Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250908090803_gowyfdf8 TGGAATGGGTTCCCGAGGCCCTCAGCCAGTGTGCAGTCGCTCCCCACTGTCCATATCACAGGACAGAACCAGCTTTCTGGAAAAGATCAAGTGTAGCCTTA(/G)CCATGGGAGGAACAATGAGGGCTCCAAAGCCACCACCCCAAGTAAATTATTCTTGTGCAATGCAAAACAGAAAATATCATAGTAGTAAGAGGGGATCTATA NG 10 8 17 8 24 0 100 5 10
    250908090803_gowyf5pm ACAGATACAATGTTGAATAAGGCAAAGCACCTGCCCTCAGGTAGCTTGCAGTCAGGGAGGTAAGGGTAGTGGGCAGAGAGACCTGGAAACAGATATTAGAC(/A)CTGCACTAAGCATGTGTGGTTATTGAACAGTAAAAATGCCACCACAAATTGCGATATGATGTAAGTAAAATGCGTACTGGCTATTGAAGACTTGGCACAGA NG 10 8 17 8 24 0 100 5 10
    250908090803_gowydu8t TATAGTAAAATATTGAATCACTTTATAGTAGACAATACATATGACATTTAACAAATACTTGGTTTGAAAAAGTTTGGCTTTATTCAACAATTTTAGTTTGC(/T)CCACTGATTTTCAAATGTCTGTGGGATTGCTTATAACTAAGTAGAAAGTGAAGACTGATTTTTACTTAAGAATGGGATAGAGATGAGTTTTATGGTTTTTA NG 10 8 17 8 24 0 100 5 10
    250908090803_gowycsyz AGCCCTCCTTCGGGAGAACTGTAAGTAAGAGGTGGGTGTGTCTAAAGACAATACCATTAATGAATGTTCTGGCCTTACCTAAAAAGGTTTAGCAATTTGGG(/A)GATAACTCTTGGATCTAGCTTATGTGCGTTCACATGCACATTTGCTAGCCCAGAGCTTTTAAAATGAGGTCTGGCATATACTTGATTACAAATGAAAACTC NG 10 8 17 8 24 0 100 5 10
    250908090803_gowycl9q AGGAGCTAACTGCTAGGCTGGAAAATACGGGAGTTTTAGAATGCTTTGGAATTCAGGTGGGTCATACACTACTCAATTTGTGTTTCATGTGGTGTGGATTC(/G)TAGGGAACTGTTTCAGAGAGAAATAAGAGATGTAAACATAGATTATTTATGTTGATTACTCTTACACACTCAAGTAACAGAAATGATAAAGGAAAATAGAA NG 10 8 17 8 24 0 100 5 10
    250908090803_gowycfni AGGTCGGGGGAGGGGAGCCCCCCTCGGGCTGTGGTTAGAGCGGGAGAGGAACTTCCCAGACTAGCTGGCACAGAGCCTCGGGAAGGCGGCGGGCACTGCAG(/A)GTGGTTTACGGGAAGTGCTGCAGCCTTGGGGTGGGGACAGCGTGGCCAGACCCACCGCCTCATCTGCACACCTGGGCTCAAGCGCTAATGACGACAGGGGA NG 10 8 17 8 24 0 100 5 10
    250908090803_gowyc0ak GGTGGGGGTGGATGTAGCTGCAGAAAGCATGCACAACTTTGTGAAAGAGGCCCTGCCTTGTGCATGTCCATAGTGAGGCTACAGATGGCTTATTGTATATA(/C)ATTACAATGTAAATAGCTTTTTATTTCCTAAGAAATAATTTAATGTTTAGTAAAAAAGAAAACAGAAAAAAGAAAGATGCGTGTGTTGGCTTACGCACTGG NG 10 8 17 8 24 0 100 5 10
    250908090803_gowybx5t GTGGGAGGGGAGGTGGCCTGCGGAAAGGCCTGGACCTGCCCCTCACCTGGCGTCTGGCCTTGCTTCCTTGCTTTCCGGGGTCTCCTCACTGCAGCTGAGCA(/G)GGGAATGGGTAGCAGCAGCAGCTCTCGAGTGCCAGTGCGTGAACCGTTGGGGAGGTTTCCCAAAAATGCACGTTCCTCGGCCTTCTCCCCAGAGACCCGGC NG 10 8 17 8 24 0 100 5 10
    250908090803_gowyanjr TTCTCTCACACTTCATTTGTAGCAACTCAGGCCAGATGTTTTCGTCTGAAATTCTCTGGGCTTGTCATTCCACCTGTATCGTGCTGCAAACCAGGTGTTAT(/C)GCTAGTAGGCATCTGCCCTCACCCCTCTGCCTGAGTTTTCTGTAGGCAGATGTTAACCTGAGAAAACAAGTGCCATGTGGACACCAAGTAAGCATTTTTGA NG 10 8 17 8 24 0 100 5 10
    250908090803_gowy9uio GGCTGGTTTCTTTCAAGTTTAAAAAAAGCCCCCTTCTCCATGCACAAGTTCATTCCCTCCATGCTGTCGTCATCGACATATACAGGTGTGTACGTAGGTGT(/C)ATGTGTGTACTCTGCACGTATATCTCTTCTGTCTTGAAGACCCTGTTAACTTTGTGAAATAACTTCCACATTTCAACACACTTCACAGTACAGGAAATAAA NG 10 8 17 8 24 0 100 5 10