Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    251013145820_x5bs GATTCTATTCCCCAGTTAGAAAGACTGCAAACTGGTACTTGAAAACTCTGCGTTTGTCCTGTCATTCCATTTGTAGTTAAGGGTTTCACCATATGACATCCTCGGTGTTAACCAGAGGCCTTGGTGCTGATTCACAAGAACCTAAGCTGTCACTGAAATGAACTGAGAAAGTATCAGGTGGGGGGGGGGGGGAGAAGACAGCATGTTTCCATAGGAAAATGGACCAAAAGTGCAAG(+ct)TTTTGGTTCAACATTATTGAAATCCTACAGGTCATTGCTGAACGAGTCAAAGAAAGGAAGGCAGAGGAAGACTGGACGGGTGCTGGCAGGGGTCCTATCCCCTCCAAAAATAAGCGCAAGGCTTTCCTTGACTTGCTTTTGAGTGTGACTGATGAGGAAGGAAACAGATTAAGCCAGGAAGACATCCGAGAGGAAGTTGACACCTTCATGTTTGAGGTAACTTTTGTCGCCAGGTAATTTTCCT NGG 10 8 17 8 24 0 100 5 10
    251013123057_osb7 TGCATACGTGGGCTCCAACAGGTCCTCTTCCCTCCCAGTCACTGACTAACCCCGGAACCACACAGCTTCCCGTTCTCAGCTCCACAAACTTGGTGCCAAATTCTTCTCCCCTGGGAAGCATCCCTGGACACTTCCCAAAGGACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTGCAGGCCAGA(+ct)TGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCCCTGTGCCCAGCCCTGGGCTCTCTGTACATGAAGCAACTCCAGTCCCAAATATGTAGCTGTTTGGGAGGTCAGAAATAGG NGG 10 8 17 8 24 0 100 5 10
    251013122534_d8on GACAGGGCTGGGGTGGCCTCTCGTGGGGTCCAGGCCAAGTAGGTGGCCTGGGGCCTCTGGGGGATGCAGGGGAAGGGGGATGCAGGGGAACGGGGATGCAGGGGAACGGGGCTCAGTCTGAAGAGCAGAGCCAGGAACCCC(+ct)TGTAGGGAAGGGGCAGGAGAGCCAGGGGCATGAGATGGTGGACGAGGAAGGGGGACAGGGAAGCCTGAGCGCCTCTCCTGGGCTTGCCAAGGACTCAAACCCAGAAGCCCAGAGCAGGGCCTTAGGGAAGCGGGACCCTGCTCTGGGCGGAGGAATATGTCCCAGATAGCACTGGGGAC NGG 10 8 17 8 24 0 100 5 10
    251013121334_j9mq TGCATACGTGGGCTCCAACAGGTCCTCTTCCCTCCCAGTCACTGACTAACCCCGGAACCACACAGCTTCCCGTTCTCAGCTCCACAAACTTGGTGCCAAATTCTTCTCCCCTGGGAAGCATCCCTGGACACTTCCCAAAGGACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTGCAGGCCAGA(+ct)TGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCCCTGTGCCCAGCCCTGGGCTCTCTGTACATGAAGCAACTCCAGTCCCAAATATGTAGCTGTTTGGGAGGTCAGAAATAGGGGGTCCAGGAGCAAACTCCCCCCACCCCCTTTCCAAAGCCCATTCCCTCTTTAGCCAGAGCCGGGGTGTGCAGACGGCAGTCACTAGGGGGCGCTCGGCCACCACAGGGAAGCTGGGTGAATGGAGCGAGCAGCGTCTTCGAGAGTGAGGACGTGTGTGT NGG 10 8 17 8 24 0 100 5 10
