Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250408193810_jazj TGTCCCGGGACGCTCTGCTCGGTGGGCCTGGGAATGTGTGGGCTGATATTGATTTTGCGCATGAGTCGGCGGCGTTTCTGCCTTGGCATCGGGTGTATCTGCTGTTTTGGGAGCATGAGATCCGGAAGCTGACGGGAGACTTTAACTTCACCATCCCGTACTGGGACTGGCGGGACGCGCAGGACT(+A)GTCAGGTGTGCACGGATGAGCTGATGGGGGCGCGCAGCAGCCTCAACCGCAGCCTGATCAGCCCCTCCTCTGTGTTCTCATCCTGGAAGGTAAAGCAGACTTTTATTTGACTTTTTTTAAAACAAGAATGTTAGTTCTAGGGGCGGTATGGTGGCTCAGTGGTTATCCCTATCGCTTCACAGCAAGAAGGTCACTGGTTCAAGTCCAAGTTAAATTGGTTTGAGTTGAATTGGCTGTAGTGTATGAGTGTTTGTATGAGTGTGT NGG 10 8 17 8 24 0 100 5 10
    250408193729_q8vm TGTCCCGGGACGCTCTGCTCGGTGGGCCTGGGAATGTGTGGGCTGATATTGATTTTGCGCATGAGTCGGCGGCGTTTCTGCCTTGGCATCGGGTGTATCTGCTGTTTTGGGAGCATGAGATCCGGAAGCTGACGGGAGACTTTAACTTCACCATCCCGTACTGGGACTGGCGGGACGCGCAGGACTG(+A)TCAGGTGTGCACGGATGAGCTGATGGGGGCGCGCAGCAGCCTCAACCGCAGCCTGATCAGCCCCTCCTCTGTGTTCTCATCCTGGAAGGTAAAGCAGACTTTTATTTGACTTTTTTTAAAACAAGAATGTTAGTTCTAGGGGCGGTATGGTGGCTCAGTGGTTATCCCTATCGCTTCACAGCAAGAAGGTCACTGGTTCAAGTCCAAGTTAAATTGGTTTGAGTTGAATTGGCTGTAGTGTATGAGTGTTTGTATGAGTGTGT NGG 10 8 17 8 24 0 100 5 10
    250408185654_4iod TACCCTAGTGAAGCCTTTACATGTCACTGTAAGCTGAACACTAGTGTCTTGAAGAATATCTAGTCTAATATTATGTGCTGTCATCATGATGAAGAGGAAATAAATCAGTTATTAGAGCGAACAGTGCCACTGAAGTCTCTTTCTCCCTCCACAGAAGCACTCTGCTGCTGCTGACATCACTTCCTCTCTGTCGGAGGACCAGGTTCCCGAGGCGTTCCTGGTGATGGTTCTGATCCAGTTCGGGACGATGGTGGTGGACCGAGCGCTGT(/C)ACCTGAGGAAGACTGTGATGGGGAAGGTCATCTTCCAGGTCATCCTGGTGTTCGGCATTCACTTCTGGATGTTCTTCATTCTGCCCGGAGTCACCGAGAGGTTTGAAAGCCTGTCATTGTCTTGCTTTGCTTTGTGTTTGTTTATTTTAGTTTTGTGTGGTGTTGTTTTGTTTATGGTTCGGTTGTGTTTGTTTTTTGGTTTTGTTTGTTTTTGTGTCTTGTGTCTTGTTTTGTTTTTAGTTTGGTTTAGTTAGTTTGATTTTTGTGTTGA NGG 10 8 17 8 24 0 100 5 10
    250408134707_t643 GATCCGGAAGCTGACCGGTGACTTTAACTTTACTATCCCGTACTGGGACTGGCGCGACGCGCAGGACTGTCAGGTGTGCACGGACGAGCTGATGGGGGCCCGCAGTCCTCTCAGCCCCAAC(+TGA)CTGATCAGCCCGTCCTCGGTGTTCTCCTCCTGGAAGGTAAACATGAAAACAAATTCATTTTACTCAAGAATACAGTATTTATCCCTCTAGTCATGTTGGTTCTATTCATTTGGAATTCATTCCAATTACACAAATGCTTGTTTTCTCTTGCAGACACACAAAACCAAAC NGG 10 8 17 8 24 0 100 5 10
