTAATATCATTCTGGGGGCGGGCGGGGGGCAGTGGG(/A)AAGCAACTATTTGTACAGCAGAGGTCCTAAGCTGGTTTTGCCATGAGCTGTTTGGCTTTGAGCAGTTGGTTCTGCAGCTGGTGCAGCAGAGTAAGGACAGG NG 10 8 17 8 24 0 100 5 10
    250908090534_8s85cpsq GTCAACAAACTGTTCGTTTTTAAGTTACAAATTTGAATTTAATGTTGTCATCATCGTCATGTGTTTCCCCAAAGGGAAGCCAGTCATTGACCATTTAAAAA(/C)GTCTCCTGCTAAGTATGGAAATCAGACAGTAAGAGAAAGCCAAAAAGCAATGCAGAGAAAGGTGTCCAAGCTGTCTTCAGCCTTCCCCAGCTAAAGAGCAG NG 10 8 17 8 24 0 100 5 10
    250908090534_8s85cgw4 AGGTCGGGGGAGGGGAGCCCCCCTCGGGCTGTGGTTAGAGCGGGAGAGGAACTTCCCAGACTAGCTGGCACAGAGCCTCGGGAAGGCGGCGGGCACTGCAG(/A)GTGGTTTACGGGAAGTGCTGCAGCCTTGGGGTGGGGACAGCGTGGCCAGACCCACCGCCTCATCTGCACACCTGGGCTCAAGCGCTAATGACGACAGGGGA NG 10 8 17 8 24 0 100 5 10
    250908090534_8s85c9lq CATCAGTTTTGGAAAATTCTTAGCCATTTTTTTTTTTTTTACTATTTTTTCCCCTGTTCTCCCTCTTCTCCCTGAGACTTCAGTTTTGTGTGTGTGTGCAC(/T)ATGTGTGTATGCATTCGTGCGTACGAGTGTGTGCATGTGTGCGCCCGTGTGTATGTGCATATGTGAGTGTGTGTGGCTGTAACCTGCACGTCTCTTCTGTC NG 10 8 17 8 24 0 100 5 10
    250908090534_8s85c8zt TAAATATTTATCTGTCAGTTTTGACAGATTGGGGCCAGCTTGATGTTTTAAATCTTCAGCCCGGTATGAAAACTTAAAGGTATATATTCAATTTTTTACCA(/G)TTTTATGGAAAATATTTAAAATCTGTTTTTACAGGGTTTTTTTTTTTTTTTTTTTTTTGTAATCTGTGCCATGAAATTTGAAAACCACCAAAAATCAAGGG NG 10 8 17 8 24 0 100 5 10
    250908090534_8s85bdhz AAGTAAAGAGCTATAATCCCAACCTAAACTTTTCTGAGATTTTACAATAATTAGATCTTTTTCCAAGTTAATTGGGTTTTCCCTTCTCCCAGTCATAGGTG(/A)GTTTTTATCATCAAGACAGACTGATATTTTGTCAGGATATTTTCTTTTACAGTGTTTGATGTGCATAATGCCAGAGTTATTTTTTTATTATTCATTTTCTC NG 10 8 17 8 24 0 100 5 10
    250908090534_8s85ayny AGTTACTCAAATAATGTATGTGTACCTTATCTTTACTTCAGGGTCTCATGGTTGTGGTCTTGGGTCAAACCACAGTAGAACTTCACAACACAAAAACTGAT(/C)CTGATGCCAGAATTAATGGTGCAGGGAAAAACCAAATCATAGTATGGATTACTTTAAATACATAAAAGATGAATCTGTACACTACGTGACAGTGGTGTTAA NG 10 8 17 8 24 0 100 5 10
    250908090534_8s85algu ACAACTAAACTCAACCCATTATGAGGCAATCTACACTGTCCATACCTATGCTTAGACAGTAGGTGTAAGATTGATAAAAGTAACATTGTTTATTACAAGAC(/T)TGAGAAATTGCTTTTAGCTCTGGGCGAGATGCCCTGAGATTCCAATGCCAATGCTAGATGAGTGATACTGGTATGCCTGCCTTAGCAGTGGGTAATTTGAA NG 10 8 17 8 24 0 100 5 10
    250908090534_8s859prx TCAAAGAAAGGAAAGGCTACTAACACATTTATCAGGTAATGTATGTCACAGTCCGTGTTCCACCCAGCCTTGTTATCCCCACATTCTTTCAAGATAGGAGT(/C)CATCTTGGTTTTTCGGGTGAAAAACGGAAGGTCAGAGAGGAAGGGGCTTGTCCAAGGTCTCATGTCTGGTTAACAGAATTTCAAACTAGATGTTTGATTCT NG 10 8 17 8 24 0 100 5 10
    250908090534_8s859098 GTGAGCTGGGCAGAGCTGGGCTGCCAGAAGCCTTTTTCACTCTGCAGTGTGATTGGACTGCCAGCCAGGTTAATAAACTGAAAAATGTGATTGGCTTCCTC(/G)TCGCCGTGTTGTGAGGGCAAAGGAGAGAAGGGAGAAAAAAAAAAAAAAACCACACACACCCATACACACATACCAGAAAAAGAAAGAAAGGATGGAGACGG NG 10 8 17 8 24 0 100 5 10