    251013121126_d2w6 TGCATACGTGGGCTCCAACAGGTCCTCTTCCCTCCCAGTCACTGACTAACCCCGGAACCACACAGCTTCCCGTTCTCAGCTCCACAAACTTGGTGCCAAATTCTTCTCCCCTGGGAAGCATCCCTGGACACTTCCCAAAGGACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTGCAGGCCAGA(+a)TGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCCCTGTGCCCAGCCCTGGGCTCTCTGTACATGAAGCAACTCCAGTCCCAAATATGTAGCTGTTTGGGAGGTCAGAAATAGG NGG 10 8 17 8 24 0 100 5 10
    251013120951_1sfd GACAGGGCTGGGGTGGCCTCTCGTGGGGTCCAGGCCAAGTAGGTGGCCTGGGGCCTCTGGGGGATGCAGGGGAAGGGGGATGCAGGGGAACGGGGATGCAGGGGAACGGGGCTCAGTCTGAAGAGCAGAGCCAGGAACCCC(+a)TGTAGGGAAGGGGCAGGAGAGCCAGGGGCATGAGATGGTGGACGAGGAAGGGGGACAGGGAAGCCTGAGCGCCTCTCCTGGGCTTGCCAAGGACTCAAACCCAGAAGCCCAGAGCAGGGCCTTAGGGAAGCGGGACCCTGCTCTGGGCGGAGGAATATGTCCCAGATAGCACTGGGGAC NGG 10 8 17 8 24 0 100 5 10
    251013120929_f4yp GACAGGGCTGGGGTGGCCTCTCGTGGGGTCCAGGCCAAGTAGGTGGCCTGGGGCCTCTGGGGGATGCAGGGGAAGGGGGATGCAGGGGAACGGGGATGCAGGGGAACGGGGCTCAGTCTGAAGAGCAGAGCCAGGAACCCC(+ggaggagga)TGTAGGGAAGGGGCAGGAGAGCCAGGGGCATGAGATGGTGGACGAGGAAGGGGGACAGGGAAGCCTGAGCGCCTCTCCTGGGCTTGCCAAGGACTCAAACCCAGAAGCCCAGAGCAGGGCCTTAGGGAAGCGGGACCCTGCTCTGGGCGGAGGAATATGTCCCAGATAGCACTGGGGAC NGG 10 8 17 8 24 0 100 5 10
    251013120833_771d GACAGGGCTGGGGTGGCCTCTCGTGGGGTCCAGGCCAAGTAGGTGGCCTGGGGCCTCTGGGGGATGCAGGGGAAGGGGGATGCAGGGGAACGGGGATGCAGGGGAACGGGGCTCAGTCTGAAGAGCAGAGCCAGGAACCCC(+cttttata)TGTAGGGAAGGGGCAGGAGAGCCAGGGGCATGAGATGGTGGACGAGGAAGGGGGACAGGGAAGCCTGAGCGCCTCTCCTGGGCTTGCCAAGGACTCAAACCCAGAAGCCCAGAGCAGGGCCTTAGGGAAGCGGGACCCTGCTCTGGGCGGAGGAATATGTCCCAGATAGCACTGGGGAC NGG 10 8 17 8 24 0 100 5 10
    251013120333_q43o GACAGGGCTGGGGTGGCCTCTCGTGGGGTCCAGGCCAAGTAGGTGGCCTGGGGCCTCTGGGGGATGCAGGGGAAGGGGGATGCAGGGGAACGGGGATGCAGGGGAACGGGGCTCAGTCTGAAGAGCAGAGCCAGGAACCCC(+ctt)TGTAGGGAAGGGGCAGGAGAGCCAGGGGCATGAGATGGTGGACGAGGAAGGGGGACAGGGAAGCCTGAGCGCCTCTCCTGGGCTTGCCAAGGACTCAAACCCAGAAGCCCAGAGCAGGGCCTTAGGGAAGCGGGACCCTGCTCTGGGCGGAGGAATATGTCCCAGATAGCACTGGGGAC NGG 100 8 17 12 50 50 100 5 10
    251013104829_n0ry GGGTCCAGGCCAAGTAGGTGGCCTGGGGCCTCTGGGGGATGCAGGGGAAGGGGGATGCAGGGGAACGGGGATGCAGGGGAACGGGGCTCAGTCTGAAGAGCAGAGCCAGGAACCCC(+ctt)TGTAGGGAAGGGGCAGGAGAGCCAGGGGCATGAGATGGTGGACGAGGAAGGGGGACAGGGAAGCCTGAGCGCCTCTCCTGGGCTTGCCAAGGACTCAAACCCAGAAGCCCAGAGCAGGGCCTTAGGGAAGCGGGACCCTGCTCT NGG 10 8 17 12 50 0 100 5 10