Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250908090534_8s85onye TATAGTAAAATATTGAATCACTTTATAGTAGACAATACATATGACATTTAACAAATACTTGGTTTGAAAAAGTTTGGCTTTATTCAACAATTTTAGTTTGC(/T)CCACTGATTTTCAAATGTCTGTGGGATTGCTTATAACTAAGTAGAAAGTGAAGACTGATTTTTACTTAAGAATGGGATAGAGATGAGTTTTATGGTTTTTA NG 10 8 17 8 24 0 100 5 10
    250908090534_8s85ojve GCCAAGATCACACCACCGCACTCCAGCCTGGGCAACAGAGCAAGACTCCGTCTCAAAAAAAAAAAAAAAAAAAAACACAAACCAACCCCATGGAGTTGGAT(/C)AGTATTCAACCAACACCATGTGAAAGGAAGAGATTTGGTTGGGGCTAAGATAAAGCTGCCAAACAGAATAGGTCTAAGATAAAGCTGCCAAACAGAATAGG NG 10 8 17 8 24 0 100 5 10
    250908090534_8s85ntc5 GGCTGGTTTCTTTCAAGTTTAAAAAAAGCCCCCTTCTCCATGCACAAGTTCATTCCCTCCATGCTGTCGTCATCGACATATACAGGTGTGTACGTAGGTGT(/C)ATGTGTGTACTCTGCACGTATATCTCTTCTGTCTTGAAGACCCTGTTAACTTTGTGAAATAACTTCCACATTTCAACACACTTCACAGTACAGGAAATAAA NG 10 8 17 8 24 0 100 5 10
    250908090534_8s85mkpp GGATGAAGATTCTCCTGTGCTAGATGTGCAAATGCAAGCTAGTGGCTTCAAAATAGAGAATCCCACTTTCTATAGCAGATTGTGTAACAATTTTAATGCTA(/G)TTTCCCCAGGGGAAAATGAAGGTTAGGATTTAACAGTCATTTAAAAAAAAAATTTGTTTTGACGGATGATTGGATTATTCATTTAAAATGATTAGAAGGCA NG 10 8 17 8 24 0 100 5 10
    250908090534_8s85lui6 GTGGGAGGGGAGGTGGCCTGCGGAAAGGCCTGGACCTGCCCCTCACCTGGCGTCTGGCCTTGCTTCCTTGCTTTCCGGGGTCTCCTCACTGCAGCTGAGCA(/G)GGGAATGGGTAGCAGCAGCAGCTCTCGAGTGCCAGTGCGTGAACCGTTGGGGAGGTTTCCCAAAAATGCACGTTCCTCGGCCTTCTCCCCAGAGACCCGGC NG 10 8 17 8 24 0 100 5 10
    250908090534_8s85lmmy CACTTAGCTTCTCTAGTTTCTTATGATTTCCTATTTTACACTTAACAGCAAAAGGAGGCTTCCATTTAGAAGGCACTATCTCAAACATAAATAATTTCATA(/G)TTTATTTTATCACTGTAACACTAAGGAGCTATGGCATTAAAATATAAAACTATTTGGAACTTAATGTAAACCTTTGCACATGCAGACTATATAAAGGGAAT NG 10 8 17 8 24 0 100 5 10
    250908090534_8s85kez8 TGTGGCTAGGGACATTGGAATTCTCTACCATTTTTACTGAACAAAAAATAATTTTTTTTCCTGTTTCAAAAAAGGGAATAGTGACTTTGTTTTCTCCAGGC(/T)TCCCCTTCCCACACAACCAATGTAAAATACAGCAGCCTGTATCTTAATAACCCAGCCCTACCCCCTCCCTTACCCAGCATTGTCTACTGGAGAATTTAAAT NG 10 8 17 8 24 0 100 5 10
    250908090534_8s85kdrp GGCCTCGTCCTAACATGTCCCAAAGGGGGAGAACAGTGGGTGGTGATTTGAATGAGGAACTATTGCTTTCAGTACCAAAATAAGCAGATAGAGATAGAGAC(/G)CGATTCATCTTGGCAACATGTGTTTATTTGTTGGTCAGTGCAGGTTCTGATAGGTCTATTACTTCTCTATAATAAGACAGTGTAAAAATAAACTATGTTAT NG 10 8 17 8 24 0 100 5 10
    250908090534_8s85j41k ACTGGCTATGGTACTTTGGGGAGATAGGAAGGACAGGACGACAAGAGACAGGACACCAGTGAGTGGCTGGGCCAGGCGGCCCCATTTCCTAAAACCAAGAT(/C)GGCTGCCGCTGCTGCCCAAGCCCTCAAAGGCAGACAGTCCTCATGCGCAGCACCAGCACACACACAGGTAACAAGACCAGGGGAGGGGACCCTAAGTGTCC NG 10 8 17 8 24 0 100 5 10
    250908090534_8s85iuf7 AAGAAGTCCACACAAGCAAGCCAAATTTTTAGATGACGAAGTCCATAAATAACTAGAGAATTTTTGTTATCTGTTGTTAAGTTGAAATGTATAATCATTTA(/C)TCACTAAATTGCACATTGCCTTTATTTATTTGTGCTCTGTTTTTGGTTTACAGTGTAATAATACCTCATTTAAAAAATAAAAACCACTACTGTTACATTTT NG 10 8 17 8 24 0 100 5 10