    250408134635_zzxd AGCTTTCAGTTTCATTTGATTATCTGGATGATTTTTTTTTTTTTTTTTTTTTTCTCCCCTCTCAAAGCAATCTGCTGTGCTTCCTTGCAGGTCTAGGTGGAGCCTGTGGATATCTGATTGGAGCCATGG(-A)CTGGGGTCACTCTGCGCTTGGATTTGCACTGGGTTCCGAGTACCAAGTTATTTATTTTTTCTCATCTCTAACCTGGGGCATTTTTCTCACCATGCACCTTTTCAGCATCCCAGAGAAA NGG 10 8 17 8 24 0 100 5 10
    250408134227_wb5v GTGCTTCATCTCAGATTTCAAGAAGCATTTTAGTTTTTGCCCAGCTGAGCACATTCTGCCGAAGCTTTCAGTTTCATTTGATTATCTGGATGATTTTTTTTTTTTTTTTTTTTTTCTCCCCTCTCAAAGCAATCTGCTGTGCTTCCTTGCAGGTCTAGGTGGAGCCTGTG(/T)GATATCTGATTGGAGCCATGGACTGGGGTCACTCTGCGCTTGGATTTGCACTGGGTTCCGAGTACCAAGTTATTTATTTTTTCTCATCTCTAACCTGGGGCATTTTTCTCACCATGCACCTTTTCAGCATCCCAGAGAAA NGG 10 8 17 8 24 0 100 5 10
    250407222231_hjji TCCTCGGCCCTGCACCACACCGGACGTCCTGCAGAGCAAGCGCTGCTGTCCAGTTTGGCCGGGCGACGGTTCGGTGTGCGGATCCCTTTCGGGTCGAGGCTTCTGCCAGGATGTCACGGTCTCCGACCTTCCCAAC(-GG)GCCGCAGTTCCCTCATTCAGACGTGGACGACCGCGAACGCTGGCCTCTGGTGTTTTACAACCAAACCTGCCAGTGCGCCGGCAACTACATGGGGTTCAACTGCGGCGAGTGCAAGTTCGGTTACTTCGGCGCCAACTGCGGGGAAAGACGGGAGTCTGTTCGCAGAAACATCTTCCAGTTGTCCATGTCCGAACAGCAGCGGTTCATCTCATACCTAAATCTCGC NGG 10 8 17 8 24 0 100 5 10
    250407221648_u7v8 GATCCGGAAGCTGACCGGTGACTTTAACTTTACTATCCCGTACTGGGACTGGCGCGACGCGCAGGACTGTCAGGTGTGCACGGACGAGCTGATGGGGGCCCGCAGTCCTCTCAGCCCCAAC(+TGA)CTGATCAGCCCGTCCTCGGTGTTCTCCTCCTGGAAGGTAAACATGAAAACAAATTCATTTTACTCAAGAATACAGTATTTATCCCTCTAGTCATGTTGGTTCTATTCATTTGGAATTCATTCCAATTACACAAATGCTTGTTTTCTCTTGCAGACACACAAAACCAAAC NGG 10 8 17 8 24 0 100 5 10
    250407221534_ph8a TCCTCGGCCCTGCACCACACCGGACGTCCTGCAGAGCAAGCGCTGCTGTCCAGTTTGGCCGGGCGACGGTTCGGTGTGCGGATCCCTTTCGGGTCGAGGCTTCTGCCAGGATGTCACGGTCTCCGACCTTCCCAAC(+TGA)GGGCCGCAGTTCCCTCATTCAGACGTGGACGACCGCGAACGCTGGCCTCTGGTGTTTTACAACCAAACCTGCCAGTGCGCCGGCAACTACATGGGGTTCAACTGCGGCGAGTGCAAGTTCGGTTACTTCGGCGCCAACTGCGGGGAAAGACGGGAGTCTGTTCGCAGAAACATCTTCCAGTTGTCCATGTCCGAACAGCAGCGGTTCATCTCATACCTAAATCTCGCG NGG 10 8 17 8 24 0 100 5 10
    250407221219_ig2u TCCTCGGCCCTGCACCACACCGGACGTCCTGCAGAGCAAGCGCTGCTGTCCAGTTTGGCCGGGCGACGGTTCGGTGTGCGGATCCCTTTCGGGTCGAGGCTTCTGCCAGGATGTCACGGTCTCCGACCTTCCCAAC(+TGA)GGGCCGCAGTTCCCTCATTCAGACGTGGACGACCGCGAACGCTGGCCTCTGGTGTTTTACAACCAAACCTGCCAGTGCGCCGGCAACTACATGGGGTTCAACTGCGGCGAGTGCAAGTTCGGTTACTTCGGCGCCAACTGCGGGGAAAGACGGGAGTCTGTTCGCAGAAACATCTTCCAGTTGTCCATGTCCGAACAGCAGCGGTTCATCTCATACCTAAATCTCGCG NGG 10 8 17 8 24 0 100 5 10