Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    260513170750_50m1 GCTTCTTGTGGACTGTTTGTTTGGCAGGTAGTAGTTCTGTACTGGACCCATCTGAGCTTCAGCCCAACTTCATTCATCGGAGGTTACATTCTCAAGAGAAAAGAGAGATGCAGAAAGAG(-ATCCTGTCC)ATACTGGGACTGAACCACAGACCCAGACCACATCTGAACAACGGCAAATATAATTCAGCACCCCTCTTCATGCTGGATCTGTACAACAGCATGTCCACTGAAGAGAAGAGCGACGTGGATCAGTACAGATCACTGTTCACCA NGG 10 8 17 8 24 0 100 5 10
    260513170603_cskk GCTTCTTGTGGACTGTTTGTTTGGCAGGTAGTAGTTCTGTACTGGACCCATCTGAGCTTCAGCCCAACTTCATTCATCGGAGGTTACATTCTCAAGAGAAAAGAGAGATGCAGAAAGAG(-ATCCTGTCC)ATACTGGGACTGAACCACAGACCCAGACCACATCTGAACAACGGCAAATATAATTCAGCACCCCTCTTCATGCTGGATCTGTACAACAGCATGTCCACTGAAGAGAAGAGCGACGTGGATCAGTACAGATCACTGTTCACCA NGG 10 8 17 8 24 0 100 5 10
    260513170329_fkb4 GCTTCTTGTGGACTGTTTGTTTGGCAGGTAGTAGTTCTGTACTGGACCCATCTGAGCTTCAGCCCAACTTCATTCATCGGAGGTTACATTCTCAAGAGAAAAGAGAGATGCAGAAAGAG(-ATCCTGTCC)ATACTGGGACTGAACCACAGACCCAGACCACATCTGAACAACGGCAAATATAATTCAGCACCCCTCTTCATGCTGGATCTGTACAACAGCATGTCCACTGAAGAGAAGAGCGACGTGGATCAGTACAGATCACTGTTCACCA NGG 10 8 17 8 24 0 100 5 10
    260513161647_v5rx TTTATTTGGCAGGTAGCAGTTCTGCACTAGACGGATCTGAGCTTCAGCCCAACTTCATCCAGCGGAGGTTGCGGACTCAGGAGAAGAGAGAGATGCAGAAAGAGATTCTG(-CCATACTGGGACTGAA)CCACAGACCCAGACCGCATCTGAACAGCGGCAAACATAATTCAGCACCCCTCTTCATGCTGGATCTGTACAACAGTATGTCCACTGAAGAGAAGAGCGATGTGGATCAGTACAGATCACTGTTCACCACCACACGACCCGCTT NGG 10 8 17 8 24 0 100 5 10
    260512205654_9kd0 ggagagctggaaagcattggggagcttttctcaagatcagtcacacatagacaactctctgaagtatatattaagtggcagaaacactcccagcttctcaaggacagtgattttagttttT(/C)aggagcctatcatggctctacgcacagtcattttggagatcctgatggaaaaggaaatggacaactcacaaagagaatgtattaaggacattctcaccaaacaccttgtagaactctctata NGG 10 8 17 8 24 0 100 5 10
    260512205616_6o6a ggagagctggaaagcattggggagcttttctcaagatcagtcacacatagacaactctctgaagtatatattaagtggcagaaacactcccagcttctcaaggacagtgattttagttttA(/G)aggagcctatcatggctctacgcacagtcattttggagatcctgatggaaaaggaaatggacaactcacaaagagaatgtattaaggacattctcaccaaacaccttgtagaactctctata NGG 10 8 17 8 24 0 100 5 10
    260512205530_vrbt ggagagctggaaagcattggggagcttttctcaagatcagtcacacatagacaactctctgaagtatatattaagtggcagaaacactcccagcttctcaaggacagtgattttagttttC(/T)aggagcctatcatggctctacgcacagtcattttggagatcctgatggaaaaggaaatggacaactcacaaagagaatgtattaaggacattctcaccaaacaccttgtagaactctctata NGG 10 8 17 8 24 0 100 5 10
    260512205417_vkmd ggagagctggaaagcattggggagcttttctcaagatcagtcacacatagacaactctctgaagtatatattaagtggcagaaacactcccagcttctcaaggacagtgattttagttttT(/A)aggagcctatcatggctctacgcacagtcattttggagatcctgatggaaaaggaaatggacaactcacaaagagaatgtattaaggacattctcaccaaacaccttgtagaactctctata NGG 10 8 17 8 24 0 100 5 10
    260512204359_7kc8 ggagagctggaaagcattggggagcttttctcaagatcagtcacacatagacaactctctgaagtatatattaagtggcagaaacactcccagcttctcaaggacagtgattttagttttT(/C)aggagcctatcatggctctacgcacagtcattttggagatcctgatggaaaaggaaatggacaactcacaaagagaatgtattaaggacattctcaccaaacaccttgtagaactctctata NGG 10 8 17 8 24 0 100 5 10
    260512171849_nyb7 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10