Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250710022028_kopi ACCCTGTGGAACCACAACTTGGCACGAGCCAATCTGCTCACAGAAGCAGGGAGGGCAGGAGGCAGGGCTGGGCATAAAAGGAAGAGCCGGGCCAGCTGCCGCTTACACTTGCTTCTGACACACCGTGCTCACTAGCAGCTGCACAAACACACAcCATGGTGCATCTGACTCCTGt(/A)GGAGAAGTCTGCCGTTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGTAGGTATCCCACTTACAAGACAGGTTTAAGGAGAGTGAATGGCACCTAGGCATGCAGGGACAGAGCTGTCCCTGAGATTCTGAAAGCTGCTGACTTCCTCTGACCTTGTGCTGTTTTCTCCCCTTAG NGG 10 8 17 8 24 0 100 7 10
    250710021827_vcs9 ACCCTGTGGAACCACAACTTGGCACGAGCCAATCTGCTCACAGAAGCAGGGAGGGCAGGAGGCAGGGCTGGGCATAAAAGGAAGAGCCGGGCCAGCTGCCGCTTACACTTGCTTCTGACACACCGTGCTCACTAGCAGCTGCACAAACACACAcCATGGTGCATCTGACTCCTGt(/A)GGAGAAGTCTGCCGTTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGTAGGTATCCCACTTACAAGACAGGTTTAAGGAGAGTGAATGGCACCTAGGCATGCAGGGACAGAGCTGTCCCTGAGATTCTGAAAGCTGCTGACTTCCTCTGACCTTGTGCTGTTTTCTCCCCTTAG NGG 10 8 17 8 24 0 100 5 10
    250709143926_404t CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250709143901_43vg CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NG 10 8 17 8 24 0 100 5 10
    250709132703_ik8i CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NG 10 8 17 8 24 0 100 5 10
    250709132634_wu5s CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250709125500_bihv CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250709125414_shri aaaaagaacaattttatcctcatccttggaagaatccattaccctatc(-a)aaggaatttggatgttaatttatgtttggataagacttgtttatccattgtc NGG 10 8 17 8 24 0 100 5 10
    250709125405_94g5 aaaaagaacaattttatcctcatccttggaagaatccattaccctatca(-a)aggaatttggatgttaatttatgtttggataagacttgtttatccattgtc NGG 10 8 17 8 24 0 100 5 10
    250709125340_sz7c aaaaagaacaattttatcctcatccttggaagaatccattaccctatcaa(-a)ggaatttggatgttaatttatgtttggataagacttgtttatccattgtc NGG 10 8 17 8 24 0 100 5 10