Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250620152917_92bt atcagtggcattcacagaagtgccatctttatttccttctaaaaagagtgcaaaaagaacaattttatcctcatccttggaagaatccattaccctacca(-a)aagtaatttggatgttaatttatgtttggataagacttgtttatccattgtcccttcacaaactatctcttcagacttgatgaattctgatttgacttcaaaa NGG 10 8 17 8 24 0 100 5 10
    250620124359_3671 TATCTGTCCGATCATAGAATTTCTTACTTATCACAGTAAGTTGTCTTTCCAGGGTTACAGTTTGTATGTGAAACCACTGTTCCAAGTTCAACTTAGAAAAGACTGTTGACACACAAATTGGA(-ATG)GTTCCAATAGCCTACTTTGCGAAACGGTTTGGATTCACAGAACTTCCGTTGTAAAAGTAAGTGTGTGTTATATTCATAAAATTAGCTCTAATTTTTGATATGTGAATATGGGTGGAAGTGTCAACGTTTTGGGGTAATATGGATCTCCCAGTGGCAAATAATTAGGGTCAGTGC NGG 10 8 17 8 24 0 100 5 10
    250619200448_1qov tgcagttattttgttgcctttaccttagaaaacaaagtttgaatcttgccttgtgtttctgaaggctcttactgcagtttttaatgagatactgttttctttttgtcaatcacccaggtgt(/c)ctatagatgctgcacatggatacagcccccgtgctattgacatgagtaatgt NGG 10 8 17 8 24 0 100 5 10
    250619200047_tnuq tgcagttattttgttgcctttaccttagaaaacaaagtttgaatcttgccttgtgtttctgaaggctcttactgcagtttttaatgagatactgttttctttttgtcaatcacccaggtgt(/c)ctatagatgctgcacatggatacagcccccgtgctattgacatgagtaatgt NGG 10 8 17 8 24 0 100 5 10
    250619191317_jgby TATCTGTCCGATCATAGAATTTCTTACTTATCACAGTAAGTTGTCTTTCCAGGGTTACAGTTTGTATGTGAAACCACTGTTCCAAGTTCAACTTAGAAAAGACTGTTGACACACAAATTGGA(-ATG)GTTCCAATAGCCTACTTTGCGAAACGGTTTGGATTCACAGAACTTCCGTTGTAAAAGTAAGTGTGTGTTATATTCATAAAATTAGCTCTAATTTTTGATATGTGAATATGGGTGGAAGTGTCAACGTTTTGGGGTAATATGGATCTCCCAGTGGCAAATAATTAGGGTCAGTGC NGG 10 8 17 8 24 0 100 5 10
    250619191316_ax30 TATCTGTCCGATCATAGAATTTCTTACTTATCACAGTAAGTTGTCTTTCCAGGGTTACAGTTTGTATGTGAAACCACTGTTCCAAGTTCAACTTAGAAAAGACTGTTGACACACAAATTGGA(-ATG)GTTCCAATAGCCTACTTTGCGAAACGGTTTGGATTCACAGAACTTCCGTTGTAAAAGTAAGTGTGTGTTATATTCATAAAATTAGCTCTAATTTTTGATATGTGAATATGGGTGGAAGTGTCAACGTTTTGGGGTAATATGGATCTCCCAGTGGCAAATAATTAGGGTCAGTGC NGG 10 8 17 8 24 0 100 5 10
    250619191256_35hp TATCTGTCCGATCATAGAATTTCTTACTTATCACAGTAAGTTGTCTTTCCAGGGTTACAGTTTGTATGTGAAACCACTGTTCCAAGTTCAACTTAGAAAAGACTGTTGACACACAAATTGGA(-ATG)GTTCCAATAGCCTACTTTGCGAAACGGTTTGGATTCACAGAACTTCCGTTGTAAAAGTAAGTGTGTGTTATATTCATAAAATTAGCTCTAATTTTTGATATGTGAATATGGGTGGAAGTGTCAACGTTTTGGGGTAATATGGATCTCCCAGTGGCAAATAATTAGGGTCAGTGCC NGG 10 8 17 8 24 0 100 5 10
    250619191247_oh6s TATCTGTCCGATCATAGAATTTCTTACTTATCACAGTAAGTTGTCTTTCCAGGGTTACAGTTTGTATGTGAAACCACTGTTCCAAGTTCAACTTAGAAAAGACTGTTGACACACAAATTGGAATGGTTCCAATAGCCTACTTTGCGAA(-ACG)GTTTGGATTCACAGAACTTCCGTTGTAAAAGTAAGTGTGTGTTATATTCATAAAATTAGCTCTAATTTTTGATATGTGAATATGGGTGGAAGTGTCAACGTTTTGGGGTAATATGGATCTCCCAGTGGCAAATAATTAGGG NGG 10 8 17 8 24 0 100 5 10
    250619191207_t9lc ACTGTTCCAAGTTCAACTTAGAAAAGACTGTTGACACACAAATTGGAATGGTTCCAATAGCCTACTTTGCGAA(-ACG)GTTTGGATTCACAGAACTTCCGTTGTAAAAGTAAGTGTGTGTTATATTCATAAAATTAGCTCTAATTTTTGATATGTGAATATGGGTGGAAGTGTCAACGTTTTGGGGTAATATGGATCTCCCAGTGGCAAATAATTAGGG NGG 10 8 17 8 24 0 100 5 10
    250619191151_8kqw ATCTGTCCGATCATAGAATTTCTTACTTATCACAGTAAGTTGTCTTTCCAGGGTTACAGTTTGTATGTGAAACCACTGTTCCAAGTTCAACTTAGAAAAGACTGTTGACACACAAATTGGAATGGTTCCAATAGCCTACTTTGCGAA(-ACG)GTTTGGATTCACAGAACTTCCGTTGTAAAAGTAAGTGTGTGTTATATTCATAAAATTAGCTCTAATTTTTGATATGTGAATATGGGTGGAAGTGTCAACGTTTTGGGGTAATATGGATCTCCCAGTGGCAAATAATTAGG NGG 10 8 17 8 24 0 100 5 10