Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250712031134_yztj GAACTCAAATATTTTTTTTTTAACAGATTTGGTTCATTTGACTTGCACATCTTCTAAAACAGCAAGAGAAGATTTAGAATCAGTTGTGCAGAAATTGTTT(+GTTC)CATATACTGAAATGGAGATAGGTAAGAAAAATAATTGATAACTCATGATGGAAAGGTAATGTAACCATCATAATCTTCTTAGATATATCATGAAAGTTTC NG 10 8 15 10 74 0 100 10 10
    250712025511_e2d6 TTTTTAACAGATTTGGTTCATTTGACTTGCACATCTTCTAAAACAGCAAGAGAAGATTTAGAATCAGTTGTGCAGAAATTGTTT(+GTTC)CATATACTGAAATGGAGATAGGTAAGAAAAATAATTGATAACTCATGATGGAAAGGTAATGTAACCATCATAATCTTCTTAGAT NG 10 8 17 8 40 0 100 5 10
    250711205843_9gmd CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250711021608_nmzm tgccaccATGgacatctccaagtttgcagagaaagacaatatagttcttt(/g)gagaaggtggaatcacactgagtggaggtcaacgagcaagaatttctttagcaag NG 10 8 17 6 24 0 100 5 40
    250710192027_dgao CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250710182828_g5d4 AATGTGTTTTAATGCTGCCGAAAGCGTGGCGCCTGGGGGCCACCTCAAGGCCTGTTTGTCTGTTGCTTGATGCAGTCTGCGCCAGGACCCTCCCCTTCCCTGTGGTGCTGCGACCCTTATAATGAGCCTTCTTGCTTTACTCATAGGTGGGAAACTACCTCCG(/A)TATGTTCCGAGGTTCTCTGTACAAGAGATACCCCTCACTCTGGAGGCGACTAGCCACTGTGGAAGAGAGGAAGAAAATAGTTGCATCGTCACATGGTAAAAAAACAAAACCTAACACTAAGGGTGCGTCTTCACGAGGGTTTGTAAACCTGTTTCAAAACCACTCGCTTAT NGG 10 8 17 8 24 0 100 5 10
    250710152636_sx4n CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250710030503_u7co ACCCTGTGGAACCACAACTTGGCACGAGCCAATCTGCTCACAGAAGCAGGGAGGGCAGGAGGCAGGGCTGGGCATAAAAGGAAGAGCCGGGCCAGCTGCCGCTTACACTTGCTTCTGACACACCGTGCTCACTAGCAGCTGCACAAACACACAcCATGGTGCATCTGACTCCTGt(/A)GGAGAAGTCTGCCGTTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGTAGGTATCCCACTTACAAGACAGGTTTAAGGAGAGTGAATGGCACCTAGGCATGCAGGGACAGAGCTGTCCCTGAGATTCTGAAAGCTGCTGACTTCCTCTGACCTTGTGCTGTTTTCTCCCCTTAG NGG 50 8 17 8 50 30 100 7 10
    250710023451_tahz ACCCTGTGGAACCACAACTTGGCACGAGCCAATCTGCTCACAGAAGCAGGGAGGGCAGGAGGCAGGGCTGGGCATAAAAGGAAGAGCCGGGCCAGCTGCCGCTTACACTTGCTTCTGACACACCGTGCTCACTAGCAGCTGCACAAACACACAcCATGGTGCATCTGACTCCTGt(/A)GGAGAAGTCTGCCGTTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGTAGGTATCCCACTTACAAGACAGGTTTAAGGAGAGTGAATGGCACCTAGGCATGCAGGGACAGAGCTGTCCCTGAGATTCTGAAAGCTGCTGACTTCCTCTGACCTTGTGCTGTTTTCTCCCCTTAG NGG 10 8 17 8 24 30 100 7 10
    250710022139_5tzr ACCCTGTGGAACCACAACTTGGCACGAGCCAATCTGCTCACAGAAGCAGGGAGGGCAGGAGGCAGGGCTGGGCATAAAAGGAAGAGCCGGGCCAGCTGCCGCTTACACTTGCTTCTGACACACCGTGCTCACTAGCAGCTGCACAAACACACAcCATGGTGCATCTGACTCCTGt(/A)GGAGAAGTCTGCCGTTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGTAGGTATCCCACTTACAAGACAGGTTTAAGGAGAGTGAATGGCACCTAGGCATGCAGGGACAGAGCTGTCCCTGAGATTCTGAAAGCTGCTGACTTCCTCTGACCTTGTGCTGTTTTCTCCCCTTAG NGG 10 8 17 8 24 0 100 7 20